• Journal of Animal Science and Technology
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• v.64 no.4
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• pp.800-811
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• 2022

The integration of metabolomics and transcriptomics may elucidate the correlation between the genotypic and phenotypic patterns in organisms. In equine physiology, various metabolite levels vary during exercise, which may be correlated with a modified gene expression pattern of related genes. Integrated metabolomic and transcriptomic studies in horses have not been conducted to date. The objective of this study was to detect the effect of moderate exercise on the metabolomic and transcriptomic levels in horses. In this study, using nuclear magnetic resonance (NMR) spectroscopy, we analyzed the concentrations of metabolites in muscle and plasma; we also determined the gene expression patterns of branched chain (alpha) keto acid dehydrogenase kinase complex (BCKDK), which encodes the key regulatory enzymes in branched-chain amino acid (BCAA) catabolism, in two breeds of horses, Thoroughbred and Jeju, at different time intervals. The concentrations of metabolites in muscle and plasma were measured by ¹H NMR (nuclear magnetic resonance) spectroscopy, and the relative metabolite levels before and after exercise in the two samples were compared. Subsequently, multivariate data analysis based on the metabolic profiles was performed using orthogonal partial least square discriminant analysis (OPLS-DA), and variable important plots and t-test were used for basic statistical analysis. The stress-induced expression patterns of BCKDK genes in horse muscle-derived cells were examined using quantitative reverse transcription polymerase chain reaction (qPCR) to gain insight into the role of transcript in response to exercise stress. In this study, we found higher concentrations of aspartate, leucine, isoleucine, and lysine in the skeletal muscle of Jeju horses than in Thoroughbred horses. In plasma, compared with Jeju horses, Thoroughbred horses had higher levels of alanine and methionine before exercise; whereas post-exercise, lysine levels were increased. Gene expression analysis revealed a decreased expression level of BCKDK in the post-exercise period in Thoroughbred horses.

• Journal of Korean Society of Forest Science
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• v.46 no.1
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• pp.10-20
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• 1980

This experiment was carried out to investigate the effects of physico-chemical properties of soil on the growth of Chamaecyparis obtusa and to apply the results to such rational forest management as yield forecast and selection of suitable species for a given forest stand. The soil properties observed in this experiment were soil pH, exchangeable pH organic matter, total nitrogen, available $P_2O_5$, cation exchange capacity, exchangeable $H^+$, total base and base saturation. Diameter at breast height (DBH), height and volume growth of C. obtusa were observed at 78 sampling sites. Data were processed into the following series for the analysis of multivariates : inner correlation matrix, multiple correlation coefficients, regression coefficients and partial correlation coefficients. The results are summerized as follows : 1. Multiple correlation coefficients between soil properties and DBH of C. obtusa were .364 for 20-year trees, resulting less efficient for estimating the growth of C. obtusa. However, total base, soil pH and base saturation were considerable characters for better estimation. 2. More efficient multiple correlation coefficients were observed between soil properties and height growth than those between soil properties and DBH, showing .443 for 20-year trees and factors affecting the height growth were similar to those of DBH. 3. Extremely low values of multiple correlation coefficients were estimated between physico-chemical properties of soil and volume growth of C. obtusa resulting low efficient estimation. However, total base contributed highly to volume growth of C. obtusa. Accordingly the most contributable factor for estimating the growth of C. obtusa were total base, soil pH and base saturation. Consequently, these results can be effective for selecting of the reforesting site, and less effective for estimating the growth of C. obtusa.

• Clinics in Shoulder and Elbow
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• v.21 no.2
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• pp.67-74
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• 2018

Background: Fatty degeneration of rotator cuff is a well-known predictor of postoperative outcome. The purpose of this study was to evaluate the clinical features of rotator cuff tears involving subscapularis, and investigate whether fatty degeneration quantified from only the upper subscapularis correlates better with clinical outcomes than quantified from the whole subscapularis. Methods: We retrospectively analyzed 315 consecutive patients who underwent arthroscopic repair for rotator cuff tears involving subscapularis with a minimum follow-up of 1 year. Preoperative and postoperative visual analogue score for pain, range of motion and functional scores were assessed. Integrity of the repaired tendon was assessed at the 1-year follow-up with either magnetic resonance imaging or ultrasonography. Results: The mean Goutallier grade of whole cross-section was significantly lower than that of upper cross-section (1.59 vs. 1.71, p<0.05), but significantly higher than that of lower cross-section (1.59 vs. 1.01, p<0.05). In analysis of 37 re-tears, the occupancy of severe fatty degeneration in upper cross-section was 86.5%, which was significantly higher than that seen in whole cross-section (56.8%, p<0.05). We calculated the cut-off tear size for prediction of re-tears as 19.0 mm for retraction and 11.0 mm for superior-inferior. The cut-off Goutallier grade was 2.5 for both whole and upper cross-sections, but area under the curve was greater in the upper cross-section than the whole (0.911 vs. 0.807). Conclusions: As fatty degeneration of upper subscapularis demonstrated a more distinct spectrum than whole subscapularis, we suggest that measuring fatty degeneration of upper subscapularis can be a more useful method to predict clinical prognosis.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.24 no.2
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• pp.31-47
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• 2013

The purpose of this study is to develop a measurement tool for self-image of the teacher librarian by semantic differential meaning scale and to analyse the correlation between their self-image and individual characteristics. This study suggests that the teacher librarians have regarded themselves as friendly, planned, sensitive and cooperative persons as well as persons with discernment. On the other hand, there are some negative self-images such as partial, poor and uninfluential persons plus disregarder and a reserved persons. The educational career of teacher librarians' background has influence on evaluation area of the self-image. This result shows that senior teacher librarians' role performance as a adviser or a leader is very important. So mediator role of KLA and KSLA has to be reinforced to beat the exchange and collaboration between the senior and the junior teacher librarians. It is necessary to appoint the teacher librarians obligatorily in oder to feel their professional security and sense of achievement, also to appreciate expertise of them through role clarification among the human resources of the school library.

• Journal of Microbiology and Biotechnology
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• v.22 no.11
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• pp.1523-1531
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• 2012

The metabolite profile of meju during fermentation was analyzed using mass spectrometry techniques, including GC-MS and LC-MS, and the bacterial diversity was characterized. The relative proportions of bacterial strains indicated that lactic acid bacteria, such as Enterococcus faecium and Leuconostoc lactis, were the dominant species. In partial least-squares discriminate analysis (PLS-DA), the componential changes, which depended on fermentation, proceeded gradually in both the GC-MS and LC-MS data sets. During fermentation, lactic acid, amino acids, monosaccharides, sugar alcohols, and isoflavonoid aglycones (daidzein and genistein) increased, whereas citric acid, glucosides, and disaccharides decreased. MS-based metabolite profiling and bacterial diversity characterization of meju demonstrated the changes in metabolites according to the fermentation period and provided a better understanding of the correlation between metabolites and bacterial diversity.

• Journal of Korean Neurosurgical Society
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• v.41 no.5
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• pp.283-290
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• 2007

Objective : This study was designed to analyze seizure outcome and to investigate the prognostic factors for predicting seizure outcome according to the preoperative evaluations, surgical procedures, topectomy sites and histopathological findings in patients with extratemporal lobe epilepsy [ETLE]. Methods : This study comprised 63 patients with ETLE who underwent surgery. Preoperative evaluations included semiologic analysis, chronic video-EEG monitoring, and neuroimaging studies. Surgical procedures consisted of topectomy in 51 patients, corpus callosotomy in 9, functional hemispherectomy in 2, and vagus nerve stimulation [VNS] in 1. Histopathological findings were reviewed. Postoperative seizure outcomes were assessed by Engel's classification at the average follow up period of 66.8 months. Chi-square test was used for statistics. Results : Total postoperative seizure outcomes were class I in 51 [80%] patients, class II in 6 [10%], class III in 6 [10%]. Patients with structural abnormalities on neuroimaging study showed class I in 49 [88%] patients [p<0.05]. Patients with focal and regional ictal EEG onset revealed class I in 47 [90%] patients [p<0.05]. Semiologic findings, surgical procedures, topectomy sites and histopathological findings did not show statistical correlation with seizure outcome [p<0.05]. Conclusion : A good seizure outcome was obtained in patients with ETLE. The factors for favorable seizure outcome are related to the presence of structural abnormalities on neuroimaging study, and focal and regional ictal EEG onset.

• The Journal of the Korea Contents Association
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• v.20 no.9
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• pp.558-576
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• 2020

The purpose of the research was to verify the mediating effect of social support and cognitive emotional regulation strategy when office worker's emotional expressive ambivalence influences on interpersonal stress. A survey was conducted with 422 office workers from August to September of 2018. SPSS, AMOS and Mplus were used for descriptive statistics, correlation analysis, verification of structural equation modeling and mediated effect. There was a partial mediating effect in emotional expressive ambivalence of self-defense model, a complete mediating effect in relational emotional expressive ambivalence model. In both self-defense and relational emotional expressive ambivalence model, double mediation effect of passing through support and cognitive emotional regulation strategy in the relationship between emotional expressive ambivalence and interpersonal stress was significant. This study sought ways to reduce interpersonal stress by increasing the level of perception of social support and using adaptive cognitive emotional control strategies for office workers.

• Journal of Life Science
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• v.8 no.5
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• pp.492-496
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• 1998

Intact measurement of leaf water content was attempted using the transmission of beta radiation. Prior to the experiments, two tested plants, rice and soybean, were grown in 1/5000a wagner pot. The moisure ratio of plant leaves were measured with the beta radiation transmission method using a G-M detector and $^{99}$ Tc as the beta ray sou-rce. Beta radiation transmission showed a tendancy to increase with the passage of time after leaf cutting. However, it showed a tendancy to decrease with water supply for the lack of water. A positive correlation was found between the leaf water content and beta radiation transmission. The mutiple regression analysis about leaf water content was obtained that the coefficient of partial regression for beta radiation transmission was much higher(rice; -0.863, soybean; -0.904) than that for specific leaf area(rice; 0.007, soybean: 0.004).

• Journal of the Korean Chemical Society
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• v.51 no.1
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• pp.21-30
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• 2007

A multiwavelength spectrophotometric titration method was applied to study protolytic constants of four water-soluble vitamins, folic acid(vitamin B9 or B0), thiamine(vitamin B1), riboflavin(vitamin B2) and pyridoxal (vitamin B6) in binary ethanol-water mixtures at 25oC and an ionic strength of 0.1M NaNO3. The protolytic equilibrium constants, spectral profiles, concentration diagrams and also the number of components has been calculated from the curve fitting of the pH-absorbance data with appropriate mass balance equations by an established factor analysis model. DATAN program was used for determination of acidity constant and SPECFIT program was used for calculation of standard deviations and partial correlation coefficients. A glass electrode calibration procedure based on the four parameter equation pH=α+SpcH+JH+[H+]+ JOH-Kw/[H+] based on the Gran,s plots was used to obtain pH-readings in the concentration scale (pcH). The effect of the solvent on the protolytic constants was discussed.

• The Journal of Engineering Geology
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• v.17 no.2 s.52
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• pp.263-270
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• 2007

Tunnel behavior measured by monitoring during construction is a main item for safety evaluation and it depends widely upon local geological characteristics. To assess in this study how much the geological factors influence on tunnel behavior for each RMR factor, a quantification analysis was carried out using tunnel face maps and measurements as explanatory variables and dependent variables, respectively. The results showed that average significance of the influence of RMR factors - R1, R2, R3, R4 and R5, on tunnel displacements are 17.0%, 20.4%, 20.4%, 11.6% and 30.6%, respectively, and this probably indicates that the groundwater condition played a significant role for the tunnel displacement.