• Title/Summary/Keyword: pH paper

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Isolation of Anaerobic Cellulolytic Bacteria from the Rumen of Holstein Dairy Cows to Develop Feed Additives for Ruminants (반추동물용 사료첨가제개발을 위한 홀스타인 젖소의 반추위로부터 분리한 혐기성 섬유소 분해균의 특성연구)

  • Choi, Nag-Jin;Lee, Gi-Young;Jeong, Kwang-Hwa;Kim, Chang-Hyun
    • Korean Journal of Organic Agriculture
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    • v.20 no.3
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    • pp.327-343
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    • 2012
  • In order to develop a high cellulolytic direct-fed microorganism (DFM) for ruminant productivity improvement, this study isolated cellulolytic bacteria from the rumen of Holstein dairy cows, and compared their cellulolytic abilities via DM degradability, gas production and cellulolytic enzyme activities. Twenty six bacteria were isolated from colonies grown in Dehority's artificial (DA) medium with 2% agar and cultured in DA medium containing filter paper at $39^{\circ}C$ for 24h. 16s rDNA gene sequencing of four strains from isolated bacteria showed that H8, H20 and H25 strains identified as Ruminococcus flavefaciens, and H23 strain identified as Fibrobacter succinogenes. H20 strain had higher degradability of filter paper compared with others during the incubation. H8 (R. flavefaciens), H20 (R. flavefaciens), H23 (F. succinogenes), H25 (R. flavefaciens) and RF (R. flavefaciens sijpesteijn, ATCC 19208) were cultured in DA medium with filter paper as a single carbon source for 0, 1, 2, 3, 4 and 6 days without shaking at $39^{\circ}C$, respectively. Dry matter degradability rates of H20, H23 and H25 were relatively higher than those of H8 and RF since 2 d incubation. The cumulative gas production of isolated cellulolytic bacteria increased with incubation time. At every incubation time, the gas production was highest in H20 strain. The activities of carboxymethylcellulase (CMCase) and Avicelase in the culture supernatant were significantly higher in H20 strain compared with others at every incubation time (p<0.05). Therefore, although further researches are required, the present results suggest that H20 strain could be a candidate of DFM in animal feed due to high cellulolytic ability.

An Electrochemical Analysis on the Alloy Deposition of 80Ni-20Fe Permalloy Thin Film (80Ni-20Fe Permalloy 박모 동시석출 기구에 관한 전기화학적 고찰)

  • Lee, K. H.;Kang, Tak;Ra, H. Y.
    • Journal of the Korean institute of surface engineering
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    • v.15 no.1
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    • pp.11-18
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    • 1982
  • 80% Ni-Permalloy is soft magnetic material with high initial permeability and low magnetic coercive force Hc, and is used to computer memory cores and minirelays of communication e-ngineering. In this paper 80 Permalloy thin film on copper cathode was alloy-deposited from Watts so-lution contatining FeSO4$.$7H2O. The amount of FeSO4$.$7H2O in the solution, pH, temperature of the solution and plating current density were varied as parameters and the resulting comp-osition changes of deposited film were analyzed electrochemically with respect to the parame-ters. From the above procedure electroplating conditions for deposition of 80 Permalloy were est-ablished as following: 17-21 g/$\ell$ of FeSO4$.$7H2O in Watts solution, current density 1.0-2.0 Amp/dm2, pH 2.5-3.0 and temperature range of 50-60$^{\circ}C$.

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A study on performance analysis and merging techniques of sensors in water quality measurement (수질계측센서의 성능분석 및 센서 융합기술에 관한 연구)

  • Yang Keun-Ho;Yoo Byung-Kook
    • Journal of the Institute of Convergence Signal Processing
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    • v.7 no.3
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    • pp.143-148
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    • 2006
  • There are need to manage the water quality to supply an safety to consumer through pipe and reservoir the drinking water that produced in a clean water reservoir. However, a management of water quality and monitoring in reservoir have never been performed. Recently, the government has enforced standards of water quality. However, we have a inferior technology of water quality as compared with one in USA, Japan, Germany, etc. In case of water quality inspection and analysis equipments, sensor technology is very important to improve the water quality inspection and to develop the analysis equipments. In this paper, we analyze a law and a regulation for management of drinking water quality, and propose the measurement standards of drinking water quality in pH, conductivity, residual chloride, turbidity and water temperature. Then, we analyze electrode sensors that interference within pH, conductivity, residual chloride in interference experiment.

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Release of Ammonia Odor from AAFA (Ammonia Adsorbed Fly Ash) by Installation of NOx Reduction System

  • Kim, Jae-kwan;Park, Seok-un;Lee, Hyun-dong;Chi, Jun-wha
    • KEPCO Journal on Electric Power and Energy
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    • v.2 no.3
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    • pp.437-445
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    • 2016
  • This paper discussed the effect of ammonia concentration adsorbed on fly ash for the ammonia emission as AAFA (Ammonia Adsorbed Fly Ash) produced from coal fired plants due to operation of NOx reduction technologies was landfilled with distilled or sea water at closed and open systems, respectively. Ammonia bisulfate and sulfates adsorbed on fly ash is highly water soluble. The pH of ammonium bisulfate and sulfate solution had significant effect on ammonia odor emission. The effect of temperature on ammonia odor emission from mixture was less than pH, the rate of ammonia emission increased with increased temperature when the pH conditions were kept at constant. Since AAFA increases the pH of solution substantially, $NH_3$ in the ash can release the ammonia order unless it is present at low concentration. $NH_4{^+}$ ion is unstable in fly ash and water mixtures of high pH at open system, which is changed to nitrite or nitrate and then released as ammonia gas. The proper conditions for < 20 ppm of ammonia concentration released from the AAFAs landfilled in ash pond were explored using an open system with sea water. It was therefore proposed that optimal operation to collect AAFA of less than 168 ppm ammonia at the electrostatic precipitator were controlled to ammonia slip with less than 5 ppm at SCR/SNCR installations, and, ammonia odor released from mixture of fly ash of 168 ppm ammonia with sea water under open system has about 20 ppm.

Studies on the Naringinase Produced by Aspergillus nidulans (Part I) Purification and Characteristics of Naringinase (Aspergillus nidulans 가 생산하는 Naringinase 관한 연구 (제2보) Naringinase의 정제 및 물리화학적 성질)

  • 변유량;문순옥;유주현
    • Microbiology and Biotechnology Letters
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    • v.6 no.2
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    • pp.65-73
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    • 1978
  • Naringinase extracted from the culture media of Aspergillus nidulans was purified, and the activity was proved to be stronger by 781 fold in the part of precipitation with ammonium sulfate, and column chromatography using DEAE-Sephadex A-25 and Sephadex G-100. Two fractions which had the same enzyme activity were isolated by the purification. Both fractions showed the highest enzymes activity under the reaction conditions of pH 5.0 and 40$^{\circ}C$. Molecular weight of fraction I and fraction II were estimated as 78,000 and 26,000 respectively. This indicated that fraction I would be trimer of fraction II. The enzyme was inhibited by glucose and rhammose, and the Km value was calculated to be 2.3${\times}$ 10$\^$-6/ g/ml.

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Effect of pH in Hybrid Water Treatment Process of PVdF Nanofibers Spiral Wound Microfiltration and Granular Activated Carbon (PVdF 나노섬유 나권형 정밀여과와 입상 활성탄의 혼성 수처리 공정에서 pH의 영향)

  • Kyung, Kyu Myung;Park, Jin Yong
    • Membrane Journal
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    • v.25 no.4
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    • pp.358-366
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    • 2015
  • Flat membrane with $0.4{\mu}m$ pore size was prepared with PVdF (polyvinylidene fluoride) nanofiber, which has the advantages such as excellent strength, chemical resistance, nontoxic, non-combustibility. After that, spiral wound module was manufactured with it including a woven paper. Effect of pH was studied by comparing permeate fluxes and rejection rates of the spiral wound module using simulation solution including kaolin and humic acid. The recovery rate and filtration resistance were calculated after water back-washing at the end of filtration experiment. In addition, after the water filtrated by the spiral wound module was passed through a column filled with GAC (granular activated carbon), adsorption effect of GAC was investigated by measuring the turbidity and $UV_{254}$ absorbance.

Effects of Sweat on the Metal Frames of Eyeglasses (땀이 금속안경테에 미치는 영향)

  • Kim, So Ra;Choi, Myung Jin;Hwang, Jae Weon;Kim, Myung Soo;Chung, Seo Young;Park, Mijung
    • Journal of Korean Ophthalmic Optics Society
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    • v.20 no.1
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    • pp.9-14
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    • 2015
  • Purpose: In this paper, the changes in eyeglasses frames were investigated when the frames were exposed to artificial sweat. Methods: The changes in surface, hardness, and tensile strength of eyeglasses frames made of nickel silver, Monel, and titanium were tested after the exposure to the artificial sweat of pH 6.5, 6.0, and 5.0, during 1, 3, and 6 week(s), for each. Results: Corrosion pattern of eyeglasses frames made of nickel silver, Monel, and titanium was induced by artificial sweat, and the patterns were different from another frame materials. In proportion to the pH of artificial sweat and exposure time to artificial sweat, hardness of all frames made of three different material decreased, however, tensile strength of the three frames was not changed. Conclusions: The necessity of routine management for eyeglasses frames can be suggested since the changes in surface corrosion and hardness of eyeglasses frames induced by sweat were shown.

The Engineering and Environmental Properties of Reclaimed Concrete Materials as Road Materials (도로건설재료로 순환골재의 공학적·환경적 특성에 관한 기초연구)

  • Lee, Yong-Soo;Kwan, Yong-Wan;Hyun, Jae-Hyuk
    • Journal of the Korean GEO-environmental Society
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    • v.6 no.3
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    • pp.17-23
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    • 2005
  • In Korea, the production of reclaimed concrete materials has been increased due to the increase in the concrete structures taken down every year. The reclaimed concrete materials have been reused as road materials. However, the studies on their mechanical and environmental properties have been very limited. The recycled rate of the materials is currently low in Korea. This paper presents the investigation of mechanical and environmental properties of the reclaimed concrete materials, as well as the comparisons with those of gravel. For the evaluation of the mechanical and environmental characteristics, following tests were conducted on both reclamed materials and gravel; liquid limit, plasticity index, CBR, sand equivalent test, abrasion test, pH test, and column leaching test. The test results showed that the reclaimed concretes satisfy the requirements for use as roadbase, subbase, and subgrade materials, except base materials. The pH of reclaimed concrete materials was less than 11 and the leaching test results satisfied the regulatory requirement of Waste Management Act in Korea. Based on the investigations, it appears that the reclaimed concrete materials are environmentally safe and applicable for use as road materials.

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Inhibitory Action of Maillard Reaction Products Derived from Glucose Amino Acids on the Formation of N-nitrosamine (Glucose-아미노산계 Maillard 반응생성물의 니트로사민 생성억제작용)

  • 이동호;이태기;여생규;염동민;김선봉;박영호
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.1
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    • pp.137-142
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    • 1994
  • The present paper was carried out to investigate the inhibition of carcinogenic N-nitrosodimethylamine(NDMA) formation by Maillard reactiion products and nondialyzable melanoidins, obtiane dfrom the glucoseamino acids(Lys, Gly, Arg, His) model systems under different pH conditions(pH 1.2, 4.2 and 6.0). Maillard raction products and nondialyzable melanoidins, produced from the 4 model systems, had a inhibitory action of N-nitrosodimethylamine formation. The inhibitiondegree by the nondialyzable mealanoidins. at pH 1.2 was similar to that at pH 4.2 and that by ascorbic acid at pH 1.2 . Inhibitory action of N-nitrosodimehylamine formation by the reduced Maillard reaction products and nondialyzable melanoidins were lower than that of original samples. Accordingly, it is assumed that the inhibition of N-nitrosodimehtylamine formation of Maillard reaction products is due to their reducing powers.

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