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Effect of Nano Particles on the Hathcing rate of Artemia sp. Cyst Zooplankton (나노입자가 알테미아(Artemia sp.) Cyst 부화율에 미치는 영향)

  • Jeong, Yeon-Kyu;Lee, Byeong-Woo;Park, Chan-Il;Choi, Kwang-Soo;Kim, Mu-Chan
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.12 no.4
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    • pp.302-306
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    • 2009
  • 9 kinds of nanoparticle used for this study was a particle with the size of less than 100 nm of diameter, and Artemia sp. cyst examined what kind of influence to have upon the process hatched out in nauplius. 82% hatched in nauplius at the opposition ward where a nanoparticle wasn't added after 24 time course. AGZ020, Nano silver, P-25, Sb and SnO nanoparticle showed hatching rate of 18%, 20%, 13%, 50% and 0% respectively by the 20mg/L density, and it became clear that a harmful effect is big, but I had a harmful effect compared with the opposition ward by 75%, 60%, 73% and 73% respectively by Ag-$TiO_2$, In, Sn and Zn nanoparticle, but a feeble thing was known relatively compared with AGZ020, Nano silver, P-25, Sb and SnO nanoparticle. The difference has caused this with the ingredient a nanoparticle has. Ag is included 2 % and AGZ020, Nano silver and P-25 nanoparticle are used widely as anti-fungus agent, and the SnO nanoparticle which became combination is a light catalyst pill, and oxygen is used for a Sn particle. This and others, a possibility that use is generalized and flows into aquatic environment in sequence the home electronics, functionality cosmetics, anti-fungus agent and a light catalyst pill at present becomes high for nanoparticles and others. The anxiety which has an influence on the ecology world in the water with this can be generated, so I'd have to study the potential danger a nanoparticle has continuously.

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Feeding rate, excreting rate and biomass increasing rate of earthworm (Eisenia fetida) fed with paper mill sludge and cow dung manure (제지슬러지 및 우분 급이시 줄지렁이(Eisenia fetida)의 섭식률, 배설률 및 증체율)

  • Bae, Yoon-Hwan;Park, Kwang-Il
    • Journal of the Korea Organic Resources Recycling Association
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    • v.14 no.3
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    • pp.101-109
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    • 2006
  • Earthworms(Eisenia fetida) were fed with paper mill sludge produced from P&G or Yuhan Kimberly paper manufacturing factory, or cow dung manure. Turnover rate of feed into earthworm biomass and excreting rate on each feed were investigated on dry weight base. Biomass of earthworm population was increased on paper mill sludge, but it was fallen to death on cow dung manure, which had high electrical conductivity and low redox potential. When P&G paper mill sludge was supplied to adult, elder juvenile or younger juvenile for 84 days, turnover rate of feed into earthworm biomass was 0.48, 0.40 and 0.76%, respectively, and on Yuhan Kimberly paper mill sludge 0.26, 0.45, 0.42%. When P&G paper mill sludge was supplied to adult, elder juvenile or younger juvenile, excreting rate was 49.02, 54.32 and 55.39%, respectively and on Yuhan Kimberly paper mill sludge 32.22, 41.86, 40.69%, and on cow dung manure, 73.73, 57.89. 76.38%.

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Biomass Production of One-year-old Hybrid Poplars and Its Estimation (식재 당년생 신품종 포플러류의 Biomass 생산량 및 생산량 추정)

  • Koo, Yeong-Bon;Shim, Sang-Yung;Noh, Eui-Rae
    • Journal of Korea Foresty Energy
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    • v.7 no.1
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    • pp.22-27
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    • 1987
  • Above ground biomass production of one-year-old hybrid poplars was investigated to select the best clones which are suitable for short rotation forestry. The results were as follows: 1. Average dry weight of Populus alba X P. glandulosa Fl clones was 2.86 for stem,0.41 for branches 0.70 for leaves and 3.97 t/ha for total. and the ratios of green weight to dry weight were 0.47 for stem,0.36 for branches,0.31 for leaves and 0.42 for total. Dry weight of Populus nigra X P. maximowiczii Fl clones was 1.48 for stem, 0.54 for branches.0.71 for loaves and 2.73t/ha for total, and the ratios of green weight to dry weight were 0.42 for stem. 0.37 for branches, 0.28 for leaves and 0.36 for total. 2. Biomass production (green and dry weight)of Populus alba X P. glandulosa F1 clones can be predicted by diameter measurement only, but biomass production of Populus nigra X P. maximowiczii F1 clones can not be predicted by any of investigated characteristics. 3. Dry weight distribution of pepulus alba X P. glandulosa Fl clones were 73$\%$ for stem, 10$\%$ for branches and 17$\%$ for leaves, and dry weight distribution of P. Populus nigra X P. maximowiczii F1 clones were 54$\%$ for stem, 20$\%$ for branches and 26$\%$ for leaves.

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Effect of Nano Particles on the Hathcing rate of Artemia sp. Cyst (알테미아(Artemia sp.) Cyst 부화율에 미치는 나노입자의 영향)

  • Lee, Byeong-Woo;Cho, Sang-Man;Park, Chan-Il;Jeong, Woo-Gun;Kim, Mu-Chan
    • Proceedings of KOSOMES biannual meeting
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    • 2008.05a
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    • pp.137-141
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    • 2008
  • 9 kinds of nanoparticle used for this study was a particle with the size of less than 100 nm of diameter, and Artemia sp. cyst examined what kind a influence to have upon the process hatched out in nauplius. 82% hatched in nauplius at the opposition ward where a nanoparticle wasn't added after 24 time course. AGZ020, Nano silver, P-25, Sb and SnO nanoparticle showed hatching rate of 18%, 20%, 13%, 50% and 0% respectively by the 20mg/L density, and it became clear that a harmful effect is big, but I had a harmful effect compared with the opposition ward by 75%, 60%, 73% and 73% respectively by Ag-$TiO_2$, In, Sn and Zn nanoparticle, but a feeble thing was known relatively compared with AGZ020, Nano silver, P-25, Sb and SnO nanoparticle. The difference was mused this with the ingredient a nanoparticle has. Ag is included 2% and AGZ020, Nano silver and P-25 nanoparticle are used widely as anti-fungus agent, and the SnO nanoparticle which became combination is a light catalyst pill, and oxygen is used for a Sn particle. This and others, a possibility that use is generalized and flows into aquatic environment in sequence the home electronics, functionality cosmetics, anti-fungus agent and a light catalyst pill at present becomes high for nanoparticles and others. The anxiety which has an influence on the ecology world in the water with this can be generated, so I'd have to study the potential danger a nanoparticle has continuously.

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Comparison of the Result of Radiation Alone and Chemoradiation in Cervical Cancer (자궁 경부암에서 방사선 단독치료와 방사선 및 화학요법 병행치료의 비교)

  • Kim, Jae-Cheol;Park, In-Kyu
    • Radiation Oncology Journal
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    • v.13 no.2
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    • pp.191-198
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    • 1995
  • Purpose: This analysis was to compare the result of radiation alone and chemoradiation in cervical cancer in terms of response, survival, failure, and complication. Materials and Methods: A retrospective analysis of 135 cervical cancer patients treated with definitive radiotherapy from November 1985 to December 1991 was performed. Fifty-six patients were treated with radiation alone and 79 patients were treated with cisplatin-based chemotherapy plus radiation. Follow-up period ranged from 5 to 105 months with a median 47 months. According to the FIGO classification, the patients were subdivided into 18 $(13.3\%)$ stage IB, 7 $(5.2\%)$ stage IIA, 97 $(71.9\%)$ stage IIB, and 9 $(6.7\%)$ stage IIIB. Results: A complete response was noted in 51 patients $(91.1\%)$ of the radiation alone group, and 68 patients $(86.1\%)$ of the chemoradiation group. There was no statistical difference in complete response rate between the two groups. Overall survival rate at 5 years was $73.3\%$. According to stage, overall survival rates at 5 years were $88.9\%$ in stage IB, $85.7\%$ in stage IIA, $73.8\%$ in stage IIB, and $37.5\%$ in stage IIIB, respectively. According to treatment modality, overall survival rates at 5 years were $81.9\%$ in the radiation alone group, $67.0\%$ in the chemoradiation group (p=0.22). Disease-free survival rate at 5 years were $70.4\%$ in the radiation alone group. $68.5\%$ in the chemoradiation group (p=0.85) Locoregional control rates at 5 years were $76.1\%$ in the radiation alone group, $73.8\%$ In the chemoradiation group (p=0.70). Distant disease-free survival rates at 5 years were $83.9\%$ in the radiation alone group, $90.3\%$ in the chemoradiation group (p=0.59). Treatment-related bone marrow suppressions were noted in 3 $(5.4\%)$ patients of the radiation alone group, 14 patients $(17.7\%)$ of the chemoradiation group (p(0.05). Grade 2 vesical complications were noted in 14 patients of the radiation alone group. and 10 Patients of the chemoradiation group. Grade 2 rectal complications were noted in 2 patients of the radiation alone group, and 3 Patients of the chemoradiation group. One case of rectal perforation was noted in the chemoradiation group, and grade 2 small bowel obstructions were noted in 2 patients of the radiation alone group. There were no statistical differences in the incidence of vesicar, rectal, and small bowel complicaions between the two groups. Conclusion: No statistical difference was found between the radiation alone group and the chemoradiation group in terms of response, survival, and failure. but the incidence of bone marrow suppression was higher in the chemoradiation group.

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Usage of Library Collection, Services & Assistance Among Ophthalmologists - An Opinion Survey

  • Govindarajan, R.;Dhanavandan, S.
    • International Journal of Knowledge Content Development & Technology
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    • v.10 no.2
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    • pp.39-49
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    • 2020
  • Accurate and reliable Information improves decision making, reduces costs, and saves time. The ophthalmic Libraries supply quality ophthalmic information to the ophthalmologists to serve the patients in a better way and stimulate the growth of ophthalmic field. This paper aims to study the usage of library collection, services and assistance among the Ophthalmologists - Eye Doctors in India. A survey method is used to obtain the ophthalmologists' usage of library collection, services and assistance. Through convenient sampling method, a structured questionnaire is circulated to the ophthalmologists in India and 633 ophthalmologists are responded. Among the 633 ophthalmologist, 82.15% of the ophthalmologists use the library collection - book. 73.46% of the ophthalmologists use the online data / journals. 89.73% of the ophthalmologists seek assistance to locate books/articles/documents. There exists a significant difference between the ophthalmologists use of Library Service and gender (p value 0.001**). There exists a significant difference between the ophthalmologists use of Library Assistance / help and designation category (p value 0.000**). There exists a significant difference between the ophthalmologists use of Library Assistance / help and working experience (p value 0.017**). There exists a significant difference between Library Services (p value 0.009**), Library Assistance / help (p value 0.000**) and institution type. The study results will help the ophthalmic libraries to serve the user better.

Antimicrobial Activity of Lactobacillus plantarum Lp2 Isolated from Kimchi (김치에서 분리한 Lactobacillus plantarum Lp2의 항균작용)

  • 박연희;송현주
    • Microbiology and Biotechnology Letters
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    • v.19 no.6
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    • pp.637-643
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    • 1991
  • The inhibitory activity of Lactobacillus plantarum Lp2 isolated from Kimchi was investigated against E. colz, Staphylococcus aureus, Str. faecalis, P. aeruginosa and psycrotrophic strain Pcl. The addition of ether extract from Lp2 culture broth completely inhibited the growth of E, coli, P. aeruginosa and Pc1, but the inhibitory action was weak against S. aureus and Str. faecalis. The inhibitory substance(s), soluble in methanol, acetone and ethyl ether, was active in the pH range of 5 and 6, but the activity was lost above pH 6. The activity was retained after heating at $121^{\circ}C$ for 15 min. By dialysis, the molecular weight of the inhibitory substancek) was estimated under 1000. The TLC analysis of Lp2 ether extract revealed the presence of three bands and the inhibitory activity was found in that of $R_f$ value 0.73.

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The Applicability of SWAT-APEX Model for Agricultural Nonpoint Source Pollution Assessment (농업 비점오염원 평가를 위한 SWAT-APEX 모델의 적용성 검토)

  • Jung, Chung-Gil;Park, Jong-Yoon;Lee, Ji-Wan;Jung, Hyuk;Kim, Seong-Joon
    • Journal of The Korean Society of Agricultural Engineers
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    • v.53 no.5
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    • pp.35-42
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    • 2011
  • This study is to check the applicability of SWAT-APEX (Soil and Water Assessment Tool-Agricultural Policy / Environmental eXtender) model as combined watershed and field models by applying the APEX to paddies in a watershed (465.1 $km^2$) including Yedang reservoir. Firstly, the SWAT were calibrated with 3 years (2000~2002) daily streamflow and monthly water quality (T-N and T-P) data, and validated for another 3 years (2003~2005) data. The average Nash-Sutcliffe model efficiency (ME) of streamflow during validation was 0.73, and the coefficient of determination ($R^2$) of T-N and T-P were 0.77 and 0.73 respectively. Next, running the SWAT-APEX model with the SWAT calibrated parameters for paddies, the $R^2$ of T-N and T-P were 0.80 and 0.76 respectively. The results showed that SWAT-APEX model was more correctly predicted for T-N and T-P loads than SWAT model. The difference results between watershed and field models was predicted to have substantial impact on NPS loads, especially on T-N and T-P loads. Therefore, to improve negative NPS load simulations should be considered the model characteristics as simulating mechanism to properly select the NPS model for agricultural watershed.

Cloning of a Gene Encoding Dextranase from Lipomyces starkeyi and its Expression in Pichia pastoris

  • Kang, Hee-Kyoung;Park, Ji-Young;Ahn, Joon-Seob;Kim, Seung-Heuk;Kim, Do-Man
    • Journal of Microbiology and Biotechnology
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    • v.19 no.2
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    • pp.172-177
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    • 2009
  • A gene(lsd1) encoding dextranase from Lipomyces starkeyi KSM22 has been previously cloned, sequenced, and expressed in Saccharomyces cerevisiae. The gene consisting of 1,824 base pairs and encoding a protein of 608 amino acids was then cloned into and secretively expressed in Pichia pastoris under the control of the AOX1 promoter. The dextranase productivity of the P. pastoris transformant(pPIC9K-LSD1, 134,000 U/I) was approximately 4.2-fold higher than that of the S. cerevisiae transformant(pYLSD1, 32,000 U/I) cultured in an 8-1 fermentor. Over 0.63 g/l of active dextranase was secreted into the medium after methanol induction. The dextranase of the P. pastoris transformant, as analyzed by SDS-PAGE and Western blotting, showed only one homogeneous band. This dextranase of the P. pastoris transformant showed a broad band near 73 kDa. Rabbit monoclonal antibodies against a synthetic LSD1 peptide mix also recognized approximately 73 kDa.

Expression of Fusion Products of Insecticidal Crystal Protein Genes from Two Different Bacillus thuringiensis Strains (두종의 Bacillus thuringiensis 내독소단백질 유전자의 융합에 의한 발현)

  • 제연호;김상현
    • Journal of Sericultural and Entomological Science
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    • v.35 no.1
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    • pp.36-42
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    • 1993
  • Expression of insecticidal protein by fusion product of truncated HD-1[CryIA(a)] N-terminal and HD-73[CryIA(c)] C-Terminal fragment of Bacillus thruingiensis subsp. kurstaki was investigate. Immunological analysis of transformants by using polyclonal antisera raised against the whole-crystal protein of HD-1 revealed that SK4 and SK5 were observed cross-reaction with polypeptides of 77-kDa and 105-kDa, respectively. Bioassay of the transformant pSK5 to Plutella maculipennis and Heliothis assulta were 96% and 97%, respectively.

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