• Women's Health Nursing
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• v.14 no.3
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• pp.189-195
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• 2008

Purpose: This study aimed to investigate the factors of occurrence of amenorrhea and the severity of climacteric symptoms in breast cancer patients who underwent chemotherapy. Method: Women diagnosed with breast cancer without metastasis or recurrence, had surgery followed by chemotherapy, and had menses at the time of surgery were recruited from S hospital located in Seoul. A total of 99 women aged 31 thru 55 years participated and filled out a structured questionnaire including the Functional Assessment Cancer Therapy-Breast plus Endocrine Symptom when they visited the clinic for follow-up. Result: In 88 women amenorrhea occurred within an average of 2 months since beginning chemotherapy, and menstruation was resumed in only 11 women. About 98% of women aged over 40 experienced a cessation in menses thus age was an apparent factor of amenorrhea (Exp(B)=.76, p<.05). Presence of chronic disease (${\beta}=.25$, p<.05) and body weight change (${\beta}=.30$, p<.01) were significant factors influencing the severity of climacteric symptoms. Conclusion: Nurses need to have clinical evidences of menstrual changes due to breast cancer treatment. Information about premature menopause and climacteric symptoms should be provided according to women's health conditions so that they cope better during their survival.

• Animal cells and systems
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• v.12 no.3
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• pp.149-155
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• 2008

We have isolated and characterized Agrius convolvuli cDNA encoding a c-type lysozyme. The cDNA sequence encodes a processed protein of 139 amino acid residues with 19 amino acid residues amino-terminal signal sequence and 120 amino acid residues mature sequence. The amino acid residues responsible for the catalytic activity and the binding of the substrate are conserved. Agrius lysozyme has a high identity to Manduca sexta. Recombinant A. convolvuli lysozyme was expressed in Escherichia coli BL21(DE3) pLysS cells for pGEX 4T-1 expression vector. Their optimal conditions for the fusion protein expression and purification were screened. Lysozyme gene amplified with primers ACLyz BamHI and ACLyz XhoI was ligated into the pGEX 4T-1 vector, which contained the glutathione S-transferase(GST) gene for fusion partner. The fusion protein was induced by IPTG and identified by SDS-PAGE analysis. Molecular weight of the fusion protein was estimated to be about 45 kDa. Recombinant lysozyme, fused to GST, was purified by glutathion-Sepharose 4B affinity chromatography. Western blot analysis of this protein revealed an immunoreactivity with the anti-Agrius lysozyme.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.37A no.12
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• pp.1106-1114
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• 2012

In order to provide high data rate and real time services under maritime environment, link-level performance of ship ad-hoc network (SANET) based on 3GPP LTE and IEEE 802.11p (WAVE) specifications are investigated and discussed in this paper. The measured maritime channel, whose delay spread is longer than the length of guard interval (GI) of both 3GPP LTE and IEEE 802.11p specifications, is adopted for the link-level simulations. For the purpose of eliminating inter-symbol interference (ISI) due to insufficient GI length, double antenna pattern (DAP) scheme and advanced time-domain decision-feedback equalizer (DFE) are proposed for LTE and WAVE systems, respectively. The proposed DFE removes the ISI in a same manner as the residual inter-symbol interference cancellation (RISIC) algorithm, but the inter-carrier interference (ICI) is reduced via cyclicity removal instead of cyclicity restoration used in the RISIC algorithm. Compared with existing schemes, our proposed DFE is a robust technique to overcome the severe ISI channel which has a comparatively large delay spread. Based on simulation results, not only comparisons between systems are discussed, but also some reformative suggestions are given.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.21 no.7
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• pp.637-642
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• 2011

The DMT freight is judged that economic performance is good because can increase cargoes than existing freight. However, the existing freight cars, each with a different balance to the body structure is bogie because the vibrations may occur. Thus, by minimizing vibration over the existing freight securing the safety of the driving if you will not have major problems in cargoes. In this study, multi-body dynamic analysis tool, VI-Rail using the actually Gyeongbu Railroad line and an empty, full freight condition include curve radius, track irregularity, The DMT freight of the derailed wagons were assessed for safety analysis. Full and empty freight conditions for parity in the Gyeongbu Railroad line(Dongdaegoo${\leftrightarrow}$Kyungsan) derailment safety analysis, such as derailment coefficient and the wheel unloaded, echoing the curve and the orbit is affected by the irregularity was found. Full freight condition than the empty conditions showed a significant derailment safety. Overall, the limits of derailment coefficient(Q/P = 0.8) and wheel unload decrement limits(${\triangle}P/P$ = 0.6) is less safe with me confirmed that the derailment safety.

• Journal of agriculture & life science
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• v.46 no.2
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• pp.129-137
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• 2012

A carboxymethyl cellulase gene, cel5B, was cloned, sequenced, and expressed in Escherichia coli. pRCS20 in E. coli was identified from metagenomic cosmid library of cow rumen for cellulase activity on a carboxymethyl cellulose agar plates. Cosmid clone (RCS20) was partially digested with Sau3AI, ligated into BamHI site of pBluescript II SK+ vector, and transformed into E. coli $DH5{\alpha}$. The insert DNA of 1.3 kb was obtained, designated cel5B, which has the activity of hydrolyzation of CMC. The cel5B gene had an open reading frame (ORF) of 1,059 bp encoding 352 amino acids with a signal peptide of 48 amino acids and the conserved region, VIYEIYNEPL, belongs to the glycosyl hydrolase family 5. The molecular mass of Cel5B protein expressed from E. coli $DH5{\alpha}$ exhibited to be about 34 kDa by CMC-SDS-PAGE. The optimal pH was 8.0, and the optimal temperature was about $50^{\circ}C$ for its enzymatic activity.

• Journal of Plant Biotechnology
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• v.37 no.3
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• pp.313-318
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• 2010

To develop edible vaccines for swine, the embryogenic calli (type II) derived from HiII genotype were inoculated with A. tumefaciens strain C58C1 containing the binary vector pMYV611, 613, 616, and V621, 622 and 623 respectively. Six of those vectors carry nptII gene which confers resistance to paromomycin and apxIIA gene producing ApxII toxin which is generated in various serum types of A. pleuropneumoniae as a target gene. The 4,120 callus clones for pMYV611, 5,959 callus clones for pMYV613, 7,581 callus clones for pMYV616, 52,329 callus clones for V621, 48,948 callus clones for V622, and 56,188 callus clones for V623 were inoculated. The frequency of positive response clone was confirmed into range of 2.3% - 4.4% for each vectors by NPTII ELISA kit assay, and the selected callus clones of them were finally 3 callus clones from pMYV611 (0.07%), 4 callus clones from pMYV613 (0.07%), 2 callus clones from pMYV616 (0.03%), 51 callus clones from V621 (0.1%), 72 callus clones from V622 (0.15%), and 102 callus clones from V623 (0.18%) respectively. From the selected callus clones of each binary vector, the integration of the apxIIA gene into maize genome was detected from 2 plants of pMYV613 and 2 plants of V623 by Southern blot analysis.

• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.502-509
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• 2005

Sexual reproduction plays an important role in the biology and epidemiology of oomycete plant pathogens such as the heterothallic species Phytophthora infestans. Recent worldwide dispersal of A2 mating type strains of P. infestans resulted in increased virulence, gene transfer, and genetic variation, creating new challenges for disease management. To develop a genetic assay for mating type identification in P. infestans, we used the Amplified Fragment Length Polymorphism (AFLP) technique. The primer combination E+AT/M+CTA detected a fragment specific to A1 mating type (Mat-A1) of P. infestans. This fragment was cloned and sequenced, and a pair of primers (INF-1, INF-2) were designed and used to differentiate P. infestans Mat-A1 from Mat-A2 strains. The Mat A1-specific fragment was detected using Southern blot analysis of PCR products amplified with primers INF-1 and INF-2 from genomic DNA of 14 P. infestans Mat-A1 strains, but not 13 P. infestans Mat-A2 strains or 8 other isolates representing several Phytophthora spp. Southern blot analysis of genomic DNAs of P. infestans isolates revealed a 1.6 kb restriction enzyme (EcoRI, BamHI, AvaI)-fragment only in Mat-A1 strains. The A1 mating type-specific primers amplified a unique band under stringent annealing temperatures of $63^{\circ}C-64^{\circ}C$, suggesting that this PCR assay could be developed into a useful method for mating type determination of P. infestans in field material.

• Journal of Microbiology and Biotechnology
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• v.28 no.12
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• pp.2036-2045
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• 2018

An endo-${\beta}$-1,4-glucanase gene, cel5L, was cloned using the shot-gun method from Bacillus sp.. The gene, which contained a predicted signal peptide, encoded a protein of 496 amino acid residues, and the molecular mass of the mature Cel5L was estimated to be 51.8 kDa. Cel5L contained a catalytic domain of glycoside hydrolase (GH) family 5 and a carbohydrate-binding module family 3 (CBM_3). Chromatography using HiTrap Q and CHT-II resulted in the isolation of two truncated forms corresponding to 50 (Cel5L-p50) and 35 kDa (Cel5L-p35, CBM_3-deleted form). Both enzymes were optimally active at pH 4.5 and $55^{\circ}C$, but had different half-lives of 4.0 and 22.8 min, respectively, at $70^{\circ}C$. The relative activities of Cel5L-p50 and Cel5L-p35 for barley ${\beta}$-glucan were 377.0 and 246.7%, respectively, compared to those for carboxymethyl-cellulose. The affinity and hydrolysis rate of pNPC by Cel5L-p35 were 1.7 and 3.3 times higher, respectively, than those by Cel5L-p50. Additions of each to a commercial enzyme set increased saccharification of pretreated rice straw powder by 17.5 and 21.0%, respectively. These results suggest CBM_3 is significantly contributing to thermostability, and to affinity and substrate specificity for small substrates, and that these two enzymes could be used as additives to enhance enzymatic saccharification.

• Journal of the Korean Society of School Health
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• v.34 no.2
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• pp.115-122
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• 2021

Purpose: The purpose of this descriptive research is to identify how stress from Covid-19, health beliefs, and social support of college students affect health prevention. Methods: The subjects of the study were 128 university students, excluding health major students, at one university in D City. The survey was conducted from August 1 to 31, 2020. The survey questionnaire consists of 8 items on stress from COVID-19, 12 items adapted from a health belief measurement tool, 12 items from a social support measurement tool, and 11 items adapted from a tool that measures health preventive behaviors. The collected data were analyzed using the hierarchical multiple regression analysis method with SPSS 26.0. Results: In model 1, stress from COVID-19 was statistically significant (β=-.403, p=.003). Model 2 added four health belief factors into Model 1. Stress (β=-.419, p<.001), perceived severity (β=-.193, p=.030), and perceived barriers (β=-.182, p=.009) were statistically significant. In model 3, stress (β=-.413, p<.001), perceived barriers (β=-.147, p=.034), and social support (β=.194, p=.011) were statistically significant. The regression equation was significant (F=15.395, p=<.001) and the model's explanatory power was 53.1%. Conclusion: The results show that when college students had a high degree of health beliefs about COVID-19, the degree of health preventive behaviors was proportionally high. To make them practice preventive health behaviors, it is necessary to develop infection control education programs to improve health beliefs.

• Fashion & Textile Research Journal
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• v.7 no.2
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• pp.247-251
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• 2005

In this the study, the effects of the manufacturing process conditions on the properties Thick and Thin Diameter yarns(TTD yarns) prepared with hot plate device in the draw winder were determined. Physical properties including wet shrinkage, tenacity and elongation of the samples were measured and thick and thin effect was analysed with the evenness tester. The results were as the follows: There was little change the wet shrinkage of the TTD yarns in the range of $70^{\circ}C{\sim}80^{\circ}C$ of $R_1$ temperature(lower hot cylinder) with the same Hot Plate(H/P) temperature, but the wet shrinkage of the TTD yarns decreased 5-10% when $R_1$ temperature was $90^{\circ}C$. The wet shrinkage of the TTD yarns decreased with the H/P temperature at the same temperature of $R_1$. There was little effect of $R_1$ and H/P temperature on the tenacity of TTD yarns. The elongation of TTD yarns increased with $R_1$ temperature at the same H/P temperature. The elongation of TTD yarns increased little bit for the first time and then decreased above that temperature with increasing H/P temperature at the same $R_1$ temperature. The thick and thin effect on the TTD yarns was obvious in $110^{\circ}C$ of H/P temperature regardless of $R_1$ temperature, while there was no thick and thin effect on the TTD yarns in $140^{\circ}C$ of H/P temperature.