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Hemagglutination of Hantaan and Seoul Viruses (한탄바이러스와 서울바이러스의 혈구응집반응)

  • Seong, In-Wha;Song, Ki-Joon;Lee, Ho-Wang
    • The Journal of the Korean Society for Microbiology
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    • v.21 no.2
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    • pp.227-231
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    • 1986
  • The hemagglutination activities of Hantaan virus and Seoul virus were demonstrated. The hemagglutinins were prepared by sucrose-acetone extraction method from suckling mouse brains infectecd with Hantaan and Seoul viruses. Hemagglutination of goose erythrocytes by these viral hemagglutinins was pH dependent in phosphate buffer system. Hantaan and Seoul viruses were distinguished by pH range of hemagglutination. 76/118 and 79/90 strains of Hantaan virus showed hemagglutination at the range of pH 5.75-6.4 and the optimal pH was 5.75 with the titer of 1:512 in 76/118 and 1:256 in 79/90. In contrast, KSNUSD 84/34 strain of Seoul virus revealed hemagglutination at the range of pH 6.2-6.4 and the optimal pH was 6.4 with the titer of 1 : 64.

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Influence of UV Irradiation Interval on Mineral Content in Metatarsus of Broiler Chicks (자외선 조사간격이 브로일러 병아리의 중족골 광물질 함량에 미치는 영향)

  • 장윤환;조인호;여영수;이은택;배은경;김중달
    • Korean Journal of Poultry Science
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    • v.20 no.3
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    • pp.133-140
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    • 1993
  • A study was conducted to investigate the concentrations of Ca, P and ash in metatarsal bone of broiler chicks exposed to UV light in different Interval. Day-old Hubbard broiler chicks (199=10 control+3 irradiation interval $\times$ 9 elapsed time $\times$ 7 replicate) were fed vitamin D3 deficient diet for 3 wk in a windowless subdued-light room and exposed to 297 nm UVB light by 0.068 mJ/$\textrm{cm}^2$ three times In 0, 12 or 24 h interval. The metatarsal bones were taken at 0, 6, 12, 18, 24, 48, 96, 144 or 240 h after last irradiation, separated from adhering tissue, ether extracted, dried and ashed. The Ca concentration was measured by atomic absorption spectrophotometry and P by ammonium metavanadate colorimetry. When the birds were continuously exposed to UVB light for 30 min without interval, the Ca content in metatarsus increased gradually according to the time after irradiation and reached the highest value 16.75% at 240 h after exposure. The P content also increased gradually until 144 h, where it was 9.75%. The ash content in metatarsus increased continuously until 240 h, the final time in this research, where 42.75% was shown. As 10 min three times irradiation in 12 h interval was applied to the chicks, the metatarsal Ca presented a small peak(13.31%) at 12 h after irradiation and a large peak(16.91%) at 144 h. P content showed a small peak(7.18%) at 12 h and a large level(8.34%) at 240 h. Ash content increased continuously until 240 h, where it was 46.53%. The small peaks in Ca and P concentration were thought to be derived from preirradiation at 12 and 24 h before final irradiation for 10 min. When 24 h interval system was treated, the peak value of Ca content(24.18%) occurred earlier(96 h) than those in 0 and 12 h interval systems. P content also showed the maximum value at 96 h(7.29%). Ash content presented an increasing trend until 240 h, where 45.75% was appeared. In respecting the method of UVB irradiation, the peak value of Ca content in metatarsus appeared earlier in 24 h interval system than in other systems. Meanwhile the ash contents in metatarsus of birds exposed to UVB light in 12 and 24 h interval procedures were higher than those in 0 h interval one. Therefore, it was concluded that a daily 10 min irradiation of UVB light would be desirable for increasing the Ca and ash content in metatarsus of brolier chicks.

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Stability of Anti-Yeast Activities and Inhibitory Effects of Defatted Green Tea Seed Extracts on Yeast Film Formation (탈지 녹차씨 추출물의 항효모 활성 안정성 및 산막 형성 억제능 평가)

  • Yang, Eun Ju;Seo, Ye-Seul
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.3
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    • pp.327-334
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    • 2017
  • Water and 75% ethanol extracts were prepared from defatted green tea seeds and evaluated for their anti-yeast activities. The antimicrobial activities of defatted green tea seed extracts (DGTSEs) were tested against food-spoilage bacteria, yeasts, and molds. DGTSEs exhibited antimicrobial activities with minimum inhibitory concentrations of $39{\sim}1,250{\mu}g/mL$ against three bacteria, two molds, and all tested yeast strains. Ethanol extract showed higher antimicrobial activity than water extract. The stability of anti-yeast activities of DGTSEs was examined under different conditions of temperature, pH, and NaCl concentrations. The anti-yeast activities of DGTSEs were stable at pH 3~9, 0~20% NaCl, and $100^{\circ}C$ for 30 min. However, anti-yeast activities of DGTSEs decreased upon heating at $70^{\circ}C$ for 24 h or $121^{\circ}C$ for 15 min. DGTSEs were applied to food models to determine their inhibitory effects on yeast film formation. Water and 75% ethanol extracts were effective in preventing yeast film formation at concentrations more than 156 and $39{\mu}g/mL$ in soy sauce, 156 and $78{\mu}g/mL$ in pickle sauce, and 78 and $39{\mu}g/mL$ in kimchi, respectively.

Feeding Black Bengal Goat under Intensive Management : Milk Feeding in Pre-weaned Kids

  • Chowdhury, S.A.;Faruque, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.1
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    • pp.39-45
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    • 2004
  • Despite higher fecundity, Black Bengal goat generally has lower milk production, which is often insufficient for their multiple kids. However, milk requirement of Black Bengal kids is poorly defined. Present trial was thus designed to determine the requirement and intake of milk of pre-weaned Black Bengal kids in relation to their growth rate age and sex. Thirty, one month old Black Bengal kids of either sex divided into two groups were allocated to two groups, suckling alone (T1) or suckling along with teat-bottle feeding of milk (T2). Ten kids were allocated for the T1 and 20 kids were allocated for the T2. Digestibility of milk was also determined in T2 animals. Average daily consumption of milk, milk energy and milk N were 773 and 722 g, 1,170 and 1,093 kJ/kg $W^{0.75}/d$ and 1,552 and 1,462 mg N/kg $W^{0.75}/d$, respectively. During 9 weeks trial period, male kids had significantly higher live weight (4.32 vs. 4.20 kg; p<0.01) and intake of milk (773 vs. 722 g/d; p<0.05), energy (117 vs. 1,093 kJ/kg $W^{0.75}$/d; p<0.01) and N (1,552 vs.1,462 mg/k $W^{0.75}/d$; p<0.01) than the female. Milk consumption and the corresponding milk energy and milk N intake increased linearly up to 5th week of the trial, which, then decreased quadratically up to 9th week i.e., up to the weaning. Overall milk DM intake during this period was 2.58% (range 1.5-3.0%) of live weight or about 36 g/kg $W^{0.75}/d$ (range 29-45 g/kg $W^{0.75}/d$). Pre-weaned Black Bengal kids of about 4.5 kg weight, growing at the rate of 60 g weight daily requires at least 750 g milk daily. Daily live weight gain, estimated as the regression between the live weights over time, was 60 g ($r^2$=0.99) and 55 g ($r^2$=0.99), respectively, for the male and female kids. Efficiency of milk energy utilization for weight gain ranged from 0.67 to 0.84 (mean 0.81) for the male kid and 0.75 to 0.91 (mean 0.82) for the female kids. Efficiency of milk protein utilization for weight gain ranged from 0.46 to 0.70 (mean 0.61) for the male kid and 0.51 to 0.81 (mean 0.64) for the female kids. Additional teat-bottle feeding of suckling kids had no significant effect on their growth rate (54vs. 57 g/d). Average digestibility of milk DM, OM and N was 98.85, 98.99 and 98.69%, respectively and they were slightly (p>0.05) higher in the male than the female kids. Results suggest that the requirement of energy and protein and their utilization efficiency in Black Bengal kids is not different from that of the other breeds of goat.

Use of Duckweed as a Protein Supplement for Breeding Ducks

  • Men, Bui Xuan;Ogle, Brian;Lindberg, Jan Erik
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.6
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    • pp.866-871
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    • 2002
  • An experiment was conducted at the experimental duck farm of Cantho University to determine the effects of feeding duckweed (Lemna minor) that replaced commercial protein supplements in diets for local and Cherry Valley breeding ducks. The experiment included a total of 180 ducks, with five treatments and three replicates and six breeding ducks (one male plus five females together) per replicate, for both local and exotic Cherry Valley ducks. The five diets were fed ad libitum and were based on rice byproducts supplemented with roasted soya bean meal plus dried fishmeal at levels of 100% (SF100, control), 75 (SF75), 50 (SF50), 25 (SF25) or zero (SF0) % of the protein in the control diet, corresponding to 18, 15, 13, 10 and 8% CP in the diets for both breeds, respectively. Fresh duckweed was supplied ad libitum for all treatments. These diets were fed to local breeding ducks from 7 to 12 months of age, and to exotic breeding ducks (Cherry Valley) from 8 to 13 months of age. Total mean daily dry matter (DM) intakes were 183, 178, 176, 177 and 174 g (p<0.05) for the local ducks, and 221, 208, 215, 219 and 210 g (p<0.01) for the exotic ducks for the SF100 (control), SF75, SF50, SF25 and SF0 diets, respectively. Laying rates of the local ducks were 66.5, 65.2, 62.9, 63.1 and 62.3%, and of the Cherry Valley ducks 61.9, 58.4, 58.9, 59.1 and 53.5% (p<0.001) for the control (SF100), SF75, SF50, SF25 and SF0 treatments, respectively. Fertile egg rates were 95.6, 95.6, 97.8, 97.8 and 92.2%, and hatchabilities 89.4, 80.6, 87.2, 88.6 and 77.8% (p<0.05) for the local breed, and 97.8, 97.8, 91.1, 92.2 and 90.0% (p<0.05) and 72.8, 74.7, 75.0, 74.3 and 76.7% for the Cherry Valley ducks for diets SF100, SF75, SF50, SF25 and SF0, respectively. Corresponding feed conversion ratios (dry matter basis) were 3.83, 3.82, 3.89, 4.01 and 3.96 kg feed per kg egg mass for the local ducks and 4.52, 4.56, 4.58, 4.73 and 5.02 kg feed per kg egg mass for the Cherry Valley ducks for the SF100, SF75, SF50, SF25 and SF0 treatments, respectively. Replacement of 100% of the protein supplement by fresh duckweed in the diets of the local laying ducks decreased the feed costs by 25% compared to the control diet.

Characteristics of Rice Mutants Resistant to 5- Methyltryptophan (벼 5-methyltryptophan 저항성 돌연변이체의 특성)

  • 이효연;강권규;노일섭;이춘환;권혜경;박현숙
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.5
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    • pp.637-643
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    • 1995
  • TR75, a rice (Oryza sativa L. var. Sasanishikj) mutant resistant to 5-methyltryptophan (5MT) was segregated from the progenies of its initial mutant line, TR1. The 5MT resistance of TR75 was inherited in the M$_{8}$ generations as a single dominant nuclear gene, and was also expressed in callus derived from seeds, roots, and anthers as well as in the seedlings. The callus induced from these organs could grow at 50 mg/1 of 5MT, whereas the growth of wild-type callus was completely inhibited even at 25 mg/1. The seedlings of TR75 did not show resistance to L-azetidine-2-carboxylic acid, S-2-aminoethyl-L-cysteine, p-fluoro-DL-phenylalanine. The content of free amino acids in the TR75 homozygous seeds increased approximately 1.5 to 2.0 fold compared to wild-type seeds. Especially, the contents of tryptophan, phenylalanine and aspartic acid were 5.0, 5.3 and 2.7 times higher than those of wild-type seeds, respectively.y.

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Role of Methacholine PC20 in FEF25-75% for the Diagnosis of Bronchial Asthma

  • Son, Kyeong Min;Jang, Seung Hun;Kang, Hye Ryun;Han, Bo Ram;Kim, Joo Hee;Kim, Hyun Sung;Park, Sung Hoon;Hwang, Yong Il;Kim, Dong Gyu;Jung, Ki Suck
    • Tuberculosis and Respiratory Diseases
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    • v.67 no.4
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    • pp.311-317
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    • 2009
  • Background: The methacholine bronchial provocation test is a useful tool for evaluating asthma in patients with normal or near normal baseline lung function. However, the sensitivity of this test is 82~92% at most. The purpose of this study is to evaluate the clinical usefulness of $FEF_{25-75%}$ in identification of airway hyperresponsiveness in patients with suspected asthmatic symptoms. Methods: One hundred twenty-five patients who experienced cough and wheezing within one week prior to their visiting the clinic were enrolled. Results: Sixty-four subjects showed no significant reduction of $FEV_{1}$ or $FEF_{25-75%}$ on the methacholine bronchial provocation test (Group I). In 24 patients, $FEF_{25-75%}$ fell more than 20% from baseline without a 20% fall of $FEV_{1}$ during methacholine challenge (Group II). All patients who had more than 20% fall of $FEV_{1}$ (n=37) also showed more than 20% of reduction in $FEF_{25-75%}$ (Group III). Baseline $FEV_{1}$/FVC (%) and $FEF_{25-75%}$ (L) were higher in group II than group III (81.51${\pm}$1.56% vs. 75.02${\pm}$1.60%, p<0.001, 3.25${\pm}$0.21 L vs. 2.45${\pm}$0.21 L, p=0.013, respectively). Group II had greater reductions of both $FEV_{1}$ and $FEF_{25-75%}$ than group I at 25 mg/mL of methacholine (p<0.001). The provocative concentration of methacholine causing a 20% fall in $FEF_{25-75%}$ in group II was about three-fold higher than that in group III. Conclusion: A 20% fall of $FEF_{25-75%}$ by methacholine provocation can be more sensitive indicator for detecting a milder form of airway hyperresponsiveness than $FEV_{1}$ criteria.

Pressure on Sacrum and Buttock according to Tilt Table Inclination (기립경사대 각도 증가에 따른 천골과 둔부 압력 변화)

  • Yuk, Goon-Chang
    • The Journal of Korean Physical Therapy
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    • v.25 no.2
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    • pp.71-75
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    • 2013
  • Purpose: Although use of a tilt table is recommended in clinical practice, there are no published guidelines regarding pressure and inclination for tilt table use. The aim of the current study was to assess the changes of pressure on sacrum and buttock according to different inclination of the tilt table in healthy subjects. Methods: Thirty two healthy subjects participated in this study. Subjects were positioned supine on the tilt table and safety straps were secured across the chest, pelvic, and knee with sufficient tension to prevent the subjects from falling. Pressure and peak pressure of sacrum and buttock were measured using pressure mapping system with the tilt table standing at $0^{\circ}$, $15^{\circ}$, $30^{\circ}$, $45^{\circ}$, $60^{\circ}$, $75^{\circ}$, and $85^{\circ}$ inclination. Results: A significant decrease in the pressure of sacrum and buttock was achieved by increasing tilt table inclination (p<0.05): $0^{\circ}{\sim}15^{\circ}$ (8.16%), $15^{\circ}{\sim}30^{\circ}$ (8.02%), $30^{\circ}{\sim}45^{\circ}$ (11.61%), $45^{\circ}{\sim}60^{\circ}$ (16.18%), $60^{\circ}{\sim}75^{\circ}$ (16%), and $75^{\circ}{\sim}85^{\circ}$ (11.48%). A significant decrease in the peak pressure was achieved by increasing tilt table inclination (p<0.05): $30^{\circ}{\sim}45^{\circ}$ (9.91%), $45^{\circ}{\sim}60^{\circ}$ (19.24%), $60^{\circ}{\sim}75^{\circ}$ (19.93%), and $75^{\circ}{\sim}85^{\circ}$ (11.48%). No significant peak pressure change was observed in $0^{\circ}{\sim}15^{\circ}$, $15^{\circ}{\sim}30^{\circ}$ tilt table inclination (p>0.05). Conclusion: The results of this study showed that the pressure of sacrum and buttock were decreased according to increasing tilt table inclination in healthy subjects. Guidelines are needed in order to optimize patient safety and overall outcome for tilt table standing.

Evaluation of the Potential of Retrograde Flow Competition in the Right Gastroepiploic Artery Graft for Coronary Artery Bypass Grafting (우위대망동맥을 이용한 관상동맥우회술 후 역행성 혈류 발생가능성의 연구)

  • Chung, Bong-Kyu;Sun, Kyung;Kwon, Joon;Kim, Kwang-Ho;Jung, Jae-Seung;Son, Ho-Sung;Lee, Sung-Ho;Kim, Kwang-Taik;Kim, Hyung-Mook
    • Journal of Chest Surgery
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    • v.35 no.1
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    • pp.20-26
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    • 2002
  • Background: Due to the concern of flow competition or retrograde steal, it has been generally suggested that the right gastroepiploic artery(RGEA) pedicled graft should be used in critical coronary stenosis lesion. The study was designed to evaluate the potential of retrograde flow competition in the RGEA pedicled graft by measuring the native pressure differences(PD) between the normal coronary artery and celiac arterial pressure, which would be compared with trans-stenosis pressure gradients(TSPG) in coronary artery occlusive disease. Material and Method: Between July, 1998 and February, 1999, pressures of the right coronary artery and the right gastroepiploic artery(or the celiac artery) were measured in patients with the patent right coronary artery(n=12). The PD between the arteries was compared with the TSPG in the occlusive coronary arteries(n=32). Result: The pressures of the normal right coronary artery and celiac artery were 143$\pm$23 vs. 134$\pm$17mmHg in systole(p<0.005), 74$\pm$13 vs. 73$\pm$14mmHg in diastole(p=NS), and 100$\pm$16 vs. 97$\pm$15mmHg in mean (p<0.05). The PD between the arteries were -8~25mmHg in systole, -4~7mmHg in diastole, and -1~10mmHg in mean. The TSPG measured in the occlusive coronary arteries were -4~19(7$\pm$5.8)mmHg in the lesion less than 75% stenosis vs. 7~74(27$\pm$18.3)mmHg in the 75% or over stenosis lesion(p<0.005). The normally existing pressure difference between the coronary arteries and RGEA(15~20mmHg) was significantlyless than the TSPG in .the occlusive coronary artery with 75% or over stenosis(p<0.001). Conclusion: If the pressure gradient between the RGEA and the coronary artery distal to the stenosis is the main determinant of development of retrograde flow competiton in the RGEA pedicled graft, the above data suggests that there will be little chance of competition when It is used in the coronary lesion with 75% or over stenosis.

A study on the estimation of TMDL run-off pathway coefficients for livestock resources (축분자원화물의 총량관리지침 배출구조계수 산정에 관한 연구)

  • Han, Gee-Bong;Lee, Young-Sin
    • Journal of the Korea Organic Resources Recycling Association
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    • v.21 no.3
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    • pp.99-107
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    • 2013
  • In this study, field scale test was conducted to estimate the TMDL coefficients (runoff and leachate:(${\beta}4$)(${\beta}5$)(${\beta}8$)) for the livestock resources applying to agricultural crop land as fertilizer, and the results were obtained as follows. Each waste reduction coefficient(${\beta}4$) was shown to be in the range of 0.94~0.75 for public waste treatment plants and 0.99~0.83 for private waste treatment in the analysis of BOD, COD, SS, T-N, T-P, TOC. Thus private plants showed higher rate. Waste treatment discharge into the land coefficient (${\beta}5$) was also shown to be in the range of 0.4.~0.24 for public plants and 0.75~0.16 for private plants, so it is much lower than other coefficients. However SS and T-P were shown to be much higher for land discharge in private plants than in public plants. Treatment coefficient in the public plants (${\beta}8$) appeared to be average 0.75 for T-P but over 90% treatment efficiency and also large deviation were observed due to 0.2 of some other treatment plants.