• 한국발생생물학회지:발생과생식
• /
• 제15권3호
• /
• pp.223-230
• /
• 2011

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants derived from incomplete combustion of carbons and crude oil. In this study, we investigated the effects of benzo[a]pyrene (B[a]P), a representative PAHs on in vitro sex steroid hormone production and germinal vesicle breakdown (GVBD) using isolated oocytes of longchin goby (Chasmichthys dolichognathus) and chameleon goby (Tridentiger trigonocephalus). Oocytes in diameters of 0.8-0.9 (end vitellogenic stage) and 0.9-1.0 mm (germinal vesicle migratory stage) from longchin goby and 0.5 mm (fully vitellogenic stage) from chameleon goby were used. In GVBD assay, B[a]P at 10 nM stimulated GVBD in the oocytes of 0.8-0.9 mm from longchin goby. B[a]P at 1 nM stimulated GVBD in the oocytes with diameter 0.5 mm from chameleon goby. In steroid production from oocytes of longchin goby, B[a]P at 100 nM decreased testosterone (T) production, B[a]P at 1,000 nM increased estraiol-17 (J (E2) production and 10 and 100 nM increased $17,20{\beta}$-dihydroxy-4-pregnen-3-one ($17{\alpha}20{\beta}P$) production in the oocytes with diameter 0.8-0.9 mm. B[a]P at 1,000 nM increased E2 production, 100 and 1,000 nM increased $17{\alpha}20{\beta}P$ production in the oocytes with diameter 0.9-1.0 mm. In steroid production of oocytes from chameleon goby, B[a]P at 1,000 nM increased $E_2$ production. B[a]P at 10 nM increased $17{\alpha}20{\beta}P$ production. In the ratio of $E_2$ to T ($E_2$/T), B[a]P at 100 and 1,000 nM increased $E_2$/T in the oocytes of longchin goby. B[a]P at 100 nM also increased $E_2$/T in the oocytes of chameleon goby. Taken together, these results suggest that B[a]P have not only weak estrogenic effects but progestogenic effects on oocyte maturation.

• 한국잠사곤충학회지
• /
• 제39권2호
• /
• pp.180-185
• /
• 1997

To develope the novel baculovirus transfer vector, the p10 gene was cloned from the Bombyx mori nuclear polygedrosis virus (BmNPV) vB2 strain isolated from the B. mori larvae of sericultural farms. The novel transfer vector was constructed by using the p10 gene of BmNPV vB2 strain was 210 bp. The TAAG sequence at the -71 bp of upstream from translation initiator ATG and two polyadenylation signal site at the downstream from terminator TAA were also detected in the p10 gene. The 5' and 3' flanking region of the p10 gene amplified by PCR was cloned into pBluescriptII SK(+) and then transfer vector pBm10 was construceted. The 7.9 kb pBm10 was analysed by restriction enzymes and the map was confirmed. In order to determine the expression of foreign gene of pBm10, $\beta$-galactosidase gene was inserted in the SmaI site of foreign gene cloning site of pBm10. The pBm10 containing $\beta$-galactosidase gene was cotranfected wth genomic DNA of BmNPV vB2 into BmN-4 cells. The recombinant baculovirus expressing $\beta$-galactosidase was also produced polygedra in the infected cells. The results indicated that pBm10 is functional, suggesting that in the baculovirus expression vector system, the recombinant virus produced by pBm10 was effective by oral infection for the producing recombinant proteins in in vivo expression.

• The Korean Journal of Ecology
• /
• 제21권2호
• /
• pp.169-174
• /
• 1998

This is the review of vegetational history of the post-glacial period in Korea. most of studies for vegetational changes are located in the lowland alluvial plain, especially below the hilly zone of western and eastern coastal regions of Korea. A couple of methods, pollen analysis and radiocarbon dating, have been employed in these studies. These results lead us to establish the pollen zonation in Korea as the followings. Yasuda et al.(1980) classified six period in Yongrang lake of sokcho. These are as follow I. 17,000-15,000yr B.P.: Picea, Abies, Pinus(Haploxylon), Larix stage, II.15,000-10,000yr B.P:Herb, Pteridophyta stage, III.10.000-6,700yr BP.:Quercus stage, IV.6,700-4,500yr B.P.:Pinus, Quercus, Carpinus stage, V.4,500-1,400yr B.P.:Quercus. Pinus stage, VI.1,400yr B.P.-present: Pinus, Herbs stage. Jo(1979) also divided the period into two stages from the outcomes of analysis done in Jumoonjin and other sites I.10,000-6,000yr B.P.:Quercus stage, II.6,000-present: Pinus-Quercus stage, and three substages: IIa.6,000-3,400 yr B.P.:lower Pinus stage, IIb.3,400-2,000yr B.P.:Pinus-Quercus stage, IIc.2,000-present: Pinus stage. Choi(1993, 1996) divided the period into three stages: I.6,000-5,000yr B.P.:Alnus, Quercus stage, II.5,000-4,000yr B.P.: Alnus, Quercus, Pinus stage, III.4,500-2,600yr B.P.: Alnus, Pinus stage. In the period around 6,000yr B.P. distinct dominant species clearly occupied the lowland of the eastern and western coasts. Thus, this strongly supports the fact that even if Korea experienced its warm and wet climate after the lateglacial, it underwent a different environmental change, dry climate, compared to the regions of Japan.

• 한국축산식품학회지
• /
• 제40권2호
• /
• pp.183-196
• /
• 2020

The protective effect of pig skin gelatin water extracts (PSW) and the low molecular weight hydrolysates of PSW generated via enzymatic hydrolysis with Flavourzyme® 1000L (LPSW) against scopolamine-induced impairment of cognitive function in mice was determined. Seventy male ICR mice weighing 20-25 g were randomly assigned to seven groups: Control (CON); scopolamine (SCO, 1 mg/kg B.W., intraperitoneally (i.p.); tetrahydroaminoacridine 10 [THA 10, tacrine; 10 mg/kg B.W. per oral (p.o.) with SCO (i.p.)]; PSW 10 (10 mg/kg B.W. (p.o.) with SCO (i.p.); PSW 40 (40 mg/kg B.W. (p.o.) with SCO (i.p.); LPSW 100 (100 mg/kg B.W. (p.o.) with SCO (i.p.); LPSW 400 (400 mg/kg B.W. (p.o.) with SCO (i.p.). All treatment groups, except CON, received scopolamine on the day of the experiment. The oxygen radical absorbance capacity of LPSW 400 at 1 mg/mL was 154.14 μM Trolox equivalent. Administration of PSW and LPSW for 15 weeks did not significantly affect on physical performance of mice. LPSW 400 significantly increased spontaneous alternation, reaching the level observed for THA and CON. The latency time of animals receiving LPSW 400 was higher than that of mice treated with SCO alone in the passive avoidance test, whereas it was shorter in the water maze test. LPSW 400 increased acetylcholine (ACh) content and decreased ACh esterase activity (p<0.05). LPSW 100 and LPSW 400 reduced monoamine oxidase-B activity. These results indicated that LPSW at 400 mg/kg B.W. is a potentially strong antioxidant and contains novel components for the functional food industry.

• Journal of Nutrition and Health
• /
• 제29권8호
• /
• pp.881-888
• /
• 1996

The purpose of this study was to determine whether vitamin B6(B6) deficiency affects fuel utilization and blood cholesterol profile with exercise-training. Twenty-four rats were fed a B6 deficient(-B6) diet or a control (+B6) diet for 5 weeks and either exercised(EX) or nonexercised (NE). EX rats were exercised on treadmill(10$^{\circ}$, 0.5-0.8km/h) for 20 minutes everyday. Glucose(GLU), glycogen (GLY), protein(PRO), trglyceride(TG), free fatty acid(FFA), total cholesterl(TC), HDL-cholesterol(HDL-C) and LDL-choleterol(LDL-C) were compared in plasma(P), liver(L) and skeletal muscle(M) of rats. There was a vitamin effect on the level of P-GLU, P-TG, M-TG, L-GLY, L-PRO and an exercise effect on the level of P-PRO, P-FFA, M-PRO, L-GLY, L-TG, P-TC, P-HDL-C, P-LDL-C. Compared to +B6 rats were lower and there were no differences in P-GLU, P-FFA, P-TG. M-GLY, L-TG, P-TC and P-HDL-C. In EX group, the level of P-TG was higher and M-PRO was lower in -B6 rats. There were no differences in M-GLY, L-TG, P-TC and P-HDL-C. These results suggest that a lowered intake of vitamin B6 may impair the adaptation of animals to fuel metabolism related to a decrease of fatty acid oxidation and attenuates the exercise-traning effect on blood lipid profile.

• 충청수학회지
• /
• 제28권3호
• /
• pp.473-482
• /
• 2015

Let Qf be the maximal derivative of f with respect to the Bergman metric $b_B$. In this paper, we will find conditions such that $(1-{\parallel}z{\parallel})^s(Qf)^p(z)$ is bounded on B. We will also find conditions such that Bergman projection type operator $P_r$ is bounded operator from $L^p(B,d{\mu}_r)$ to the holomorphic Besov p-space Bs $B^s_p(B)$ with weight s.

• 대한수학회논문집
• /
• 제10권2호
• /
• pp.305-315
• /
• 1995

The class $R_{\gamma-1,p}(A,B,\alpha)$ for $-1 \leq B < A \leq 1,\gamma > (B -1)p+(A_B)(p-\alpha)/1-B$ and $0 \leq \alpha < p$ consisting of p-valently analytic functions in the open unit disc is defined with the help of convolution technique. We study containment property, integral transforms and a sufficient condition for an analytic function to be in $R_{\gamma-1,p}(A,B,\alpha)$.

• 대한수학회보
• /
• 제55권4호
• /
• pp.1209-1219
• /
• 2018

We provide a direct proof of the following theorem of Kalton, Hollenbeck, and Verbitsky [7]: let H be the Hilbert transform and let a, b be real constants. Then for 1 < p < ${\infty}$ the norm of the operator aI + bH from $L^p(\mathbb{R})$ to $L^p(\mathbb{R})$ is equal to $$\({\max_{x{\in}{\mathbb{R}}}}{\frac{{\mid}ax-b+(bx+a){\tan}{\frac{\pi}{2p}}{\mid}^p+{\mid}ax-b-(bx+a){\tan}{\frac{\pi}{2p}}{\mid}^p}{{\mid}x+{\tan}{\frac{\pi}{2p}}{\mid}^p+{\mid}x-{\tan}{\frac{\pi}{2p}}{\mid}^p}}\)^{\frac{1}{p}}$$. Our proof avoids passing through the analogous result for the conjugate function on the circle, as in [7], and is given directly on the line. We also provide new approximate extremals for aI + bH in the case p > 2.

• 대한수학회보
• /
• 제60권4호
• /
• pp.863-872
• /
• 2023

Let n ⩾ 2 be an integer, we denote the smallest integer b such that gcd {(ⁿ_k) : b < k < n - b} > 1 as b(n). For any prime p, we denote the highest exponent α such that p^α | n as v_p(n). In this paper, we partially answer a question asked by Hong in 2016. For a composite number n and a prime number p with p | n, let n = a_mp^m + r, 0 ⩽ r < p^m, 0 < a_m < p. Then we have $$v_p\(\text{gcd}\{\(n\\k\)\;:\;b(n)1\}\)=\{\array{1,&&a_m=1\text{ and }r=b(n),\\0,&&\text{otherwise.}}$$

• Toxicological Research
• /
• 제12권1호
• /
• pp.17-20
• /
• 1996

The effect of Fumonisin B1, a mycotoxin produced by Fusarium moniliforme on bacterial viruses P1 and Lambda, was investigated by the virus plaque assay. Fumonisin B1 inhibited the P1 viral multiplication in the concentration range from $100{\mu}g$/ml to $400{\mu}g$/ml. The inhibition was Fumonisin B1 concentration-dependent. Another bacterial virus Lambda multiplication was also inhibited by lower concentration of Fumonisin B1 ($10{\mu}g$/ml~$50{\mu}g$/ml). This inhibition was dependent on Fumonisin B1 and on virus-Fumonisin B1 reaction time. Sensitivity of bacteriophage Lambda to Fumonisin B1 was higher than that of P1 virus. Lambda vital DNA was treated in vitro with Fumonisin B1 at various concentration. Significant DNA fragmentation by Fumonisin 191 was observed in the agarose gel electrophoresis. Lambda viral DNA was partially digested even in the Fumonisin B1 $10{\mu}g$ and the level of its fragmentation was dependent on Fumonisin B1 amount up to $30{\mu}g$ per assay.