• Microbiology and Biotechnology Letters
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• v.10 no.3
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• pp.171-175
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• 1982

An oxytetracycline as being a tetracycline-antibiotic substance displayed a general synergism in the antimicrobial activity by the interaction of ginseng saponin and antibiotics, but did not to Sarcina maginata. Penicillin G.Na as being a $\beta$-lactam-antibiotic substance displayed a synergism in the antimicrobial activities by the addition of ginseng saponin to microorganisms used, but changed the effects in the antimicrobial activity of penicillin G.Na against the genus Serratia. An antimicrobial activity by the addition of ginseng saponin to antibiotics showed a non-specificity, and it varied synergism or antagonism to Gram-positive or Gram-negative bacterium. It was assumed that a drug-resistance could be eliminated by the dual administration of ginseng saponin and antibiotics.

• Korean Journal of Veterinary Service
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• v.25 no.3
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• pp.285-294
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• 2002

The present studies were carried out to determine antibiotics residues in pork and beef muscles by EEC-4-plate and HPLC. A total of 2,534 samples of pork muscles and 1,070 samples of beef muscles from slaughter houses were collected in Gyeongnam area from January to December, 2001. The results were summarized as follows; 1. Recovery rates of TCs, Sulfa drug, Penicillin G from fortified pork and beef muscles ranged as 68.79～98.24%, 78.21～94.58% and penicillin G 63.35～67.24% respectively, by HPLC. 2. Antibiotics residues were detected in 36 sample(1.42%) of pork muscles, 29 sample (2.71%) of beef muscles by EEC-4-plate. 3. Detection rate of antibiotic residues 14 samples(0.55%) and 26 samples(2.43%), in pork and beef muscles, respectively by HPLC. Concentration of residues in 22 sample(2.06%) of beef muscle were higher than tolerance level in korea. 4. Antibiotics detected were sulfamethazine(47.37%), tetracycline(15.79%), oxytetracycline (15.79%), penicillin G(15.79%), sulfamerazine(5.26%) in pork muscle samples and oxyteracycline (37.21%), penicillin G(30.23%), sulfamethazine(20.93%), tetracycline(4.65%), sulfamerazine (2.33%), sulfadimethoxine(2.33%), sulfaquinoxine(2.33%) in beef muscle samples.

• Korean Journal of Microbiology
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• v.21 no.2
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• pp.95-102
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• 1983

The penicillin G acylase(pga) gene was cloned in the vector plasmid pKM $300(Ar^r,\;Tc^r,\;6.33kb)$ for the study of the structure and expression of the pga gene. This recombinant plasmid pPAKS-1 DNA(24.5 Kb) was cleaved into 2 fragments by restriction enzyme Eco R1.1fragment by BamH1, 4fragments by Hind III, and 2 fragments by Pst I. The pga gene was located on the Eco R1.Hind III-C fragement of pPAKS-1. The recombinant plasmids pPAKS-1 and pPAKS-2, in which the Hind III-B and Hind III-D fragments pPAKS-1 are deleted, are characterized. The results are summarized as follows : 1. Doubling times of bacterial strain bearing pPAKS-1 and pPAKS-2 are 90 and 60 minutes, respectively. 2. pPAKS-1 and pPAKS-2 are present at about 16-32 and 70 copies per cell, respectively, are 0.66 and 5.5 units, respectively, which represent 2-fold and 20-fold higher enzyme 4. pPAKS-1 is very unstable, but pPAKS-2 is stable.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.331.3-332
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• 2002

A novel penicillin G acylase (PGA)-producing bacterial strain was isolated from soil by using the Serratia marcescens overlay technique. The isolated strain was identified as Leclercia adecarboxylata based on the analyses of the biochemical characteristics (API 20E). the cellular fatty acid profile. and the 16S rDNA sequences. The gene encoding the PGA (pac gene) was cloned into the pHSG399 vector and the recombinant E. coliHB101 clones harboring the pac gene were isolated on agar plates containing phenylacetyl-L -leucine and penicillin G. (omitted)

• The Journal of the Korean Society for Microbiology
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• v.10 no.1
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• pp.13-17
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• 1975

The authors identified fourty-seven Shigella cultures among 1504 suspectable cultures of enteric pathogens collected from all over the country in 1974. Fourty-three out of fourty-seven cultures belonged to Shigella flexneri, three to Shigella sonnei and the rest to Shigella dysenteriae, and none of cultures belonging to subgroup C was detected in 1974. Three Shigella flexneri 2a cultures were isolated in Seoul area, but the others in Kangwon-Do. All the Shigella cultures were sensitive to nitrofurantoin, cephalosporin, ampicillin and penicillin G $1{\mu}g$, but resistant to bacitracin, lincomycin and penicillin V. $10{\mu}g$ by means of the In-Vitro tests.

• Journal of Microbiology and Biotechnology
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• v.26 no.5
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• pp.829-836
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• 2016

Penicillin G acylase (PGA) was immobilized on magnetic Fe₃O₄@chitosan nanoparticles through the Schiff base reaction. The immobilization conditions were optimized as follows: enzyme/support 8.8 mg/g, pH 6.0, time 40 min, and temperature 25 ℃. Under these conditions, a high immobilization efficiency of 75% and a protein loading of 6.2 mg/g-support were obtained. Broader working pH and higher thermostability were achieved by the immobilization. In addition, the immobilized PGA retained 75% initial activity after ten cycles. Kinetic parameters V_max and K_m of the free and immobilized PGAs were determined as 0.113 mmol/min/mg-protein and 0.059 mmol/min/mg-protein, and 0.68 mM and 1.19 mM, respectively. Synthesis of amoxicillin with the immobilized PGA was carried out in 40% ethylene glycol at 25 ℃ and a conversion of 72% was obtained. These results showed that the immobilization of PGA onto magnetic chitosan nanoparticles is an efficient and simple way for preparation of stable PGA.

• Korean Journal of Food Science and Technology
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• v.38 no.2
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• pp.313-316
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• 2006

To obtain antibiotic resistant profiles of Bifidobacterium, minimum inhibitory concentrations (MIC) of 14 antibiotics for 93 Bifidobacterium isolates from Korean intestine origin were determined. All strains tested were sensitive to chloramphenicol, rifampicin, and amoxicillin, whereas resistant to aminoglycoside family, nalidixic acid, and vancomycin. Among vancomycin-resistant strains, 34% were resistant at more than $100\;{\mu}g/mL$, and showed variant resistances toward tetracycline, erythromycin, and penicillin. Their resistances against penicillin, cephalothin, and tetracycline were higher than ten years ago. MIC of ten isolates from commercial yoghurt products were very similar to those of strains from Korean intestine origin, and 20% strains showed resistance at higher than $100\;{\mu}g/mL$ vancomycin. These results indicated patterns of antibiotic resistance against Bifidobacterium from Korean intestine origin and commercial yoghurts were very similar,and prevalence of vancomycin resistance for Bifidobacterium was 20%. To develop new probiotic, antibiotic resistance of vancomycin and risks involved should be evaluated.

• Journal of Microbiology and Biotechnology
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• v.11 no.5
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• pp.812-818
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• 2001

Alginate and alginate-derived oligomannuronate enhanced penicillin production in shake flask and fermentor cultures of Penicillium chrysogenum Wis 54-1255 (containing a single copy of the penicillin gene cluster) and in the high producter strain P. chrysogenum AS-P-99 (containing multiple copies of the penicillin gene cluster). Alginate was not used as a single carbon source by P. chryogenum. The stimulatory effect on penicillin production was observed in a defined medium and, to a lower extent, in a complex production medium containing corn steep liquor. Alginate-supplemented cells showed higher transcript levels of the three penicillin biosynthetic genes, pcbAB, pcbC, and penDE, than cells grown in the absence of alginate. The promoters of the pcbAB, pcbC, and penDE genes were coupled to the reporter lacZ gene and introduced as monocopy constructions in P. chrysogenum Wis 54-1225 npe10 by targeted integration in the pyrG locus; the reporter ${\beta}$-galactosidase activity expressed from the three promoters was stimulated by alginate added to the culture medium of the transformants. These results indicate that the stimulation of penicillin production by alginate was derived from an increase in the transcriptional activity of the penicillin biosynthesis genes. The induction by alginate of the transcription of the three penicillin biosynthetic genes is good example of the coordinated induction of secondary metabolism genes by elicitors of plant (or microbial) origin.

• Korean Journal of Veterinary Research
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• v.12 no.1
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• pp.85-89
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• 1972

The minimum inhibitory concentration (MIC) of five chemotherapeutic agents (penicillin, streptomycin, tetracycline, oxytetracycline and furazolidone) was measured for 126 strains of Staphylococcus aureus isolated from the udder of dairy cattle. The results obtained were as follows: 1. The MIC of penicillin, streptomycin, tetracycline, oxytetracycline and furazolidone ranged from 0.03 to 32 ug/ml, 0.06 to 128 ug/ml, 0.06 to 128 ug/ml, 1.0 to 512 ug/ml, and 0.06 to 32 ug/ml, respectively. The most frequent MIC of the above drugs were; penicillin 0.5ug/ml, streptomycin 1.0ug/ml, tetracycline 0.5ug/ml, oxytetracycline 4.0ug/ml, and furazolidone 2.0ug/ml. 2. The number of strains resistant to penicillin. streptomycin, tetracycline and oxytetracycline were 89(70.6), 9(7.1%), 10(7.9%), and 26(20.6%), respectively. Twenty-eight (29.2%) strains showed multiple resistance to more than two antibiotics tested.

• Korean Journal of Microbiology
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• v.24 no.3
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• pp.263-269
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• 1986

Membrane proteins of facultatively anaerobic Escherichia coli K12 which was logarithmically grown in aerobiosis and anaerobiosis were compared on 5 to 10% liner gradient gel electrophoresis (Na Dod $SO_4 -PAGE$). Membrane proteins were formed as different patterns between aerobiosis and anaerobiosis. Among them, 91Kdal protein (pbp1a) was not synthesized in aerobiosis and 60Kdal protein (fts cluster), in anaerobiosis. Thereby cells cultured aerobically were differenciated as diversiform cell shape, comparing cells cultured anaerobically and the latter were resistant to penicillin G. Thus it is believed that in facultative anaerobes atmospheric oxygen regulated the synthesis of membrane proteins and even the expression of equivalent genes, and moreover alleviated the resistance to an antibiotic penicillin.