• Title/Summary/Keyword: partitioning order

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Partitioning of Recombinant Human Interleukin-2 in a Poly(ethylene glycol)-Dextran Aqueous Two-Phase System

  • Lee, In-Young;Lee, Sun-Bok
    • Journal of Microbiology and Biotechnology
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    • v.2 no.2
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    • pp.135-140
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    • 1992
  • The partitioning of recombinant human interleukin-2(rhII-2) in PEG 8000-dextran 38800 aqueous two-phase system has been investigated using three different sources of rhIL-2. In the case of pure rhIL-2, the solubility in a PEG-dextran two-phase system was low and most of rhIL-2 was partitioned into the bottom phase. For the recovery of rhIL-2 from insoluble protein aggregates, the inclusion bodies of recombinant E. coli were solubilized by the treatment with sodium dodecyl sulfate (SDS). The addition of SDS significantly enhanced not only the solubility of rhIL-2 but also the partitioning of rhIL-2 to the top phase. When the ratio of SDS to rhIL-2 was 2.0, the partition coefficient(K) and the recovery yield(Y) at the top phase were 4.5 and 88%, respectively, at pH 6.8. In order to reduce the recovery steps further, SDS was directly added to the intact recombinant E. coli cells and then partitioned into the PEG/dextran aqueous two-phase system. The observed partition coefficient ($K{\cong{3.0$) and recovery yield ($Y{\geq}80%$ )of this method were comparable to the rhIL-2 recovery from insoluble protein aggregates. The results obtained in this work indicate that PEG-dextran two-phase partitioning might provide a simple way for the recovery and partial purification of recombinant proteins which are produced as inclusion bodies.

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Effect of n-6/n-3 fatty acid ratio on metabolic partitioning in hyperlipidemic rats (n-6/n-3 지방산 비율이 고지혈증 랫드의 지질대사 분할에 미치는 영향)

  • Lee, Seunghyung;Um, Kyung-Hwan;Park, Byung-Sung
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.3
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    • pp.643-653
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    • 2018
  • This study was investigated the mechanism of action of n-6/n-3 fatty acid ratio on the metabolic partitioning of blood glycerolipids by in vivo monitoring technique in hyperlipidemic animal model rats. The ratio of cholesteryl 14C-oleate metabolized in the liver of total glycerolipids was lower in the order of n-6/n-3 ratios of 4:1, 15:1, 30:1 and control group (p<0.05). The secretion amount of phospholipid was higher in the order of n-6/n-3 ratio 4:1, 15:1, 30:1 than the control (p<0.05). The secretion amount of triglyceride was lower in especially 4:1, in order of n-6/n-3 4:1, 15:1 and 30:1 compared with the control. The ratio of phospholipid partitioning to total glycerolipid was high in orfer of n-6/n-3 ratio 4:1, 15:1, 30:1 and control (p<0.05). The triacylglycerol partitioning (%) via liver was higher 72.97, 75.93, 78.12% in n-6/n-3 4;1, 15:1, 30:1, respectively than the control of 82.25%, according to increased n-6/n-3 (p<0.05). The phospholipid partitioning (%) was lower 25.15, 18.87, 18.15% in n-6/n-3 4;1, 15:1, 30:1, respectively, compared to control 11.04%, according to increased n-6/n-3 (p<0.05).

Spatial Partitioning using filbert Space Filling Curve for Spatial Query Optimization (공간 질의 최적화를 위한 힐버트 공간 순서화에 따른 공간 분할)

  • Whang, Whan-Kyu;Kim, Hyun-Guk
    • The KIPS Transactions:PartD
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    • v.11D no.1
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    • pp.23-30
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    • 2004
  • In order to approximate the spatial query result size we partition the input rectangles into subsets and estimate the query result size based on the partitioned spatial area. In this paper we examine query result size estimation in skewed data. We examine the existing spatial partitioning techniques such as equi-area and equi-count partitioning, which are analogous to the equi-width and equi-height histograms used in relational databases, and examine the other partitioning techniques based on spatial indexing. In this paper we propose a new spatial partitioning technique based on the Hilbert space filling curve. We present a detailed experimental evaluation comparing the proposed technique and the existing techniques using synthetic as well as real-life datasets. The experiments showed that the proposed partitioning technique based on the Hilbert space filling curve achieves better query result size estimation than the existing techniques for space query size, bucket numbers, skewed data, and spatial data size.

A Variant of the Brillouin-Wigner Perturbation Theory with Epstein-Nesbet Partitioning

  • Lee, Sangyoub;Choi, Cheol Ho;Kim, Eunji;Choi, Young Kyun
    • Bulletin of the Korean Chemical Society
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    • v.34 no.11
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    • pp.3279-3283
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    • 2013
  • We present an elementary pedagogical derivation of the Brillouin-Wigner and the Rayleigh-Schr$\ddot{o}$dinger perturbation theories with Epstein-Nesbet partitioning. A variant of the Brillouin-Wigner perturbation theory is also introduced, which can be easily extended to the quasi-degenerate case. A main advantage of the new theory is that the computing time required for obtaining the successive higher-order results is minimal after the third-order calculation. We illustrate the accuracy of the new perturbation theory for some simple model systems like the perturbed harmonic oscillator and the particle in a box.

Free vibration analysis of uniform and stepped functionally graded circular cylindrical shells

  • Li, Haichao;Pang, Fuzhen;Du, Yuan;Gao, Cong
    • Steel and Composite Structures
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    • v.33 no.2
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    • pp.163-180
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    • 2019
  • A semi analytical method is employed to analyze free vibration characteristics of uniform and stepped functionally graded circular cylindrical shells under complex boundary conditions. The analytical model is established based on multi-segment partitioning strategy and first-order shear deformation theory. The displacement functions are handled by unified Jacobi polynomials and Fourier series. In order to obtain continuous conditions and satisfy complex boundary conditions, the penalty method about spring technique is adopted. The solutions about free vibration behavior of functionally graded circular cylindrical shells were obtained by approach of Rayleigh-Ritz. To confirm the dependability and validity of present approach, numerical verifications and convergence studies are conducted on functionally graded cylindrical shells under various influencing factors such as boundaries, spring parameters et al. The present method apparently has rapid convergence ability and excellent stability, and the results of the paper are closely agreed with those obtained by FEM and published literatures.

Hierarchical Measurement System Design by System Partitioning (계통 분할에 의한 계층적 측정 시스템 설계)

  • 문영현;최상봉;박영문;추진부
    • The Transactions of the Korean Institute of Electrical Engineers
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    • v.37 no.5
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    • pp.261-271
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    • 1988
  • This paper presents a hierarchical algorithm of the measurement system design by system partitioning. With the increase of system size, the conventional algorithms of the optimal measurement system design confront the problems of excessive memory requirements, long computation time and cumulative computation errors. In order to overcome these problems, a hierarchical approach by system partitioning is proposed with the introduction of equivalent measurements for all the extemal measurements. This approach has the advantage of remarkable reduction in computation time and memory requirements, and guarantees sufficient calculation accuracy in its application to large power systems. The proposed algorithm has been tested for various systems, which shows its applicability to practical power systems.

Performance Analysis of a Cellular Networks Using Power Control Based Frequency Reuse Partitioning

  • Mohsini, Mustafa Habibu;Kim, Seung-Yeon;Cho, Choong-Ho
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.40 no.3
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    • pp.559-567
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    • 2015
  • This paper focuses on evaluating the performance of a cellular network using power control based frequency reuse partitioning (FRP) in downlink (DL). In our work, in order to have the realistic environment, the spectral efficiency of the system is evaluated through traffic analysis, which most of the previous works did not consider. To further decrease the cell edge user's outage, the concept of power ratio is introduced and applied to the DL FRP based cellular network. In considering network topology, we first divide the cell coverage area into two regions, the inner and outer regions. We then allocate different sub-bands in the inner and outer regions of each cell. In the analysis, for each zone ratio, the performance of FRP system is evaluated for the given number of power ratios. We consider performance metrics such as call blocking probability, channel utilization, outage probability and effective throughput. The simulation results show that there is a significant improvement in the outage experienced by outer UEs with power control scheme compared to that with no power control scheme and an increase in overall system throughput.

A Partition Technique of UML-based Software Models for Multi-Processor Embedded Systems (멀티프로세서용 임베디드 시스템을 위한 UML 기반 소프트웨어 모델의 분할 기법)

  • Kim, Jong-Phil;Hong, Jang-Eui
    • The KIPS Transactions:PartD
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    • v.15D no.1
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    • pp.87-98
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    • 2008
  • In company with the demand of powerful processing units for embedded systems, the method to develop embedded software is also required to support the demand in new approach. In order to improve the resource utilization and system performance, software modeling techniques have to consider the features of hardware architecture. This paper proposes a partitioning technique of UML-based software models, which focus the generation of the allocatable software components into multiprocessor architecture. Our partitioning technique, at first, transforms UML models to CBCFGs(Constraint-Based Control Flow Graphs), and then slices the CBCFGs with consideration of parallelism and data dependency. We believe that our proposition gives practical applicability in the areas of platform specific modeling and performance estimation in model-driven embedded software development.

A Study on the Solid Waste Collection Districting and Vehicle Routing-Scheduling for Waste Collection Using GIS (GIS를 이용한 생활폐기물의 수거권역설정과 수거차량의 순회경로계획에 관한 연구)

  • 이희연;임은선
    • Spatial Information Research
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    • v.9 no.1
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    • pp.15-30
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    • 2001
  • Solid waste collection service is viewed as one of the most important public services in urban area. The purpose of this study is to apply the GIS based regional partitioning and arc routing methods for solid waste collection districting and vehicle routing-scheduling in order to provide waste collection service more efficiently. In this study, solid waste deposit sites are derived from the centroid of each building and the amount of solid waste is deduced based on the number of households and establishments. The regional partitioning procedure is performed based on waste collection zones which are constructed from waste deposit sites. The result of this study shows that solid waste collection districts which are delineated by regional partitioning method are able to increase efficiencies and cut costs in performing solid waste collection services. Also the output of vehicle-scheduling from the analysis of arc routing may provide more efficiently and quickly manage the scheduling of the residential solid waste collection routes, reducing costs with minimal deadheading costs. Therefore, the application of GIS based on regional partitioning and arc routing methods would be very useful to construct a solid waste management system by supplying the important and flexible informations for solid waste collection districts and vehicle routing-scheduling for waste collection.

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TCAM Partitioning for High-Performance Packet Classification (고성능 패킷 분류를 위한 TCAM 분할)

  • Kim Kyu-Ho;Kang Seok-Min;Song Il-Seop;Kwon Teack-Geun
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.31 no.2B
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    • pp.91-97
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    • 2006
  • As increasing the network bandwidth, the threat of a network also increases with emerging various new services. For a high-performance network security, It is generally used that high-speed packet classification methods which employ hardware like TCAM. There needs an method using these devices efficiently because they are expensive and their capacity is not sufficient. In this paper, we propose an efficient packet classification using a Ternary-CAM(TCAM) which is widely used device for high-speed packet classification in which we have applied Snort rule set for the well-known intrusion detection system. In order to save the size of an expensive TCAM, we have eliminated duplicated IP addresses and port numbers in the rule according to the partitioning of a table in the TCAM, and we have represented negation and range rules with reduced TCAM size. We also keep advantages of low TCAM capacity consumption and reduce the number of TCAM lookups by decreasing the TCAM partitioning using combining port numbers. According to simulation results on our TCAM partitioning, the size of a TCAM can be reduced by upto 98$\%$ and the performance does not degrade significantly for high-speed packet classification with a large amount of rules.