• Journal of Korean Society of Steel Construction
• /
• v.22 no.4
• /
• pp.355-363
• /
• 2010

The objective of this study is to examine the structural performance on the Rectangular Steel Tube Column-to-H Beam connections using one-side bolts and T-stub. Although a rectangular steel tube comparing with a H-shaped steel has many advantages and is more efficient, its application is limited due to the lack of experiences and connection details. Existing steel moment connections using the rectangular steel tube are mainly using through plate diaphragms. Its processing of construction is so complicated that it is hard to apply in the field. In this study, the structural performance and the earthquake capacity for T-stub connection with one-side bolts were investigated. And it is performed a comparative analysis of strength, rigidity, total rotation and energy absorption capacity for the various connection details.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.19 no.1
• /
• pp.714-721
• /
• 2018

In this study, we investigate a design technology intended to radically increase the buckling strength of vertically curved panels. Recent studies proposed a buckling strength formula which properly reflects the effect on the local plate buckling strength of flat plates when they are stiffened by closed section ribs. Herein, we attempted to quantitatively evaluate this effect on curved panels and to reveal the correlations with the design parameters. The commercial finite element software, ABAQUS, was used to build a three dimensional numerical model and numerical parametric studies were conducted to evaluate the variation of the buckling strength. In the case of flat panels, the local buckling strength of stiffened curved panels increases proportionally with increasing rotational stiffness of the closed-section ribs. After attaining a limiting value, an obvious tendency was found that the local buckling strength of the stiffened curved panel would converge towards a fixed value when the panels are supported along both sides. The parametric studies performed using the influential design parameters confirmed that the estimated partially-restrained curved panel strength is well correlated with the proposed formula.

• Journal of Korean Society of Steel Construction
• /
• v.29 no.4
• /
• pp.269-280
• /
• 2017

In this study, seismic performance was evaluated for the exposed column-base plate weak-axis connections of small size steel structures through cyclic loading tests. The primary test parameters are the thickness of base plate, the presence of rib plates, the number of anchor bolts and embedment length of anchor bolts. To investigate the effect of bond performance of anchor bolts on the seismic performance of column-base plate connections, L-shaped round bars and thread bars were used as the hooked anchor bolts in the test specimens. Test results showed that bond performance of anchor bolts and the thickness of base plate significantly affect the structural performance and energy dissipation capacity. In particular, it was found that even if the requirements for minimum thickness of the base plate that is satisfied, the base plate can yield before the capacity of steel column reaches the plastic moment resulting in decreasing the structural performance of the connections. However, the proposed details of the connections might be considered as the partially restrained, that is semi-rigid connections. Consequently, the L-shaped thread anchor bolts is applicable in the exposed column-base plate weak-axis connections of small-size steel structures.

• Journal of Periodontal and Implant Science
• /
• v.27 no.2
• /
• pp.379-394
• /
• 1997

Several methods have been used for regeneration of tissue lost by periodontal disease. Subepithelial connective tissue graft technique, one of the technniques of mucogingival surgery, is used for the regeneration in esthetic problems such as recession, and denuded root coverage. This study is performed to evaluate the healing process and the regeneration and reattachment of periodontal tissue, including the reconstruction of junctional epithelium, and connective tissue. Alveolar defects in five adult dogs were treated with periodontal surgery and were attained by removing the marginal alveolar bone by $4{\time}3mm$ from CEJ in the labial side of incisors, and root surfaces were planed. The experimental sites were divided into two groups as follows. 1. root planing alone(control group) 2. with connective tissue graft (Experimental Group) In the two groups flaps were positioned and sutured tightly, the healing processes were observed and were histologically compared with each other after 2days, 4days, 1week, 2weeks, 4weeks. The results were obtained as follows : 1. In the two groups blood clots were observed as early as 2 and 4 days, and were resorbed at 1 week. 2. In the two groups moderate inflammation was observed as early as 2 and 4 days, decreased at 1 and 2 weeks, and disappeared at 4 weeks. 3. Junctional Epithelium migration was more significant in the control group, and was restrained by graft materials in the experimental group. 4. Features of connective tissue fiber attachment partially showed the parallel pattern in the two groups from 2 weeks, and entirely from 4weeks. 5. Anastomosis, between graft and connective tissue, appeared from 4 days in the experimental group and the border between them was not discriminated at 4weeks.

• Food Science and Biotechnology
• /
• v.15 no.1
• /
• pp.63-69
• /
• 2006

The primary objective of this study is to determine whether a diet supplemented with brown rice koji (BRK) results in a reduced stress response in rats and mice. BRK, which has been suggested as a candidate for use as a stress- and fatigue-fighting supplement, was compared with red ginseng extract (RG) for its stress-reducing potential. The animals in this study were divided into no-stress, stress, RG, and BRK groups of 8 to 10 animals each. Stress was induced by means of immobilization (being restrained in plastic tubes for 30 min and electroshock (0.5 mA in mice or 2 mA in rats for 5 min). The no-stress group was not exposed to stress. Rats in the RG group received oral doses of 200 mg RG extract/kg body weight daily. The BRK group was fed a 30% BRK diet and exposed to stress. Animals were given supplements for 7 days before being exposed to stress, and then were given supplements for 5 days with exposure to stress. When the stress exposure ended, the animals were observed for stress-related changes in behavior and their plasma corticosterone levels were measured. BRK supplementation was associated with a partial blockade of the effects of stress on locomotion and elevated plus-maze test results in rats and mice. It was also associated with a partial reduction in stress-induced behaviors such as freezing, burrowing, smelling, face-washing, and rearing. BRK supplementation did not have a significant effect on plasma corticosterone levels, which were increased in the animals exposed to stress (p<0.01). The mice in the RG group received RG in water (2 mg RG/ mL $H_2O$), and the BRK group received a 30% BRK diet (weight) for 7 days. Both groups were evaluated for signs of fatigue. BRK supplementation increased endurance, as indicated by time on the rota-rod, in cold water, and on the horizontal wire. These results suggest that BRK supplementation partially protects the animal from the effects of stress and may also contribute to resistance to fatigue on physical exertion.

• International Journal of Stem Cells
• /
• v.15 no.4
• /
• pp.359-371
• /
• 2022

Background and Objectives: The goal of this study was to investigate the mechanism of mesenchymal stem cell (MSC)-derived microRNA (miR)-150-5p-expressing exosomes in promoting skin wound healing through activating PI3K/AKT pathway by PTEN. Methods and Results: Human umbilical cord (HUC)-MSCs were infected with miR-150-5p overexpression and its control lentivirus, and HUC-MSCs-derived exosomes (MSCs-Exos) with stable expression of miR-150-5p were obtained. HaCaT cells were induced by H₂O₂ to establish a cellular model of skin injury, in which the expression of miR-150-5p and PTEN and the phosphorylation of PI3K and AKT were evaluated. HaCaT cells were transfected with pcDNA3.1-PTEN or pcDNA3.1 and then cultured with normal exosomes or exosomes stably expressing miR-150-5p. Cell proliferation was inspected by CCK-8. Cell migration was detected by scratch test and cell apoptosis by flow cytometry. The starBase tool was used to predict the binding site of miR-150-5p to PTEN. Dual-luciferase reporter assay and RIP assay were applied to assess the interaction between miR-150-5p and PTEN. In H₂O₂-induced HaCaT cells, the miR-150-5p expression decreased, and PTEN expression increased in a concentration-dependent manner. MSCs-Exos promoted the growth and migration of H₂O₂-induced HaCaT cells and inhibited their apoptosis. In addition, overexpression of exosomal miR-150-5p enhanced the protective effect of MSCs-Exos on H₂O₂-induced HaCaT cells; PTEN overexpression in HaCaT cells partially restrained miR-150-5p-mediated inhibition on H₂O₂-induced injury in HaCaT cells. PTEN was a target gene of miR-150-5p. MiR-150-5p regulated PI3K/AKT pathway through PTEN. Conclusions: MSCs-derived miR-150-5p-expressing exosomes promote skin wound healing by activating PI3K/AKT pathway through PTEN.