• Journal of Veterinary Science
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• 제21권2호
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• pp.14.1-14.10
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• 2020

African swine fever (ASF) is a highly contagious disease of domestic pigs and wild boars (WBs). Without a vaccine, early antibody and antigen detection and rapid diagnosis are crucial for the effective prevention of the disease and the employment of control measures. In Sardinia, where 3 different suid populations coexisted closely for a long time, the disease persists since 1978. The recent ASF eradication plan involves more stringent measures to combat free-ranging pigs and any kind of illegality in the pig industry. However, critical issues such as the low level of hunter cooperation with veterinary services and the time required for ASF detection in the WBs killed during the hunting season still remain. Considering the need to deliver true ASF negative carcasses as early as possible, this study focuses on the evaluation and validation of a duplex pen-side test that simultaneously detects antibodies and antigens specific to ASF virus, to improve molecular diagnosis under field conditions. The main goal was to establish the specificity of the two pen-side tests performed simultaneously and to determine their ability to detect the true ASF negative carcasses among the hunted WBs. Blood and organ samples of the WBs hunted during the 2018/2019 hunting seasons were obtained. A total of 160 animals were tested using the pen-side kit test; samples were collected for virological and serological analyses. A specificity of 98% was observed considering the official laboratory tests as gold standards. The new diagnostic techniques could facilitate faster and cost-effective control of the disease.

• Journal of Veterinary Science
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• 제21권4호
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• pp.68.1-68.8
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• 2020

A fluorescent microsphere-based immunochromatographic strip test (FICT) was developed for the rapid, sensitive, and quantitative detection of porcine reproductive and respiratory syndrome virus (PRRSV) antibodies at the pen-side. The assay was based on the formation of a sandwich immune-complex (anti-pig IgG-PRRSV antibodies-NSP7/N), which was validated by a comparison with IDEXX-ELISA using 3325 clinical specimens. The diagnostic specificity, sensitivity, and accuracy of FICT were 97.28, 93.41, and 94.95%, respectively. FICT showed a good correlation with the virus neutralization assay. Overall, a promising pen-side diagnostic tool was developed for the rapid and quantitative detection of PRRSV antibodies within 15 min.

• Asian-Australasian Journal of Animal Sciences
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• 제18권4호
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• pp.463-469
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• 2005

An attempt was made to analyze the factors affecting mating performance of rams in pen mating systems. Due to many types of social interactions, mating performance of rams may decrease or increase. Six intact Muzaffarnagari rams were used and divided into three dominant subordinate pairs by food and ewe competition test. For dominant rams subordinate ram became competitor and vice versa. In the first experiment, ram was exposed to ewe but the competitor ram was kept outside the pen with the facility of visibility only. In the second experiment each ram was exposed to ewe along with the competitor ram and in third experiment ram was exposed to ewes (two) along with competitor ram in observation pen. Recordings of different mating and agonistic behaviour were done in all the experiments. It was found that subordinate ram's mating behaviour was inhibited by mere presence of a dominant ram out side without physical contact. However, when ram exposed to oestrus ewe(s) along with competitor ram, both dominant and subordinate rams spent much of their time in guarding activities instead of mating. In addition dominant ram tried to curtail the subordinate ram mating by agonistic interaction like fight and butting. Different guarding activities were observed either as active or passive type depending on level of dominanace. It was concluded that in a limited space the interaction of two or more rams might affect the number of services adversely.

• 한국축산시설환경학회지
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• 제16권1호
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• pp.41-50
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• 2010

본 연구 국내 여건에 적합한 돈사시설과 분뇨처리가 일체형으로 조합된 고상식 돈사를 개발하는데 있어 1차적으로 적합한 환기 시스템 선발을 하고자 수행하였다. 고상식 돈사는 가로 29m, 세로 9m, 높이 7.5m 이며, l층 높이는 3.1m, 2층 높이는 2.4m 설계 건축하였다. 각 처리별 환기시스템은 l층으로 부터 2층 중천장까지 처리별로 칸막이를 고상식 돈사 내에 설치하였다. 각 처리별 환기방식은 덕트입기$\rightarrow$측벽배기 (처리 V1), 처마입기 (측벽 천장)$\rightarrow$측벽배기 (처리 V2), 천장입기$\rightarrow$측벽배기 (처리 V3) 3개 환기방식을 조사하였다. 공기흐름도와 공기유속을 조사하였다. 공기유속 조사시 환기량은 최소, 중간, 최대로 설정하였다. 그 결과는 다음과 같다. 덕트입기에서 공기흐름도는 2층과 l 층에서 비교적 균일한 수직 하강흐름을 보였고, 처마입기 (측벽천장)는 2층과 1층에서 대칭의 공기유동 하강흐름을 보였다. 천장입기는 중천장을 따라 공기가 우산형태로 퍼지면서 배출되고 느린 공기유속 보이며 l층으로 하강흐름을 보였다 최소환기량일때 하부지점 (돈방 바닥으로부터 80 cm)의 공기유속은 덕트입기, 처마입기 그리고 천장입기 비슷한 경향이었다. 최대환기량일때 덕트입기에서 하부지점의 공기유속이 (0.10~0.26 m/s)로 변화폭이 적었고, 처마입기에서는 공기유속은(0.12~0.63 m/s)로 변화의 폭이 넓고, 천장엽 기는 공기유속이 (0.07~0.15 m/s)로 느리며 변화폭이 적었다. 이상의 결과 덕트입기를 고상식돈사에서 적용이 가능할 것으로 사료되나, 돼지 사양과 실증시험으로 좀 더 검증이 필요할 것으로 판단된다.