• Journal of Pharmacopuncture
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• v.4 no.2
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• pp.5-15
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• 2001

This study was carried out for production of neutral antibody to bee venom $(anti-phospholipase\;A_2IgY)$. Hen layings were injected repeatedly with bee venom and phospholipase $A_2$ with Freund's adjuvant. Specific antibody in egg yolk from immunized hen laying was separated, and purified, also immunological characteristics of anti phospholipase $A_2\;IgY$ was invested. The results were summarized as follows: 1. Phospholipase $A_2$ was showed single band at molecular weight 17,000 in SDS-PAGE and bee venom was showed two band at molecular weight 17,000 and under molecular weight 6,500 in SDS-PAGE. 2. During 70 days after hen immunized with bee venom and phospholipase $A_2$, antibodies(anti-bee venom IgY) to bee venom were showed poor ELISA value in egg yolk, but antibodies$(anti-Phospholipase\;A_2IgY)$ to phospholipase $A_2$ in egg yolk were increased ELISA value from 8 days or 15 days and found maximum ELISA value at 42 days. Also after booster at 49 days, ELISA value of anti Phospholipase $A_2\;IgY$ in egg yolk was supported at optical density(O.D) 1.0 level, continuously. 3. Titer of phospholipase $A_2\;IgY$ was showed 1: 32,000. 4. In double immunodiffusion test to phospholipase $A_2$ after double dilution of anti-phospholipase $A_2\;IgY$, only precipitation line was made in 1:1 dilution well of anti-Phospholipase $A_2\;IgY$. But In immunodiffusion test to anti-phospholipase $A_2\;IgY$ after double dilution of phospholipase $A_2$, Precipitation line to 250ul/ml well of phospholipase $A_2$ was showed. In double immunodiffusion test to bee venom(1mg/ml) after double dilution anti-phospholipase $A_2\;IgY$, all well without 1:32 dilution well were showed strong precipitation line. 5. In dot bloting test to anti-phospholipase $A_2\;IgY$ after diluting bee venom(0.5mg/ml), dot bloting color was showed clearly to $1/100(5{\mu}g/ml)$ in bee venom.

• Journal of Life Science
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• v.23 no.2
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• pp.259-266
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• 2013

To isolate the fibrinolytic enzyme, 268 strains from 21 samples were morphologically isolated from Cheonggukjang collected from Korea and Japan. Among the 268 strains, protease-producing bacteria were isolated in nutrient agar medium including 1% skimmed milk. As a result of this, 22 strains were isolated. Apiweb site was used to identify these strains based on their biochemical properties. In addition, 16S rRNA sequencing was performed to identify the strain. Most of the identified strains were Bacillus subtilis and B. amyloliquefaciens. Fibrinolytic enzyme activity was measured with the fibrin plate method. Five strains were finally selected: A2-14, A2-20, C1-05, C1-09, and F2-01. Of those five strains, the A2-20 strain, which is close to B. amyloliquefaciens, showed the strongest fibrinolytic activity. The fibrinolytic enzyme produced by the A2-20 strain was partially purified from culture supernatant by gel filtration and ion exchange chromatography. The optimal pH and temperature values of the partially purified enzyme were 7.0 and $35^{\circ}C$, respectively. Purified protein analysis was carried out with SDS-PAGE and zymography. A genetic analysis was also conducted by PCR based on the consensus sequence of fibrinolytic enzyme. Corresponding genes with a partial sequence of the A2-20 strain were identified.

• Journal of Internet Computing and Services
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• v.7 no.2
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• pp.37-52
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• 2006

A mobile device like ceil phone is the necessity of modern people, of ich con be easily connected to a wireless internet through such a mobile device. The demand for a wireless data communication is growing rapidly. However, agencies have not yet completed standardization of a markup language. Due to the development of the Mobile Device, agencies in this field have provided different data formats with each Mobile Device Platform. Traditionally, contents is hand-tailored to suit the target device. A key problem is that the characteristics and capabilities of the mobile device are too diverse to service the most suitable mobile contents. Because of these problems, the need for a re-usable document description language increases. In this paper, we defined Template file that is common data to service mobile devices. We proposed a method that could be an effective wireless web service though design and the implementation of the Call manager & the XSL Generator. In the methodology, when requesting a wireless internet service, a mobile device finds out markup language and a hardware specification of the mobile device through the Call Manager component supports. The XSL Generator component creates the XSL file dynamically that is the most suitable to a device. Finally, contents is serviced to each device by XSLT. It can generate a wireless page more easily by reusing the existing web contents through such course. Therefore, it can save the time and expense for generating a wireless page.

• Korean Chemical Engineering Research
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• v.60 no.3
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• pp.386-391
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• 2022

This study is about a technique for obtaining collagen by extracting fat by treating collagen-containing liposuction effluent in the presence of supercritical fluid. Using a supercritical solvent, a collagen extract could be obtained from animal-derived fat in a short time (about 6 hours), and about 2-3% of collagen by mass compared to the raw material could be obtained. The presence of collagen in the extract obtained by supercritical extraction was confirmed by SDS-PAGE, and it was confirmed that it was type 1 collagen having a relatively large molecular weight. In addition, the growth factors of IGF-1, bFGF, VEGF and NGF were analyzed to find out which growth factors were present in the collagen obtained by supercritical extraction, and it was found that these growth factors were contained in the extract. There was no significant difference in DNA content per mg of sample before and after supercritical treatment. Further in-depth studies are likely to be needed on decellularization technology using the supercritical process. In conclusion, the extracellular matrix obtained through the solvent extraction process using a supercritical fluid contains growth factors above a certain amount even after decellularization and removal of fat, so that it was found that not only biocompatibility is greatly increased, but also tissue regeneration can be rapidly induced.

• Parasites, Hosts and Diseases
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• v.29 no.4
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• pp.339-354
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• 1991

Localization and characterization of the antigenic components of sparganum which induced IgG and IsM antibodies in the host were studied by immunohistochemical techniques and SDS-PAGT and Western blotting. The antigen recognized by IgG antibody of rats or mice which were immunised by infection or injection of crude extracts of metacestodes of Spirometra erinacei, was located in the parenchyme of sparganum, especially at the cortex and around the calcareous corpuscles. The immunoreaction was demonstrated not only in the encysted fibrous wall of host but around the arterioles or venules in the connective tissue of host. The antigen recognized by IgM antibody of rats or mice was also observed in the parenchyme of sparganum and in the connective tissue of host. By 5∼20% gradient SDS-PAGE and EIBT, we detected antigenic components by IgG and 1gG antibodies of the rat or mouse immunized by infection or injection of crude extract of spargana. Twenty-three antigenic bands from crude extracts of spargana were recognized by IgG antibody and 15 components by IgM antibody of immunized rats. Out of the bands recognized by IgG and IgM antibodies, 15 were cross-reacted each other. Twenty components of eBlcretory-secretory proteins from spargana were recognized by IgG, and 5 components by IgM antibody of immunized rats. By IgG and IgM antibodies of immunized mice, 16 components of crude extracts were recognized by IgG antibody and 9 components by IgM antibody. Twenty components of excretory-secretory preparation were recognized by IgG antibody and 5 components by IgM antibody. Thirteen components of crude extracts were cross-reacted by IgG antibody of rats and mice.

• Journal of the Korean housing association
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• v.21 no.2
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• pp.19-30
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• 2010

The aim of this research is to understand the characteristics and status of existing public facilities, in order to propose the necessary basic materials for the planning and development of improved public facilities to assist communities. Objects of this research are the public facilities provided by the Government throughout the 25gu in Seoul. The methods of this research were carried out through the home page of each facility, telephone inquiries and gujung backseo. Investigations were carried out 3 times between the periods of January-June and September in 2009. Results of the research are as follows - 1) Through the investigations of classifications and distribution status of 5 public facilities over 25gu, quantity supply problems were analyzed. 2) Building distributions of 5 public facilities were classified by types and analyzed. 3) A number of compound facilities were classified and analyzed to determine the complexities of the 5 public facilities. 4) Complex types and characteristics of public facilities in 25gu in Seoul were analyzed. Based on the results of the research, the following conclusions are drawn: 1) Public facilities throughout the 25gu in Seoul have common characteristics and functions and they are spread out evenly in each gu. This means that public facilities are standardized in Seoul. Characteristic public facilities suitable to each gu should be planned accordingly. 2) Public facilities in Seoul show a single form of one facility. Therefore, initiative plans for the complexity of facilities are required. 3) Complex public facilities in Seoul need to be the center of community life in each gu. It is necessary to take into account the diverse life styles and classified needs of residents for complex facilities. Based on the research, complex types such as public administrative+cultural facilities, culture+welfare facilities, medical+welfare facilities can be presented. These complex types should not be classified according to any particular use, but they should be commonly used by diverse generations and every social class. The inquiries of users of public facilities and the programs of each public facility are not included in this research. Thus, the reseaarch limits the proposals for complex public facilities. In the future, the characteristics of each gu in Seoul should be studied and understood, so that appropriate public facilities and their complex plans can be researched continuously and necessarily.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.6
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• pp.122-130
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• 2013

The demand and the supply are increasing sharply in accordance with the growth of the Memory Semiconductor Industry. The Flash Memory above all is being utilized substantially in the Industry of smart phone, the tablet PC and the System on Chip (SoC). The Flash Memory is divided into the NOR-type Flash Memory and the NAND-type Flash Memory. A lot of study such as the Built-In Self Test (BIST), the Built-In Self Repair (BISR) and the Built-In Redundancy Analysis (BIRA), etc. has been progressed in the NOR-type fash Memory, the study for the Built-In Self Test of the NAND-type Flash Memory has not been progressed. At present, the pattern test of the NAND-type Flash Memory is being carried out using the outside test equipment of high price. The NAND-type Flash Memory is being depended on the outside equipment as there is no Built-In Self Test since the erasure of block unit, the reading and writing of page unit are possible in the NAND-type Flash Memory. The Built-In Self Test equipped with 2 kinds of finite state machine based structure is proposed, so as to carry out the pattern test without the outside pattern test equipment from the NAND-type Flash Memory which carried out the test dependant on the outside pattern test equipment of high price.

• Journal of Microbiology and Biotechnology
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• v.11 no.4
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• pp.592-597
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• 2001

The pyruvate dehydrogenase complex (PDC) catalyzes the oxidative decarboxylation of pyruvate with the formation of $CO_2$, acetyl-CoA, NADH, and H+. This complex contains multiple copies of three catalytic components including pyruvate dehydrogenase(E1), dihydrolipoamide acetyltransferase(E2), and dihydrolipoamide dehydrogenase (E3). Two regulatory components (E1-kinase and phospho-E1 phosphatase) and functionally less-understood protein (protein X, E3BP) are also involved in the formation of the complex. In this study, cloning and characterization of a gene for human E3BP have been carried out. A cDNA encoding the human E3BP was isolated by database search and cDNA library screening. The primary structure of E3BP has some similar characteristics with that of E2 in the lipoyl domain and the carboxyl-terminal domain, based on the nucleotide sequence and the deduced amino acid sequence. However, the conserved amino acid moiety including the histidine residue for acetyltransferase activity in E2 is not conserved in the case of human E3BP. The human E3BP was expressed and purified in E. coli. The molecular weight of the protein, excluding the mitochondrial target sequence, was about 50 kDa as determined by SDS-PAGE. Cloning of human E3BP and expression of the recombinant E3BP will facilitate the understanding of the role(s) of E3BP in mammalian PDC.

• Journal of KIISE:Computing Practices and Letters
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• v.14 no.1
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• pp.91-95
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• 2008

Flash memory is widely used in embedded systems because of its benefits such as non-volatile, shock resistant, and low power consumption. But NAND flash memory suffers from out-place-update, limited erase cycles, and page based read/write operations. To solve these problems, log-structured filesystem was proposed such as YAFFS. However, YAFFS sequentially retrieves an array of all block information to allocate free block for a write operation. Also before the write operation, YAFPS read the array of block information to find invalid block for erase. These could reduce the performance of the filesystem. This paper suggests fast operation method for NAND flash filesystem that solves the above-mentioned problems. We implemented the proposed methods in YAFFS. And we measured the performance compared with the original technique.

• Journal of the Korean Society for Library and Information Science
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• v.48 no.2
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• pp.25-44
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• 2014

This research evaluates the accessibility of the mobile applications for the South Korean libraries based on the accessibility guideline from the Ministry of Security and Public Administration. In order to enhance the credibility of the evaluation, this current research covers both the accessibility for the visually impaired and the accessibility for the people without disabilities. The research found four main results. First, we found that only 21 libraries (31%) provide alternative texts. Out of the 21 libraries, only one provide alternative texts across all sections of the mobile applications, including the main page, data search, information assistance, etc. Second, most of the mobile applications provide contents in texts, and the subtitles, sign language, blinking and background music provided as required or recommended standard by the guideline lack correlation. Third, alternative texts, focus movement, accessibility of operating system, button motion control, spacing between control and alarm functions must follow the standard guideline for the people with disabilities to use the mobile applications. Fourth, follow-up research on the development of accessibility standard for library mobile application is necessary in order to enable people with disabilities to freely use the library mobile applications.