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Effect of Cooking and Processing on the Phytate Content and Protein Digestibility of Soybean (대두의 조리 가공에 따른 Phytate 함량 및 단백질 소화율)

  • Kim, Hee-Seung;Yoon, Jae-Young;Lee, Su-Rae
    • Korean Journal of Food Science and Technology
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    • v.26 no.5
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    • pp.603-608
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    • 1994
  • This study was undertaken to find out the effect of phytate on the protein digestibility of various soybean foods, including soy milk, bean curd, curd residue, cheongkukjang, soy sauce, and soy paste. The phytate content of soybean was 2.4%, which decreased to 0.2%, 0.7%, and 0.4% in soy milk, bean curd, and curd residue, respectively, and to 0.2% and 1.0% in soy sauce and soy paste, respectively. The phytate/protein ratio was not correlated with protein digestibility by pepsin whereas the ratio was highly correlated with pancreatin digestibility (p<0.01, r= -0.73). According to SDS-PAGE for the soluble protein fractions, soaked bean showed an alteration in soluble components and bean curd residue exihibited newer low molecular weight bands. Fermented soy products showed no protein band, likely due to degradation.

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An Optimal Way to Index Searching of Duality-Based Time-Series Subsequence Matching (이원성 기반 시계열 서브시퀀스 매칭의 인덱스 검색을 위한 최적의 기법)

  • Kim, Sang-Wook;Park, Dae-Hyun;Lee, Heon-Gil
    • The KIPS Transactions:PartD
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    • v.11D no.5
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    • pp.1003-1010
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    • 2004
  • In this paper, we address efficient processing of subsequence matching in time-series databases. We first point out the performance problems occurring in the index searching of a prior method for subsequence matching. Then, we propose a new method that resolves these problems. Our method starts with viewing the index searching of subsequence matching from a new angle, thereby regarding it as a kind of a spatial-join called a window-join. For speeding up the window-join, our method builds an R*-tree in main memory for f query sequence at starting of sub-sequence matching. Our method also includes a novel algorithm for joining effectively one R*-tree in disk, which is for data sequences, and another R*-tree in main memory, which is for a query sequence. This algorithm accesses each R*-tree page built on data sequences exactly cure without incurring any index-level false alarms. Therefore, in terms of the number of disk accesses, the proposed algorithm proves to be optimal. Also, performance evaluation through extensive experiments shows the superiority of our method quantitatively.

Form I Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase from CO2-Fixing Chemoautotrophic bacterium, Aeromonas sp. strain JS-1: Purification and Properties (CO2를 고정하는 화학독립영양미생물인 Aeromonas sp. strain JS-1의 Form I Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase 정제 및 특성 파악)

  • Na, Suk-Hyun;Bae, Sang-Ok;Jung, Soo-Jung;Chung, Seon-Yong
    • KSBB Journal
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    • v.25 no.6
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    • pp.559-564
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    • 2010
  • A new hydrogen-oxidizing bacterium, Aeromonas sp. strain JS-1, that can fix $CO_2$ via the reductive pentose phosphate cycle (Calvin-Benson cycle) under chemoautotrophic conditions but not photoautotrophic conditions was isolated from fresh water. Strain JS-1 showed considerable $CO_2$ fixation ability during continuous cultivation even at high $CO_2$ concentration. Strain JS-1 used $H_2$ and $CO_2$ fixation as energy and carbon sources, respectively. Carbon dioxide fixation is carried out through the Calvin-Benson cycle, in which ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) is the key enzyme. Hydrogen-oxidizing chemoautotrophic Aeromonas sp. strain JS-1 exhibited remarkedly strong RubisCO [EC 4.1.1.39] activity. RubisCO was purified as an $L_8S_8$-type hexadecamer with molecular mass of 560 kDa by gel filtration. The enzyme consisted of two different subunits eight large (56 kDa) and eight small (15 kDa), as demonstrated by SDS-PAGE. The specific activity of the purified enzyme was about 3.31 unit/mg and stable up to $45^{\circ}C$. The $K_m$ values for RuBP, $CO_2$, and $Mg^{2+}$ were estimated to be 0.25 mM, 5.2 mM and 0.91 mM, respectively.

Towards Establishing a Touchless Gesture Dictionary based on User Participatory Design

  • Song, Hae-Won;Kim, Huhn
    • Journal of the Ergonomics Society of Korea
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    • v.31 no.4
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    • pp.515-523
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    • 2012
  • Objective: The aim of this study is to investigate users' intuitive stereotypes on non-touch gestures and establish the gesture dictionary that can be applied to gesture-based interaction designs. Background: Recently, the interaction based on non-touch gestures is emerging as an alternative for natural interactions between human and systems. However, in order for non-touch gestures to become a universe interaction method, the studies on what kinds of gestures are intuitive and effective should be prerequisite. Method: In this study, as applicable domains of non-touch gestures, four devices(i.e. TV, Audio, Computer, Car Navigation) and sixteen basic operations(i.e. power on/off, previous/next page, volume up/down, list up/down, zoom in/out, play, cancel, delete, search, mute, save) were drawn from both focus group interview and survey. Then, a user participatory design was performed. The participants were requested to design three gestures suitable to each operation in the devices, and they evaluated intuitiveness, memorability, convenience, and satisfaction of their derived gestures. Through the participatory design, agreement scores, frequencies and planning times of each distinguished gesture were measured. Results: The derived gestures were not different in terms of four devices. However, diverse but common gestures were derived in terms of kinds of operations. In special, manipulative gestures were suitable for all kinds of operations. On the contrary, semantic or descriptive gestures were proper to one-shot operations like power on/off, play, cancel or search. Conclusion: The touchless gesture dictionary was established by mapping intuitive and valuable gestures onto each operation. Application: The dictionary can be applied to interaction designs based on non-touch gestures. Moreover, it will be used as a basic reference for standardizing non-touch gestures.

Relevance of the Cyclomatic Complexity Threshold for the Web Programming (웹 프로그래밍을 위한 복잡도 한계값의 적정성)

  • Kim, Jee-Hyun
    • Journal of the Korea Society of Computer and Information
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    • v.17 no.6
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    • pp.153-161
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    • 2012
  • In this empirical study at the Web environment based on the frequency distribution of the cyclomatic complexity number of the application, the relevance of the threshold has been analyzed with the next two assumptions. The upper bound established by McCabe in the procedural programming equals 10 and the upper bound established by Lopez in the Java programming equals 5. Which numerical value can be adapted to Web application contexts? In order to answer this 10 web site projects have been collected and a sample of more than 4,000 ASP files has been measured. After analyzing the frequency distribution of the cyclomatic complexity of the Web application, experiment result is that more than 90% of Web application have a complexity less than 50 and also 50 is proposed as threshold of Web application. Web application has the complex architecture with Server, Client and HTML, and the HTML side has the high complexity 35~40. The reason of high complexity is that HTML program is usually made of menu type for home page or site map, and the relevance of that has been explained. In the near future we need to find out if there exist some hidden properties of the Web application architecture related to complexity.

Web-based Distributed Experimental Frame for Discrete Event Simulation System (이산사건 시뮬레이션 시스템을 위한 웹 기반 분산 실험 틀)

  • Jung, Inho;Choi, Jaewoong;Choi, Changbeom
    • Journal of the Korea Society for Simulation
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    • v.26 no.2
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    • pp.9-17
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    • 2017
  • The problem of social phenomenon is getting more complicated than past decades, and the simulation engineers need more computation power to solve the problem. Therefore, the needs of the computational resources of the modeling and simulation environment are increasing. In the perspective of the simulation, it is necessary to allocate computational resources flexibly so that the simulation can be performed per the available budget range. As an alternative to the simulation environment to accommodate these requirements, cloud service has emerged as an environment in which computing resources can be used flexibly. This paper proposes a web-based simulation framework which consists of a front-end that reconstructs the simulation model using the web, and a back-end that executes the discrete event simulation. This paper also carried out a case study which shows web-based simulation framework has better overall runtime than standalone simulation framework.

College Students' Preferences of Web-based OPAC Retrieval Techniques and their Blood Types: An Empirical Study (대학생들의 웹 기반 OPAC 검색기법 선호도와 혈액형에 대한 실험적 연구)

  • Kim, Hee-Sop
    • Journal of the Korean Society for Library and Information Science
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    • v.44 no.3
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    • pp.81-102
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    • 2010
  • The purpose of this study was to investigate college students' preferences of Web-based OPAC retrieval techniques and their ABO blood types as an empirical survey. Data was collected through a self-designed questionnaire with a total of 101 undergraduate students from the College of Social Sciences responding. The collected data was analyzed using descriptive statistics, and One-way ANOVA. The results show that 'title' was most preferred among the access points, 'AND' was the most preferred Boolean operator, 'publication year' and 'subject' were the most favored techniques in limiting the scope of retrieval, and 'record number limit per page' was the most frequently used for displaying retrieval results. The results also show that there were little(3 out of 22, i.e. 13.6%) statistically significant differences between the college students' preferences of Web-based OPAC techniques and their blood type.

An Evaluation of Biotope Based on Its Valuation Criteria in Terms of Conservation of Species and Habitat (평가지표에 의한 도시 비오톱의 가치평가 -생물종과 서식처 보전을 중심으로-)

  • 나정화;류연수;사공정희
    • Journal of the Korean Institute of Landscape Architecture
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    • v.29 no.1
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    • pp.100-112
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    • 2001
  • The purpose of this study is to construct the evaluation criteria based on the relative importance and various combination of each evaluation index and then to evaluate urban biotope in terms of conservation of species and habitat. After the standard of evaluation had been developed through the literature review, the relative importance of the evaluation index through expert survey was found out, and the evaluation model of biotope value has been established. The results of this study are as follows; 1) We have constructed the nineteen evaluation criteria through literature reviews, and have provided relative importance to each evaluation index of the diversity of typical species in biotope, afforestation rate, stratification, Hemeroby grads, and the diversity of landscape patterns based on the results of expert survey. and then we have constructed the evaluation system using the relative importance criteria. 2) To evluate urban biotope using the evaluation system constructed, we selected 14 sites. The final evaluation results of each site is as follows; The III grade areas are Seohan and Hwasung high-rise apartment at Beummul-Dong, Korea Men Power /Agency in Sungs 대 industrial zone, the adjacent area of St. Tongil, and Hyomyung elementary school. The II grade areas are the farm land behind the third Anshim Jugong apartment, and Ehyun park. The I grade areas are swampy land in front of Kangchon and Anshim construction site, water space with semi-natural style in front of Gangchon Woobang apartment, forest zone near page Temple at mt. Palgong, the edge area of Jisan and Gosan, and the tendril yard against Anshim middle school. The only grade IV area is the commercial zone near Taegu Department 3) It is highly useful to evaluate urban biotope using the evaluation criteria constructed for this research, the evaluation criteria we developed may be difficult to guarantee the ability for reproduction and recovery, the appearance-number of the rare and endangered species are difficult to estimate urban biotopes. In the future, it is highly useful to evaluate biotope in terms of conservation of species and habitat through its valuation criteria developed in this research and it is necessary to develop the evaluation criteria applicable to estimation of biotope inventory and reproduction of biotope.

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Purification, Bacteriolytic Action and Plasmid Isolation of Acidocin 4A Produced by Lactobacillus acidophilus GP4A (Lactobacillus acidophilus GP4A 박테리오신의 정제, Bacteriolytic 작용 및 생산 관련 Plasmid의 선별)

  • Han, Gyeong-Sik;Jeon, U-Min;Kim, Yeong-Hun;Kim, Se-Heon
    • Journal of Dairy Science and Biotechnology
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    • v.21 no.2
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    • pp.114-119
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    • 2003
  • Acidocin 4A produced by Lactobacillus acidophilus GP4A was purified to homogeneity by ammonium sulfate precipitation and sequential chromatographies containing Octyl sepharose CL-4B column, $C_{18}$ Sep-Pak Cartridge, $C_{18}$ RP HPLC and HPLC gel filtration. Tricine SDS-PACE resulted in a single band with estimated molecular mass of 4.1 kDa corresponding to the polypeptide weight marker. Electron microscopy of acidocin-treated indicator cells(L. delbrueckii subsp. lactis ATCC 4797) confirmed that acidocin 4A presented bacteriolytic effect, resulting in cell lysis. Curing trial using ethidium bromide (EtBr) was carried out to examine whether acidocin 4A determinant was encoded either by chromosome or on plasmid. The plasmid designated as pLA4A, being about 20 kb in size, was responsible for acidocin 4A production and immunity to host cells.

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Salmonella enteritidis의 편모항원에 대한 난황항체의 ,생산 x Production of Egg Yolk Antibodies against Flagella Antigen of Salmonella enteritidis

  • 김정우
    • Korean Journal of Poultry Science
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    • v.25 no.4
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    • pp.161-167
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    • 1998
  • This experiment was carried out to develope the production of specific yolk antibody from laying hens immunized with antigens from Salmonella enteritidis. Antigenic protein isolated from the flagella of Salmonella enteritidis, determined by SDS-PAGE, was pure and has a molecular mass of approximately 54.6 kDa. It was observed that the antibody titers both in egg yolk and serum were performed at 2 weeks after immunization with flagella antigen to the laying hen. And the level was increased gradually to 6 weeks after immunization. At the time of 6 weeks, the antibody titer of yolk showed higher than that of serum. According to the results of specificity test(ELISA), the yolk antibody did not react with different bacterial strains(S. choleraesuis, ETEC Kl2:K99, K88,987P), but reacted only with S. enteritidis strain. The contents of immunoglobulin(IgY) in an egg yolk was 106mg approximately. By the isolation procedure of IgY from the egg yolk, 88.3 percent of IgY content was recovered in this study.

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