• Korean Journal of Food Science and Technology
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• v.30 no.1
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• pp.1-5
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• 1998

To find out the difference between native bee-honey (NBH) and foreign bee-honey (FBH), quantification of honey protein and investigation of specific protein in NBH were carried out by SDS-PAGE. Contents of honey protein in NBH and FBH were measured by Bradford and Lowry method. The contents of protein determined by Bradford method were $0.1{\sim}3.3\;mg/g$ in NBH and $0.2{\sim}1.6\;mg/g$ in FBH, and by Lowry method were $12.9{\sim}45.7\;mg/g$ in NBH and $15.8{\sim}27.1\;mg/g$ in FBH. In order to investigate the distribution of bee honey proteins, the SDS-PAGE was performed. The results showed that molecular weight of the major proteins in NBH and in FBH were 56 kDa and 59 kDa. respectively. Therefore, it was confirmed that the difference between NBH and FBH can be identified visually by SDS-PAGE analysis. The major proteins in NBH and FBH were purified through two step chromatography, and the obtained proteins were used as marker protein in SDS-PAGE to discriminate NBH and FBH.

• Microbiology and Biotechnology Letters
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• v.23 no.1
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• pp.110-117
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• 1995

This study was carried out to immunologically characterize Bacillus thuringiensis (B.t) antigens. Protein patterns of ultrasonicated- antigens of B. thuringiensis subspecies using SDS- PAGE revealed marked similarities among all the strains analyzed except for the difference between quantative variations of bands and some protein antigens. The comparison of the protein patterns showed that the protein antigen of 45 kilodalton (kd) was common in 11 strains and that the difference between B. thuringiensis subsp. canadensis and galleriae was noticed in quantative variations of bands despite of ambiguous serogrouping, suggesting a useful method for identification. All strains examined showed similar antigenic patterns in SDS-PAGE, while immunodominant bands differed in antigenic reactivity in western blot using polyclonal antibodies. Polyclonal antibody to B. thuringiensis subsp. thuringiensis and israelensis in indirect immunofluorescence assay reacted with flagella and cell surface antigens. The present study indicates that SDS-PAGE and western blot analysis may be used as tools for differentiation and identification of B. thuringiensis subspecies.

• BMB Reports
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• v.28 no.4
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• pp.359-362
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• 1995

The highly polymorphic variable number of tandem repeat (VNTR) loci in the human genome are informative markers for the genetic characterization of individuals in the paternity test and forensic science as well as for the study of human disease. In this study, VNTR loci D1S80 and D2S123 have been amplified by PCR and the amplified length polymorphic alleles were detected with a discontinuous vertical PAGE system and silver staining. For explicit DNA typing, PCR optimization, in which amplification efficiencies are similar over a wide range of allele sizes, non-specific amplifications are minimal, and new longer alleles have high amplification efficiency, has been performed by changing the PCR reaction buffer composition and thermal cycling conditions. It turned out that adding an appropriate amount of Tween 20 and NP40 to the PCR reaction buffer and raising the annealing temperature to $68^{\circ}C$ in thermal cycling made it possible for optimal VNTR loci amplification. A modified PAGE system for VNTR separation was established. Under these conditions, new longer alleles in the 01580 locus were discovered and 025123 pattern changes in colorectal tumors were observed. These technical tips are valuable for detecting various amplified fragment length polymorphisms.

• The KIPS Transactions:PartB
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• v.11B no.6
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• pp.723-734
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• 2004

In this paper, we present an automated Web page classification system based upon ontology. As a first step, to identify the representative terms given a set of classes, we compute the product of term frequency and document frequency. Secondly, the information gain of each term prioritizes it based on the possibility of classification. We compile a pair of the terms selected and a web page classification into rules using machine learning algorithms. The compiled rules classify any Web page into categories defined on a domain ontology. In the experiments, 78 terms out of 240 terms were identified as representative features given a set of Web pages. The resulting accuracy of the classification was, on the average, 83.52%.

• Journal of IKEEE
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• v.19 no.3
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• pp.424-430
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• 2015

This paper proposes an optical cryptography application to 2-D page-oriented data based on CFB(Cipher Feedback) mode algorithm. The proposed method uses a free-space optical interconnected dual-encoding technique which performs XOR logic operations in order to implement 2-D page-oriented data encryption. The proposed method provides more enhanced cryptosystem with greater security strength than the conventional CFB block mode with 1-D encryption key due to the huge encryption key with 2-D arrayed page type. To verify the proposed method, encryption and decryption of 2-D page data and error analysis are carried out by computer simulations. The results show that the proposed CFB optical encryption system makes it possible to implement stronger cryptosystem with massive data processing and long encryption key compared to 1-D block method.

• Parasites, Hosts and Diseases
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• v.31 no.4
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• pp.353-362
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• 1993

The present study was carried out to investigate the localization and isozyme patterns of acid phosphatase and alkaline phosphatase in metacercariae and in adults of F. seoulensis by enzyme-histochemistry method and electrophoresis. Acidphosphatase showed a strong activity at pH 5 in the intestinal caecum of adults, but showed no reactions in the nonsubstrate control and in the inhibitor-treated control. Alkaline phosphatase showed a strong activity at pH 8 in the intestinal caecum and the tribocytic organ of adults, and in the intestinal caecum and in the genital anlagen of metacercariae. In non-denature PAGE, ten bands of protein fraction from the extracts of metacercariae and twenty-two bands from adults were detected. In denature PAGE, two protein bands having molecular weights of 192 kDa and 123 kDa were detected in the metacercariae, but absent from adult stage. In adults, protein fractions of 27.5 kDa, 24.5 kDa, 21.4 kDa, 18 kDa, 16 kDa and 15 kDa were detected. In non-denature PAGE, isozymes of acid phosphatase showed the most strong activity at pH 5, whereas no activity was shown at pH 2 and pH 7. One isozyme 85 kDa, 73 kDa and 62 kDa) in adults.

• Korean Journal of Artificial Intelligence
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• v.11 no.2
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• pp.1-6
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• 2023

This paper presents a comprehensive scheme for assessing the importance of multiple microgrids (MGs) network that includes distributed energy resources (DERs), renewable energy systems (RESs), and energy storage system (ESS) facilities. Due to the uncertainty of severe weather, large-scale cascading failures are inevitable in energy networks. making the assessment of the structural vulnerability of the energy network an attractive research theme. This attention has led to the identification of the importance of measuring energy nodes. In multiple MG networks, the energy nodes are regarded as one MG. This paper presents a modified PageRank algorithm to assess the importance of MGs that include multiple DERs and ESS. With the importance rank order list of the multiple MG networks, the core MG (or node) of power production and consumption can be identified. Identifying such an MG is useful in preventing cascading failures by distributing the concentration on the core node, while increasing the effective link connection of the energy flow and energy trade. This scheme can be applied to identify the most profitable MG in the energy trade market so that the deployment operation of the MG connection can be decided to increase the effectiveness of energy usages. By identifying the important MG nodes in the network, it can help improve the resilience and robustness of the power grid system against large-scale cascading failures and other unexpected events. The proposed algorithm can point out which MG node is important in the MGs power grid network and thus, it could prevent the cascading failure by distributing the important MG node's role to other MG nodes.

• KIPS Transactions on Computer and Communication Systems
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• v.11 no.12
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• pp.419-428
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• 2022

Since in-place updates for pages are not allowed in flash memory, all new page writes should be written in an out-of-place manner. The old overwritten pages are invalidated. Such invalidated pages eventually trigger the costly garbage collection process. Since the garbage collection causes numerous read and write operations, it is one of the flash memory's major performance issues. In 2R, it modified the garbage collection algorithm, which applies the I/O characteristics of the On-Line Transaction Process workload to improve the Write Amplification Factor. However, this algorithm has a region pollution problem. Therefore, in this paper, we developed 2R++ that additionally separates pages with long access intervals to solve the region pollution problem. 2R++ introduces an extra bit per block to separate warm pages based on a second chance mechanism. Prevents warm pages from being misidentified as cold pages to solve region pollution problem. We conducted the experiments on TPC-C and Linkbench to make the performance comparison. The experiment showed that 2R++ achieved a Write Amplification Factor improvement of 57.8% and 13.8% compared to 2R, respectively.

• Journal of Korea Game Society
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• v.2 no.1
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• pp.16-22
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• 2002

After that Internet was introduced to Korea, Web page has developed from text centered to graphic-centered at its fist stage. At the present, it is improving to a design for users. Furthermore, with the acceleration of Information super-highway construction and generalization of basic technology for multi-media, the educational environment has transformed to demander-focused and internet basis service which transcends time and space. Consequently, the educational structure is converting from instructors unilateral lead to student-centered. Additionally, the common usage and digitalization of information have an effect on progress of education quality and cost saving. Although there are plenty of educational web pages on internet, we can notice that many of them are inconvenient for users to put into practice. The reason is that many experts overlook the fundamental which is the basic skill for design and understanding of Web must be accompanied with Web design. Therefore, this thesis will find out the points users should consider for use of Web page and realize the educational web page, reflected for VRML training.

• Korean Journal of Medicinal Crop Science
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• v.3 no.1
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• pp.61-65
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• 1995

Karyotype analysis was carried out in four lines of Bupleurum falcatum L. cultivated in Korea and SDS-PAGE was applied to determine the seed protein profiles among the lines. Chromosomes were classified into two groups, large and small ones. Two kinds of karyotype, 2n=20 and 2n=26, were identified. Chromosome 1 of 2n=20 were all submedian, while that of 2n=26 were median. Chromosomes 2, 3 and 5 of 2n=20 showed polymorphism in size and arm-ratio. Chromosome 2 was submedian, while others were median in the line of 2n=26. Karyotypcs of cultivars native of Korea were similiar each other, while those introduced from Japan showed different patterns. In SDS PAGE gels, qualitative difference s in high molecular weight proteins, more than 45KD, were detected among the lines. The numbers of specific band were three in lines of 2n=20 and two in 2n=26.