• 제목/요약/키워드: partial identification

검색결과 371건 처리시간 0.028초

Multi-loci Molecular Characterisation of Endophytic Fungi Isolated from Five Medicinal Plants of Meghalaya, India

  • Bhagobaty, Ranjan Kumar;Joshi, S.R.
    • Mycobiology
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    • 제39권2호
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    • pp.71-78
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    • 2011
  • The phylogenetic relationships of the most dominant and morphologically cryptic endophytic fungal isolates from each of five selected medicinal plants, namely Potentilla fulgens, Osbeckia stellata, Osbeckia chinensis, Camellia caduca, and Schima khasiana of the biodiversity rich state of Meghalaya, were assessed with random amplification of polymorphic DNA and PCR-restriction fragment length polymorphism profiles. Sequencing of the internal transcribed spacer 1, small subunit rRNA and partial ${\beta}$-tubulin gene fragments was also conducted to determine the phylogenetic relationships of these isolates with fungal sequences available in Genbank, NCBI. The identity of the fungal isolates is suggested based on the molecular phylogenetic data.

전자코를 이용한 휘발성분의 분석과 식품에의 이용 (Analysis of Volatile Compounds using Electronic Nose and its Application in Food Industry)

  • 노봉수
    • 한국식품과학회지
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    • 제37권6호
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    • pp.1048-1064
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    • 2005
  • Detection of specific compounds influencing food flavor quality is not easy. Electronic nose, comprised of electronic chemical sensors with partial specificity and appropriate pattern recognition system, is capable of recognizing simple and complex volatiles. It provides fast analysis with simple and straightforward results and is best suited for quality control and process monitoring of flavor in food industry. This review examines application of electronic nose in food analysis with brief explanation of its principle. Characteristics of different sensors and sensor drift. and solutions to related problems are reviewed. Applications of electronic nose in food industry include monitoring of fermentation process and lipid oxidation, prediction of shelf life, identification of irradiated volatile compounds, discrimination of food material origin, and quality control of food and processing by principal component analysis and neural network analysis. Electronic nose could be useful for quality control in food industry when correlating analytical instrumental data with sensory evaluation results.

An Empirical Study of Relationships among IT Capability, Trust, and Attitude on RFID Adoption in Korea

  • Lim, Se-Hun;Kim, Soh-Young;Kim, Jin-Soo
    • 디지털융복합연구
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    • 제7권1호
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    • pp.99-109
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    • 2009
  • Recently, many enterprises are interest in implementing Radio Frequency IDentification (RFID). However, they have some difficulty in implementing RFID because of incompleteness of RFID technology and uncertainty of Return on Investment (ROI). Even though usefulness of RFID are recognized, many enterprises are just interested in planning of RFID rather than implementation of RFID. Among successful factors of RFID implementation, Information Technology (IT) capability is the most important one. If enterprises have systematic IT capability, it would make positive attitude to implement RFID. In addition, it will provide trust about RFID and promote adoption of RFID implementation. This study, therefore, empirically analyzed the relationships of trust, attitude, IT capability, and intention to RFID adoption using Partial Least Squares (PLS) approach. The result show useful guidelines and practical implication in implementing RFID.

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폐쇄성 수면 무호흡증 환자의 상기도 검사법 (Upper Airway Studies in Patients with Obstructive Sleep Apnea Syndrome)

  • 김정수;이규엽
    • 수면정신생리
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    • 제11권1호
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    • pp.5-9
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    • 2004
  • Obstructive sleep apnea (OSA) is a common disorder characterized by recurrent cessation of breathing due to complete or partial upper airway occlusion during sleep. The incompetent tone of palatal, pharngeal, and glossal muscles which fail to maintain airway patency during sleep causes narrowing of the airway dimension and increased resistance of breathing. The identification of the sites of upper airway obstruction in patients with OSA is important in understanding the pathogenesis and deciding the treatment modality of snoring and/or OSA. Various upper airway imaging modalities have been used to assess upper airway size and precise localization of the sites of upper airway obstruction during sleep. Dynamic imaging modalities enabled assessment of dimensional changes in the upper airway during respiration and sleep. This article focused on reviews of various upper airway imaging modalities, especially dynamic upper airway imaging studies providing important information on the pathogenesis of OSA.

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Identification of Arbuscular Mycorrhizal Fungi from Botrychium ternatum Native in Korea

  • Lee, Jun-Ki;Eom, Ahn-Heum;Lee, Sang-Sun
    • Mycobiology
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    • 제32권4호
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    • pp.179-185
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    • 2004
  • Arbuscular mycorrhizal fungi were observed in Botrychium ternatum native in Korea. The partial small subunit(SSU) of ribosomal DNA gene from the fern roots was amplified with primers AM1/NS31. Nucleotides sequence analysis of the clones revealed that two fragments were close to Glomus proliferum and G. sinuosum. The other three DNA fragments were close to those of G. proliferum with the relatively low similarities($92{\sim}95%$) and speculated to be originated from three different species of Glomus(GLA006, GLA016, and GLA032). Five different nucleotide sequences close to three AM fungal species were found in the roots of B. ternatum native in Korea.

Synthesis and Structure Identification of ABCH Type Calix[4]arenes: Two Step Synthesis of Asymmetrically Substituted Calix[4]arenes from Monoalkylcalix[4]arenes

  • 남계천;김종민;박영자
    • Bulletin of the Korean Chemical Society
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    • 제19권7호
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    • pp.770-776
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    • 1998
  • Several ABCH type chiral calix[4]arenes were prepared from monoalkyl calix[4]arenes by treating with various acyl halide, followed by reacting with benzoyl chloride in pyridine. These asymmetrically substituted ABCH type calix[4]arenes are obtained as racemates mixture which are confirmed by the chiral shift reagent in 1H NMR spectra. The molecular and crystal structure of 5-nitro-26-allyloxy-25-benzoyloxy-28-isobutyryloxy-27-hydroxycalix[4]arene 8a has been determined by the X-ray diffraction method. Two independent enantiomeric molecules are crystallized in a 1: 1 racemate mixture. They are in the partial cone conformation in which the benzoyloxy phenyl group is down. There is a bifurcated intramolecular hydrogen bonding involving three functional groups in each molecule.

Molecular Characterization of Filenchus cylindricus (Thorne & Malek, 1968) Niblack & Bernard, 1985 (Tylenchida: Tylenchidae) from Korea, with Comments on Its Morphology

  • Mwamula, Abraham Okki;Kim, Yiseul;Kim, Yeong Ho;Lee, Ho-wook;Kim, Young Ho;Lee, Dong Woon
    • The Plant Pathology Journal
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    • 제38권4호
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    • pp.323-333
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    • 2022
  • Filenchus cylindricus (Thorne & Malek, 1968) Niblack & Bernard, 1985 was reported from the sandy rhizospheric soils of Poa pratensis and for the first time in Korea. Females and males are molecularly characterized and morphological and morphometric data supplied. Identification was made using an integrative approach considering morphological characteristics and inferences drawn from the analyses of the D2-D3 expansion segment of 28S rRNA and ITS1-5.8S-ITS2 of rRNA partial sequences. Females and males from Korea conform to the type descriptions and also to subsequent species descriptions from Iowa and Colorado USA, Sudan and Pakistan. Despite the close morphological and morphometric similarities with F. thornei (Andrássy, 1954) Andrássy, 1963, the two species can be adequately differentiated based on molecular data inference.

First Record of Scolelepis (Scolelepis) daphoinos (Annelida: Polychaeta: Spionidae) in South Korea

  • Lee, Geon Hyeok;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
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    • 제37권3호
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    • pp.229-234
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    • 2021
  • Scolelepis (Scolelepis) daphoinos is newly reported in Korean fauna. This species can be distinguished from its congeners by the following characteristics: the presence of reddish pigment patches on the posterior part of the prostomium, notopodial postchaetal lamellae that are partially fused to the branchiae, and the presence of only the bidentate hooded hooks. The morphological diagnosis and photographs of S. (S.) daphoinos are provided. The partial mitochondrial cytochrome c oxidase subunit I (COI), 16S ribosomal DNA(16S rDNA), and the nuclear 18S ribosomal DNA (18S rDNA) sequences from Korean specimens of S. (S.) daphoinos were determined. Species identification was supported by a comparison of DNA barcode sequences of COI and 16S rDNA with morphological examination from the specimens of type locality, China.

Identification and Characterization Colletotrichum spp. Causing Mango Dieback in Indonesia

  • Khaerani Nurlaelita;Arif Wibowo;Ani Widiastuti
    • The Plant Pathology Journal
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    • 제40권4호
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    • pp.399-407
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    • 2024
  • Dieback disease in mango trees has been observed in Indonesia, particularly in Java Island, with the causal agent remaining unidentified. One of the important pathogens that are responsible for causing mango dieback is Colletotrichum. Field surveys were conducted in various mango cultivating areas in Java Island, Indonesia to assess prevalence of Colletotrichum as dieback disease pathogen. Eleven Colletotrichum isolates were recovered from symptomatic dieback twigs and morphologically characterized. Genetic diversity fingerprint analysis was carried out using rep-PCR. Phylogenetic analysis identified isolates as belonging to Colletotrichum asianum and Colletotrichum cairnsense using partial sequences of four gene regions, including ITS, ACT, GAPDH, and TUB2. Pathogenicity tests on mango seedlings cv. Arumanis showed that all fungal isolates were responsible for causing dieback symptoms. Subsequently, symptomatic tissue was reisolated to fulfill Koch's Postulate. This study represented new funding for two species of Colletotrichum causing mango dieback in Indonesia.

해양변사자 신원확인을 위한 해양경찰의 인체유래물 보관사업 모델제시 (Korea Coast Guard's Human Biological Materials Storage Project for Identifying Bodies Recovered from the Sea: A Model Suggestion)

  • 주현정;추민규;백윤기;김남율;최아진;임선영;이종남;김형규;이한성
    • 해양환경안전학회지
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    • 제24권2호
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    • pp.171-178
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    • 2018
  • 해양경찰은 바다라는 극한 환경에서 임무수행 중 빈번히 사망 실종의 위험에 맞닥뜨린다. 사고 발생 시 신속한 신원확인을 위해 해양경찰연구센터는 해양경찰을 대상으로 DNA를 이용한 신원확인 시스템을 구축하였으며 사체가 발견되지 않을 경우를 대비해 국립묘지 안장이 가능한 장치 또한 마련하였다. 순직 실종 등 대비 신원확인용 인체유래물 보관사업 운영규칙에 따라 인체유래물의 관리, 보관, 폐기, 품질검사가 진행되며 이는 관리위원회의 감시 하에 행해진다. 한반도 해역에서는 매년 700여건의 해양 변사자가 발생하는데 사체가 늦게 발견되어 부패가 심하거나, 일부분만 발견되어 지문 또는 치아로 개인 식별이 불가능할 경우 해양 변사자의 신원확인을 위한 방법이 필요하다. 해양경찰연구센터가 운영중인 '인체유래물 보관사업'을 해양 수산 종사자, 관련 연구자, 해양 레저인에게까지 확대 실행한다면 해양 변사자 신원확인에 큰 도움이 될 것이다.