• Applied Biological Chemistry
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• v.46 no.4
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• pp.385-390
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• 2003

An antimicrobial peptide was purified from the roots of Phytolacca americana L. and was designated as PAMP-r. Purification was carried out by DEAE-cellulose anion exchange, sephadex G-75 gel filtration, Mono S cation exchange, and Resource RPC reverse phage chromatography. The molecular weight of PAMP-r was estimated to be about 4,900 Da by 15% SDS-PAGE under reducing condition. PAMP-r exhibited a broad spectrum of antimicrobial activity. PAMP-r was stable against heat and pH treatment; its activity was not diminished by the heat treatment up to $80^{\circ}C$ for 30 min, and it showed a pH stability in the range between pH 3.0 to pH 8.0.

• Microbiology and Biotechnology Letters
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• v.29 no.4
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• pp.248-252
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• 2001

Recombinant Human serum albumin (rHSA) was purified to near homogeneity from H, polymorpha using heat treatment, ultrafiltratipn and Phenyl Sepharose CL-4B and Mono Q column chro - matographies with a recovery yield of 60% The molecular weight of the purified rHSA was estimated to be about 65,000 Da by denaturing SDS-PAGE The N terminal amino acid sequence of the purified HSA determined by Edman degradation was turned out to be Asp- Ala- His- Lys- Ser- Glu- Ala, suggesting that the rHSA expressed in H, polymorpha was efficiently secreted and correctly processed at the cleavage site of secretion signal sequence. The purified human albumin showed the pI value identical to that of authentic human serum albumin.

• Korean Journal of Animal Reproduction
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• v.14 no.2
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• pp.125-131
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• 1990

These experiments were carried out to investigate of the molecular characteristics of porcine zona pellucidae and to examine the reactivity of anti-zona antibody by SDS-PAGE, Immunoblotting and Immunoprecipitation. The results obtained in these experiments were summarized as follows : 1. The result obtained by SDS-PAGE of porcine zona pellucidae indicated that it composed of several units with molecular weight ranging 55,000-110,000. 2. In order to see the reactivity of antibodies to zona pellucidas, immunoblotting was applied. The results indicated that polyclonal antibodies to porcine and mouse zona reacted with porcine zona. While monoclonal antibody to porcine did not react with the procine zona enough to show a clear band on a gel. 3. Labelling of porcine zonae with 125I was performed using the Iodogen method, the radioactivity and the percent incorporation of 125I into porcine zonae were approximately 26,000 cpm/10${mu}ell$ and 16, respectively. 4. Measurements of radioactivity and O.D value for Immunoprecipitates produced by reaction of 125I-porcine zona with anti-zona antisera were confirmed that existence of reactivity of monoclonal antibody to porcine zona although its reactivity was lower than that of polyclonal antibodies, and reconfirmed that cross-reactivity of polyclonal antibody of mouse zona with porcine zona.

• Food Science and Biotechnology
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• v.15 no.1
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• pp.128-132
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• 2006

This study was undertaken to investigate the effect of peptic and chymotryptic hydrolyses of 7S globulin, the major allergen of soybean protein, on its allergenicity, as measured by enzyme linked immunosorbent assay (ELISA), and to identify the allergenic hydrolyzed fragments of 7S globulin using SDS-PAGE. When 7S globulin was hydrolyzed by pepsin, the allergenicity was reduced by over 50%. However, the allergenicity of 7S globulin reduced by peptic hydrolysis was recovered in the sera from 5 out of 10 patients following sequential chymotryptic hydrolysis. Two fragments, with molecular weights 20-25 and 13-16 kDa, among the hydrolysate of 7S globulin by sequential pepsin and chymotrypsin showed reactivity with sera from 10 soybean-allergenic patients. As a result of the theoretical hydrolyses of ${\beta}$-conglycinin, which is a major protein of 7S globulin, it is suggested that the 20-25 kDa fragments were the fragments of the ${\alpha}$-subunit of ${\beta}$'-conglycinin and that the 10-16 kDa fragments were from the ${\alpha}$'-subunit.

• Journal of Life Science
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• v.12 no.2
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• pp.151-157
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• 2002

A trypsin inhibitor was isolated and purified from Azismatis Rhizoma which has been used as a galenic for diuretic and antiphlogistic. Purification was carried out by 0-80% saturated ammonium sulfate salting out, DEAE- cellulose ion exchange chromatogrphy, Sephadex G-150 gel filtration. The molecular weight of Alismatis Rhizoma trypsin inhibitor(ARTI) was estimated to be about 23,000 Da by gel filtration and SDS-PAGE, it must be monomer. ARTI was stable at 0~6$0^{\circ}C$, but at higher temperature its activity was decreased about 35%. When benzoyl-dl-arginine p-nitroanilide was used as a substrate of trypsin, half-maximal inhibition of ARTI was observed at 0.071 $\mu$M. ARTI inhibited the hydrolysis of trypsin non-competitively and Km value was 0.81 $\mu$M.

• Journal of Digital Contents Society
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• v.14 no.1
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• pp.15-23
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• 2013

In response to the demands of large-scale data processing with low-power and new application, a storage system using SSD (Solid State Disk/Drive) with fast input-output performance instead of hard disc has appeared as storage device. Studies on methods to overcome specific problems of SSD such as various processing data units, out-place-update and limited delete count have been actively conducted. However, declining performance and stability have not been resolved yet when storing case specific data with small scale that causes frequent random write in hard disc or SSD. This thesis suggests a system structure that stores index requesting frequent random write in NVRAM capable of byte access by using characteristics such as byte unit fast read / write of NVRAM, non-volatile and smaller size of actual changed data size in index page than block size.

• 한국HCI학회:학술대회논문집
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• 2008.02a
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• pp.648-653
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• 2008

With development of mobile communications terminal and expansion of mobile Internet, a large number of users can use mobile web contents at anytime and anywhere and will demand service of greater contents. Due to such demand, many studies are being carried out on transcoding contents so that wired web contents can be used in mobile web. However, transcoding and creation of mobile web contents involve difficulties because specifications available from telecommunications companies and mobile terminals have not been standardized. Especially, in order for serving dynamic object of wired web page contents to dynamically change according to time or user, it is required not only to program scripts to suit each terminal, but also to transcode the resources used in advance. For solution to this problem, this study uses the hierarchy structure of DOM (document object model) to display structural characteristics of wired web page. In other words, this study proposes the following technique Wired web pages are analyzed and the results are established as data structure. Then, dynamic object is extracted and the domain is indexed so that, when serving mobile web page, information can be extracted at the indexed position to create mobile web contents for service on real-time basis.

• Journal of Korean Library and Information Science Society
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• v.24
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• pp.413-448
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• 1996

Nowadays, the World Wide Web (WWW) has become an important resource of timely information for the information-related people such as information scientists, librarians, and students in Library and Information Sciences area. They are information professionals who navigate the information on the internet. Also, they need to be information providers who build a WWW homepage. This paper is a study of the necessity, preparation, and building WWW Homepage files for school of Library and Information Sciences in the age of competition among disciplines. It is particularly focused on the colleges and universities in Republic of Korea (ROK) and North America area. The purpose of this study is to provide various kinds of reference information to prepare a homepage in the future as utilizing information on the internet effectively. Even though a Web page was necessary for a school of Library and Information Sciences to show news, and introduce the purpose of the disciplines and the curriculum of the school, and the professors of a class etc., it was not well-prepared yet in ROK. However, a web page was used well enough and prosperous in North America area (Canada, the United States, and Puerto Rico) comparatively. Those web pages were analyzed and studied to prepare for a good designing of homepages for school of Library and Information Sciences in Korea and for the age of competition among disciplines in this paper. Suggestions for designing a good homepage and guidelines for preparing a best one were studied after both reviewing literature and utilizing experiences by the author who currently serves in the School of Library and Information Sciences in Keimyung University and builds homepage for the school. As a result, the major suggestions are ; premiered, and they are as follows: (1) English version of a homepage is necessary, (2) Provide a multimedia presentation about the nature of a school (3) Incorporate a place to let people make suggestions on the contents (of a homepage), (4) Bear in mind that potential users must be familiar with abbreviations you used, (5) Absolutely do not use abbreviations that may make the content more difficult to understand, (6) Add a feature on the every single page that will take the user back to the main page, (7) Use clear, short and well-structured sentences and remember to divide text into paragraphs, (8) Date with a creation or modification date in the homepage to indicate the updated date, (9) Being a multimedia environment, use colors effectively (the guidelines were also suggested), and (10) Put colleges' name into the title of bookmarks to find out them easily.

• The KIPS Transactions:PartD
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• v.13D no.4 s.107
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• pp.463-476
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• 2006

R-trees have been traditionally optimized for the I/O performance with the disk page as the tree node. Recently, researchers have proposed cache-conscious variations of R-trees optimized for the CPU cache performance in main memory environments, where the node size is several cache lines wide and more entries are packed in a node by compressing MBR keys. However, because there is a big difference between the node sizes of two types of R-trees, disk-optimized R-trees show poor cache performance while cache-optimized R-trees exhibit poor disk performance. In this paper, we propose a cache and disk optimized R-tree, called the PR-tree (Prefetching R-tree). For the cache performance, the node size of the PR-tree is wider than a cache line, and the prefetch instruction is used to reduce the number of cache misses. For the I/O performance, the nodes of the PR-tree are fitted into one disk page. We represent the detailed analysis of cache misses for range queries, and enumerate all the reasonable in-page leaf and nonleaf node sizes, and heights of in-page trees to figure out tree parameters for best cache and I/O performance. The PR-tree that we propose achieves better cache performance than the disk-optimized R-tree: a factor of 3.5-15.1 improvement for one-by-one insertions, 6.5-15.1 improvement for deletions, 1.3-1.9 improvement for range queries, and 2.7-9.7 improvement for k-nearest neighbor queries. All experimental results do not show notable declines of the I/O performance.

• Parasites, Hosts and Diseases
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• v.34 no.1
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• pp.49-58
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• 1996

Characterization of a purified proteinase from T4chomoncs uoginalis was carried out using bacitracin-sepharose affinity chromatography. Trichomonos uqginolis KT-9 isolate was used as a source of eye study Proteinase activity was determined using Bz-Pro- Phe-Arg-Nan as the substrate. Optimum pH for the purified proteinase activity was 7.0 and 6.0, 9.0 with DTT. Optimum temperature was 37℃ and isoelectric point was 7.2 Activity of this proteinase was inhibited by E-64, antipain, leupeptin, Hg2+ and Zn2+ and activated by DTT and cysteine. Activity of the purified proteinase was visualized by gelatin SDS- PAGE. The gelatinolytic activity of the purified proteinase was inhibited by E-64, antipain, leupeptin, and IAA, but not by PMSF and EDTA. On SDS-PAGE, the molecular weight of the purified proteinase was 60,000 daltons. Sera of rabbits infected with T. vaginalis reacted specifically in immunoblots with this proteinase. These results indicate that 60 kDa of purified proteinase was cysteine proteinase with antigenicity.