• Title/Summary/Keyword: pH10

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Effect of Temperature, Time and pH on the Extraction of Protein in a Chrysalis of Silk Worm. (누에 번데기의 단백질 추출에 관한 연구)

  • 조철형;차월석;차월석
    • KSBB Journal
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    • v.4 no.2
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    • pp.65-68
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    • 1989
  • In order to determin the optimum extraction condition for protein in a Chrysalis of Silk Worm, Temperature, Time, and pH variation were conducted in the extraction. N.S.I. and nitrogen contents of the extracts in this condition were identified by Kjeldaha method and a C.H.N. corder. The results were as follows; Crude protein and crude fat contents in the extracts were 23.34% and 15.61%, respectively. N.S.I. values with respect to the temperature variation were 6.7% at 6$0^{\circ}C$, 7.94% at 8$0^{\circ}C$ and 8.67% at 10$0^{\circ}C$ in the condition of pH 4, 1 hr. N.S.I. values in pH variation were 7.94% at pH4, 8.99% at pH6, 9.70% at pH8, 10.19% at pH 10 and 12.16% at pH 12 in the condition of 8$0^{\circ}C$, 1hr. N.S.I. values in extraction time variation were 8.67% in 1hr, 9.23% in 2hr and 9.76% in 3hr in the condition of 10$0^{\circ}C$, pH 4. The tendency of N.S.I. variation in this extration condition was reconfirmed also by a C.H.N. corder.

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The Rate of Superoxide Radical (${O_2}^-$.) Production in Normal Fenton's Reagent at Different pHs (펜톤반응에서 pH의 변화에 따른 superoxide radical (${O_2}^-$.)의 생성)

  • 김용수;공성호;김재호
    • Journal of Soil and Groundwater Environment
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    • v.7 no.2
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    • pp.73-81
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    • 2002
  • In normal Fenton's reagent, the reductive mechanism of carbon tetrachloride (CT) with superoxide radical (${O_2}^-$.) was observed and the rate of ${O_2}^-$. production was investigated as a function of $H_2O$$_2$ concentration and pH. As pH was increased, the rate of 1-hexanol degradation was rapidly decreased from 90% (at pH 3) to 5% (at pH 11). On the other hand, more degradation of carbon tetrachloride was observed at higher pH regimes indicating Fenton's reaction is an oxidant-reductant co-existing system at neutral pHs. The rate of $O_2^{-}$ . production was observed at different $H_2$$O_2$ concentrations and at different pHs. The rate increased from (45.3$\pm$7.8) x $10^{-6}$ M/s to (151.0$\pm$26.2) x $10^{-6}$ M/s ($294mM H_2$$O_2$) at pH 11: the rate 3150 increased from (22.1$\pm$3.8) x $10^{-6}$ M/s at pH 7 to (151.0$\pm$26.2) x $^10{-6}$ M/s at pH 11 with 294mM $H_2$$O_2$, These results showed that Fenton's reagent could be applied at wide pH regimes. Especially, carbon tetrachloride, which can not be easily adsorbed to soils and then can be dissolved into groundwater causing a cancer, could be efficiently treated by Fenton's reagent.reagent.

Effect of pH on Continuous Hydrogen Fermentation (연속반응실험에서 수소생성에 대한 pH 영향)

  • Lee, Young-Joon
    • Journal of Environmental Health Sciences
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    • v.30 no.2
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    • pp.149-153
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    • 2004
  • The influences of pH on hydrogen production were also investigated over the pH range from 4.1 to 8.0 at HRT 10 hours. The hydrogen content for the produced gas was changed from 41 to 71% with corresponding pHs throughout this experiment. The produced hydrogen/carbon dioxide ratio was not vary significantly up to 6.0, then steepenly increased with increases in the pH. The maximal hydrogen yield was found to be 3.16 $\ell$/g sucrose at pH 5.0. Acetate production yield increased with increased pH, but butyrate production yield decreased with increased pH. Biomass yield increased with increased pH.

Stabilization of Bacillus lichemiformis $\alpha$-Amulase by Modification with IO$_4$-Oxidized Soluble Starch (과요오드산 산화당에 의한 효소의 안정성)

  • 금종화
    • The Korean Journal of Food And Nutrition
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    • v.14 no.3
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    • pp.263-268
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    • 2001
  • NaIO$_4$-산화 전분당을 Bacillus licheniformis의 $\alpha$-아밀라아제의 반응시켜서 시프염기 형성으로 당단백질로 변형시켜서 안정성을 확인하였다. 10$0^{\circ}C$에서의 열안정성은 10분 뒤에, pH 9.7에서 변형한 효소 비변형 효소의 순으로 높았다. 그러나 변형 및 안정성에 $\alpha$-cyclodextrin($\alpha$-CD)을 사용한 결과 큰 차이는 나지 않았다. pH 8.0에서 $\alpha$-CD 존재하에 변형한 효소는 pH 8~11dml 알칼리쪽에서 가장 높은 안정성을 나타냈으나, pH 5~7사이에는 다른 효소보다 낮았다. pH 9.7에서 변형하지 않은 효소는 pH 5부터 pH 13까지 서서히 증가하였고 pH 9.7에서 $\alpha$-CD존재 하의 효소는 pH 5부터 7까지 증가하다가 그 후 pH13까지 서서히 감소하였다. $\alpha$-CD존재하의 비변형 효소는 pH 7과 10에서 피크를나타낸 다음 pH12이후에는 급격히 낮아졌다. 변형한 효소는 HPLC 의 유출시간이 빨라wu서 변형하지 않은 효소보다 분자량이 큰 것으로 나타났다. 분자량 크기는 비변형 효소

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Synthesis and characterization of microporous TS-1 zeolite(MFI) (Microporous TS-1 Zeolite(MFI)의 합성과 특성)

  • 강선명;이희수;김익진
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.9 no.3
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    • pp.309-314
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    • 1999
  • A microporous zeolite-type tianosilicate (TS-1), new catalysis elements, was synthesized by differents of the reactant solution pH. The range of reactant solution pH has from 10.0 to 12.4 TS-1 Zeolite (ETS-10), having a large pore (8~10 ${\AA}$), was synthesized at 10.4 of pH, since TS-1 Zeolite (ETS-4), having a small pore (3~5 ${\AA}$), was synthesized at 11.5 of pH. Also the two materials simultaneously existed at the intermediate pH. Crystallization, physico chemical characteristics of synthesized TS-1 Zeolite were investigated by XRD, XRF, SEM and FT-IR techniques.

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Analysis of naturally Dyed Textile Fabrics by using Aronia Extract (아로니아 추출물에 의한 직물의 천연염색과 염액의 Spectrum 분석)

  • Won, Ah Young;Kim, Jongjun
    • Journal of Fashion Business
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    • v.22 no.1
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    • pp.124-134
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    • 2018
  • This study examined the dyeing characteristics and spectrum of cotton and silk by using dyeing solution extracted from aronia. The value $L^{\ast}$ which indicates the brightness of cotton regardless of mordant increases with increasing pH from pH 3.5 to pH 10.5. The color difference value(${\Delta}E$) of the cotton was generally high in pH 3.5 regardless of mordant existence. The silk showed the highest ${\Delta}E$ value in pH 3.5 dye solution as cotton did. On the other hand, the silk with mordant dyed showed the highest ${\Delta}E$ value in pH 7. Silk fabrics dyed with Aronia solution turns out red in pH 3.5, blue in pH 7 and yellow in pH 10.5. This is because of the amino acid, one of the silk ingredients, combines with a part of anthocyanin to show blue. As the result of the spectrum measurement, the maximum absorption wavelength of Aronia solution was increased in the order of pH 10.5, pH 3.5 and pH 7, regardless of extraction temperature and mordant. The measurement results of color fastness to washing and color fastness to light are generally low. Therefore, there is a need for further study to improve color fatness in the future.

Effect of pH in Irrigation Water on the Growth, Yield, and Grain Quality of Rice (관개용수 pH가 벼 생육, 수량, 미질에 미치는 영향(I))

  • Choi, Sun-Hwa;Kim, Ho-Il;An, Yeul;Huh, Yoo-Man
    • Proceedings of the Korean Society of Agricultural Engineers Conference
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    • 2003.10a
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    • pp.551-554
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    • 2003
  • This study was carried out to investigate the effects of the pH of irrigation water on the growth, yield, and grain quality of rice. It acquire fundamental knowledges to set up irrigation water quality standards. The pot experiment was conducted with 5 treatments using irrigation waters with various pH values(control, 4, 6, 8, 10) and replicated four times with randomized block design. The results of this study showed that the uptake of N, P, and K, Ripened grain ratio and yield of rice tended to be reduced at the irrigation water of pH 4 and pH 10. P uptake, Ripened grain ratio and yield of rice at pH 4 water were significantly lower than the control. K uptake at pH 10 water was significantly lower than the control. Plant height, SPAD value and protein content of rice were not affected by the pH of irrigation water.

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Electrochemical Oxidation of Benzidine and Hydrazobenzene

  • Won Mi-Sook;Shim Yoon-Bo;Park Su-Moon
    • Bulletin of the Korean Chemical Society
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    • v.13 no.6
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    • pp.680-683
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    • 1992
  • The electrochemistry of benzidine and hydrazobenzene was studied in water-acetonitrile mixed solutions at various pHs and the results are reported. The cyclic voltammetric peak for the oxidation of benzidine shows a pH dependency of -62 mV/pH in the pH range of 0-3.5, no pH dependency between pH values of 3.5 and about 10.5, and of about -50 mV/pH between pH=10.50 and 14.0, indicating that oxidation mechanisms differ depending on the pH of the medium. However, the CV peak for the hydrazobenzene oxidation is shown to be independent of pH of the medium, suggesting that the proton is not involved in the rate limiting step of the electrochemical oxidation of hydrazobenzene to azobenzene. Results of in situ spectroelectrochemical experiments indicate that the oxidation products obtained during the oxidation of benzidine and hydrazobenzene depend on the result of dynamic equilibria taking place at various pHs.

Stability of Ascorbic Acid in the Catechin Solution (Catechin용액 중에서 Ascorbic acid의 안정성)

  • Kim, Sang Oak
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.12 no.3
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    • pp.284-288
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    • 1983
  • This experiment was carried out to investigate the stability of ascorbic acid (AsA) in the mixed solution of AsA and catechin according to the content changes of AsA and catechin under boiling, and for the respective concentration and pH. The loss of AsA was most for 10 minutes of boiling and accelerated by the mixture of catechin, but the loss was less in the high mixture rate than in the low the loss of catechin most for 10 minutes of boiling, also as the concentration of AsA becomes higher but that of cathchin lower, and as that of AsA lower but that of catechin higher, the loss of catechin becomes less moreover in the case of the low content ratio of catechin, the stability of AsA was better in order of the pH 4, pH 5, pH 6, namely as the pH increased, but in the case of the high better in order of the pH 6, pH 5, pH 4, that is as in decreased.

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Response Characteristics of pH-ISFET Urea Sensor (pH-ISFET 요소센서의 감응특성)

  • Heung Lark Lee;Seung Tae Yang;Jong Hoon Yun;Chang Soo Kim;Byung Ki Sohn
    • Journal of the Korean Chemical Society
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    • v.36 no.6
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    • pp.864-871
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    • 1992
  • A preparation method and response characteristics of a urea sensor which consisted of pH-ISFET and urease-immobilized membrane were investigated. The pH-ISFET urea sensor was fabricated by immobilizing BSA and urease with glutaraldehyde on gate of the pH-ISFET. Effects of pH and concentration of working buffer and enzyme load on the pontentiometric response of the pH-ISFET urea sensor were examined. Response characteristics for the determination of urea in synthetic physiological saline solution (pH 7.4) were as follows. That is, the concentration range of linear response, slope of linear response (sensitivity), and response time were 5.0 ${\times}$ $10^{-4}$ ${\sim}$ 5.0 ${\times}$ $10^{-2}$M, 31.6 mV/decade and 4${\sim}$10 min, respectively.

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