• Title/Summary/Keyword: peptide chemical tags

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Eastern Staining: A Simple Recombinant Protein Detection Technology Using a Small Peptide Tag and Its Counter Partner Which is a Fluorescent Compound

  • Lee, Jae-Jung;Kim, Jun-Young;Zhai, Duanting;Yun, Seong-Wook;Chang, Young-Tae
    • Interdisciplinary Bio Central
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    • v.4 no.2
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    • pp.5.1-5.9
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    • 2012
  • Small peptide tags such as c-myc, HA, or FLAG tag have facilitated efficient Western-blotting of proteins of interest especially when specific antibodies for the proteins are not available. However, the conventional Western-blotting requires the multi-steps process taking at least several hours up to two days. With examples of various applications, here we show a convenient and time-saving method for protein detection which employs a fluorescent chemical BDED and its binding peptide RC-tag. And we propose "Estern staining", as a standard term for protein detection method using fluorescent chemicals and their binding small peptide tags. Eastern staining may substitutes for the time-consuming "immuno-staining" in many versatile applications.