• Korean Journal of Food Science and Technology
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• v.45 no.5
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• pp.557-564
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• 2013

We isolated and identified antioxidants from acidic and neutral ethyl acetate fractions of the peel of pear (Pyrus pyrifolia N. cv. Chuhwangbae). We isolated 4 compounds from the methanol extract, by using 3 different types of column chromatography (Sephadex LH-20, silica gel, and octadecylsilane) and preparative HPLC. We identified the isolated compounds as (S)-(+)-2-cis-abscisic acid O-${\beta}$-D-glucopyranosyl ester (compound 1), 1-[4-O-${\beta}$-D-glucopyranosyl]phenyl ethanone (picroside, compound 2), ${\beta}$-sitosterol (compound 3), and ${\beta}$-sitosteryl 3-O-${\beta}$-D-glucopyranoside (compound 4) by nuclear magnetic resonance analysis. We are the first to report the identification of compounds 1, 2, and 4 from pear.

• Korean Journal of Plant Resources
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• v.37 no.1
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• pp.62-70
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• 2024

Fruit thinning rate and characteristics were investigated for three years on seven promising pear (Pyrus pyrifolia Nakai) cultivars, which were treated by lime-sulfur as an eco-chemical thinning substance. Lime-sulfur was treated twice at the second and third days after full bloom by cultivar. Most of pear cultivars were significantly thinned by lime-sulfur compounds. Especially 'Whangkeumbae', 'Supergold' and 'Hanareum' exhibited high flower thinning rates, 41.5%, 40.1% and 39.9%, respectively. As weather conditions at the lime-sulfur treatment, insolation and cloud amount were correlated with flower thinning rate but not significant (r = 0.49 and r = -0.45, respectively). These results suggest that lime-sulfur is suitable for reducing labor force for flower thinning of Korean pears but flower thinning effects of lime-sulfur can vary depending on other factors such as environmental conditions. This information will provide useful data for low labor force cultivation of Korean pear cultivars.

• Korean Journal of Plant Resources
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• v.35 no.6
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• pp.770-777
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• 2022

The main objective of this study was to identify the most appropriate condition for root formation of in vitro micropropagated pear (Pyrus spp.) plants. In vitro propagation was induced on Murashige and Skoog (MS) medium with 2.0 mg/L of N6-benzyladenine (BA) and 0.2 mg/L of Indole-3-butyric acid (IBA) medium. The short pre-treatment of explants with a high concentration (1 mg/L) of NAA and IBA (R0 medium) in dark for three days, followed by transfer to five different media (R1 to R5) resulted in good rooting responses in the pear 'Oharabani (P. pyrifolia × P. communis)' genotype. For the rooting experiments, the highest rooting percentage (83.3 ± 8.3%), average root length (3.6 ± 1.9 mm), total root number (31 ± 4.0), and average root number per plant (2.6 ± 2.1) were obtained on half strength (1/2) of MS medium supplemented with 30 g/L sucrose without hormones and activated charcoal (AC) (R1 medium). The highest rooting percentage was obtained at 83.3% from explants on R1 and R3 media. The rooting procedure described in this study resulted in good root formation and significantly shorting the root induction time to within 14 days of culture. Further studies are underway to test the suitability of the protocol developed in this study for other pear genotypes.

• Korean Journal of Plant Resources
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• v.36 no.6
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• pp.571-579
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• 2023

In this study, cryopreservation by droplet-vitrification was applied to pear (Pyrus spp.) germplasm. We focused on the development and assessment of various strategies for the selection of suitable tissue, osmoprotection, and dehydration. We also evaluated post-thaw recovery of cryopreserved explants by droplet-vitrification. Preferentially, we tested the effects of preculture and loading treatments to determine which tissues were more suitable, either the apical shoot tips or the axillary buds. Apical shoot tips showed the better regrowth rate than in vitro axillary buds. The most effective techniques for cryopreservation were as follows. Shoots from in vitro seedlings which had been cultured for about 5-6 weeks were cold-hardened at 4℃ for one week, excised shoot tips were precultured on liquid MS medium including 0.3 M sucrose for 31 hours and 0.7 M sucrose for 17 hours, osmoprotected in loading solution (LS) for 40 min, and then cryoprotected in dehydration solution (PVS3) for 90 min. In addition, we found that regrowth rates of explants on regrowth medium after exposure to liquid nitrogen (LN) were higher than those on MS medium. Results indicated that the highest regrowth percentage was 95.6% for 'Bartlett' cultivar and 68.9% for 'BaeYun No.3' cultivar. Consequently, apical shoot tips of two pear cultivars, 'Bartlett' (P. communis) and 'BaeYun No.3' (P. pyrifolia), were successfully cryopreserved by droplet-vitrification. Results of this study show that the enhanced droplet-vitrification method described in the present study could be used as an effective means for long-term storage of pear genetic resources.

• Horticultural Science & Technology
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• v.35 no.4
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• pp.393-401
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• 2017

One-year old pear (Pyrus pyrifolia L.) trees of a new commercial cultivar 'Manpungbae', recently developed in South Korea, were planted in 2001 (planting year 1) and trained to four trellis systems: Y-trellis, Y-II-trellis, pergola, and vase-pergola. To evaluate training systems in the local area (southern Korea), tree growth responses were compared for each trellis system from planting years 3 to 8. For trees trained to Y-trellis and pergola systems, a high proportion of land covered by tree canopy was maintained over the study duration, with 70-80% coverage in year 8. Eight-year cumulative yield per tree was increased in the vase-pergola system with a low planting density and an additional scaffold. Compared with other systems, cummulative yield use efficiency was greatest in Y-II-trellis and pergola systems in years 5 and 8. Compared with Y-II-trellis and vase-pergola systems, the 8-year cumulative yield per hectare was two fold greater for trees trained to the Y-trellis (103 tons) and pergola systems (101 tons). Use of the pergola system improved average fruit weight and fruit soluble solid contents, as well as net income in year 8. Given that we observed reduced fruit productivity and increased labor hours (pruning and orchard work), we consider the Y-trellis and pergola systems to be less valuable trellis systems than the others evaluated.

• Korean Journal of Food Science and Technology
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• v.47 no.2
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• pp.254-260
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• 2015

The effect of the extraction solvent on the physiological properties of the peel of the Korean pear (Pyrus pyrifolia cv. Niitaka) was evaluated. The total phenol content was highest in the 80%(wt) methanol extract, whereas flavonoid content was highest in the 80% ethanol extract. The antioxidant activities of the extracts were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging abilities, and their reducing power. The water and 80% methanol extracts of the pear peel had highest DPPH and ABTS radical scavenging activities, and reducing power, respectively. The inhibition of ${\alpha}$-glucosidase was highest in the 80% methanol extract, and alcohol dehydrogenase activity was highest in the water extract. All three extracts had similar antimicrobial activity. Because water, 80% ethanol, and 80% methanol extracts exhibited high activities in different assays of physiological properties, each solvent could be used for specific purposes.

• Horticultural Science & Technology
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• v.33 no.1
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• pp.18-23
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• 2015

Time-specific responses of flower bud differentiation were investigated in 'Shinko' (Pyrus pyrifolia Nakai) pear grown at different altitudes from July through December 2013 to determine their suitability as scions in a top-grafting system. Flower bud initiation and bud necrosis were monitored on each of three sections of one-year-old shoots: terminal, middle, and basal. Flower bud differentiation s tarted in September in the highlands of the Lishan area, and in J uly in the lowlands of the Zhoulan area. In Lishan, flower bud differentiation was higher in the middle and basal segments; during leaf fall, however, flower bud differentiation occurred rapidly in the terminal segment. In Zhoulan, flower buds began to differentiate from the terminal section of the shoot, and severe flower bud necrosis was noted. In July, flower buds developed normally; however, in early August, some of the buds at the basal segment showed browning. During leaf fall, some flower buds showed symptoms of necrosis with rapid and complete browning. Flower bud necrosis began at the basal segment and progressed rapidly towards middle and terminal sections. Before leaf fall, flower buds fell off when scales swelled. The terminal and middle parts of the current-year shoots, with some flower buds, collected in October or later from the Lishan area could be used as scions for top-grafting of 'Shinko' pear. Each grafting scion was a 3-5 cm shoot with one flower bud. These results suggest that scions from the terminal and middle segments of stems of 'Shinko' pear from the Lishan area can be used as scions whereas those from Zhoulan area show necrosis and might not be suitable as scions.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.290-297
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• 2015

Based on the place of its origin, pear tree (Pyrus spp.) is largely divided into European pears (P. communis, cultivated mainly in Europe and the U.S.) and Asian pears (P. pyrifolia, P. bretschneideri, and P. ussuriensis, distributed and grown in East Asian countries including China, Japan, and Korea). Most pear trees have 17 chromosomes (diploidy, 2n=2x=34). Their genetic studies and precise cultivar breeding are highly restricted by conditions such as self-incompatibility controlled by S-locus and juvenility as one major character of fruit crops. Genetic studies on Pyrus have been promoted by the development of various molecular markers. These markers are being utilized actively in various genetic studies, including genetic relationship analysis, genetic mapping, and QTL analysis. In addition, research on pear genetic linkage maps has been extended to studies for the identification of QTL for target traits such as disease resistance and genetic loci of useful traits. NGS technology has radically reduced sequencing expenses based on massive parallel reactions to enable high-capacity and high-efficiency. NGS based genome analyses have been completed for Chinese pear 'Danshansuli' and European pear 'Bartlett'. In Korea, GWAS for agricultural valuable traits such as floral structure, ripening, and total soluble contents have been conducted through resequencing. GBS has been performed for 'Whangkeumbae', 'Cheongsilri', and 'Minibae'.

• Horticultural Science & Technology
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• v.32 no.2
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• pp.193-201
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• 2014

We investigated the effect of preharvest spray of aminoethoxyvinylglycine (AVG) on the fruit quality and incidence of physiological disorders during shelf-life of 'Hanareum' and 'Wonhwang' pear (Pyrus pyrifolia Nakai). AVG applications (0, 75, 150 and $300mg{\cdot}L^{-1}$) made 30 days before anticipated harvest of respective cultivar. AVG did not influence the fruit development and quality parameters including starch content, soluble solids and acidity at harvest time in two pear cultivars although the flesh firmness of the fruits treated with $300mg{\cdot}L^{-1}$ AVG were higher than that of control in 'Hanareum' pear. Among the three AVG concentrations, only $300mg{\cdot}L^{-1}$ AVG showed effectiveness on maintaining flesh firmness in 'Hanareum' pear during shelf-life. Meanwhile, competitive high flesh firmness was attained in 'Wonhwang' pear treated with $150mg{\cdot}L^{-1}$ AVG (27.7 N) and $300mg{\cdot}L^{-1}$ AVG (28.2 N) when compared with untreated control (17.8 N) during 21 days of shelf-life at $25^{\circ}C$. AVG did not cause any significant difference at skin color development relative to control in two pear cultivars. AVG decreased ethylene production and respiration rate at $300mg{\cdot}L^{-1}$ level in two pear cultivars during the entire shelf-life. During shelf-life, low incidence of physiological disorders including internal and core browning and mealiness were attained in the fruits treated at $300mg{\cdot}L^{-1}$ AVG in two cultivars when we compared with the untreated control.

• Horticultural Science & Technology
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• v.31 no.4
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• pp.512-516
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• 2013

Pear cultivar 'Sinhwa' (Pyrus pyrifolia var. culta Nakai) was originated from a cross between 'Niitaka' and Whasan' with the aims of improving the fruit quality and the traits of cultivation and of early maturing more than 'Whasan' cultivar at Pear Research Station of National Institute of Horticultural & Herbal Science, Rural Development Administration in 1995. 'Sinhwa' was preliminarily selected in 2004 and named in 2009. The tree shows vigorous growth habit and semi-spread characters like 'Niitaka'. Furthermore, it has a sufficient and well upkeep of the flower bud, so it can be more easily cultivated in orchards. In the flower characteristics, flowering time of 'Sinhwa' is $11^{th}$ April like as maternal parent 'Niitaka'. Also 'Sinhwa' has short of pollen grains, so it is need above two pollinizer cultivars. 'Sinhwa' is highly resistant to black leaf spot (Alternaria kikuchiana) and relatively strong to pear scab (Venturia nashicola) in field condition. The optimum harvest time is around Sep. $15^{th}$ in Naju, which is ahead of 'Whasan' about 10 days in the harvest period. The fruit shape is oblate and fruit skin color is yellowish-brown during harvesting time. The average weight of fruit is 627 g, and the soluble solids content is $13.0^{\circ}Brix$. The flesh is very soft and juicy, and renders good eating quality. Shelf life is about 30 days under the room temperature condition.