• Archives of Pharmacal Research
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• v.24 no.5
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• pp.446-454
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• 2001

Peptide fragments, isolated from proteolytic cleavage of thyroglobulin at specific sites, were examined for the iodination of tyrosine residues. The 50 kDa polypeptide, which was prepared from digestion of bovine thyroglobulin and continuous preparative SDS-PAGE, was subjected to reduction with DTT and alkylation with iodoacetic acid to generate S-car-boxymethylated peptide derivative, which was further hydrohysed by endoproteinase-Asp-N. Peptide products were separated by RP-HPLC, and each fraction was analyzed by LC/ESI-MS and MALDI-MS analyses. Based on the specificity of endoproteinase-Asp-N andthe mass spectra data, a peptide fragment turned out to correspond to a peptide, DALCCVKCPEGSYFQ (1438-1452), characterized by the presence of a thioredoxin box (CVKC) and a tyrosine residue. In addition, another peptide fragment (1453-1465) containing a thioredoxin box (CIPC) and a tyrosine residue was also observed. However, any evidence of iodination of the tyrosine residue present in these peptides was not provided. Meanwhile, tyrosine residues in the peptides, DVEEALAGKYLAGRFA (1366-1381) and DYSGLLLAFQVFLL (1290-1303) were found to be iodinated; mono- or diiodinated tyrosine residues, characteristic of a hormogenic site, existed in both peptides. In addition, the tyrosine residue in the peptide (1218-1252), corresponding to a hormonogenic site was also iodinated. Thus, there was a sharp difference of the susceptibility to oxidative iodination between the tyrosine residue in a hormonogenic site and that in a thioredoxin region. From these results, it is suggested that polypeptide region adjacent to tyrosine residues may govern the susceptibility of tyrosine to oxidative iodination.

• Parasites, Hosts and Diseases
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• v.37 no.1
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• pp.39-46
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• 1999

As a preliminary study for the explanation of pathobiology of Neodiplostomum seoulense infection. a 54 kDa protease was purified from the crude extract of adult worms by sequential chromatographic methods. The crude extract was subjected to DEAE-Sepharose Fast Flow column, and protein was eluted using 25 mM Tris-HC1 (pH 7.4) containing 0.05. 0.1, 0.2 and 0.4 M NaC1 in stepwise elution. The 0.2 M NaCl fraction was further purified by Q-Sepharose chromatography and protein was eluted using 20 mM sodium acetate (pH 6.4) containing 0.05, 0.1. 0.2 and 0.3 M NaCl, respectively. The 0.1M NaCl fraction showed a single protein band on SDS-PAGE carried out on a 7.5-15% gradient gel. The proteolytic activities of the purified enzyme were specifically inhibited by L-trans-epoxy-succinylleucylamide (4-guanidino) butane (E-64) and iodoacetic acid. The enzyme, cysteine protease. showed the maximum proteolytic activity at pH 6.0 in 0.1 M buffer, and degraded extracellular matrix proteins such as collagen and fibronectin with different activities. It is suggested that the cysteine protease may playa role in the nutrient uptake of N. seoulense from the host intestine.

• Fashion & Textile Research Journal
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• v.11 no.2
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• pp.308-314
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• 2009

In creating an avatar, consumers can find out for themselves their own sizes to be entered. Also, putting in a face creation main-page menu option enables clients to make up their faces and match them to their bodies. Through this process when purchasing clothes through the internet consumers can enter their own body sizes and create avatars that are identical to their body shape. The uniqueness of developed prototype is that it creates an avatar similar to one's body shape according to body size inputs and demonstrates visually to customers the on-line comfort test which was only available off-line traditionally. The avatar follows the movement of mouse from left to right which enables 3 dimensional visualization. Through maximizing the visual effect this research focuses on enabling consumers to feel subjective parts as if they are off-line. This study seeks to provide a type of prototype of an online shopping mall that meets the demand of the consumers using the 3D avatar.

• International Journal of Contents
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• v.8 no.1
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• pp.69-73
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• 2012

This paper aims to present colour theories and show how they can be used to explain consumer's preferences of some products over others. It will, furthermore, attempt to link these theories to the design industry and look at how colour associations determine product success. Due to associative learning and personal preference, the colours of objects can cause consumers to either favour or dislike products over those with identical functions and efficiency. Age and gender affect the preferred colour choices of the individual, making some products more popular to particular groups of potential consumers. Designers can utilise colour theories to ensure that they use the most appropriate colour schemes to maximise and appeal to their targeted market successfully. A survey was conducted with 100 participants. It demonstrates the associative links between colours, emotions and product categories. It can be shown that the colour of an object can contribute to its success or failure in the market based on a number of different criteria. The design must use colour confidently to evoke certain emotions or connotations and must be carried out appropriately. The designer also has to consider whom it is that be viewing it and making the decision of preference.

• Journal of the Korea Convergence Society
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• v.6 no.5
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• pp.33-38
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• 2015

In a V2X convergence service environment, the principal service among infotainment services and driver management services must be supported centering on critical information of the driver, maintenance manager, customer, and anonymous user. Many software applications have considered solutions to be satisfied the specific requirements of driving care programs, and plans. This paper describes data flow diagram of a secure clinic system for driving car diagnosis, which is included in clinic configuration, clinic, clinic page, membership, clinic request processing, driver profile data, clinic membership data, and clinic authentication in the V2X convergence service environment. It is reviewed focusing on security threat issue of ITS diagnostic system such as spoofing, tampering, repudiation, disclosure, denial of service, and privilege out of STRIDE model.

• Korean Journal of Veterinary Research
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• v.44 no.2
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• pp.269-277
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• 2004

Trichinella spiralis is one of the important zoonotic parasites with a wide variety of vertebrates hosts in nature. The purpose of this study were to analyze ESP(Excretory-Secretory Protein) antigen, to evaluate ELISA for the serological diagnosis of Trichinosis, and to survey T. spiralis infection in finishing pigs using the pepsin digestion method and ELISA in Korea. In the analysis of ESP antigen by SDS-PAGE and Western blot, 4 major bands (70, 55, 52.6, and 49 kDa) were revealed from the ESP antigen. Predilection sites of T. spiralis were the diaphragm, the tongue, masseter muscles, intercostal muscle, and hindlimb in orders in the experimentally infected rats. Sera from 581 swine were tested by ELISA with ESP antigen. The 54 (9.3%) sera were suspected as positive reactors, however, these 54 sera were determined as false positives by the use of Western blotting. This study demonstrated that the ELISA was not suitable for the examination of T. spiralis in pork. The diaphragm muscle samples of 251 finishing pigs were tested by the method of pepsin-digestion for the presence of Trichinella larvae, however, T. spiralis was not detected from the samples. We could not find out T. spiralis infection in pig in Korea pork.

• International Journal of Industrial Entomology and Biomaterials
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• v.34 no.1
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• pp.6-10
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• 2017

Dissolution of Antheraea yamamai silkworm cocoon was carried out in various solvent systems with various dissolving conditions including dissolution salts, salt concentration, dissolving temperature, and time. General chaotropic salt for Bombyx mori silk fibroin does not work for A. yamamai silkworm cocoon. Lithium bromide 9.3 M at $100^{\circ}C$ also does not work to dissolve wild silkworm cocoon. However, 9 M of lithium thiocyanate treatment at $100^{\circ}C$ induced 100% dissolution of wild silkworm cocoon. But it could not be dissolved lower than $60^{\circ}C$. Like lithium thiocyanate, less than $60^{\circ}C$ treatment with molten calcium nitrate 4 hydrate could not dissolve wild silkworm cocoon. As the dissolution temperature increased up to $100^{\circ}C$, the solubility of wild one was reached over 90%. SDS-PAGE showed broad tailing stream pattern that means the molecule of wild silk was depolymerized with dissolution temperature and time. From the above results, the best chaotropic salt for A.yamamai silkworm cocoon is calcium nitrate 4 hydrate.

• Parasites, Hosts and Diseases
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• v.44 no.1 s.137
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• pp.43-48
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• 2006

Experimental murine models with high, intermediate and low levels of genetically based susceptibility to Leishmania major infection reproduce almost entire spectrum of clinical manifestations of the human disease. There are increasing non-comparative studies on immune responses against isolated antigens of L. major in different murine strains. The aim of the present study was to find out whether there is an antigen that can induce protective immune response in resistant and susceptible murine strains. To do that, crude antigenic extract of procyclic and metacyclic promastigotes of L. major was prepared and subjected to SDS-PAGE electrophoresis. Western-blotting was used to search for antigen(s) capable of raising high antibody level of IgG2a versus IgG1 in the sera of both infected resistant and susceptible strains. Two novel antigens from metacyclic promastigotes of L. major (140 and 152 kDa) were potentially able to induce specific dominant IgG2a responses in BALB/c and C57BU6 mice. The 2 antigens also reacted with IgG antibody of cutaneous leishmaniasis patients. We confirm that 140 and 152 kDa proteins of L. major promastigotes are inducing IgG production in mice and humans.

• Magazine of the Korean Society of Agricultural Engineers
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• v.31 no.1
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• pp.58-70
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• 1989

The paper compared the Bishop methed to the Fellenius method in the analysis of slope stability. Laboratory model test was carried out in the case of seepage flow considered. The results obtained from this study were summarized as follows; 1. The slice pieces of 10 were enough to analysis the slope stability. 2. The safety factor. by the Fellenius method was lower than the Bishop method by the 96 to 97% in the case of no seepage flow and by the 95 to 96% in the case of seepage flow considered. 3. Besides the parameter of soil and slope, the safety factor of slope was influenced by the height of slope. This phenomena was distinct in the height of height less than 10 meters. 4. In the case of clay, there was no difference in the safety factor of slope between Fellenius and Bishop rnethod. The safety factors of slope with the seepage flow considered were lower than those with no see-page flow. 5. The influence of cohesion on the safety factor was more significant in the Bishop method than in the Fellenius method. 6. The slope failure of model test of A and B soil samples with high permeability coefficient was taken place slightly in vicinity of toe by the concentration of stress and gradually increased 7. Under condition of same slope height, the shapper the slope, the shorter the radius and the center of critical circle appered downward and finally failure of slope occured inside the slope.

• Journal of the Korean Society of Clothing and Textiles
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• v.31 no.11
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• pp.1586-1597
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• 2007

This study is an attempt to examine fashion elements appearing in the cover-page design of fashion magazines published in Korea and aims to find Korea#s own fashion identity in fashion styling and designing of those fashion magazines. In order to do so, the study reviewed the related literature and analyzed the issues of Vogue and Harper#s Bazaar magazines published between 2004 and 2006. The results of the study can be summarized as follows: In case of fashion photographs, the largest number of 72 sampled cover-model photographs is in approximately three-quarter cut size. For the items, most take the form of one-piece dress and feature the use of a variety of accessories. In case of dresses, most are the creations of foreign designers and famous fashion models or celebrities show up, mostly alone, as features on the cover pages. Because of the nature of fashion magazines, their primary emphasis is put on the dress among other things, but on the other hand some of those magazines have differential cover pages where the model#s face is highlighted with the look of makeup or a famous female actress stands out. However, the fashion in designing the cover pages of magazines is, rather than to show the dress itself, to create a new combination of different elements as total fashion or convey an image based on such a fashion style.