• Title/Summary/Keyword: peak

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Antibacterial Activity and Component Analysis of Persicaria perfoliata Extracts (며느리배꼽 추출물의 항균 작용과 성분 분석)

  • Kim, Sun-Young;Won, Doo-Hyun;Park, Soo-Nam
    • Microbiology and Biotechnology Letters
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    • v.38 no.3
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    • pp.278-282
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    • 2010
  • In this study, the antibacterial activity and component analysis of Persicaria perfoliata extracts were investigated. MIC (Minimum Inhibitory Concentration) values of ethyl acetate fraction from P. perfoliata on Escherichia coli, Propionibacterium acnes, and Staphylococcus aureus were 0.25%, equally. The results showed that the antibacterial activity of the ethyl acetate fraction was the highest in the S. aureus and P. acnes. Ethyl acetate fraction from P. perfoliata extracts showed 5 bands in TLC and 8 peaks in HPLC (320nm). In HPLC chromatogram of ethyl acetate fraction, first main peak 2 (24.99%) and peak 3 (22.65%) were identified as quercetin-3-o-glucuronide and isoquercitrin in the order of elution time. Also, in HPLC chromatogram of deglycosylated fraction, main peak 2 (76.93%) was identified as quercetin in the order of elution time. And these components are very effective as antioxidants. Thus, these results indicate that extract/fractions of P. perfoliata can be applicable to new functional cosmetics for antioxidant, antibacterial activity.

Web Prefetching Scheme for Efficient Internet Bandwidth Usage (효율적인 인터넷 대역폭 사용을 위한 웹 프리페칭 기법)

  • Kim, Suk-Hyang;Hong, Won-Gi
    • Journal of KIISE:Information Networking
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    • v.27 no.3
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    • pp.301-314
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    • 2000
  • As the number of World Wide Web (Web) users grows, Web traffic continues to increase at an exponential rate. Currently, Web traffic is one of the major components of Internet traffic. Also, high bandwodth usage due to Web traffic is observed during peak periods while leaving bandwidth usage idle during off-peak periods. One of the solutions to reduce Web traffic and speed up Web access is through the use of Web caching. Unfortunately, Web caching has limitations for reducing network bandwidth usage during peak periods. In this paper, we focus our attention on the use of a prefetching algorithm for reducing bandwidth during peak periods by using off-peak period bandwidth. We propose a statistical, batch, proxy-side prefetching scheme that improves cache hit rate while only requiring a small amount of storage. Web objects that were accessed many times in previous 24 hours but would be expired in the next 24 hours, are selected and prefetched in our scheme. We present simulation results based on Web proxy and show that this prefetching algorithm can reduce peak time bandwidth using off-peak bandwidth.

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Determination of Rhodium by Inverted Catalytic Hydrogen peak as Analytical peak (뒤집힌 촉매수소 전류 봉우리를 이용한 로듐의 정량)

  • Kwon, Young-Soon;Lim, Kyong-Hee
    • Analytical Science and Technology
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    • v.16 no.4
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    • pp.269-276
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    • 2003
  • A new type of stripping voltammetry, inverted catalytic stripping voltammetry $IC_tSV$, is introduced. The rhodium-formaldehyde complex in hydrochloric acid gives an inverted catalytic hydrogen peak (reduction current peak during positive-going scan). The characteristics of the inverted peak were studied. By using the peak as analytical peak the detection limit of $1.2{\times}10^{-10}M$ Rh (50s preconcentration) can be reached at the optimal conditions: 0.015% (W/V) HCHO-0.42 M HCl; accumulation potential, -1.1 V; scan rate, 100 mV/s.

Structural Identification of a Non-Glycosylated Variant at Ser126 for O-Glycosylation Site from EPO BRP, Human Recombinant Erythropoietin by LC/MS Analysis

  • Byeon, Jaehee;Lim, Yu-Ri;Kim, Hyong-Ha;Suh, Jung-Keun
    • Molecules and Cells
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    • v.38 no.6
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    • pp.496-505
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    • 2015
  • A variant peak was detected in the analysis of RP-HPLC of rHu-EPO, which has about 7% relative content. Fractions of the main and the variant peaks were pooled separately and further analyzed to identify the molecular structure of the variant peak. Total mass analysis for each peak fraction using ESI-TOF MS shows differences in molecular mass. The fraction of the main peak tends to result in higher molecular masses than the fraction of the variant. The detected masses for the variant are about 600-1000 Da smaller than those for the main peak. Peptide mapping analysis for each peak fraction using Asp-N and Glu-C shows differences in O-glycopeptide profiles at Ser126. The O-glycopeptides were not detected in the fraction of the variant. It is concluded that the variant peak is non-O-glycosylated rHu-EPO and the main peak is fully O-glycosylated rHu-EPO at Ser126.

Isometric evaluation of the Lumbar extensors in Choronic Low Back Pain Patients and Healthy subjects (만성요통환자와 정상인의 요부신전근의 등척성 근력 평가)

  • Lim, Chang-Hun
    • The Journal of Korean Physical Therapy
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    • v.14 no.1
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    • pp.169-176
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    • 2002
  • The purpose of this study were to measured peak torque of lumbar extensor at various degrees and to compare with the choronic low back pain patients and healthy subjects back extensor peak torque. Research subject are fifty choronic low back pain patients and fifty healthy subjects are Dong-a university hospital visited to 2000, January since 1999, august none lumbosacral traumal past history and neurologic disorder that is twenty generation, thirty generation, forty generation, fifty generation, sixty generation in healthy subjects and twenty generation, thirty generation, forty generation, fifty generation, sixty generation in cause choronic low back pain patients. The result were as follows. 1. There were each generation choronic low back pain patients and healthy subjects back extensor peak torque are consideration (p<.05). 2. Twenty generation was all degree of angle in lumbar extention peak torque in choronic low back pain patients back extensor are lower than healthy subjects(p<.05). 3. Thirty generation was all degree of angle in lumbar extention peak torque in choronic low back pain patients back extensor are lower than healthy subjects(p<.05). 4. Forty generation was all degree of angle in lumbar extention peak torque in choronic low back pain patients back extensor are lower than healthy subjects(p<;.05). 5. Fifty generation was all degree of angle in lumbar extention peak torque in choronic low back pain patients back extensor are lower than healthy subjects(p<.05). 6. Sixty generation was all degree of angle in lumbar extention peak torque in choronic low back pain patients back extensor are lower than healthy subjects(p<.05).

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Effect of HPLC Analytical Procedure upon Determining Drug Content in PLGA Microspheres

  • Heo, Sun-Ju;Lee, Hong-Hwa;Lee, Min-Jung;Sah, Hong-Kee
    • Journal of Pharmaceutical Investigation
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    • v.40 no.3
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    • pp.193-200
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    • 2010
  • The objective of this study was to investigate the effects of sample preparation, HPLC conditions and peak measurement methods upon determining progesterone content of poly-d,l-lactide-co-glycolide microspheres. A series of the microspheres with different formulations was first prepared. To determine their actual drug contents, the microspheres were dissolved in tetrahydrofuran and diluted with various amounts of methanol to precipitate the polymer. After removal of polymeric precipitates, the filtrates were subject to HPLC analysis under versatile experimental conditions. Interestingly, the composition of a sample solution (e.g., the ratio of methanol to tetrahydrofuran) affected the magnitudes of both peak fronting and peak broadening of progesterone. Its peak became broader and more asymmetrical at lower methanol:tetrahydrofuran ratios. Furthermore, its peak height was influenced by the proportion of tetrahydrofuran in a sample solution. Such problems encountered with tetrahydrofuran were exacerbated when a larger volume of the sample solution was injected onto an analytical column. Under our experimental conditions a peak area measurement provided more accurate and reliable determination of progesterone content in various microspheres than a peak height determination. Optimizing the composition of a sample solution, HPLC chromatographic conditions and peak analysis methods was a prerequisite to an accurate determination of progesterone encapsulated within microspheres.

Daily Peak Electric Load Forecasting Using Neural Network and Fuzzy System (신경망과 퍼지시스템을 이용한 일별 최대전력부하 예측)

  • Bang, Young-Keun;Kim, Jae-Hyoun;Lee, Chul-Heui
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.67 no.1
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    • pp.96-102
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    • 2018
  • For efficient operating strategy of electric power system, forecasting of daily peak electric load is an important but difficult problem. Therefore a daily peak electric load forecasting system using a neural network and fuzzy system is presented in this paper. First, original peak load data is interpolated in order to overcome the shortage of data for effective prediction. Next, the prediction of peak load using these interpolated data as input is performed in parallel by a neural network predictor and a fuzzy predictor. The neural network predictor shows better performance at drastic change of peak load, while the fuzzy predictor yields better prediction results in gradual changes. Finally, the superior one of two predictors is selected by the rules based on rough sets at every prediction time. To verify the effectiveness of the proposed method, the computer simulation is performed on peak load data in 2015 provided by KPX.

Characterization of yeast cell wall lytic enzyme from Fusarium moniliforme (Fusarium moniliforme이 생산하는 효모세포벽 분해효소의 특성)

  • 장판식;박관화;이계호
    • Microbiology and Biotechnology Letters
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    • v.14 no.6
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    • pp.467-471
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    • 1986
  • Yeast cell wall lytic enzyme was purified from Fusarium moniliforme by ammonium sulfate fractionation and gel column chromatography. The lytic activity was found to consist of three enzyme activities which were resolved on Sephadex G-100. The first peak on chromatogram exhibited proteolytic, lytic and laminarinase activities, and the second had both lytic and laminarinase activities, whereas the third peak was shown to contain lytic activity only. Three enzyme activities showed the synergistic effect and reducing agents accelerated the yeast roil wall lysis. This indicates that lytic, proteolytic and laminarinase activity acted cooperatively in the lysis of intact cells. Tannic acid precipitate of crude enzyme constituted of three enzyme activities had a high lytic activity on viable yeast cell and has proved useful in yeast protoplast formation.

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Dependence of the peak fluxes of solar energetic particles on CME parameters and magnetic connectivity

  • Park, Jinhye;Moon, Yong-Jae;Lee, Harim;Kahler, S.W.
    • The Bulletin of The Korean Astronomical Society
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    • v.42 no.2
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    • pp.82.3-83
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    • 2017
  • We investigate the relationships between the peak fluxes of 18 solar energetic particle (SEP) events and associated coronal mass ejection (CME) 3D parameters (speed, angular width, and separation angle) obtained from SOHO, STEREO-A and/or B for the period from 2010 August to 2013 June. We apply the STEREO CME Analysis Tool (StereoCAT) to the SEP-associated CMEs to obtain 3D speeds and 3D angular widths. The separation angles are determined as the longitudinal angle between flaring regions and magnetic footpoints of the spacecraft, which are calculated by the assumption of Parker spiral field. The main results are as follows. 1) We find that the dependence of the SEP peak fluxes on CME 3D speed from multi-spacecraft is similar to that on 2D CME speed. 2) There is a positive correlation between SEP peak flux and 3D angular width from multi-spacecraft, which is much more evident than the relationship between SEP peak flux and 2D angular width. 3) There is a noticeable anti-correlation (r=-0.62) between SEP peak flux and separation angle. 4) The multiple regression method between SEP peak fluxes and CME parameters shows that the longitudinal separation angle is the most important parameter, and the CME 3D speed is secondary on SEP peak flux.

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Constitutive Model of Tendon Responses to Multiple Cyclic Demands(I) -Experimental Analysis-

  • Chun, Keyoung-Jin;Robert P. Hubbard
    • Journal of Mechanical Science and Technology
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    • v.15 no.7
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    • pp.1002-1012
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    • 2001
  • The work reported here is an extensive study of tendon response to multiple cyclic tests including 3% constant peak strain level test (A-type test), 3% constant peak strain level test with two rest periods (B-type test), and 3∼4% different peak strain level test (C-type test). A sufficient number of specimens were tested at each type of the test to statistically evaluate many changes in response during testing and differences in response between each type of the test. In cyclic tests, there were decreses (relaxations) in the peak stresses and hysteresis, increases in the slack strains, and during lower peak strain level (3%) cyclic block after higher peak strain level (4%) cyclic block in the C-type tests. Considering the results of this study and those of the other study of multiple cyclic tests with rest periods by Hubbard and Chun, 1985, recovery phenomena during the rest periods occurred predominantly at the beginning of the rest periods. Consistently in both studies, the effects of rest periods were small and transient compared to the effects of the cyclic extensions. The recovery with cycles at lower peak strain level (3%) after higher peak strain level (4%) in the C-type test has not been previously documented. This recovery seems to be a natural phenomena in tissue behavior so that collagenous structures recover during periods of decreased demand.

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