• 제목/요약/키워드: p75

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Pseudomonas oleovorans의 유가식 배양에 의한 medium chain length Polyhydroxyalkanoates (MCL-PHA) 생산

  • 김범수;임희연
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2000년도 춘계학술발표대회
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    • pp.207-210
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    • 2000
  • P. oleovorans의 유가식 배양에서 탄소원으로 octanoic acid, 질소원으로 $NH_4NO_3$를 이용한 혼합기질을 배양액의 pH 변화에 따라 공급하는 pH-stat 기질공급전략을 개발하였다. 공급기질의 탄소원/질소원 비 (C/N 비)를 변화시킴으로써 최종 균체농도, PHA 농도, PHA 함량 등을 변화시킬 수 있었으며, 최대 균체농도는 C/N 비가 10 (g octanoic acid/g $NH_4NO_3$)일 때 65 g/L, 최대 PHA 농도는 C/N 비가 20일 때 41 g/L, 최대 PHA 함량은 C/N 비가 20일 때 75%였으며 최대 PHA 생산성은 C/N 비가 10일 때 1.03 g/L/h였다.

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중금속 오염토의 Electrokinetic 정화 처리시 pH 발현과 납 제거의 전극 간 특성 (The Characteristics of pH Variations and Lead transport during Electrokinetic Remediation of soil Contaminated by Heavy Metal)

  • 한상재;김수삼
    • 한국지하수토양환경학회지:지하수토양환경
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    • 제6권4호
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    • pp.13-23
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    • 2001
  • 본 연구는 중금속 오염지반의 정화를 목적으로, Electrokinetic(EK) 기법을 적용 할 때 지반 내에서 pH와 오염물 분포특성을 알아보았다. 시료 내에서의 EK 처리로 인한 납 제거 효과를 살펴본 결과 1V/cm의 전압경사 하에서 납은 음극으로 이동하여, 전극 간 시료의 80%정도 영역에서 제거율은 대략 75% 정도에 이르렀다. 그러나 이동된 납은 대부분이 음극영역에 침전되어 궁극적으로 음극수로 배출되어 제거되는 납의 양은 미미하였다. 이때, EK 정화처리는 시료의 대부분인 80% 정도에서 토양환경보전법 상의 규준치를 만족할 수 있으나, 음극쪽 20%지역은 별도의 향상기법을 적용하여 처리하는 기법이 필요하다.

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대체 감미료와 low methoxyl pectin을 이용한 저열량 pectin gel의 제조 (Replacement of sucrose with other sweeteners and low methoxyl pectin in low caloric pectin gels)

  • 오혜숙;이명희;문수재
    • 한국식품조리과학회지
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    • 제9권4호
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    • pp.289-292
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    • 1993
  • 당알코올의 대체 감미료로서의 유용성을 검토하기 위한 목적으로 LMP jelly들을 제조하여 품질 특성을 비교하였다. 관능검사 결과 LMP jelly의 감미도는 설탕의 50%를 maltitol로 대치하였을때 현저하게 감소하였고, 반면 같은 50%를 3종류의 당알코올로 혼합 사용한 경우에는 제조시 당알코올의 대체 가능성을 높음을 시사하였다.

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소석회와 CO2를 이용한 상수관로의 부식제어(I) - 소석회 주입공정의 최적 운전인자 도출 (Corrosion Control in Water Distribution System using Lime and Carbon Dioxide(I) - Determination of Optimum Operational Conditions in Lime Adding Process)

  • 손병용;변규식;김영일;이두진
    • 상하수도학회지
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    • 제22권3호
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    • pp.373-378
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    • 2008
  • The pH & alkalinity adjustment method by lime and carbon dioxide($CO_2$) for corrosion control in water distribution system was investigated to determine the optimum operational condition in lime adding process in water treatment plant(WTP). The mixing time at dissolution tank and sedimentation time at saturator for maintaining optimal turbidity condition of lime supernatant were 60~75 minutes and 75~95 minutes, respectively. There was no difference according to $CO_2$ adding methods such as $CO_2$ saturated water or $CO_2$ gas. But, $CO_2$ saturated water could be convenience at WTP in terms of pH control and quantitative dosing. To minimize generation of calcium carbonate products, the short time interval between adding of lime and $CO_2$ is most important. The lime should be added below 32 mg/l for preventing pH rising and generation of calcium carbonate products at the heating condition.

호알카리성 Bacillus sp.의 cyclodextrin glycosyltransferase의 정제와 특성 (Purification and Characterization of Cyclodextrin Glycosyltransferase from Alkalophilic Bacillus sp)

  • 정용준;공인수;유주현;강윤숙
    • 한국미생물·생명공학회지
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    • 제18권1호
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    • pp.44-48
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    • 1990
  • 토양에서 분리한 호알카리성 Bacillus sp. YC-335가 생산하는 CGTase를 ethanol 침전법, DEAE-Toyopearl column chromatography, Sephadex G-100 column chromatography 방법 등에 의해 대량 정제 하였다. 이때 수율은 17.8 이었고 52.9배의 정제도를 가진 효소단백질을 얻을 수 있었다. 정제효소는 SDS-polyacrylamide gel 전지영동에 의해 분자량이 75,000 정도인 단일 peptide의 단백질임을 확인하였고 효소의 최적활성 pH는 6.0 있었으며 pH 안정성은 pH 6-10까지 안정하였다. 최적활성온도는 5$0^{\circ}C$이었으며 열안정성은 5$0^{\circ}C$까지 안정하였고 이는 15mM CaCl$_2$에 의해 열안정성이 보호되었다.

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EFFECT OF SUPPLEMENTING UREA MOLASSES MINERAL BLOCK LICK TO STRAW BASED DIET ON DM INTAKE AND NUTRIENT UTILIZATION

  • Garg, M.R.;Gupta, B.N.
    • Asian-Australasian Journal of Animal Sciences
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    • 제5권1호
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    • pp.39-44
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    • 1992
  • Twelve male crossbred calves of 18 months of age were divided into two groups of six each. Animals in both the groups were fed wheat straw ad lib. However, animals in group II had free access to urea molasses mineral block (UMMB) lick. Straw DM intake (kg/100 kg B. Wt. and $g/w^{0.75}\;kg$) was significantly (p < 0.01) higher in group II ($1.95{\pm}0.06$; $75.55{\pm}1.79$) as compared to group I ($1.27{\pm}0.08$; $48.77{\pm}2.43$). Straw DM digestibility coefficient was not significantly different in groups I and II. However, DOMI (kg/100 kg B. Wt.) was significantly (p < 0.01) higher in group II ($0.986{\pm}0.05$) as compared to group I ($0.615{\pm}0.03$). Digestibility coefficient of DM, OM and CP were significantly higher in group II as compared to group I. However, digestibility coefficients of EE, CF and NFE were non-significantly different between the two groups. Animals in group II exhibited significantly (p < 0.01) higher and positive N, Ca and P balances as compared to group I which exhibited negative balances. Total-N, ammonia-N and urea-N in the blood plasma of animals in group II were significantly (p < 0.01) higher as compared to group I.

Effects of Intra-abdominal Pressure with Visual Feedback on Muscle Activation of Upper Trapezius and Sternomastoid during Forced Inspiration in Individuals with Costal Respiration

  • Kim, Kwang-Su;Shin, Hwa-Kyung
    • The Journal of Korean Physical Therapy
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    • 제32권2호
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    • pp.75-79
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    • 2020
  • Purpose: This study analyzed the immediate effects of intra-abdominal pressure with visual feedback on the muscle activation of the upper trapezius and sternomastoid during natural inspiration and forced inspiration in individuals with costal respiration. Methods: The eighteen individuals with upper costal breathing pattern participated in this study. Surface electromyography was used to analyze the muscle activity of the upper trapezius and sternomastoid during natural inspiration and forced inspiration before and after intra-abdominal pressure. Results: A significant difference in muscle activation was observed with the muscle type, inspiration type, and test session (p<0.05). The muscle activities of the sternomastoid and upper trapezius decreased significantly during forced inspiration after intra-abdominal pressure training (p<0.05). On the other hand, there was no significant difference during natural inspiration in both muscles (p>0.05). A comparison of the difference between the pre-test and post-test during forced inspiration revealed the upper trapezius to be significantly larger than the sternomastoid (p<0.05). No significant difference was noted during natural inspiration (p>0.05). Conclusion: The intra-abdominal pressure has positive effects on correcting the breathing patterns in individuals with costal respiration.

DFB 구조형 플라즈마 다중모드 간섭 결합기를 사용한 편향기의 구현 (Implementation of Polarization Beam-Splitter based on DFB-Assisted Plasmonic Multimode Interference Coupler)

  • 호광춘
    • 한국인터넷방송통신학회논문지
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    • 제13권5호
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    • pp.143-148
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    • 2013
  • 2개의 플라즈마 다중모드 간섭 결합기 (P-MMIC)와 DFB 전송구조를 결합하여 하나의 새로운 초소형 광 편향기를 구현하였다. $2{\times}1$$1{\times}2$ P-MMIC를 연결하는 내부 DFB 구조로부터 반사되거나 투과되는 TE, TM 모드들의 편향된 전력들을 P-MMIC를 통하여 출력되도록 설계하였다. 수치해석 결과 설계된 DFB 구조형 플라즈마 다중모드 간섭 결합기의 크기는 약 $75{\mu}m$로 매우 작았으며, 낮은 삽입손실과 높은 분리비율을 보였다. 더욱이, DFB 전송구조의 대역폭은 약 20 nm로 광대역 특성을 나타내었다.

세포외 Cytosine Deaminase의 효소학적 성질 (Enzymatic Properties of Extracellular Cytosine Deaminase)

  • 유대식;김대현;박정문;송형익;정기택
    • 미생물학회지
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    • 제26권4호
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    • pp.368-374
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    • 1988
  • Bacillus polymyxa YL38-3이 생성하는 세포의 cytosine deaminase의 효소학적 ,성질을 검토하였다. 본 세포외 효소는 열 안정성이 높으며, 인산완충액(pH6.0)과 $30^{\circ}C$에서 효소활성이 최대를 나타냈다. 본 효소는 cytosine 뿐 아니라 5- fluorocytosine-을 기질로 하나, 5-methylcytosine은 촉매하지 않았다. 더우기 본 효소는 $Cd^{2-}$, $Hg^{2+}$의 중금속이온과 ImM p-chloromercuribenzoate에 의하여 완전히 실활되며, o-phenanthroline과 monoiodoacetate에 의하여 75% 저해되었다. 그러나 1mM 2-mercaptoethanol에 의하여 본 효소의 활성을 약 200% 이상 활성화시켰다.

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Characteristics of low-nitrite pork emulsified-sausages with paprika oleoresin solution during refrigerated storage

  • Kim, Geon Ho;Chin, Koo Bok
    • Journal of Animal Science and Technology
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    • 제63권2호
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    • pp.394-404
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    • 2021
  • The objective of this study was to evaluate quality characteristics of low-nitrite emulsified-sausages (ESs, < 75 ppm) containing paprika oleoresin solution (POS) for replacing sodium nitrite (NaNO2). Pork ESs were prepared with four treatments (reference (REF), 150 ppm NaNO2; TRT1, 0 ppm NaNO2 + 0.1% POS; TRT2, 37.5 ppm NaNO2 + 0.1% POS; and TRT3, 75 ppm NaNO2 + 0.1% POS). The physicochemical and texture properties, microbial counts, residual nitrite and thiobarbituric acid reactant substances (TBARS) were measured during refrigerated storage of 35 days. Although TRT2 and TRT3 had lower levels of NaNO2, they had higher redness and yellowness than REF (p < 0.05). Microbial counts of total bacterial counts and Enterobacteriaceae of TRT2 and TRT3 were similar to those of REF (p > 0.05). Expressible moisture percentages (EM, %) of TRT2 and TRT3 were lower than those of REF (p < 0.05). TBARS values of TRT2 and TRT3 were not different from those of REF (p > 0.05). Among treatments, TRT1 had the highest TBARS values (p < 0.05). In conclusion, 0.1% POS in combination with 37.5 ppm NaNO2 would have quality characteristics similar to those of REF. Therefore, approximately 3/4 of the initial nitrite level could be replaced with 0.1% POS, and eventually developed healthier pork products.