• Title/Summary/Keyword: pcbCD

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Cloning and Expression of pcbC and pcbD Genes Responsible for 2,3-Dihydroxybiphenyl Degradation from Pseudomonas sp. P20

  • Nam, Jung-Hyun;Oh, Hee-Mock;Kim, Chi-Kyung
    • Journal of Microbiology and Biotechnology
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    • v.5 no.2
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    • pp.68-73
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    • 1995
  • Pseudomonas sp. P20 was shown to be capable of degrading biphenyl and 4-chlorobiphenyl (4CB) to produce the corresponding benzoic acids wnich were not further degraded. But the potential of the strain for biodegradation of 4CB was shown to be excellent. The pcbA, B, C and D genes responsible for the aromatic ring-cleavage of biphenyl and 4CB degradation were cloned from the chromosomal DNA of the strain. In this study, the pebC and D genes specifying degradation of 2, 3-dihydroxybiphenyl (2, 3-DHBP) produced from biphenyl by the pebAB-encoded enzymes were cloned by using pBluescript SK(+) as a vector. From the pCK102 (9.3 kb) containing pebC and D genes, pCK1022 inserted with a EcoRI-HindIII DNA fragment (4.1 kb) carrying pebC and D and a pCK1092 inserted with EcoRI-XbaI fragment (1.95 kb) carrying pebC were constructed. The expression of pcbC and D' in E. coli CK102 and pebC in E. coli CK1092 was examined by gas chromatography and UV-vis spectrophotometry. 2.3-dihydroxybiphenyl was readily degraded to produce meta-cleavage product (MCP) by E. coli CK102 after incubation for 10 min, and then only benzoic acid(BA) was detected in the 24-h old culture. The MCP was detected in E. coli CK1022 containing pebC and 0 genes (by the resting cells assay) for up to 3 h after incubation and then diminished completely in 8 h, whereas the MCP accumulated in the E. coli CK1092 culture even after 6 h of incubation. The 2, 3-DHBP dioxygenases (product of pebC gene) produced by E. coli CK1, CK102, CK1023, and CK1092 strains were measured by native PAGE analysis to be about 250 kDa in molecular weight, which were about same as those of Pseudomonas sp. DJ-12, P. pseudoa1caligenes KF707, and P. putida OU83.

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Pollution Status for Agricultural Reservior in Chungbuk Province (충북지역 농업용 저수지의 오염실태 조사연구)

  • Oh, Kwang-Young;Kim, Jin-Soo;Song, Chang-Sub;Kwon, Sung-Ill;Song, Chul-Min
    • Proceedings of the Korea Water Resources Association Conference
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    • 2007.05a
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    • pp.987-992
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    • 2007
  • 충청북도 내의 농업용 저수지중 26개소를 선정하여 관개초기, 홍수기 및 비관개기의 연 3회에 걸쳐 저수율을 파악하고 채수를 실시하였다. 또한, 현장(수온, pH, DO, EC)과 실내 수질분석(Cd, As, Cn, Hg, 유기인, Pb, $Cr^{+6}$ PCB, ABS, COD, SS, TP, TN)을 실시하여 저수지의 오염도를 평가하였다. 저수율 측정결과, 홍수기(97%)가 관개초기(72%)나 비관개기(66%)보다 높게 나타났다. pH 값의 측정결과, 수질기준$(6.0{\sim}8.5)$을 만족하고 있는 저수지의 비율은 35%(9개소)의 낮은 값을 보였다. EC와 DO 및 SS농도의 측정결과, 거의 모든 저수지에서 수질기준을 만족하고 있었으며, COD, TN, TP농도 측정결과, 수질기준 만족도는 관개초기 100%, 홍수기 81%, 비관개기 65%로 나타나, 저수량이 많은 관개초기보다 저수량이 적은 비관개기에 질소와 인의 관리가 필요한 것으로 판단된다. 또한, 사람의 건강항목(CN, Hg, 유기인, PCB, Cd, 비소As, Pb, $Cr^{6+}$, ABS)은 모든 저수지에서 관개 초기, 홍수기 및 비관개기에 모두 수질기준을 만족하였다. 점오염원을 관리하기 위해서는 저수지 상류유역에 있는 마을하수도를 정비하고, 축산분뇨처리시설 등을 설치하여 저수지로의 유입부하량을 저감할 필요가 있다. 또한, 비점원오염을 관리하기 위한 대책으로서, 질소농도를 저감시키기 위해서는 용존성 성분을 제공하는 비료의 시용량을 줄여야하며, TP농도를 저감시키기 위해서는 강우시 입자성 인의 유출을 제어할 필요가 있으며, 이를 위해서는 비가 많이 오는 여름철에 나지(裸地)나 밭에 식생이나 멀칭(mulching) 등으로 토양침식을 방지할 필요가 있다.가용한 장 단기간의 관측자료와 물수지 분석 연산식의 추정치를 바탕으로 관측자료에 의한 물수지 분석을 수행하였다. 분석 결과로 산지 소하천 유역인 설마천 시험유역의 각 수문요소의 물이동간의 정량적인 값을 알 수가 있었으며, 앞으로 추가적이고 지속적인 수문모니터링이 운영되고 물순환 해석 모형에 의한 검증이 수행된다면 정량적인 물순환 관계를 규명할 수 있을 뿐만 아니라 이와 관련된 수문요소기술을 확보할 수 있을 것이다.절한 타협과 조정을 필요로 한다. 그러나 절제의 한계를 넘어선다고 생각되거나, 조정의 노력이 불가능하거나, 실패했을 때 폭력적인 행동으로 나타나게 된다. 리차즈(I.A Richards)는 분노와 공포는 일단 겉잡을 수 없는 경향이 있다고 하면서 오늘날 폭력에 대한 요구가 일상의 정서 생활에 있어, 억압을 통한, 빈곤함을 반영하고 있지 않은지 생각해봐야 할 것이라고 충고한다. 조성 가이드라인(안)을 제시하였다.EX>$\ulcorner$세종실록$\lrcorner$(世宗實錄) $\ulcorner$지리지$\lrcorner$(地理志)와의 비교를 해보면 상 중 하품의 통합 9개소가 삭제되어 있고, $\ulcorner$동국여지승람$\lrcorner$(東國與地勝覽) 에서는 자기소와 도기소의 위치가 완전히 삭제되어 있다. 이러한 현상은 첫째, 15세기 중엽 경제적 태평과 함께 백자의 수요 생산이 증가하자 군신의 변별(辨別)과 사치를 이유로 강력하게 규제하여 백자의 확대와 발전에 걸림돌이 되었다. 둘째, 동기(銅器)의 대체품으로 자기를 만들어 충당해야할 강제성 당위성 상실로 인한 자기수요 감소를 초래하였을 것으로 사료된다. 셋째, 경기도 광주에서 백자관요가 운영되었으므로 지방인 상주지역에도 더 이

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On the Harzadous Components of Paper Mill Sludge and Sludge Treated Soil (제지(製紙)슬러지와 슬러지 시용토양중(施用土壤中)의 유해성분(有害性分)에 관하여)

  • Lee, Kyu Seung;Choi, Jong Woo;Jo, Jeong Rye;Kim, Moon Kyu
    • Korean Journal of Agricultural Science
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    • v.19 no.2
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    • pp.194-200
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    • 1992
  • In order to use organic resource like a compost 44 samples of paper mill sludge were collected from each paper manufacturer. Some metal and PCBs(polychlorinated biphenyls) contents in/on sludge were investigated and metal contents of soil treated with kraft and paper board sludge were checked in contrast io the non-treated soil. The staple results were the same as followings. 1. Average contents of Pb in sludge ranged 14.5-25.7 ppm, and Al, Cd, Cu, Fe, Zn were similar concentration to unpolluted soil. Also, Cr and Hg were not detected from any sludge sample. 2. PCBs contents in sludge of kraft, paper board and news print paper were determined. Less than 10 ppb of $PCB_s$ contents were detected from kraft and paper board sludges, but a little more was found in sludge of news print paper. 3. Some metal contents in sludge treated soil were much similar to non-treated soil and the concentration also were ranged under the average of unpolluted natural soil. 4. From these results paper mill sludge seemed to be no harmful effects to soil.

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몇 가지 PBTs (Persistent, Bioaccumulative, Toxic Chemicals)가 생태계 곤충에 미치는 영향

  • Lee Seun Yeong;Kim Yong Gyun
    • Proceedings of the Korea Society of Environmental Biology Conference
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    • 2002.11a
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    • pp.123-126
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    • 2002
  • Pollutants that are persistent, bioaccurnulative, and toxic have been linked to numerous adverse effects in human and animals, PBTs include heavy metals, polychlorinated biphenyls (PCBs), dioxins, polycyclic aromatic compounds (PACs) in addition to pesticides. This study focuses on toxic effects of the PBTs except pesticides on insects. Eight PBTs were selected from subgroups: three heavy metals (Pb, Hg, and Cd), two PCB mixtures (Aroclor mixtures 1 and 2), 2,3,7,8-tetrachlorodibenzo-p-dioxin, two monophenols (4-octylphenol and 4-nonylphenol), and tetrabutyltin, Beet armyworm, Spodoptera exigua, was used as test target insect species. Three physiological markers (metamorphosis, immune reaction, and follicle patency) were assessed in each exposure to different doses of the PCBs. Heat-shock proteins as molecular markers were also analyzed in response to the PCBs. All tested PBTs were toxic to metamorphosis from larvae to pupae when they were applied with diet. Two PCB mixtures were the most toxic compounds in this assay by giving significant toxicity at 0.005 ppm, while others had from 10 to 1000 ppm. Dioxin (0.1 ppb), tetrabutyltin (0.1 ppb), Pb (10 ppb), and Hg (0,01 ppb) were potent to inhibit immune reactions analyzed by inducing phenoloxidase activity and blocked phospholipase $A_2$ enzyme, Tetrabutyltin and dioxin significantly induced follicle cell patency, but their effects were lower than that of endogenous juvenile hormone, Dioxin, Pb, Hg, and Cd could induce the expression of heat shock proteins that were detected by immunoblotting against human HSP70 monoclonal antibody. HSP78 and HSP80 were upregulated in response to the PBTs. This expression was detected from the fat body and epidermis at as fast as 4h after injection. All these results clearly suggest that PBTs give significant ecotoxicity to insects that are valuable organisms in our environment.

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A Study on the Thermal and Optical Properties of a LED Chamber Light for Vessels (선박용 LED Chamber Light의 열 및 광학 특성에 관한 연구)

  • Kim, Sang-Hyun;Lee, Do-Yup;Kim, Woo-Sung;Jang, Nakwon
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.28 no.1
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    • pp.57-63
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    • 2015
  • Recently, LED is widely used in the kinds of display devices or lighting. In this paper, we fabricated LED chamber light for naval vessels to replace to conventional chamber light using incandescent lamp. The LED package of chamber light was designed with luminous intensity of 5.5 cd, color temperature of $6,000{\pm}500K$, forward voltage of 3~3.2 V and input current of 60 mA. A LED module was composed of 36 LED packages and metal PCB. The VF and luminous intensity of LED package were getting down when temperature increased. The temperature of LED chamber light was measured by changing the number of LED package and applied current for one hour when an electric current flow. The heat transfer capability have been improved by using metal PCB. The power consumption of LED chamber light reduced by 86% compared to the conventional chamber light using incandescent lamp.

Determinations of Environmental Hormones and Heavy Metals in Seawater of Tongyeong Marine Ranching Ground of Korea (통영 바다목장 해역의 해수 내 환경호르몬과 중금속 분석)

  • Hahn, Young-Hee;Lee, Jung-Sun;Lee, Jin-Hwan
    • Korean Journal of Environmental Biology
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    • v.25 no.4
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    • pp.313-318
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    • 2007
  • Monitoring for the quality of coastal water is necessary to improve its biological resources and to maintain healthy environments. We measured the concentrations of phenols, nine polychlorinated biphenyls (PCBs), tributyltin (TBT) in surface water at 5 stations and the concentrations of mercury (Hg), chromium (Cr (VI)), cadmium (Cd), lead (Pb) in surface water of 10 stations in Tongyeong marine ranching ground of Korea in August, 2003. The concentrations of analytes were determined as follows: phenols: 1.6$\sim$2.8 ppb, PCBs: not detected (ND), Hg: <0.1 ppb, Cr (VI): 0.01$\sim$5.32 ppb, Cd: ND$\sim$0.41 ppb and Pb: 0.43$\sim$2.60 ppb. These concentrations satisfied the standards of human health protection in coastal water by Ministry of Environment, Republic of Korea as follows: phenols; 5 ppb, PCBs: 0.5 ppb, Hg: 0.5 ppb, Cr (VI): 50 ppb, Cd: 10 ppb and Pb: 50 ppb. TBT was not listed in the standards. The concentrations of Sn in TBT analyzed in sea water of Tongyeong marine ranching ground were in the range of ND$\sim$0.0273 ppb which are similar with the values of 8$\sim$35 ng Sn $L^{-1}$ (0.008$\sim$0.035 ppb) in Chinhae Bay studied by Shim et al.. Therefore, the quality of sea water in Tongyeong marine ranching ground was safe enough to protect human health.

Assessment of Environmental Pollution in Korean Stream Sediments by Chemical Analyses and Insect Immune Biomarkers

  • Ryoo, Keon-Sang;Byun, Sang-Hyuk;Hong, Yong-Pyo;Cho, Ki-Jong;Bae, Yeon-Jae;Kim, Yong-Gyun
    • Korean Journal of Environmental Biology
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    • v.26 no.4
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    • pp.330-342
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    • 2008
  • A comprehensive quality survey for PCDDs/PCDFs and coplanar PCBs as well as heavy metals (Cu, Zn, Cd and Pb) in sediments has been investigated in August 2006, Korea. Monitoring was undertaken at five streams representing different surrounding environments throughout Juwang and Gapyeong streams (reference sites), Jungrang stream (dense population site), Ansan stream (mixed small population and industrial site), and Siheung stream (heavy industrial site). The levels of heavy metal in samples were found to be significantly higher in sediment from Siheung stream compared to those of other stream sites. The heavy metal concentrations (dry weight basis) in sediment from Siheung stream were as follows; Cd (3.7 ${\mu}g$/g), Pb (1,295 ${\mu}g$/g), Cu (713.4 ${\mu}g$/g) and Zn (358.1 ${\mu}g$/g). Among 12 coplanar PCBs and 17 PCDDs/PCDFs selected as target compounds in this study, PCB (IUPAC no. 118) and OCDD were the most abundant congeners found in all sediment samples, followed by 1,2,3,4,6,7,8-HpCDD, OCDF and 1,2,3,4,6,7,8HpCDF as well as PCB (IUPAC no. 105). These results were shown to be in the same trend as the sediment samples of other countries. The levels of PCDDs/PCDFs/coplanar PCBs in sediment samples were expressed as concentrations and WHO- TEQ values. The PCDDs/PCDFs/coplanar PCBs concentrations and their WHO-TEQ values in sediment from Siheung stream were remarkably high. The levels detected were 788.16 pg/g and 36.080 pg WHO-TEQ/g dry weight for PCDDs/ PCDFs and 314 pg/g and 0.4189 pg WHO-TEQ/g dry weight for coplanar PCBs, respectively, beyond the safety level of sediment value 20 pg WHO-TEQ/g. Sediment samples of the five streams were also monitored by sensitive biomarkers using insect immune responses: hemocyte-spreading behavior and immune-associated enzyme activities of phospholipase A$_2$ (PLA$_2$) and phenoloxidase. Organic extracts of Siheung and Jungrang sediments significantly interfered with the hemocytespreading behavior, whereas those of Ansan, Gapyeong, and Juwang did not. These organic extracts did not inhibit the PLA$_2$ and phenoloxidase activities. However, phenoloxidase was highly susceptible to exposure to aqueous extracts in all site sediments. In comparison, PLA$_2$ activities of the hemocytes were significantly inhibited only by aqueous extracts of Siheung, Jungrang, and Gapyeong sediments, but not by those of Ansan and Juwang. Despite some disparity between bioand chemical monitoring results, the biomarkers can be recommended as a device warning the contamination of biohazard environmental chemicals because of a fast and inexpensive detection method.

Environmental Pollutants in Streams of Andong District and Insect Immune Biomarker (안동지역 하천의 환경오염물질과 곤충면역 생체지표 분석)

  • Ryoo Keon Sang;Ko Seong-Oon;Cho Sunghwan;Lee Hwasung;Kim Yonggyun
    • Korean journal of applied entomology
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    • v.44 no.2
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    • pp.97-108
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    • 2005
  • Samples of water, soil, and sediment were taken from 10 streams near Andong, Korea in May 2004. To assess the degree of environmental pollution of each stream, chemical pollutants such as total notrogen (T-N), total phosphorus (T-P), chemical oxygen demand (COD), heavy metals, organophosphorus pesticides, organochlorine pesticides, and dioxin-like PCB congeners were analyzed by standard process tests or U.S. EPA methods. In addition, biomarkers originated from insect immune systems of beet armyworm, Spodoptera exigua, were used to analysis of the environmental samples. Except Waya-chun stream showing T-N content of 9.12 mg/L, most streams were contaminated with relatively low levels of overall pollutants in terms of T-N, T-P, and COD, compared to their acceptable environmental levels designated by the Ministry of Environment. Contents of Pb and Cd in samples of each stream were much lower than environmentally permissible levels. However, several times higherconcentrations of Pb and Cd were found in locations at Mi-chun, Kilan-chun, and Hyunha-chun streams, in comparison with other streams. Diazinon, parathion, and phenthoate compounds among organophosphorus pesticides were detected as concentrations of 0.19, 0.40, and $1.13\;{\mu}g/g$, respectively, from soil sample collected in the vicinity of Mi-chun stream. On the other hand, 16 organochlorine pesticides and 12 dioxin-like PCB congeners, known as endocrine disrupting chemicals, selected in this study were not found above the limit of detection. Biomarker analyses using insect immune responses indicated that Waya-chun stream was suspected as exposure to environmental pollutants. Limitation and compensation of both environmental analysis techniques are discussed.

Chemical Characterization of Commercial Dark-fleshed Fishes (Mackerel Scomber japonicus, Japanese Spanish mackerel Scomberomorus niphonius, Pacific herring Clupea pallasii) as a Raw Material for Seafood Products (수산식품가공소재로서 시판 적색육 어류[고등어(Scomber japonicus), 삼치(Scomberomorus niphonius), 청어(Clupea pallasii)]의 화학적 위생특성)

  • Kang, Young Mi;Park, Sun Young;Lee, Su Gwang;Lee, Jung Suck;Heu, Min Soo;Kim, Jin-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.2
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    • pp.130-138
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    • 2017
  • This study examined chemical characterization in commercial dark-fleshed fish (mackerel Scomber japonicus, Japanese Spanish mackerel Scomberomorus niphonius, and Pacific herring Clupea pallasii) to determine their suitability for use as raw materials for seafood products. The volatile basic nitrogen (VBN), heavy metal, radioactivity, polychlorinated biphenyl (PCBs), benzo[a]pyrene, and histamine concentrations were measured. The VBN in all of the dark-fleshed fish was less than 20 mg/100 g, which is the limit for raw materials for seafood processing. Except for mackerel based on the lead (Pb) standards of the European Union (EU), Taiwan, and CODEX, and all fish based on the cadmium (Cd) standards for China and the EU, the commercial dark-fleshed fish were free from total mercury (Hg), Pb, and Cd based on domestic and foreign standards. The radioactivity, polychlorinated biphenyl (PCB), benzo[a]pyrene, and histamine concentrations of the commercial dark-fleshed fish all adhered to the domestic and foreign standards. The commercial dark-fleshed fish tested could all be used as raw materials for seafood products, except for some exported products.

Structural and Functional Stability of the Genetic Recombinant Plasmid pCU103 in Different Water Environments

  • Kim, Chi-Kyung;Kwak, Myoung-Ja;Lee, Sung-Gie
    • Journal of Microbiology
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    • v.34 no.3
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    • pp.241-247
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    • 1996
  • The stability of the genetically engineered microorganisms and their recombinant plasmids released in natural environments has been regarded as one of the molecular ecological topics. In this study, the recombinant plasmids pCU103 in which the pcbCD genes involved in biodegradation of biphenyl and 4-chlorobiphenyl were cloned in pBluescript SK(+) vector, were examined for their structural and functional stability in different waters at 15 $^{\circ}C$ by the methods of electrophoresis, Southern hybridization, quantification with fluorescent dye, and transformation. The recombinant plamids maintained their stabilities for about 30 days in sterilized distilled water (SDW), 15 days in autoclaved creek water (AW), 25 days in filtered and autoclaved non-sterile creek water (FAW), 4 days in Luria-Bertani (LB) broth, and less than one day in filtered non-sterile creek water (FW). The covalently closed circular (CCC) form of the plasmid was decreased and open circular (OC) form was increased as a function of incubation time, and then linear (L) form was produced to be ultimately degraded out. The degradation rates of the plasmid were proportionally correlated to trophic level of the water, and the biological factor such as DNases was found to be one of the most critical factors affecting structural and functional stability of the plasmid in non-sterile natural water.

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