• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.2
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• pp.216-224
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• 1997

In order to investigate the osmoregulation capability of grey mullet, Mugil cephalus with the different salinities, juvenile fish $(13.6{\pm}0.2\;TL)$ stocked in seawater (SW) were abruptly transferred to each experimental group $0\%SW(0\%_{\circ}),\;25\%SW(7.7\%_{\circ}),\;50\%SW(16.1\%_{\circ})\;and \;100\%SW(32.8\%_{\circ})$ and reared for 60 days. Blood samples were taken by the time schedule after the transfer. Plasma $Na^{+},\;K^{+},\;Cl^{-}$ and osmolality, muscle water content, and the electron microscopical observations of chloride cells were analyzed and made by the time schedule. In $100\%SW$, the maintainable levels of plasma $Na^{+},\;K^{+},\;Cl^{-}$ and osmolality were $167.1{\pm}7.7mM/l,\;9.1{\pm}2.1mM/l,\;137.8{\pm}5.6mM/l\;and\;351{\pm}18\;mOsm/kg$, respectively. These values were significantly changed at $6h\~1\;day$ after the beginning of the experiment with four different salinities. Fish from $0\%\;and\;25\%SW$ had lower osmolalities than those of fish from $50\%\;and\;100\%SW$, and showed the hyposmotic regulation pattern. At the end of the experiment (60 days after transfer), however, no significant difference was found in the concentrations of plasma $Na^{+},\;K^{+}\;and\;Cl^{-}$ among four experimental groups. Hematocrit was increased with salinity (P<0.01). After 10 days, fish from $0\%\;and\;25\%SW$ showed the hypertrophy, fusion and edema of epithelial layer in gill lamella. However, at the 15th day, epithelial layer in gill lamella was back to the normal status. On gill of fish from $0\%SW$, one apical pit held two or three chloride cells in common. Muscle water content was subsequently regulated to near the normal levels within 4 days, and there was no significant difference among four different salinities at the end of the experiment.

• The Journal of Korean Academy of Prosthodontics
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• v.47 no.1
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• pp.39-45
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• 2009

Statement of problem: Recently precalcification treatment has been studied to shorten the period of the implant. Purpose: This study was performed to evaluate the effect of precalcification treatment of $TiO_2$ Nanotube formed on Ti-6Al-4V Alloy. Material and methods: Specimens of $20{\times}10{\times}2\;mm$ in dimensions were polished sequentially from #220 to #1000 SiC paper, ultrasonically washed with acetone and distilled water for 5 min, and dried in an oven at $50^{\circ}C$ for 24 hours. The nanotubular layer was processed by electrochemical anodic oxidation in electrolytes containing 0.5 M $Na_2SO_4$ and 1.0 wt% NaF. Anodization was carried out using a regulated DC power supply (Kwangduck FA, Korea) at a potential of 20 V and current density of $30\;㎃/cm_2$ for 2 hours. Specimens were heat-treated at $600^{\circ}C$ for 2 hours to crystallize the amorphous $TiO_2$ nanotubes, and precalcified by soaking in $Na_2HPO_4$ solution for 24 hours and then in saturated $Ca(OH)_2$ solution for 5 hours. To evaluate the bioactivity of the precalcified $TiO_2$ nanotube layer, hydroxyapatite formation was investigated in a Hanks' balanced salts solution with pH 7.4 at $36.5^{\circ}C$ for 2 weeks. Results: Vertically oriented amorphous $TiO_2$ nanotubes of diameters 48.0 - 65.0 ㎚ were fabricated by anodizing treatment at 20 V for 2 hours in an 0.5 M $Na_2SO_4$ and 1.0 NaF solution. $TiO_2$ nanotubes were composed with strong anatase peak with presence of rutile peak after heat treatment at $600^{\circ}C$. The surface reactivity of $TiO_2$ nanotubes in SBF solution was enhanced by precalcification treatment in 0.5 M $Na_2HPO_4$ solution for 24 hours and then in saturated $Ca(OH)_2$ solution for 5 hours. The immersion in Hank's solution for 2 weeks showed that the intensity of $TiO_2$ rutile peak increased but the surface reactivity decreased by heat treatment at $600^{\circ}C$. Conclusion: This study shows that the precalcified treatment of $TiO_2$ Nanotube formed on Ti-6Al-4V Alloy enhances the surface reactivity.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.5
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• pp.323-332
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• 2003

This study was carried out to provide information for the present status of soil pollution near abandoned old-zinc mining area through analysis of bound form and 0.1 N-HCl extractable concentrations of heavy metals in soils and plants. Feasibility of endemic plants for phytoremediation was evaluated by the investigation of vegetation in soils. Cd contents of the selected samples near old-zinc mining soils ranged from 0.2 to $42mg\;kg^{-1}$. Nonagricultural soils near the mining area contained great amounts of Zn, Pb, Cd, and Cu than the paddy and upland soils. Some Korean wild plants, Artemisia princeps, Artemisia montana, Erigeron canadensis, and Pueraria thunbergiana, were found to grow vigorously in the studied area. Among them, Artemisia princeps was selected as a possible phytoremediator for cleaning heavy metal contaminated soils. Artemisia princeps contained about 43 and $52mg\;kg^{-1}$ of Cd in their root and shoot as dry weight, respectively. Average contents of Cd in the rhizosphere soil, $15.68mg\;kg^{-1}$, was slightly higher than the soil-root interface soils, $14.1mg\;kg^{-1}$. Sequential extraction of Cd contaminated soils showed that average $2.4mg\;kg^{-1}$ (about 7%) of cadmium existed as exchangeable form and the average amounts increased as follows : adsorbed < organically bound < exchangeable << oxide carbonate << sulfide residual fractions. Amendment of organic by-product fertilizer in metal-contaminated soils promoted the growth of roots significantly as compared with the other treatments containing chemical fertilizer.

• Journal of the Korean Applied Science and Technology
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• v.36 no.3
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• pp.1018-1027
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• 2019

Natural-derived protein-derived low molecular weight peptides have been known to have physiological activities such as antioxidant, hypertension relief, immunomodulation, pain relief and antimicrobial activity. In this study, the low-molecular peptides were produced using commercial proteases (alcalase, bromelain, flavourzyme, neutrase, papain, protamex), and the antioxidant activity (DPPH scavenging activity, superoxide radical scavenging activity, hydroxy radical scavenging activity, and metals chelation capacity), constituent amino acid and molecular weight of the peptide were analyzed. Enzyme reaction was performed by adding 50 g of chopped Ogae meat slurry and 2%(w/v) protein enzyme into the enzyme reactor for 2 h at a pH of 6 and a temperature of $60^{\circ}C$. The degree of hydrolysis(%) after the reaction ranged from $36.65{\pm}4.10%$ to $70.75{\pm}5.29%$. The highest degree of hydrolysis of protamex was 46.3%, and the highest value of papain hydrolysate was $70.75{\pm}5.29%$. On the other hand, alcalase hydrolysate showed the lowest value of $36.65{\pm}4.10%$. Bromelain-treated low molecular weight peptides showed the highest DPPH radical scavenging activity and the lowest scavenging activity of alcalase-treated peptides. Superoxide radical scavenging activity showed that bromelain treated low molecular peptide showed the highest radical scavenging activity of 50% or more. Hydroxyl radical scavenging activity ranged from about 16.73 to 69.16%, the highest among bromelain-treated low molecular peptides. $Fe^{2+}$ chelation abilities showed a distribution between about 17.85 to 47.84%. The chelation capacity of the hydrolysates was not significantly different without any difference to the enzymes used. The results of amino acid analysis showed differences between hydrolysates of alcalase, bromelain, flavourzyme, neutrase, papain, and protamex enzymes. The most amino acid was glutamic acid. The molecular weight distribution of the enzyme hydrolyzates was in the range of 300-2,000 Da, although the molecular weight distribution differed according to the treated enzymes.

• Journal of Practical Agriculture & Fisheries Research
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• v.22 no.2
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• pp.69-79
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• 2020

In this study, the anti-inflammatory and cytotoxic effects of hot-water extracts from 10 kinds of macroalgae in Korea were investigated. It was selected materials in consideration of biological activity and industrial potential as follows: Caulerpa okamurae; Codium fragile; Ulva australis; Ishige foliacea; Saccharina japonica; Sargassum horneri; Undaria pinnatifida; Gloiopeltis tenax; Gracilaria verrucosa; Porphyra tenera. Results showed that S. japonica and G. tenax significantly decreased NO productionn in LPS-stimulated Raw 264.7 cells at concentrations of 100, 1000 ㎍/mL and 1000 ㎍/mL, respectively. However, most of the other macroalgae used in the experiment did not affect NO production. It was observed that all macroalgae extracts except for the highest concentration (1000 ㎍/mL) treatment group of P. tenera did not affect the viability in Raw 264.7 cells. In addition, there was not significant decrease in cell viability by macroalgae extracts treatment in HINAE cells. These results suggest that S. japonica and G. tenax could be used as potential safe natural anti-inflammatory agents for food and feed additives. Also, the results of this study are expected to be used as basic data for the development of functional materials for 10 kinds of macroalgae resources in Korea.

• Journal of Bio-Environment Control
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• v.22 no.4
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• pp.392-399
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• 2013

This study aimed to investigate responses of photosynthesis, plant growth, and phytochemical contents to different artificial light sources for 'Seneca RZ' and 'Gaugin RZ' two butterhead lettuce (Lactuca sativa L.). In this study, fluorescent lamps (FL), three colors LEDs (red, blue and white, 5 : 4 : 1; RBW) and metalhalide lamps (MH) were used as artificial lighting sources. Photoperiod, air temperature, relative humidity, EC, and pH in a cultivation system were maintained at 16/8 h, $25/15^{\circ}C$, 60~70%, $1.4{\pm}0.2dS{\cdot}m^{-1}$, and $6.0{\pm}0.5$, respectively. The photosynthetic rate of both two butterhead lettuce were the highest under RBW in middle growth stage. However, in late growth stage, the photosynthetic rate of both two butterhead lettuce were higher under RBW and MH than FL. The light sources showed significant results for plant growth but those effects were different to variety. Fresh and dry weight of 'Gaugin RZ' butterhead lettuce under MH were heavier than other lights in all growth stages. Growth of 'Seneca RZ' butterhead lettuce was maximized highest under MH in middle growth stage and FL in late growth stage. In the leaf tissue of 'Seneca RZ' butterhead lettuce, tipburn symptom occurred under all light sources and in the leaf tissue of 'Gaugin RZ' butterhead lettuce, it occurred under two light sources except for fluorescent lamps in late growth stage. kinds of lamp affect plant growth more than plant quality. Relative growth rate of both two butterhead lettuce was faster in middle growth stage than late stage. Growth of 'Gaugin RZ' was shown by kinds of lamp in middle growth stage and but it was not significantly affected by light sources and variety in late stage. Most of the phytochemical contents of two butterhead lettuce were significantly affected by different light sources. Contents of all vitamins showed higher than other light sources on RBW for both two lettuce, especially ${\beta}$-Carotene content of 'Gaugin RZ' was the highest. Plant growth, photosynthesis, and phytochemical contents were observed significant effects by different light sources for two butterhead lettuce but those effects were highly different between variety and kinds of phytochemicals. Therefore, the selection of optimum light source should be considered by variety and kinds of phytochemicals in the plant factory.

• Horticultural Science & Technology
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• v.30 no.3
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• pp.250-255
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• 2012

The effect of different source silicon ($CaSiO_3$, $K_2SiO_3$, and $NaSiO_3$) and their application methods (foliar application and subirrigation) on the growth of potted kalanchoe was investigated. Rooted terminal cuttings of Kalanchoe blossfeldiana 'Peperu' were transplanted into 10.5 cm plastic pots containing a commercial growing medium. Then, a nutrient solution, containing 0 or $50mg{\cdot}L^{-1}$ Si as $K_2SiO_3$, $Na_2SiO_3$, or $CaSiO_3$ and adjusted to EC 1.4-$1.6mS{\cdot}cm^{-1}$ and pH 6.0, was supplied through subirrigation along with the nutrient solution or by a foliar application. Plants were grown in a glasshouse under a mean temperature of $23^{\circ}C$ and RH of 70-80%. After 12 weeks of cultivation, plant growth characteristics and leaf tissue contents of P, K, Ca, Mg, Na, S, and Si were measured. Both subirrigational supply and foliar application of Si decreased the plant height and flower stem length. However, the plant condition in the foliar application resulted in disease-like soft rot on the leaf. Among three silicon sources tested, $CaSiO_3$ supplied through a subirrigation system increased shoot tissue contents of Si and chlorophyll as compared to the $Na_2SiO_3$ or $K_2SiO_3$ treatment. Shoot tissue contents of Ca, K, and Na increased when the plant was supplied with $CaSiO_3$, $K_2SiO_3$, and $Na_2SiO_3$, respectively. Subirrigational supply of $K_2SiO_3$ and $NaSiO_3$ decreased the shoot tissue contents of Ca and Mg, and K and Ca, respectively. Therefore, $CaSiO_3$ supplied through a subirrigation system could improve plant quality of kalanchoe 'Peperu' making compact potted plants.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.38 no.2
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• pp.95-107
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• 2020

The purpose of this study is to provide the basic data by analyzing and analysing the Growth Conditions of old Trees in Yangdong village. This study investigated about the conducted on tree information, soil information, and tree health. The result are as follows. The trees information in Yangdong Village consists of Juniperus chinensis, Salix chaenomeloides, Salix pseudolasiogyne, Celtis sinensis, Zelkova serrata, Gleditsia japonica, and Gleditsia sinensis trees, The range of height was 4.0~17.0m, and the diameter was 0.51~1.34m, Juniperus chinensis trees of No.17 was most large. In the results of soil analysis, there showed that acidity was pH4.1~6.3, hardness of 5~48mm, organic matter content of 21.2~29.1g/kg, electrical conductivity(EC) of 0.34~1.76dS/m, available P₂O₅ of 79.8~451.6mg/kg, exchangeable K of 0.22~1.71cmol+/kg, exchangeable Ca of 4.98~7.44cmol+/kg, exchangeable Mg of 0.67~2.19cmol+/kg, exchangeable Na of 0.19~1.04cmol+/kg and cation exchange capacity(C.E.C) of 7.23~13.02cmol+/kg. As a result, the highest number of tree health levels is 8 of 11trees of Celtis sinensis, 2 of 7trees of Zelkova serrata, and 3 of Gleditsia sinensis, and 13 of 30 trees of health levels, The Older trees with high infection, spoil and hollowed part were the remaining trees except for the healthy part. Relatively, more than half of the number of targets is infected, decay, and the hollowed site, and it is necessary to perform surgery on the damaged area. In addition, preservation and protection measures should be implemented by supplying root nutrients for trees, controlling nutrients in the body to prevent secondary and tertiary damages that cause the infection site to metastasize to the health site, In order to continually monitor the trees, measures to improve the location environment and management of the trees should be sought.

• Clinical and Experimental Reproductive Medicine
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• v.33 no.3
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• pp.189-198
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• 2006

Objective: We previously described that Diva is highly expressed in matured metaphase II (MII) oocytes compared to immature germinal vesicle (GV) oocytes in mouse. We report here that the expression of Diva transcript as well as protein is oocyte-specific. To elucidate its physiological role in oocyte, the binding partner(s) of Diva has been identified by using immunoprecipitation (IP) followed by Mass Spectrometry. Methods: NIH/3T3 cells were transiently transfected for 24 h with either empty vector for control or FLAG-tagged mouse Diva construct, and IP was performed with anti-FLAG antibody. The immuno-isolated complexes were resolved by SDS-PAGE on a 12% gel followed by Coomassie Blue staining. For in-gel digestion, 15 bands of interest were excised manually and digested with trypsin. All mass spectra were acquired at a positive reflector mode by a 4700 Proteomics Analyzer (Applied Biosystems, Framingham, MA). Proteins were identified by searching the NCBI nonredundant database using MASCOT Peptide Mass Fingerprint software (Matrixscience, London). Results: Diva-associated complexes were formed in FLAG-tagged mouse Diva-overexpressed NIH/3T3 cells via IP using anti-FLAG-conjugated beads. Among the excised 15 bands, actin and actin-binding proteins such as tropomyosin, tropomodulin 3, and ${\alpha}$-actinin were identified. Binding between Diva and actin or tropomyosin was confirmed by IP followed by Western blot analysis. Both bindings were also detected endogenously in mouse ovaries, indicating that Diva works with actin and tropomyosin. Conclusions: This is the first report that immuno-isolated Diva-associated complexes are related to actin filament of the cytoskeletal system. When we consider the association of Diva with actin and tropomyosin, oocyte-specific Diva may play a role in modulating the cytoskeletal system during oocyte maturation.

• Journal of Bio-Environment Control
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• v.28 no.4
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• pp.447-453
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• 2019

In this study, the effect of supplying volume and frequency of a nutrient solution consisted with $NO_3-N$ 4.6, $NH_4-N$ 3.4, $PO_4-P$ 3, K 3, Ca 4.6 and Mg $2.2mmol{\cdot}L^{-1}$ on growth and fruit quality of 'Duke' blueberry was investigated. Three years old 'Duke' blueberry bushes cultivated in containers ($60{\times}80{\times}40cm$) filled with 130L peat moss and 40L pearlite (v/v) were selected for the experiment. The growth containers were mulched with sawdust. Two different volumes (4L and 8L) of nutrient solution was tested at three different supplying frequencies (one time, two times, and three times) per week and the drainage quality of nutrient solution and fruit quality of 'Duke' blueberry was evaluated. The optimal drainage rate for the vegetable cultivation is known to be 20-30%. The results revealed that the average drainage rates of 27% and 29% for the nutrient solution supplied in 'Duke' blueberry growth medium at 4L, 2 times/7 days and 4L, 3 times/7days, respectively. The highest shoot diameter (4.2mm) and shoot length (31cm) of 'Duke' blueberry was recorded with the 8L of nutrient solution supplied at 3 times per 7 days. According to the analysis of inorganic components in the drainage of nutrient solution, there was a tendency of absorbing nitrogen at the early stage of growth. The supplying volume and frequency of nutrient solution was not significantly affected on 'Duke' blueberry fruit weight, soluble solids content, and titratable acidity. The highest yield per bush (2.7kg) was recorded for the nutrient solution supplied with 4L at three times per 7 days, while the 4L nutrient solution supplied at one time per 7 days resulted the lowest yield of 1.4kg per bush. Consequently, the tested nutrient solution can be applied for the 'Duke' blueberry bushes with the volume of 4L at three times per week for the better crop growth.