• 제목/요약/키워드: pH standard test

검색결과 237건 처리시간 0.028초

Biochemical toxicity of Corexit 9500 dispersant on the gills, liver and kidney of juvenile Clarias gariepinus

  • Ugbomeh, A.P.;Bob-manuel, K.N.O.;Green, A.;Taylorharry, O.
    • Fisheries and Aquatic Sciences
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    • 제22권7호
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    • pp.15.1-15.8
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    • 2019
  • Corexit 9500 is a dispersant commercially available in Nigeria that is used to change the inherent chemical and physical properties of oil, thereby changing the oil's transport and fate with potential effects on the environment. The aim of this study was to assess the biochemical (enzymes and electrolyte) toxicity of Corexit 9500 dispersant on the gills, liver and kidney of juveniles of Clarias gariepinus after exposure for 21 days. One hundred sixty fish were used without gender consideration. Range-finding tests were conducted over a 96-h period after acclimatisation of the test organisms in the laboratory. The test organisms (10/treatment) were exposed to Corexit 9500 in the following concentrations-0.00, 0.0125, 0.025 and 0.05 ml/l in triplicate. Twenty-one days later, fish was dissected. 0.5 g from each of the following organs-gills, liver and kidney tissues-was removed, homogenised and tested for enzymes [superoxide dismutase (SOD), catalase (CAT), alanine aminotransferase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP)], urea, creatinine and electrolytes (sodium ($Na^+$), potassium ($K^+$), chloride ($Cl^-$), bicarbonate ($HCO_3{^-}$)) following standard methods. In the gills, SOD and ALT to AST ratio were significantly lower than in control while the creatinine was significantly higher in the toxicant. In the kidney, creatinine was significantly higher in fish exposed to the toxicant. In the liver, ALP increased in the toxicant while urea was decreased. The mean electrolyte concentrations ($Na^+$, $K^+$, $Cl^-$ and $HCO_3{^-}$) increased significantly in the concentration of the toxicant (P < 0.05). The alterations observed in the activities of these electrolytes and enzymes indicated that Corexit 9500 interfered with transamination and metabolic functions of the fish.

중소병원간호사의 근골격계증상 영향 요인 (Factors associate with Musculoskeletal Symptoms in Small and Medium Sized Hospital Nurses)

  • 정지수;은영
    • 근관절건강학회지
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    • 제24권3호
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    • pp.159-170
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    • 2017
  • Purpose: The purposes of this study were to identify musculoskeletal symptoms and to investigate factors associated with musculoskeletal symptoms in small and medium sized hospital nurses. Methods: The data were collected from 206 nurses working in Jeonnam. We used self administration questionnaires of KOSHA GUIDE H-9-2012 for musculoskeletal symptoms, PWI-SF for psychosocial stress, KOSS for job stress, and HPLP-II for health behaviors. The collected data were analyzed using the $x^2$, t-test, and logistic regression analysis in SPSS Ver. 23.0. Results: Sixty-six percent of the subjects had musculoskeletal symptoms. The shoulder had the highest symptom at 38.3%, followed by lower back at 32.5%, and neck at 29.1%. The mean and standard deviation score of psychosocial stress were $24.06{\pm}6.01$, those of job stress were $2.38{\pm}0.23$, and those of health behaviors were $2.14{\pm}0.39$. The items that were significantly different according to musculoskeletal symptoms were career ($x^2=6.67$, p<.036), one week overtime ($x^2=7.27$, p<.026), subjective health status ($x^2=4.29$, p<.038), and psychosocial stress ($x^2=7.99$, p<.010). In logistic regression analysis, career, and psychosocial stress were found to affect musculoskeltal symptoms. Conclusion: It is necessary to conduct preventive intervention to reduce musculoskeletal symptoms according to career and psychosocial stress for small and medium sized hospital nurses.

개량혼합토를 이용한 폐기물 매립지 차수층의 중금속 고정능력에 관한 연구 (A study on the fixation of heavy metals with modified soils in the landfill liner)

  • 노회정;이재영
    • 한국지하수토양환경학회지:지하수토양환경
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    • 제7권2호
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    • pp.63-71
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    • 2002
  • 본 연구에서는 점토차수재의 대체물질로서 매립지 현장토에 첨가재(시멘트, 벤토나이트 고화제)를 혼합하는 방법인 개량혼합토 공법을 대상으로 하여, 토목 환경적인 연구로서 차수/강도 효과 및 회분식/컬럼식 테스트를 통한 중금속의 고정능력에 관한 평가를 수행하였다. 지반공학적 연구(투수계수/압축실험)를 실시한 결과, CL 계열의 현장토(CL)에 첨가재(시멘트, 벤토나이트: 팽윤도로 구분하여 고품위 벤토나이트 B\circled1. 저품위 벤토나이트 B\circled2. 고화제)를 혼합함으로써 폐기물 매립지의 법적 기준(1x10-7cm/sec 이하)을 만족하였다. 또한 개량혼합토 제조시 시멘트 : 벤토나이트 : 고화제 = 90 : 60 : 1의 비율이 가장 적합하였으며, 팽윤도로 구별된 저품위 벤토나이트(B\circled2)의 사용가능성을 보여주었다. 첨가재의 종류에 따른 개량혼합토(column2, 3, 4)의 양이온교환능력(Cation Exchange Capacity, CEC) 측정 결과, 현장토와 비교하여 약 1.5배 정도 CEC가 증가한 것을 확인할 수 있었으나, 첨가재에 따른 큰 차이점은 보이지 않았다. 화학구성과 결정구조의 변화를 살펴보기 위한 XRF와 SEM측정 결과, 첨가재에 따른 고화토의 결정구조의 큰 변화 양상을 확인할 수 없었다. 컬럼을 사용하여 중금속($Pb^{2+}$ $Cu^{2+}$ , $Cd^{2+}$ , $Zn^{2+}$ l00mg/L)을 혼합한 인공침출수를 현장토(columnl)와 첨가재의 종류에 따른 개량혼합토(column2, 3, 4)에 적용시킨 결과, 현장토의 경우, 유출수의 pH가 감소됨과 동시에 $Cd^{2+}$$Zn^{2+}$가 유출되어 거의 파과점까지 도달하였으며, 현장토의 중금속 고정능력은 $Pb^{2+}$$Cu^{2+}$ > $Zn^{2+}$ > $Cd^{2+}$ 순으로 나타났다. 개량혼합토의 경우, 동일한 시점에서 column l에서 보여졌던 파과 현상은 나타나지 않았고, 팽윤도가 높은 B\circled1(column 2)보다 저급의 B\circled2(column 3)를 첨가한 개량혼합토가 화학적으로 훨씬 안정함을 보여주었으며, 시멘트, 벤토나이트와 함께 보조적으로 고화제(column 4)를 첨가했을 경우, 이러한 결과가 한층 더 두드러진다.

파래의 포자형성률을 이용한 해양생태독성시험 방법에 관한 연구 (Development of Marine Ecotoxicological Standard Methods for Ulva Sporulation Test)

  • 한태준;한영석;박경수;이승민
    • 한국해양학회지:바다
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    • 제13권2호
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    • pp.121-128
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    • 2008
  • 해조류를 이용한 수생태독성시험법으로 대형 녹조 구멍갈파래(Ulva pertusa)의 포자형성률을 endpoint로 사용하는 독성시험법이 개발되었다. 생태독성시험을 위한 최적 조건은 광조사량 $100\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 수소이온농도(pH) $7{\sim}9$, 염분 $25{\sim}35\;psu$ 그리고 수온 $15{\sim}20^{\circ}C$이다. 본 시험법의 민감도는 표준중금속(Cd, Cu, Pb, Zn)을 가지고 확인하였고, 오염 시료에 적용 가능성은 9 지역의 산업폐수 또는 생활하수 오니 용출액(elutriate)을 이용하여 이루어졌다. 네 종의 중금속에 대한 포자형성률 억제 반응의 $EC_{50}$ 값을 산출한 결과, 구리($0.062\;{mg}{\cdot}L^{-1}$) > 카드뮴($0.208\;mg{\cdot}L^{-1}$) >납($0.718\;mg{\cdot}L^{-1}$) > 아연($0.776\;mg{\cdot}L^{-1}$) 순으로 민감하게 나타났는데, 이러한 결과는 US EPA에서 제공하는 ECOTOX DB에 탑재되어 있는 국제적으로 공인된 수생태 독성시험법 결과와 비교해 볼 때, 더 높은 중금속 민감성을 보였다. 현장시료에 대한 포자형성률 억제 반응의 $EC_{50}$ 값을 살펴보면 산업폐수오니($EC_{50}=6.78%$)에서 가장 높고 정수장오니($EC_{50}=15.00%$)에서 가장 낮은 독성 반응을 보이는 것으로 나타났다. 산업폐수 또는 생활하수오니 용출액내에 함유된 독성원 농도와 산출된 $EC_{50}$ 값 사이에 상관성을 밝히기 위해 Spearman rank correlation test를 실시한 결과, 구리, 카드뮴, 납 그리고 아연이 구멍갈파래의 포자형성 저해 반응과 밀접한 상관관계가 있는 것으로 확인되었다. 본 시험법은 독성 민감성이 높고, 사용이 간편하고, 경제적이고, 해석이 용이하며, 대량의 시험재료 확보가 상시 가능하고, 배양이 어렵지 않아 매우 편리한 시스템이라고 할 수 있다. 특히, 파래의 포자형성 과정이 파래 집단의 성쇠와 밀접한 관련이 있으므로 생태적인 의미까지 포함하기에 보다 다양한 독성물질을 대상으로 독성민감성이 확인될 경우, 수서 생태독성을 진단하는데 유용한 프로토콜로 사용될 수 있을 것으로 사료된다. 또한, 파래류는 넓은 지리적 분포와 속 수준에서 포자형성 과정의 유사성 때문에 전 세계적으로 광역적 적용이 가능할 것으로 기대된다.

"감자에 대한 항생제(抗生劑) 피마리신의 통계적(統計的) 효과(效果) 분석(分析)" (INVESTIGATION OF THE EFFECT OF AN ANTIBIOTIC "P" ON POTATOES)

  • 김종훈
    • 품질경영학회지
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    • 제5권2호
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    • pp.59-120
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    • 1977
  • An antibiotic 'P', which is one of the products of the Gist Brocades N. V. is being tested by its research department as fungicide on seed-potatoes. For this testing they designed experiments, with two control groups, one competitor's product, eight formulations of the antibiotic to be tested in different concentrations and one mercury treatment which can not be used in practice. The treated potatoes were planted in three different regions, where bifferent conditions prevail. After several months the harvested potatoes are divided in groups according to their diameter, potato illness is analysed and counted. These data were summarised in percentage and given to us for Analysis. We approached and analysed the data by following methods: a. Computation of the mean and standard deviation of the percenage of good results in each size group and treatment. b. Computation of the experimental errors by substraction of each treatment mean from observed data. c. Description of the frequency table, plotting of a histogram and a normal curve on same graph to check normality. d. Test of normality paper and chi-sqeare test to check the goodness of fit to a normal curve. e. Test for homogeneity of variance in each treatment with the Cochran's test and Hartley's test. f. Analysis of Variance for testing the means by one way classifications. g. Drawing of graphs with upper and lower confidence limits to show the effect of different treatments. h. T-test and F-test to two Control mean and variance for making one control of Dunnett's test. i. Dunnett's Test and calculations for numerical comarision of different treatments wth one control. In region R, where the potatoes were planted, it was this year very dry and rather bad conditions to grow potatoes prevailed during the experimental period. The results of this investigation show us that treatment No.2, 3 and 4 are significantly different from other treatments and control groups (none treated, just like natural state). Treatment no.2 is the useless mercury formulation. So only No. 3 and 4, which have high concentrations of antibiotic 'P', gave a good effect to the potatoes. As well as the competitors product, middle and low concentrated formulations are not significantly different from control gro-ups of every size. In region w, where the potatoes got the same treatments as in region R, prevailed better weather conditions and was enough water obtainable from the lake. The results in this region showed that treatment No. 2, 3, 4, and 5 are Significantly different from other treatments and the control groups. Again No.2 is the mercury treatmentin this investigation. Not only high concentrated formulation of antibiotic 'P', but also the competitor's poroduct gave good results. But, the effect of 'P', was better than the competitors porduct. In region G, where the potatoes got the same treatments as in the regions R and w. and the climate conditions were equal to region R, the results showed that most of the treatments are not significantly different from the control groups. Only treatment no. 3 was a little bit different from the others. but not Significantly different. It seems to us that the difference between the results in the three regions was caused by certain conditions like, the nature of the soil the degres of moisture and hours of sunshine, but we are not sure of that. As a conclusion, we can say that antibiotic 'P' has a good effect on potatoes, but in most investigations a rather high concentration of 'P' was required in formulations.

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사질토양에서의 중금속의 지연효과와 이동성 (Retardation Effect and Mobility of a Heavy Metal in a Sandy Soil)

  • Kim, Dong-Ju;Baek, Doo-Sung
    • 대한지하수환경학회지
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    • 제5권3호
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    • pp.155-161
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    • 1998
  • 토립자 표면에서의 흡착에 의한 토양에서의 중금속의 지연효과는 잘 알려진 현상이다. 본 연구에서는 배치시험과 주상시험을 수행함으로써 사질 토양에서 Zn의 이동성에 대한 지연효과를 조사하였다. 주상시험은 파과곡선(BTC)으로 알려진 시간에 따른 용액의 농도를 측정함으로써 수행되었다. 추적자로는 10 g/L 농도의 NaCl과 ZnCl$_2$를 사용하였고, 각각의 용액을 토양시료의 상부경계에 순간주입한 후 정상류 상태에서 배출구로 빠져나온 용탈수의 농도를 EC-meter와 ICP-AES를 이용하여 측정하였다. 배치시험은 표준절차에 근거하여 이루어졌으며, 토양시료로부터 선별된 미세입자들을 다양한 초기농도의 ZnCl$_2$용액과 반응시켜 평형상태의 Zn 이온의 농도를 ICP를 이용하여 분석하였다. 주상시험의 결과는 i) ICP-AES에 의해 분석된 ZnCl$_2$의 첨두농도는 NaCl이나 총전기전도도로부터 구한 값보다 상당히 낮았고, ii) 두 종류의 추적자 모두 첨두농도의 도달시간은 상당히 일치하였다. 상대적으로 낮은 Zn의 농도는 Zn과 다른 양이온들간의 이온교환반응이 일어났고, 용탈수의 pH가 높은 값의 범위(7.0~7.9)에 있는 것으로 보아 Zn(OH)$_2$의 형태로 침전되었을 가능성이 있다는 것으로 설명될 수 있다. 첨두농도의 도달시간이 일치한다는 결과는 토양시료에서 지연효과가 일어나지 않았다는 것을 지시한다. Zn 이온의 뚜렷한 감소를 설명할 수 있는 유일한 방법은 CDE 모델에 용액상태에서 Zn 이온의 절대적 감소를 고려하는 감쇄계수(decay or sink coefficient)를 적용하는 것이었다.

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통나무 도구를 이용한 발목 펌프 운동이 수술실 간호사의 하지 부종 및 하지 통증에 미치는 효과 (A Study on the Effects of Ankle Pump Exercise in Reducing Lower Limbs Edema and Pain of Operating Room Nurses)

  • 배혜진;김주현
    • Journal of Korean Biological Nursing Science
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    • 제16권3호
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    • pp.235-243
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    • 2014
  • Purpose: The purpose of this study was to identify the effects of Ankle Pump Exercise on lower limb edema and pain of operating room nurses. Methods: This research was arranged on the basis of the nonequivalent control group pre-post test design. The participants were 41 nurses working in the operating room in 'S' hospital in Seoul and 'H' hospital in Guri City, Korea. Data were collected from August 20 to September 30, 2012. Edema of the lower extremities was measured in terms of the girths of ankles and calves. Pain of the lower extremities was measured in terms of VAS (Visual Analogue Scale) for pain. The data were analyzed by frequency, percentage mean, and standard deviation, using the chi2(p) and t-test, repeated measures ANOVA, and a paired t-test. The SPSS Win 20.0 program was used for analysis. Results: Hypothesis 1 - "There are significant differences in lower extremity edema between the experimental group and the control group." - was partially supported. Hypothesis 2 - "There are significant differences in lower extremity pain between the experimental group and the control group." - was supported. Conclusion: The results of this study demonstrate positive practical effects of the Ankle Pump Exercise in reducing the circumference of both ankles, reducing the circumferential edema of both calves, and alleviating the pain of nurses in the operating room. Based on these findings, application of the Ankle Pump Exercise can be considered as an effective method to prevent nurses' health problems in the lower extremities that can be induced by their work during surgical operations.

[InAs/GaSb] 응력 초격자에 기초한 [320×256]-FPA 적외선 열영상 모듈 제작 (Fabrication of [320×256]-FPA Infrared Thermographic Module Based on [InAs/GaSb] Strained-Layer Superlattice)

  • 이상준;노삼규;배수호;정한
    • 한국진공학회지
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    • 제20권1호
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    • pp.22-29
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    • 2011
  • InAs/GaSb 제2형 응력초격자(SLS)를 활성층에 탑재한 [$320{\times}256$] 초점면 배열(FPA) 적외선 열영상 모듈을 제작하고 열영상을 구현하였다. p-i-n형으로 설계된 소자의 활성층(i) 구조는 300 주기의 [13/7]-ML [InAs/GaSb]-SLS로 구성되어 있고, p와 n 전극층에는 각각 60주기의 [InAs:Be/GaSb]-SLS와 115 주기의 [InAs:Si/GaSb]-SLS 구조를 채용하였다. 시험소자의 광반응(PR) 스펙트럼으로부터 피크 파장(${\lambda}_p$)과 차단 파장(${\lambda}_{co}$)은 각각 ${\sim}3.1/2.7{\mu}m$${\sim}3.8{\mu}m$이고 180 K 온도까지 동작을 확인하였다. 단위 화소의 간격/메사는 $30/24{\mu}m$ 규격으로 설계되었으며, [$320{\times}256$]-FPA는 표준 광묘화법으로 제작하였다. $18/10{\mu}m$의 In-bump/UBM 공정과 flip-chip 결합 기술을 적용하여 FPA-ROIC 열영상 모듈을 완성하였으며, 중적외선용 영상구동 회로 및 S/W를 활용하여 열영상을 시연하였다.

임부의 성 태도와 성 만족과의 관계 (Pregnant Women's Attitude and Satisfaction for Sexuality)

  • 이영표;김신정;정금희
    • 대한간호학회지
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    • 제30권5호
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    • pp.1292-1302
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    • 2000
  • The relationship between the attitude and satisfaction for sexuality of pregnant women was observed to provide rationales of nursing intervention to help promote healthy sexual lifestyles. Questionnaires were collected from 211 obstetric outpatients of H university hospital in C city, Korea from February to May 1999. The research tools were D.S.F.I (Derogatis Sexual Function Inventory in Korean, Chronbach's $\alpha=$ 0.710) and Sexuality Satisfaction Method (Kim, 1997, Chronbach's $\alpha$ =0.864). Data was analyzed for frequency, mean, standard deviation, Pearson correlation, t-test and ANOVA by Windows SAS. The results of this study were as follows: Mean age of the subjects were 29.8; the average score of attitude to sex was moderate (27.60), and that of satisfaction sex was high (54.11); the positive relationship was shown between attitude and satisfaction for sexuality (r=0.51, p=.000); the higher educational and income levels of pregnant women and their husbands, were the better the attitude and satisfaction for was; professional women had better attitude than housewives. According to the results, it is suggested that the study is necessary to develop an effective nursing intervention related with the sexuality of pregnant women.

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의사와 간호사의 장애인에 대한 태도 (Attitudes of Physicians and Nurses toward with Disabled Person)

  • 정현경;김명희
    • 재활간호학회지
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    • 제7권2호
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    • pp.213-219
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    • 2004
  • Purpose: This study was designed to explore the attitudes of physicians and nurses. Method: Attitudes were measured by using the korean version of the SADP(Scale of Attitudes toward the Disabled Persons : Choi, G. H., & Lam, C. S., 2000). A korean version SADP consist of 24-item, six-point Likert-type scale(the points are -3, -2, -1, +1, +2, +3, where -3 is disagree very much and +3 is Agree very much), measuring general attitudes toward people with disabilities. The sample included 90 physicians, 114 nurses at a university hospital in Busan. The data were collected using questionnaires, and were analyzed using frequency, percentage, mean score, standard deviation, t-test and one-way ANOVA. Result: When studying the physicians group by itself, SADP scores tended to increase with women physicians, old age, married, higher educational level, more years of experience, medical unit. When studying the nurses group by itself, SADP scores tended to increase with the age of thirty, single, educational level, more years of experience, surgical unit. But not to a statistically significant degree. Conclusion: Findings suggest that the nurses had significantly higher score than the physicians(t=-4.63, p<.001). When studying the physicians group and the nurses group by itself, not to a statistically significant degree.

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