• 대한의생명과학회지
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• 제28권4호
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• pp.298-306
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• 2022

Pathological tissue fixation using formalin has been widely used for histological samples in many hospitals and institutions. In general, formalin fixatives were either manufactured in laboratories or purchased commercially because of the risks and environmental concerns of handling organic compounds. In this study, the efficacy of three kinds of commercially purchased and one laboratory-made formalin fixative was compared in the PCR-based molecular diagnosis using the extracted DNA from formalin-fixed paraffin-embedded (FFPE) tissues. The quality of extracted DNA from FFPE tonsil tissues with four kinds of formalin solutions was evaluated, and PCR for beta-globin gene and microsatellite instabilities (MSI) tests for pentaplex panel markers were performed using the extracted DNA. There was no difference in PCR and MSI tests as molecular diagnoses regardless of the types of formalin used in this study. However, the total amount and average length of double-stranded DNA extracted from FFPE tonsil tissue showed significant differences according to the type of formalin fixative. Optimized formalin fixatives and methods for DNA extraction might be sophisticated to extract good quality DNA from the small size of specific tissue samples. Further studies are needed to select the most effective formalin fixative for histology and molecular pathology using human FFPE tissues.

• The Journal of Korean Physical Therapy
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• 제14권4호
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• pp.367-384
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• 2002

Cerebral palsy refers to the neuromuscular deficit caused by nonprogressive defect or lesion in single or multiple locations in the immature brain resulting in Impaired motor function and sensory integrity. The pathophysiological events may occur during the prenatal intrapartum, perinatal, or early postnatal period. Cerebral palsy is the most common condition and it poses a challenge to practitioners due to the large variation in prognosis for motor function of children with this diagnosis. The objectives of this article are review to pathological and psychological factors of cerebral palsy and upper extremity function. Upper extremity and hand function are most important in activity of daily living in cerebral palsy This article hope to give the information for application in many therapists.

• Journal of Digestive Cancer Research
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• 제11권1호
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• pp.15-20
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• 2023

Endoscopic submucosal dissection (ESD) is a minimally invasive and effective treatment for early gastric cancer. The criteria for determining the suitability and success of ESD have expanded, with changes in the histopathological classification of gastric carcinoma, including results from molecular research. Treatment methods have also diversified to include targeted therapies and immuno-oncology agents. To improve communication between clinicians and pathologists, it is crucial to understand the standardized diagnostic forms of gastric cancer. This study aims to examine the handling method of ESD specimens and describe the pathological findings of gastric tumors.

• Journal of Chest Surgery
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• 제33권2호
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• pp.179-182
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• 2000

Background: A pleural effusion is not a disease entity but a clincial sign of systemic or pleural disease. Although the diagnosis of pleural effusion can often be done by toracentesis and pleural needle biopsy the yeild of positive diagnosis is low.

• Journal of Preventive Medicine and Public Health
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• 제29권4호
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• pp.877-897
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• 1996

In recent years, the Korean Medical Association has undertaken the feat of establishing the Korean Standard Terminology of Medical Procedures with the dedicated help of 32 medical academic societies. However, because the project is being conducted by several different circles, it has yet to see a clear system of classification. This thesis, therefore, proposes the three principles of scientific properties, usefulness and ideology as the basis for classification system and has developed the Classification System of Medical Procedures in Korea upon their foundation. The methodology and organization of this thesis as follows. First, by adopting scientific classification system of Feinstein(1988), an analysis of the classification systems of the medical procedures in the United States, Japan, Taiwan, WHO was carried out to reveal the framework and the basic principles in each system. Second, the direction of classification system has been constructed by applying the normative principle of medical field in order to show the future direction of the medical field and realize its ideology. Third, a finalized framework for the classification system will be presented as based on the direction of classification system. Of the three basis principles mentioned above, the analysis on the principles of usefulness was left out of this thesis due to the difficulty of establishing specific standards of analysis. The results of the study are as follows. The overall structure of the thesis is aimed at showing the 'Prevention-Therapy-Rehabilitation' quality of comprehensive health care and consists of six chapters; I. Prevention and Health Promotion II. Evaluation and Management III. Diagnostic Procedures IV. Endoscopy V. Therapeutic Procedures VI. Rehabilitation Chapter three Diagnostic Procedures is divided into four parts : Functional Diagnosis, Visual Diagnosis, Pathological Diagnosis, Biopsy and Sampling. Chapter five Therapeutic Procedures is divided into Psychiatry, Non-Invasive Therapy, Invasive Therapy, Anaesthesia and Radiation Oncology. Of these sub-divisions, Functional Diagnosis, Biopsy and Sampling, Endoscopy and Invasive Therapy employs the anatomical system of classification. On the other hand, Visual Diagnosis, Pathological Diagnosis, Anesthesia and Diagnostic Radiology, namely those divisions in which there is little or no overlapping in services with other divisions, used the classification system of its own division. The classification system introduced in this thesis can be further supplemented through the use of the cluster analysis by incorporating the advice and assistance of other specialists.

• Asian Pacific Journal of Cancer Prevention
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• 제17권5호
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• pp.2649-2653
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• 2016

Background: Genetic alterations in gliomas have increasing importance for classification purposes. Thus, we are especially interested in studying IDH mutations which may feature potential roles in diagnosis, prognosis and response to treatment. Our aim was to investigate IDH mutations in diffuse glioma patients diagnosed in university hospital centre of Fez in Morocco. Materials and Methods: IDH1 codon 132 and IDH2 codon 172 were direct-sequenced in 117 diffuse glioma samples diagnosed and treated in University Hospital Hassan II between 2010 and 2014. Results: The R132H IDH1 mutation was identified in 43/117 tumor samples and R172K IDH2 mutation was detected in only one anaplastic oligodendroglioma. IDH mutations were observed in 63.2% of astrocytomas, 73.3% of diffuse oligodendrogliomas and 12.90% of glioblastomas. Conclusions: Our results confirmed other studies published earlier for other populations with some small discrepancies.

• Parasites, Hosts and Diseases
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• 제55권3호
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• pp.267-285
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• 2017

Angiostrongylus cantonensis invades the central nervous system (CNS) of humans to induce eosinophilic meningitis and meningoencephalitis and leads to persistent headache, cognitive dysfunction, and ataxic gait. Infected mice (nonpermissive host), admittedly, suffer more serious pathological injuries than rats (permissive host). However, the pathological basis of these manifestations is incompletely elucidated. In this study, the behavioral test, histological and immunohistochemical techniques, and analysis of apoptotic gene expression, especially caspase-3, were conducted. The movement and motor coordination were investigated at week 2 post infection (PI) and week 3 PI in mice and rats, respectively. The cognitive impairs could be found in mice at week 2 PI but not in rats. The plaque-like lesion, perivascular cuffing of inflammatory cells, and dilated vessels within the cerebral cortex and hippocampus were more serious in mice than in rats at week 3 PI. Transcriptomic analysis showed activated extrinsic apoptotic pathway through increased expression of TNFR1 and caspase-8 in mice CNS. Immunohistochemical and double-labeling for NeuN and caspase-3 indicated the dramatically increased expression of caspase-3 in neuron of the cerebral cortex and hippocampus in mice but not in rats. Furthermore, western-blotting results showed high expression of cleaved caspase-3 proteins in mice but relatively low expression in rats. Thus, extrinsic apoptotic pathway participated in neuronal apoptosis might be the pathological basis of distinct behavioral dysfunctions in rodents with A. cantonensis infection. It provides the evidences of a primary molecular mechanism for the behavioral dysfunction and paves the ways to clinical diagnosis and therapy for A. cantonensis infection.

• 정신신체의학
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• 제21권2호
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• pp.93-98
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• 2013

병적 웃음과 울음(Pathological laughing and crying, PLC)은 감정과 관계없이 갑작스럽게 표출되는 웃음이나 울음이 특징이며 신경계 질환과 관련 있다. 이는 환자의 대인관계 및 일상생활에 상당한 곤란을 가져오지만 약물치료에 비교적 반응이 좋으므로 적절한 진단이 중요하다. PLC는 전전두엽과 변연주변 네트워크(paralimbic network), 소뇌 및 교뇌 기저의 조절이상으로 발생한다. 이들의 조절에는 여러 신경전달물질이 관여하며 특히 세로토닌성 기능의 이상이 관련있다. 주된 치료는 약물치료이며 선택적 세로토닌 재흡수 억제제를 비롯한 항우울제에 좋은 효과를 보인다. 그 외 도파민계 약물, 비경쟁적 NMDA 수용체 길항제도 일부 효과가 있다. PLC의 진단과 평가에는 몇 가지 척도들이 사용되고 있으나 그 특징적 임상양상을 파악하는 것이 가장 중요하다. 따라서 이러한 PLC의 임상양상과 병태생리를 이해하고 적절한 치료법을 살펴봄으로써 임상에서의 관심을 높이고자 한다.

• 사상체질의학회지
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• 제24권3호
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• pp.50-59
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• 2012

Objectives In this research, the constitutional diagnosis based on the theory of Sasang constitutions were carried out to Japanese subjects living in Japan, and the external applicability of Sasang constitutional medicine was evaluated by analyzing the physiological symptoms and the pathological symptoms according to their constitutions. Methods Total 144 Japanese subjects who live in S city were invited from Mar. 2010 to Jul. 2011 for the research, and their constitutions were analyzed with constitution diagnosing tool that was developed by Korean institution of oriental medicine (KIOM) in 2011. The collected data was analyzed in terms of the physiological symptoms and the pathological symptoms according to the sasang constitutions with computer software of SPSS 17.0. Results In the physiological symptoms related to excrements, Soyangin more frequently did not have excrements of gold color (p=.034) and feel the tenesmus (p=.047) than the subjects of other constitutions. In the physiological symptoms related to cold & heat, Soyangin more frequently felt cold in feet and hands (p=.011), and Soeumin more frequently didn't like drinking water regardless of thirst (p=.017). In the physiological symptoms, Soyangin frequently felt dryness in eyes, and Taeumin frequently felt uncomfortable because of the phlegm. Conclusions The theory of Sasang constitution which is a Korean traditional medicine can also be applied to the health care of Japanese, and it can contribute to the health promotion of Japanese subjects.

• 구강회복응용과학지
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• 제38권1호
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• pp.26-33
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• 2022

전치부 치간 사이 공간은 치주 환자에게 흔히 발생되는 병적 치아 이동(PTM, pathological tooth migration)과 함께 자주 관찰된다. PTM에는 다양한 교합적 요인들이 기여하는데 특히 상악 전치부에서는 과도한 교합력이 있을 때 위치 문제로 인한 불리함 때문에 치간 이개가 쉽게 발생한다. 치주 지지 조직이 소실된 치아는 의도적인 연결 고정(splinting)을 통해 지지 면적을 확장하면 안정성이 증가될 수 있다. 또한 고정성 보철물로 수복 시에는 불리한 교합을 유리한 형태로 바꿀 수 있다. 임시보철물을 통해 안정적 교합을 확보 후 이것을 최종보철물로 옮기는 일은 CAD-CAM을 통하여 가능하다. 본 증례에서는 치간 접촉이 상실된 상악 전치부에서 정확한 진단과 치료를 통해 장기적으로 안정적인 결과를 보여 소개하고자 한다.