• Journal of Animal Science and Technology
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• 제50권2호
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• pp.209-216
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• 2008

본 연구는 꽃사슴에게 고농도의 셀레늄을 급여하였을 때 꽃사슴의 혈액, 녹용 및 생리반응에 미치는 영향을 평가하고자 실시하였다. 3두의 꽃사슴에게 고농도 셀레늄을 급여하였을 때의 혈액 내의 셀레늄 농도와 셀레늄 흡수율 및 축적률을 조사하고, 혈액 내 스트레스 관련호르몬 및 biochemical parameters를 조사하였다. 8두의 수컷 꽃사슴을 공시하여 셀레늄을 첨가하지 않은 사료를 급여한 구와 셀레늄을 사료 kg당 6mg을 첨가한 사료를 20일간 급여한 구로 나누어 생산한 녹용의 셀레늄 함량을 조사하였다. 꽃사슴에게 고농도 셀레늄 급여는 급여 30일 이후에 혈액 중의 셀레늄 농도를 유의하게 증가시켰고(p<0.05), 축적률은 59.15%를 나타내었다.전 실험 기간 동안 고농도 셀레늄의 급여로 인한 스트레스 관련 호르몬 및 biochemical parameters의 변화는 나타나지 않았다. 녹용 내 셀레늄 함량은 대조구의 경우 상대 0.11ppm, 중대 0.08ppm, 하대에 0.08ppm을 셀레늄 급여구에서는 상대 0.45ppm(p<0.001), 중대 0.21ppm(p<0.01), 하대에 0.15ppm(p<0.05)으로 셀레늄 급여로 인하여 유의하게 증가하였으며, 상대에서의 셀레늄 함량이 가장 높았다(p< 0.05).이상의 결과로부터 꽃사슴에게 고농도의 셀레늄을 급여할 경우 녹용내로 다량의 셀레늄을 전이시킬 수 있는 것으로 판단되었고, 40일까지 고농도의 셀레늄 6mg/kg 농도 급여에 있어 생리적 중독 증상은 없었으나 혈중 소실정도를 감안해 볼 때 20일간 급여하는 것이 적합하다고 사료된다. 향후 셀레늄 강화 녹용 생산을 위해 급여하는 셀레늄의 최적 농도와 급여 기간에 대한 연구가 더 필요할 것으로 사료된다.

• 한국축산식품학회지
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• 제37권5호
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• pp.773-779
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• 2017

The effect of addition of the probiotic Bifidobacterium longum KACC 91563 on the chemical and sensory properties of Kwark cheese produced using CHN-11 as a cheese starter were investigated. The addition of B. longum KACC 91563 to Kwark cheese did not change the composition or pH value of the cheese, compared with control. B. longum KACC 91563 survived at a level of 7.58 Log CFU/g and did not have any negative effect on survival of the cheese starter. A sensory panel commented that the addition of B. longum KACC 91563 made Kwark cheese more desirable to consumers, and that the probiotic supplementation had no effect on perceived taste. Thus, B. longum KACC 91563 can be used for inclusion of probiotic bacteria in cheese.

• Biomolecules & Therapeutics
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• 제11권3호
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• pp.169-173
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• 2003

Alkylthioureido-1,3-propandiols (KY353X series) were synthesized and evaluated as inhibitors for neutral sphingomyelinase (N-SMase). To examine whether KY353X series inhibit N-SMase, we purified the N-SMase from bovine brain. The N-SMase was partially purified by sequential chromatographies of DEAE-Cellulose anionic exchange and phenyl-5PW hydrophobic HPLC. These seqeuntial procedures for N-SMase resulted in a 67-fold purification and excluded other isoforms of SMase. Based on in vitro assay, KY353X series inhibited N-SMase activity in time, concentration-dependent manners and completely inactivated N-SMase at 50 $\mu$M. In particular, KY3535 and KY3536 inhibited more effectively than the others. To further determine the .inhibitory pattern, a Dixon plot was constructed, to showing that the inhibition by KY3535 and KY3536 were competitive. The inhibition constant (Ki) of KY3535 and KY3536 was 1.7 $\mu$M and 2.5$\mu$M in 100 mM Tris-HCl buffer, pH 7.0, respectively.

• Asian Pacific Journal of Cancer Prevention
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• 제15권14호
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• pp.5673-5679
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• 2014

Background: Previous published data on the association between CYP1A2 rs762551, rs2069514, rs2069526, and rs2470890 polymorphisms and lung cancer risk have not allowed a definite conclusion. The present meta-analysis of the literature was performed to derive a more precise estimation of the relationship. Materials and Methods: 8 publications covering 23 studies were selected for this meta-analysis, including 1,665 cases and 2,383 controls for CYP1A2 rs762551 (from 8 studies), 1,456 cases and 1,792 controls for CYP1A2 rs2069514 (from 7 studies), 657 cases and 984 controls for CYP1A2 rs2069526 (from 5 studies) and 691 cases and 968 controls for CYP1A2 rs2470890 (from 3 studies). Results: When all the eligible studies were pooled into the meta-analysis for the CYP1A2 rs762551 polymorphism, significantly increased lung cancer risk was observed in the dominant model (OR=1.21, 95 % CI=1.00-1.46). In the subgroup analysis by ethnicity, significantly increased risk of lung cancer was observed in Caucasians (dominant model: OR=1.29, 95%CI=1.11-1.51; recessive model: OR=1.33, 95%CI=1.01-1.75; additive model: OR=1.49, 95%CI=1.12-1.98). There was no evidence of significant association between lung cancer risk and CYP1A2 rs2069514, s2470890, and rs2069526 polymorphisms. Conclusions: In summary, this meta-analysis indicates that the CYP1A2 rs762551 polymorphism is linked to an increased lung cancer risk in Caucasians. Moreover, our work also points out the importance of new studies for rs2069514 associations in lung cancer, where at least some of the covariates responsible for heterogeneity could be controlled, to obtain a more conclusive understanding about the function of the rs2069514 polymorphism in lung cancer development.

• 대한약침학회지
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• 제9권2호
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• pp.129-134
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• 2006

Objectives : The purpose of this study was to investigate the clinical progress of patients diagnosed as sequela of traffic accident. Methods : We studied 34 cases who were admitted in Semyung University Oriental Hospital from 1st, January, 2005 to the 31st, December, 2005. And we came to some conclusion about clinical tendencies as follows. Results and Conclusions : 1. Women had occupied more than men, and 14 persons in 20th were the most distribution of age. 2. In distribution of onset, less than 7 days was the most(58.8 %), followed by less then 28 days(20.6 %). 3. Most patients used western medical hospital(47.1 %) and oriental medical hospital(44.1 %) as primary hospital. 4. In chief complaint, cervical pain was most(32 cases, 94.1%), the others were low back pain(22 cases, 64.7 %), back pain(5 cases, 14.7 %), chest pain(5 cases, 14.7 %). 5. In treatment methods, acupuncture and physical therapy was most(33 cases, 97.1%), the others were Extract-med.(31 cases, 94.0%), electric-acupuncture(27 cases, 79.4%), Herb-med.(23 cases, 67.6%), Herb-acupuncture(l8 cases, 52.9%) and Taping therapy(15 cases, 44.1%). 6. In hospital days, $8{\sim}14$days was most(l5 cases, 44.1%), the other were 15-21days(l2 cases, 35.3%). over the 29 days(4 cases, 11.8%) and 22-28 days(3 cases, 8.8%). 7. 34 patients were examined, and all cases were 'improved'. And statistically significance compared after-treatment with before-treatment group(p<0.005).

• 한국산업보건학회지
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• 제20권3호
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• pp.192-202
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• 2010

This study aimed to suggest the severity of indoor air pollutants in screen gold arenas which were not sufficiently investigated in Korea up to now and to help users to enjoy golf in more pleasant indoor environment. The indoor environment survey was conducted with 21 screen gold arenas in Seoul from Oct. 28, 2008 to March 13, 2009. Indoor air quality was measured and analyzed in accordance with the Air Pollution Process Test Method specified bu NIOSH(2005). The screen golf arenas are mostly in the underground floors in this study, 4 on the ground floors(19.0%) and 17 in the underground floors(81.0%). In the air in screen golf arenas, the geometric mean of benzene, toluene, ethylbenzene and xylene were 2.92 ${\mu}g/m^3$, 70.34 ${\mu}g/m^3$, 14.00${\mu}g/m^3$ and 31.43 ${\mu}g/m^3$, respectively, which exceeded the exposure limites. Each arena exceeded the exposure limit for one pollutant each. However, styrene didn't exceed the limit as 8.09 ${\mu}g/m^3$. Furthermore, the geometric mean of formaldehyde was 63.11${\mu}g/m^3$ and 7 arenas exceeded the limit. The geometric mean of volatile organic compounds(VOCs) was 428.41${\mu}g/m^3$ and 10 arenas exceeded the limit. For the density distribution of pollutants by location, benzene, toluene, ethylbenzene, xylene, styrene and formaldehyde showed higher density distribution in underground spaces, for which the statistically significant difference was not found. However, PM10 showed the statistically significant difference (p<0.05). In accordance with the analysis on the correlation between the density of pollutants in the screen golf arenas, Pearson correlation coefficient between ethylbenzene and styrene was 0.980, very significant correlation(p<0.01). The correlation coefficients between the density of toluene, ethylbenzene, xylene and styrene and that of VOCs were 0.543, 0.434, 0.451 and 0.459, respectively, which demonstrated the statistically significant difference (p<0.05).

• Asian Pacific Journal of Cancer Prevention
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• 제15권14호
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• pp.5895-5900
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• 2014

Background: Metabolic syndrome (MS) is a cluster of complicated disorders caused by the interactive influencing factors of heredity and environment, which predisposes to many cnacers. Results from epidemic research indicate that stress is tightly related to the pathogenesis of MS and neoplasia. This paper aims to investigate the association between psychological stress and MS with respect to the tumor necrosis factor alpha (TNF${\alpha}$) and neuropeptide Y (NPY) genes in the Han and Hui ethnic groups. Methods: All subjects for this case-control study matched strict enrollment criteria (nationality, gender and age) and lived in the city of Wu Zhong of Ningxia Province in China. The enrolled group contained 102 matched pairs of Hui ethnic individuals and 98 matched pairs of Han ethnic individuals. Enrolled subjects completed the general Symptom Checklist-90 (SCL-90). The TNF${\alpha}$-308G/A variant and NPYrs16147 polymorphism were detected in case (81 males, 119 females) and control (81 males, 119 females) groups by polymerase chain reaction (PCR) amplification. Results: Nine factors of the SCL-90 were found to be statistically different (p<0.05) between case and control groups. The homozygous mutant genotype (AA) and the mutant allele (A) of the TNF${\alpha}$-308G/A gene were less frequently observed in the control population compared to the case group. The odds ratio (95% confidence interval) in "Allele" for MS was 2.28 (1.47-3.53), p=0.0001, while "OR" was 1.11 (0.83-1.47), p=0.15, for the NPYrs16147 gene polymorphism. Conclusions: Psychological stress has been positively associated with MS. A previous study from our group suggested there were differences in the level of psychological stress between Hui and Han ethnic groups. Furthermore, we found that the stress-related TNF${\alpha}$ gene was associated with MS for both Han and Hui ethnic groups. In contrast, NPY may be a possible contributor to MS and associated cancer for the Han ethnic group.

• 대한한의학회지
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• 제31권2호
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• pp.48-63
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• 2010

Objectives: Yonnyuniksoogobon-dan (Yan Nian Yi Shou Gu Ben Dan 延年益壽固本丹) is composed of 11 herbs (Polygoni Mutiflori Radix, Lycii Radicis Cortex, Polia, Rehmanniae Radix, Rehmanniae Radix Preparat, Asparagi Radix, Liriopis Tuber, Lycii Fructus, Acori Graminei Rhizoma, Angelicae Acutiloba Radix, and Pini Folium) based on Yonryunggobon-dan (Yan Ling Gu Ben Dan 延齡固本丹) and Yonnyuniksoobulrho-dan (Yan Nian Yi Shou Bu Lao Dan 延年益壽不老丹). This study evaluated hair growth promoting effect of Yonnyuniksoogobon-dan on the shaved C57BL/6 mice. Methods: Yonnyuniksoogobon-dan was treated by oral administration (Sample I) and oral administration plus skin application (Sample II) once a day for 12 days. Hair regrowth was photographically and histologically determined during the experimental period. Hair growth cycle related factors (EGF, TGF-${\beta}1$) and vascular factors (VEGF, iNOS) were also determined with immunohistochemistry. Results: 1. On gross observation of hair regrowth, Sample I and Sample II groups demonstrated acceleration of hair regrowth. 2. The hair regrowth index of the Sample I group increased significantly from 7 days (P<0.05) to 12 days (P<0.01) after the shave while that of the Sample II group significantly increased at 12 days (P<0.05). 3. On histological observation, both Sample I and Sample II groups demonstrated histological improvement and increases of number and diameter of the hair follicles. 4. EGF expressions on the root sheath of hair follicles were up-regulated in both Sample I and Sample II groups. 5. TGF-${\beta}1$ expressions on the root sheath of hair follicles were not regulated in Sample I or Sample II groups. 6. VEGF expressions in the surrounding tissues of hair follicles were up-regulated in both Sample I and Sample II groups. 7. iNOS expressions in the surrounding tissues of hair follicles were down-regulated in both Sample I and Sample II groups. Conclusions: These results suggest that Yonnyuniksoogobon-dan has hair growth-promoting activity and these effects relate to up-regulations of EGF and VEFG expressions and down-regulations of TGF-${\beta}1$ and iNOS expressions on hair roots.

• 한국방사선학회논문지
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• 제18권4호
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• pp.345-353
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• 2024

본 연구의 목적은 디지털 방사선 장비를 이용하여 요추 측면 검사 시 사용되고 있는 조사야 크기 및 조사야 크기에 영향을 미치는 요인의 상관관계 분석을 통해, 적절한 조사야 크기 및 중심 엑스선 입사지점을 제안하는데 있다. 2023년 1월 1일부터 2023년 12월 31일까지 S병원 영상의학과에서 요추 측면 검사를 시행한 환자 중 본 연구 목적에 적합한 148명의 검사 결과를 분석하였다. 측정 방법은 영상에서 나타난 전체 가로 폭을 검사 시 사용된 조사야 가로폭의 크기(CS)로 설정하고, 영상에 포함된 등쪽 조사야 끝에서부터 제 3요추의 척추 돌기관절까지 수직으로 연결한 거리(AJD), 영상의 조사야 등쪽 끝 부분에서 제 3요추 몸통 중심까지의 거리(BD), 영상의 등쪽 조사야 끝 부분에서 제 3요추의 척추 경 중심까지의 거리(PD)를 측정하였다. 성별, 연령, 신장, 체중, 체질량지수에 따른 종속변수의 평균값의 비교분석을 위해 독립표본 t 검정과 일원배치분산분석을 이용해 평균값을 비교하였으며, 사후분석으로는 duncan을 이용했다. 독립변수와 종속변수 간의 상관성은 pearson 상관분석을 통해 분석하였다. 본 연구에서 통계적 유의성은 p값 0.05 이하를 유의한 것으로 설정하였다. 요추 측면 검사 시 CS의 평균값은 252.45 mm 였으며, AJD는 102.11 mm, BD는 141.17 mm, PD는 119.73 mm로 나타났다. CS 및 AJD, BD, PD는 남성이 여성에 비해 평균값이 크게 나타났으나, 성별에 따른 평균값 차이에 대한 통계적 유의성은 BD에서만 나타났다(p<0.05). 연령에 따른 그룹별 평균값에 약간의 차이는 있었지만 통계적 유의성은 없었다(p>0.05). 신장, 체중, 체질량지수에 따른 CS 및 AJD, BD, PD의 평균값은 독립변수에 따라 차이가 있었으며, 그 차이는 모두 통계적으로 유의한 차이를 보였다(p<0.05). 상관관계 분석 결과, 조사야 크기 및 AJD, BD, PD는 성별, 연령과는 상관성이 없었으며, 신장과는 약한 양의 상관관계를 보였고, 체중과 체질량지수와는 중간 정도 양의 상관관계를 나타냈다. 본 연구의 결과 요추 측면 검사 시 CS는 신장과 체중, BMI와 상관성이 있는 것으로 나타났다. 그리고 임상에서 조사야 크기 조절시 체중과 체질량지수를 고려하여 중심 엑스선의 입사점을 척추 돌기관절로 이동시킨다면, 약 5%의 조사야 크기 감소 효과를 기대할 수 있을 것으로 생각된다.

• 한국미생물·생명공학회지
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• 제26권2호
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• pp.122-129
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• 1998

최근 생물방제균으로 주목받고 있는 Enterobacter cloacae의 방제기작이 이 균에 의해 토양내에서 생산된 휘발성 ammonia이며 ammonia의 생산에는urease가 관계한다는 보고를 근거로 하여, 항생물질 생산성 균주로 선발된 우수한 길항균주에 암모니아 생성능, 즉 urease 유전자를 유전적으로 부가함으로써 항진균성 길항물질 생산과 암모니아 생산이 동시에 이루어 질 수 있는 새로운 다기능의 생물방제균을 유전적으로 육종하고자 하였다. 저병해 인삼경작지로부터 식물근부균 Fusarium solani의 생육을 강하게 억제하는 길항세균 한 균주 SH14균주를 분리, 선발하였으며, 분리된 균주를 동정한 결과 Bacillus subtilis이거나 그 근연종으로 추정되었다. 억제기작 실험을 통해 길항균주 B. subtilis SH14에 의해 생산되는 항진균성 길항물질은 외막가수분해효소와 같은 고분자 물질이 아니라 열에 안정한 저분자의 항생물질임을 알 수 있었다. 한편 ammonia 생산을 위한 urease의 유전자는 urease 생산력이 강력한 호알칼리성 Bacillus pasteurii의 urease 생산유전자를 E. coli-Bacillus shuttle vector인 pEB203에 subcloning하였고, 이어서 pGU 366으로 명명된 이 recombinant plasmid를 선발된 항진균성 길항균주 B. subtilis SH14에 PEG-induced protoplast transformation 방법으로 도입, 발현시켰으며, 최적조건을 조사하여 90분간의 lysozyme 처리과정 후 1.5 $\mu\textrm{g}$/$m\ell$의 DNA와 40% PEG4000의 첨가로 약 6.5$\times$$10^{-4}$의 형질전환율을 얻을 수 있었다. 아울러 암모니아 생성능이 부가된 생물방제균 B. subtilis SH14(pGU366)에 의해 식물근부균 F. solani에 대한 생육억제력이 증가되는지 여부를 억제거리 측정법과 균체중량법을 통해 확인한 결과 urease 유전자가 도입된 형질전환체 B. subtilis SH14(pGU366)의 근부균 생육억제능이 각각 36.7%, 44.0%정도로 숙주균주인 B. subtilis SH14에 비해 근부균 생육억제능을 보다 강하게 나타내었음을 알 수 있었다. 따라서 항진균성 항생물질 생산성 생물방제균 B. subtilis SH14에 외부의 urease유전자를 도입하여 ammonia 생성능을 부가함으로써 생물방제력의 상승효과를 거둘 수 있었다.