• Analytical Science and Technology
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• v.8 no.4
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• pp.865-868
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• 1995

The method of matrix-assisted laser desorption ionization mass spectrometry has been used to solve some bioanalytical problems, which is difficult to analyse by general methods. For the selection of proper laser wavelength and matrices, eight matriees was used with laser wavelength of 226 and 355nm. The result shows that with wavelength of 355nm better results could be obtained with most of the matrices. The molecular weight of eytochrome C, which was seperated by gel electrophoresis and electro-blotted onto NC membrane is determined by MALDI. The accuracy is better than 0.1%, which is much higher than that of SDS-PAGE. Protein mixture extracted from crude peanut oil is directly determined by MALDI. The molecuiar weight of its three components are determined, and the result also demonstrated that these proteins are in free manner. As proteins arc in 2S bond, with the traditional method, SDS-PAGE, it is not able to decide whether protein exists in combination mode or in free manner. In the technique of two phase aquesous solution, which is used for separating biomaterials, water soluble polymers stained with dyes are used in this technique. By the use of MALDI the number or the dye molecules react with the polymer PEG molecule are determined, and that is difficult to determined by other methods.

• Microbiology and Biotechnology Letters
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• v.38 no.2
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• pp.163-167
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• 2010

Bacillus subtilis 168 secreted antimicrobial substance(s) into culture medium and culture supernatant inhibited growth of some Gram positive bacteria. B. cereus and Listeria monocytogenes were the most sensitive organisms. The antimicrobial activity was destroyed when culture supernatant was treated by protease and proteinase K, indicating the proteinous nature of the substance (bacteriocin). The molecular weight of the bacteriocin was estimated to be 5 kDa by Tricine SDS-PAGE. B. cereus ATCC 14579 cells were killed when exposed to the bacteriocin, indicating that the mode of inhibition was bacteriocidal. These results show that B. subtilis 168 could be useful as a starter for fermented foods such as cheonggukjang where B. cereus contamination is a major concern.

• Journal of the Korea Society of Computer and Information
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• v.25 no.2
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• pp.21-29
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• 2020

The use of NAND flash memory is continuously increased with the demand of mobile data in the IT industry environment. However, the erase operations in flash memory require longer latency and higher power consumption, resulting in the limited lifetime for each cell. Therefore, frequent write/erase operations reduce the performance and the lifetime of the flash memory. In order to solve this problem, management techniques for improving the performance of flash based storage by reducing write and erase operations of flash memory with using disk buffers have been studied. In this paper, we propose a CPWL to minimized the number of write operations. It is a disk buffer management that separates read and write pages according to the characteristics of the buffer memory access patterns. This technique increases the lifespan of the flash memory and decreases an energy consumption by reducing the number of writes by arranging pages according to the characteristics of buffer memory access mode of requested pages.

• Korean journal of communication and information
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• v.16
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• pp.207-254
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• 2001

This study is focused on forecasting the future of tole-democracy. Many Scholars expect that internet provides technological space needing realizing the tole-democracy or deliberate democracy. Especially in Korea, this expectation is higher than other nations because of political corruption and inefficiency. Therefore internet is intended to considering as new technologies reforming political process. In 16th the general election period, many candidates established home page and used as election campaign tool. And a little of home pages is payed attention to among voters. In spite of using internet as political medium, many political communication researchers have a doubt that internet will realize ideal direct democracy. It's reason is that internet is open and anonymous space. At anonymous space, communication participators is tended to be irresponsible and non-serious. Therefore it is hard that cyber-politics will be ideal type of democracy. In this context, this paper analyzed how pauicipators communicate with others at cyber bulletin board establishing candidate's home pages. Main research questions is how do discussions at cyberspace fulfill the conditions of deliberate democracy. Therefore, concrete questions include; who are participators at candidate's cyber bulletin board; which pattern do they communicate; what is the theme of communication; which effects do the anonymous character of cyberspace influence. To that purpose, this study conducted content analysis on 4,210 written matters at 82 cyber bulletin boards of candidate's home page establishing during 16th the general election period. It can be found that cyberspace in Korea still is not deliberate democracy space and not will be. Firstly, discussion patterns at cyber bulletin board is "candidate with supporters communication space. To be exact, it is said that candidate's home page is "the space of self-convincing among supporters." Secondly, the main themes of discussion are simple emotional expressions; "I support you!" "fighting" "Be vigor" etc. By contrast, real political contents'-central or local political matters - is relatively few. In the mode of expression, real political messages are more positive, logical than simple expressions, candidates private matters. Especially this characteristic will make cyberspace as "mutual slander space" consolidating anonymous characteristic of cyberspace. finally, Cyberspace in Korea still is not real "public sphere" realizing deliberate process. Therefore to be real public sphere, it is needed to participant's ethical maturity and political citizenship. In conclusion, it is difficult that cyberspace will reconstruct the Athene's Agora. On the contrary, Cyberagora will like to be irrespectable area fulfilling the sweeping. Making the deliberate space, technological possibility and ethical condition will have to be balanced together.

• Journal of Intelligence and Information Systems
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• v.22 no.2
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• pp.57-79
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• 2016

It is a rising trend that the number of users using one of the social media channels, the Social Network Service, so called the SNS, is getting increased. As per to this social trend, more companies have interest in this networking platform and start to invest their funds in it. It has received much attention as a tool spreading and expanding the message that a company wants to deliver to its customers and has been recognized as an important channel in terms of the relationship marketing with them. The environment of media that is radically changing these days makes possible for companies to approach their customers in various ways. Particularly, the social network service, which has been developed rapidly, provides the environment that customers can freely talk about products. For companies, it also works as a channel that gives customized information to customers. To succeed in the online environment, companies need to not only build the relationship between companies and customers but focus on the relationship between customers as well. In response to the online environment with the continuous development of technology, companies have tirelessly made the novel marketing strategy. Especially, as the one-to-one marketing to customers become available, it is more important for companies to maintain the relationship marketing with their customers. Among many SNS, Facebook, which many companies use as a communication channel, provides a fan page service for each company that supports its business. Facebook fan page is the platform that the event, information and announcement can be shared with customers using texts, videos, and pictures. Companies open their own fan pages in order to inform their companies and businesses. Such page functions as the websites of companies and has a characteristic of their brand communities such as blogs as well. As Facebook has become the major communication medium with customers, companies recognize its importance as the effective marketing channel, but they still need to investigate their business performances by using Facebook. Although there are infinite potentials in Facebook fan page that even has a function as a community between users, which other platforms do not, it is incomplete to regard companies' Facebook fan pages as communities and analyze them. In this study, it explores the relationship among customers through the network of the Facebook fan page users. The previous studies on a company's Facebook fan page were focused on finding out the effective operational direction by analyzing the use state of the company. However, in this study, it draws out the structural variable of the network, which customer committment can be measured by applying the social network analysis methodology and investigates the influence of the structural characteristics of network on the business performance of companies in an empirical way. Through each company's Facebook fan page, the network of users who engaged in the communication with each company is exploited and it is the one-mode undirected binary network that respectively regards users and the relationship of them in terms of their marketing activities as the node and link. In this network, it draws out the structural variable of network that can explain the customer commitment, who pressed "like," made comments and shared the Facebook marketing message, of each company by calculating density, global clustering coefficient, mean geodesic distance, diameter. By exploiting companies' historical performance such as net income and Tobin's Q indicator as the result variables, this study investigates influence on companies' business performances. For this purpose, it collects the network data on the subjects of 54 companies among KOSPI-listed companies, which have posted more than 100 articles on their Facebook fan pages during the data collection period. Then it draws out the network indicator of each company. The indicator related to companies' performances is calculated, based on the posted value on DART website of the Financial Supervisory Service. From the academic perspective, this study suggests a new approach through the social network analysis methodology to researchers who attempt to study the business-purpose utilization of the social media channel. From the practical perspective, this study proposes the more substantive marketing performance measurements to companies performing marketing activities through the social media and it is expected that it will bring a foundation of establishing smart business strategies by using the network indicators.

• JSTS:Journal of Semiconductor Technology and Science
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• v.2 no.3
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• pp.173-179
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• 2002

We propose a novel sensing circuit for 2T-2MTJ MRAM that can be used for high speed synchronous operation. Proposed bit-line sense amplifier detects small voltage difference in bit-lines and develops it into rail-to-rail swing while maintaining small voltage difference on TMR cells. It is small enough to fit into each column that the whole data array on selected word line are activated as in DRAMs for high-speed read-out by changing column addresses only. We designed a 256Kb read-only MRAM in a $0.35\mu\textrm{m}$ logic technology to verify the new sensing scheme. Simulation result shows a 25ns RAS access time and a cycle time shorter than 10 ns.

• Journal of Microbiology and Biotechnology
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• v.1 no.2
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• pp.85-89
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• 1991

The aim of this study is to elucidate characteristics of ${\beta}-lactamase$ inhibitor produced by Streptomyces sp. SMF-19 isolated from soil was found to produce proteinaceous extracellular ${\beta}-lactamase$ inhibitor. The ${\beta}-lactamase$ inhibitor was purified through ammonium sulfate fractionation, gel filtration, anion exchange chromatography and fast performace liquid chromatography. The molecular weight of the ${\beta}-lactamase$ inhibitor was estimated to be about 48,000 by SDS-PAGE. The mode of inhibition against penicillin G as a substrate was uncompetitive. The ${\beta}-lactamase$ inhibitor was stable in wide pH range but unstable at high temperature above $50^{\circ}C$.

• Journal of KIISE:Computer Systems and Theory
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• v.35 no.6
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• pp.293-303
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• 2008

As the computing environment moves to the wireless and handheld system, the power efficiency is getting more important. That is the case especially in the embedded hand-held system and the power consumed by the memory system takes the second largest portion in overall. To save energy consumed in the memory system we can utilize low power mode of SDRAM. In the case of RDRAM, nap mode consumes less than 5% of the power consumed in active or standby mode. However hardware controller itself can't use this facility efficiently unless the operating system cooperates. In this paper we focus on how to minimize the number of active units of SDRAM. The operating system allocates its physical pages so that only a few units of SDRAM need to be activated and the unnecessary SDRAM can be put into nap mode. This work can be considered as a generalized and system-wide version of PAVM(Power-Aware Virtual Memory) research. We take all the physical memory into account, especially buffer cache, which takes an half of total memory usage on average. Because of the portion of buffer cache and its importance, PAVM approach cannot be robust without taking the buffer cache into account. In this paper, we analyze the RAM usage and propose power-aware page allocation policy. Especially the pages mapped into the process' address space and the buffer cache pages are considered. The relationship and interactions of these two kinds of pages are analyzed and exploited for energy saving.

• Microbiology and Biotechnology Letters
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• v.48 no.2
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• pp.148-157
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• 2020

Eucalyptus oil is a rich source of bioactive compounds with a variety of biological activities and is widely used in traditional medicine. Eucalyptus citriodora is cultivated for the production of essential oils. However, the mode of antibacterial action of essential oils from E. citriodora is not well-known. This study aimed to determine the chemical components, microbial inhibitory effect, and mechanism of action of the essential oil from E. citriodora. The oil was extracted from E. citriodora leaves by hydro-distillation and the chemical components were analyzed using gas chromatography-mass spectrometry. The antibacterial activities of eucalyptus oil against gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus, and Staphylococcus intermedius) and gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) were screened by disc diffusion method and quantitative analysis was conducted by the microdilution method. The mechanism of action of the extracted essential oil was observed using SEM and analyzed by SDS-PAGE. The major components of E. citriodora oil were citronellal (60.55 ± 0.07%), followed by dl-isopulegol (10.57 ± 0.02%) and citronellol (9.04 ± 0.03%). The antibacterial screening indicated that E. citriodora oil exhibited prominent activity against all tested strains. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against B. subtilis were 0.5% and 1.0%, respectively. The MIC and MBC concentrations against S. aureus, S. intermedius, E. coli, and P. aeruginosa were 1% and 2%, respectively. As observed by SEM, the antibacterial mechanism of E. citriodora oil involved cell wall damage; SDS-PAGE revealed decrease in protein bands compared to untreated bacteria. Thus, E. citriodora oil showed significant antimicrobial properties and caused cellular damage.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.8 no.3
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• pp.614-621
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• 2004

At present, the electronic document is being processed in WYSWYG mode. For this, a document is structured by the logical structure and the physical structure, and is presented by the markup language. After XML is announced, an application scope of the electronic document is extended from interchanging to searching. However, in point of output quality, a XML document image on a browser has lower quality than a general document image on desktop publishing. The reason is which output function of a browser has not capability for high quality printing. The W3C developed XSL-FO(XSL-formatting Object) for style sheet formatting and PDL(Page Description Language) as like Postscript is already developed and used widely. In this paper, we designed the Postscript-Converter to get a high quality document image by converting XSL-FO into Postscript format.