• 한국약용작물학회지
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• 제28권1호
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• pp.21-28
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• 2020

Background: Owing to its high efficiency in lipid and protein production, peanut (Arachis hypogaea L.) is considered one of most important crops world-wide. The kernels of peanuts are undoubtedly the most important product this plant, whereas the skin is almost completely neglected in nutraceutical terms. However, peanut skin contains potentially health-promoting phenolics and dietary fiber, and there is considerable potential for commercial exploitation. In this study, we evaluated the α-glucosidase inhibitory activity of an extract of peanut skin (PS). Methods and Results: The α-glucosidase inhibitory effects of 80% ethanol extracts of peanut (A. hypogaea L. 'Sinpalkwang') skin were evaluated and found to have a half-maximal inhibitory concentration (IC₅₀) value of 1.2 ㎍/㎖. Progress curves for enzyme reactions were recorded spectrophotometrically, and the inhibition kinetics revealed time-dependent inhibition with enzyme isomerization. Furthermore, using ultra-high performance liquid chromatography combined with quadrupole-orbitrap mass spectrometry, we identified 26 compounds in the peanut skin extract, namely, catechin, epicatechin, and 24 proanthocyanidins. Conclusions: The results suggest that peanut skin can be utilized as an effective source of α-glucosidase inhibition in functional foods and nutraceuticals.

• 한국식품영양학회지
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• 제32권5호
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• pp.511-521
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• 2019

The purpose of this study was to investigate the inhibitory effect of enzyme activity and anti-proliferation of human cancer cell lines (HCT 116, NCI-H460 and MCF-7) of peanut skin depending on cultivars (Arachis hypogaea L. cv. K-Ol, cv. Sinpalkwang, cv. Daan, cv. Heuksaeng) and extraction solvent. Peanut skin was extracted with 80% ethanol, 80% methanol, 80% acetone, and distilled water, followed by analysis of the enzyme inhibitory activity and anticancer activity. Methanol extract of Daan cultivar most effectively inhibited ${\alpha}$-gluosidase (65.08%, 0.025 mg/mL), tyrosinase (82.49%, 2 mg/mL) and ACE (73.61%, 10 mg/mL). The inhibitory effect of peanut skin extracts on colon cancer cell (HCT-116), lung cancer cell (NCI-H460) and breast cancer cell (MCF-7) growth were investigate using MTT assay. The highest anti-proliferation of cancer cell line of peanut skin extracts was observed in the methanol extract of Daan cultivar. The cell viability on HCT 116, NCI-H460 and MCF-7 cell lines of methanol extracts from peanut skin of Daan cultivar was 48.13%, 41.03%, and 36.02% at $200{\mu}g/mL$, respectively. These results suggest that peanut skin extracts may mediate physiological activity, and provide valuable information for the use of peanut byproduct as a functional food material.

• KSBB Journal
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• 제31권1호
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• pp.14-19
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• 2016

To investigate the effect of peanut sprout extract on skin care, we measured anti-oxidant activity and whitening action. As a result of measuring DPPH radical scavenging activity to examine independent anti-oxidation of peanut sprout extract, there was strongly scavenging activity. Fluorescent material DCF-DA was used to measure hydrogen peroxide created in RAW 264.7 cells, and all concentration dependently decreased ROS production. As a result of measuring nitric oxide to examine anti-inflammation of peanut sprout extract, there was strongly inhibited nitric oxide production in RAW 264.7 cells. Tyrosinase activation was found to inhibited dose-dependant. Melanin production was also prevented dose-dependant. Therefore, it is expected to be used effectively in development of functional cosmetic materials.

• 한국작물학회지
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• 제65권3호
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• pp.248-254
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• 2020

This study was conducted to investigate the impact of harvest time on yield and seed quality in purple seed coat peanut. Compared to the average Virginia peanut variety, 'Heuksaeng', purple peanut variety has been reported to experience yield loss and reduced seed quality under delayed harvest. We examined the yield components and seed quality at different harvest times from 70 to 110 days after flowering (DAF), with harvesting occurring at intervals of 10 days. The number of mature pods per plant reached a maximum of 51.0 at 80 DAF, and average pod and seed yield peaked at 80 DAF with values of 5,229 and 3,532 kg per ha, respectively. Seed quality drastically decreased after 100 DAF, and the concentrations of two major anthocyanin compounds were significantly lower under delayed harvest. The antioxidative activities of DPPH and ABTS decreased to only 69 and 83% of their maximum values at 110 DAF. On the basis of these results, we recommend that 'Heuksaeng' is harvested approximately 10 to 15 days earlier that average Virginia-type cultivars to obtain the highest yield while minimizing the deterioration of seed quality.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 추계학술대회
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• pp.287-287
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• 2017

• 한국작물학회:학술대회논문집
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• 한국작물학회 2018년도 추계학술대회
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• pp.265-265
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• 2018

• 한국작물학회:학술대회논문집
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• 한국작물학회 2018년도 추계학술대회
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• pp.266-266
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• 2018

• 한국작물학회:학술대회논문집
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• 한국작물학회 2019년도 춘계학술대회
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• pp.178-178
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• 2019

• 한국작물학회:학술대회논문집
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• 한국작물학회 2019년도 추계학술대회
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• pp.212-212
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• 2019

• 공업화학
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• 제23권2호
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• pp.183-189
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• 2012

본 연구에서는 땅콩나물 뿌리 추출물의 세포보호 작용 및 항산화능에 관한 연구를 수행하였다. Rose-bengal로 증감된 사람 적혈구의 광용혈에 대한 땅콩나물 뿌리 추출물의 세포 보호 효과를 측정하였다. 에틸아세테이트 분획(5~50 ${\mu}g/mL$)은 농도-의존적으로 세포보호 효과를 나타냈으며, 특히 아글리콘(aglycone) 분획은 5~50 ${\mu}g/mL$의 농도 범위에서 현저한 세포보호 활성을 나타내었다. 그리고 땅콩나물 뿌리의 모든 분획은 지질 과산화 연쇄반응의 차단제인 (+)-${\alpha}$-tocopherol보다도 효과적이었다. Luminol 화학발광법을 이용한 $Fe^{3+}$-EDTA/$H_2O_2$ 계에서 생성된 활성산소종(reactive oxygen species, ROS)에 대한 땅콩나물 뿌리 추출물의 활성산소 소거활성을 측정하였다. 추출물 중 에틸아세테이트 분획($OSC_{50}$; 1.59 ${\mu}g/mL$)은 강력한 항산화제로 알려진 L-ascorbic acid (1.50 ${\mu}g/mL$)와 비교할 때 유사한 활성산소 소거활성을 보여주었다. 반면에, free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) 소거활성($FSC_{50}$)의 크기는 (+)-${\alpha}$-tocopherol > 80% MeOH 추출물 > 아글리콘 분획 > 에틸아세테이트 분획 순으로 나타났다. 이상의 결과들은 땅콩나물 뿌리 추출물이 $^1O_2$ 및 다른 활성산소종을 소거하고 활성산소종에 대항하여 세포막을 보호함으로써 생체계, 특히 태양 자외선에 노출된 피부에서 항산화제로써 작용할 수 있음을 시사한다.