• Journal of Embryo Transfer
• /
• v.20 no.2
• /
• pp.147-156
• /
• 2005

본 연구는 NCSU-23과 PZM-3 배양액에 EGF, $\beta-ME$와 glucose의 첨가가 돼지 난자의 체외성숙에 미치는 영향과 배양조건을 다르게 하여 계대배양한 섬유아세포를 이용한 핵이식배를 다른 배양액과 산소조건에서 배양하였을 때 체외발생율에 미치는 영향을 조사하였다. 핵이식 배를 20ng/ml EGF를 첨가 또는 첨가하지 않은 NCSU-23 및 PZM-3 배양액에서 배양하였을 때 배반포로의 체외 발생율은 각각 $12.0\pm1.3\%,\;9.6\pm1.9\%,\;10.9\pm2.1\%,\;9.1\pm2.3\%$였다. 핵이식 배를 $25{\mu}M\;\beta-ME$를 첨가 또는 첨가하지 않은 NCSU-23 및 PZM-3 배양액에서 144시간 배양하였을 때 배반포로의 체외 발생율은 각각 $9.6\pm1.7\%,\;7.3\pm2.3\%,\;11.9\pm1.8\%$와 $7.4\pm2.1\%$였다. $\beta-ME$를 첨가한 PZM-3 배양액에서 배양하였을 때 배반포로의 체외 발생율은 $\beta-ME$를 첨가하지 않은 배양액에서 배양한 배보다 높은 체외발생율을 나타냈다. (p<0.05). 핵이식 배를 1.5mM glucose를 첨가 또는 첨가하지 않은 NCSU-23 및 PZM-3 배양액에서 배양하였을 때 배반포로의 체외 발생율은 각각 $9.4\pm2.2\%,\;6.8\pm2.7\%,\;10.9\pm2.4\%$와$8.9\pm2.6\%$였다. Glucose를 첨가한 NCSU-23과 PZM-3 배양액에서 배양하였을 때 배반포로의 체외 발생율은 glucose를 첨가하지 않은 배양액에서 배양한 배보다 높은 체외 발생율을 나타냈다. 핵이식 배를 NCSU-23 및 PZM-3 배양액과 $5\%$ 및 $20\%$ 산소 조건에서 배양하였을 때 배반포로의 체외 발생율은 각각 $11.1\pm1.8\%,\;9.8\pm1.4\%,\;12.5\pm1.6\%$와$10.9\pml.5\%$였다. NUSU-23과 PZM-3 배양액에서 $5\%$ 산소 조건에서 배양하였을 때 $20\%$산소 조건에서 배양한 난자보다 높은 체외 발생율을 나타났다. 섬유아세포를 NCSU-23 배양액에서 배양하여 공여자세포로 이용하여 10 및 $11\~15\;passage$ 이내로 계대배양하였을 때의 융합율은 $60.0\~73.3\%,\;52.5\%$였다. 섬유아세포를 PZM-3 배양액에서 배양하여 공여자세포로 이용하여 10 및 $11\~15passage$ 이내의 계대배양시의 융합율은 $60.4\~75.1\%$ 및 $58.7\%$였다.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.43 no.4
• /
• pp.291-300
• /
• 2007

The crayfish-shaped artificial bait for octopus drift line is manufactured, into which is made to insert ordinary bait. The effects of the artificial bait are confirmed through the analysis of octopus' behavior in the laboratory in comparison with the existing bait of pig-fat skin, and the investigation of the octopus catch on the sea, and in addition, the examination of the water quality in relation to the bait to be inserted into the artificial bait is done together. The artificial bait is red in color, 10.5cm in the length of the body, and 29g in weight. The octopus behavior in relation to the bait in the laboratory has shown a good result in which more than 30% of the octopuses rushed to the artificial bait. In the analysis of the octopus sitting time by the bait, the octopus stayed longer with the artificial bait(63.7%) than the pig-fat skin(25.1%). The octopus sitting time at the artificial bait inserted with frozen squid(48.8％, 44.6％) is shown to be longer than that of the pig-fat skin(36.9％) or boiled fish paste(21.2％). In the analysis of sitting frequency to the bait, the case of the artificial bait(total 17 times) was more than that of the pig-fat skin(total 3 times), and the case of the artificial bait with frozen squid attracted the octopuses more frequently than that of the pig-fat skin or the boiled fish paste. In the field experiment, the fishing boat A(Manseon-ho, 1.22tons) caught the total 93 octopuses, while the fishing boat B(Ilho-ho, 0.73tons) caught the total 154 octopuses, all of which weighed less than 9.0kg. In the comparison with the total catch, the case with the artificial bait was a little higher than that of the pig-fat skin($^{**}p\;<\;0.05$). In the analysis of the water quality in relation to the baits, the COD showed the descending order of frozen squid(0.57mg/g), boiled fish paste(0.18mg/g), and pig-fat skin(0.10mg/g), and the case with frozen squid was the highest and the case of the pig-fat skin, lowest. The total phosphorus, like the COD, showed the highest in frozen squid(0.02mg/g), and in case of the total nitrogen, unlike the COD, the pig-fat skin was shown to be the highest(0.006mg/g).

• The Korean Journal of Food And Nutrition
• /
• v.28 no.6
• /
• pp.1065-1070
• /
• 2015

A high-yield Korean rice cultivar cv. Boramchan and Hanmaeum, and rice cultivar for use in staple cv. Hopum were prepared and investigated for their physiological characteristics. Water content, water holding capacity, amylose content, damaged starch content, particle size, and pasting properties of the rice flours were measured. Moisture content of the Boramchan and Hanmaeum, made under wet and dry milling conditions, were as follows: wet conditions (14.79% and 13.56% respectively) and dry conditions (7.98% and 7.14% respectively). Water holding capacity of the Boramchan and Hanmaeum made by wet milling condition was 236.67% and 231.35%, respectively. Water holding capacity of the samples made by dry milling condition showed a higher score compared with other samples. The amylose content of Hopum, Boramchan, and Hanmaeum made by dry milling condition were 19.12%, 19.55% and 19.59%, respectively. Damaged starch contents of the samples made by wet milling showed a lower score. Final viscosity of Hopum, Boramchan, and Hanmaeum made by wet milling condition was 2,604, 3,052, and 2,917 cp, respectively. In this study, the results indicated that the super-yield Korean rice flour made by wet milling condition tends to show a lower water holding capacity, damaged starch contents and particle size, as compared to controls. However, a setback of the super-yield Korean rice flour was a higher score as compared to the controls, regardless of the milling conditions.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
• /
• 2005.10a
• /
• pp.111-114
• /
• 2005

1. 증체량 이유자돈 시부터 비육돈 완성기간까지 비육 전체 기간동안의 증체량은 다음과 같이 분석되었다. 처리별 암컷의 증체량은 생봉독처리 2구에서 99.08kg으로 가장 높았고, 생봉독처리 1구에서 99.08kg, 무처리구에서 95.88kg, 생봉독처리 3구에서 93.50kg 순으로 최고치와는 5.58kg의 차이를 보여주었으며 대조구와는 3.2kg 증체량이 높아 3.3% 증체율이 높았다. 거세돈에서의 처리별 증체중은 생봉독 처리 2구에서 102.23kg으로 가장 높았고, 다음은 생봉독처리 1구에서 101.79kg, 무처리구에서는 88.96kg, 생봉독 3처리구에서는 95.76kg의 순으로 나타냈다. 따라서 최고치와 최저치의 차이는 6.47kg으로 유의한 수준차(p<0.05)로 나타났으며 대조구보다는 3.2kg의 증체량 차이로서 암컷과 같은 수준으로 나타났다. 2. 사료효율 비육 전체기간 동안의 처리별 암수간 평균 두당 사료섭취량과 사료효율은 다음과 같다. 암컷의 평균 사료효율은 생봉독 처리 1구에서 2.55로 처리구중에서 가장 우수하였고, 다음은 생봉독처리 2구와 대조구에서 2.56으로 동일하게 분석되었으며, 생봉독 3처리구에서 가장 저조한 2.62의 순으로 분석되어 사료효율 0.07 차이로 2.3%의 차이를 보여주었다. 발육성적이 가장 높은 생봉독 2처리구에서는 255kg의 사료를 섭취하여 2.54의 최적의 사료효율을 보여주어 사료 효율면에서도 생봉독 2처리구에서 가장 경제성이 높은 것으로 분석되었다. 3. 육성율 및 생산지수 폐사수는 생봉독 처리 3구에서 2두(자웅 각 1두)가 발생하여 육성율 95%로 처리구중에서 가장 저조하였고, 다음은 대조구 1두가 발생하여 육성율 97.5%를 보여주었는데 비하여 생봉독처리 1구와 2구에서는 폐사돈이 발생하지 않아 육성율 100%를 보여주었다. 생산지수는 대조구를 100으로 기준하였을 때 생봉독처리 2구에서 107.87로 가장 우수하였으며, 생봉독 처리 1구에서 106.22, 가장 저조한 처리구는 폐사수가 많은 생봉독 처리 3구에서 94.73으로 나타내었다. 이상과 같이 생봉독 처리 2구에서 가장 우수한 생산효과를 나타내었다. 4. 생산성 향상 효과 처리별 대조구(100) 대비 사료섭취량은 생봉독 처리 3구에서 97.22로 가장 낮았고, 기타 처리구는 1.19${\sim}$1.59의 처리를 보이고 있는데 비하여, 대조구(100) 대비 증체율에서는 생봉독 처리 2구에서 103.30으로 3.30%가 높았다.

• Journal of Life Science
• /
• v.20 no.1
• /
• pp.40-48
• /
• 2010

This study was carried out to investigate the physicochemical and functional properties of Yacon (Polymnia Sonchifolia) powder. The proximate composition of Yacon powder as a dry matter basis was 3.53% moisture content, 1.13% crude protein, 0.40% crude fat, 0.79% crude ash, 1.63% dietary fiber and 92.52% carbohydrate. The major free sugars were identified as fructose and glucose. Analysing total amino acids, 18 kinds of components were isolated from Yacon powder. The essential amino acid contained in Yacon powder accounted for 28.40% of total amino acid, while the non-essential amino acid accounted for 73.61%. Analysing total fatty acids, only 2 kinds - palmitic acid and lauric acid - were detected. Oxalic acid was the major organic acid. The contents of vitamin A, vitamin C and vitamin E were 0.057 mg%, 0.670 mg% and 0.001 mg%, respectively. The mineral contents of Yacon powder were in the order of Zn

• Korean Journal of Food Science and Technology
• /
• v.35 no.1
• /
• pp.1-6
• /
• 2003

Chemical compositions of several herbs (rosemary, mint, thyme, sage, and lavender) cultivated in Korea were analyzed. Approximate compositions were as follows: moisture $69.92{\sim}82.10%$, crude ash $2.48{\sim}6.15%$, crude fat $0.40{\sim}2.46%$, crude protein $0.84{\sim}1.57%$, and crude fiber $2.48{\sim}6.15%$. Total contents of phenolics determined by Folin-Dennis's method were in the range of $73.24{\sim}197.79mg%$. Contents of minerals, Na, Ca, Mn, P, Mg, Zn, and Fe determined by ICP-AES were $43.0{\sim}112.5,\;177.5{\sim}304.0,\;0.5{\sim}1.5,\;74.0{\sim}218.5,\;57.0{\sim}116.0,\;1.0{\sim}2.0$ and $3.0{\sim}5.0mg%$, respectively. Free sugar contents determined by HPLC were: sucrose $0{\sim}7.61$, glucose $0.94{\sim}15.92$, and rhamnose $0.64{\sim}7.99mg%$. Fatty acids including palmitic, stearic, oleic, linoleic, linolenic, and arachidonic acids were identified by GC. Linoleic and linolenic acid contents were higher than those of palmitic and stearic acids. Aroma components identified by GC-MS were 1,8-cineole, ${\alpha}-phellandrene,\;{\alpha}-terpinene,\;{\beta}-pinene,\;{\beta}-thujone$, borneol, butan-1-ol, cis-sabinene hydrate, ${\delta}-carene,\;{\gamma}-terpinene$, and verbenone.

• Fisheries and Aquatic Sciences
• /
• v.21 no.2
• /
• pp.6.1-6.10
• /
• 2018

The intention of this study was to identify the bacterial pathogens infecting Oreochromis niloticus (Nile tilapia) and Clarias gariepinus (African catfish), and to establish the antibiotic susceptibility of fish bacteria in Uganda. A total of 288 fish samples from 40 fish farms (ponds, cages, and tanks) and 8 wild water sites were aseptically collected and bacteria isolated from the head kidney, liver, brain and spleen. The isolates were identified by their morphological characteristics, conventional biochemical tests and Analytical Profile Index test kits. Antibiotic susceptibility of selected bacteria was determined by the Kirby-Bauer disc diffusion method. The following well-known fish pathogens were identified at a farm prevalence of; Aeromonas hydrophila (43.8%), Aeromonas sobria (20.8%), Edwardsiella tarda (8.3%), Flavobacterium spp. (4.2%) and Streptococcus spp. (6.3%). Other bacteria with varying significance as fish pathogens were also identified including Plesiomonas shigelloides (25.0%), Chryseobacterium indoligenes (12.5%), Pseudomonas fluorescens (10.4%), Pseudomonas aeruginosa (4.2%), Pseudomonas stutzeri (2.1%), Vibrio cholerae (10.4%), Proteus spp. (6.3%), Citrobacter spp. (4.2%), Klebsiella spp. (4.2%) Serratia marcescens (4.2%), Burkholderia cepacia (2.1%), Comamonas testosteroni (8.3%) and Ralstonia picketti (2.1%). Aeromonas spp., Edwardsiella tarda and Streptococcus spp. were commonly isolated from diseased fish. Aeromonas spp. (n = 82) and Plesiomonas shigelloides (n = 73) were evaluated for antibiotic susceptibility. All isolates tested were susceptible to at-least ten (10) of the fourteen antibiotics evaluated. High levels of resistance were however expressed by all isolates to penicillin, oxacillin and ampicillin. This observed resistance is most probably intrinsic to those bacteria, suggesting minimal levels of acquired antibiotic resistance in fish bacteria from the study area. To our knowledge, this is the first study to establish the occurrence of several bacteria species infecting fish; and to determine antibiotic susceptibility of fish bacteria in Uganda. The current study provides baseline information for future reference and fish disease management in the country.

• Journal of Korean Medicine Rehabilitation
• /
• v.23 no.1
• /
• pp.77-86
• /
• 2013

This study was introduced the short-term effectiveness of Oriental medicine treatment for lumbar disc herniation inpatients. We selected 208 inpatients with a magnetic resonance imaging(MRI)-confirmed lumbar disc herniation since June 2012 until August 2012. Their demographics characterisitics, numeric rating scale(NRS) and Oswestry back-related disability index(ODI) for low or leg pain, lumbar flexion and extension angle were assessed at baseline and when discharged from the hospital. When it measured the straight leg raisingscale(SLR), the low side of the angle was examined. Treatment every patient has received as follow; herbal medicine, bee venom, acupuncture and physical examination, spinal manipulation. NRS of low back pain and leg pain and ODI is $5.6{\pm}2.3$, $4.9{\pm}2.8$ and $45.5{\pm}20.0$, respectively at baseline and $2.8{\pm}1.8$, $2.7{\pm}1.9$ and $27.2{\pm}14.1$, respectively when discharged from the hospital. range of motion(ROM) of lumbar flexion and extension angle and SLR is $70.0{\pm}27.1$, $15.4{\pm}7.2$ and $61.3{\pm}23.0$ respectively at baseline and $80.5{\pm}16.9$, $18.25{\pm}4.1$ and $73.2{\pm}14.0$, respectively when discharged from the hospital. It shows that statistically significant improved(P<0.001). Furthermore, regarding patient satisfaction with the treatment, excellent(33.7%), good(55.4%), normal(10.4%), poor(0.5%), it suggests that most patients(89%) satisfied with the treatment.

• Journal of Oral Medicine and Pain
• /
• v.26 no.1
• /
• pp.59-73
• /
• 2001

This study was performed to investigate the effects of occlusal appliance on the mandibular position and the mandibular rotational torque movement during speech. For this study, 20 patients with temporomandibular disorders(TMDs) and 20 normal subjects without any signs and symptoms in the masticatory system were selected as the patient group and as the normal group, respectively. Biopak $system^{(R)}$(Bioresearch Inc., Milwaukee, USA) and a sentence of 'Sue is missing her house' were used for recording and for observing of speech pattern. There were five mandibular positions observed in this study, that is, mandibular rest position, 'ssi', 'her', 'ha', and 's' speech position. In each position, slant and A-P distance in sagittal plane, vertical distance and lateral distance in frontal plane were measured. Amount of the mandibular rotational torque movement were measured at 'her', 'ha' speech position and for all through speech movement. Centric relation splint(CRS) was placed in both groups, but anterior or posterior bite plane were placed in normal subjects only. Data collected were processed and analysed by SPSS windows program. The results of this study were as follows : 1. Mandibular positions in both groups were not different before adaptation, with CRS, and after removal, but total amount of the mandibular rotational torque movement was greater in patients. 2. Mandible was slightly placed anteriorly with CRS at 'her' and 'ha' speech position in patients, but was placed anteriorly at all the five positions in normal subjects. 3. Difference with type of occlusal appliance in normal subjects were noted only for vertical distance at 'ssi' and 'ha' speech position, and the distance with CRS were more than that with posterior bite plane. 4. Mandibular rotational torque movement at 'her' and 'ha' speech position was greater in patients, but the difference was disappeared after appliance removal. And the torque movement was greater at 'ha' speech position than that at 'her' speech position in frontal plane. It could be concluded that the adaptation of occlusal appliance showed a tendency to locate the mandible anteriorly during speech in both groups, but did not affect total mandibular rotational torque movement which was greater in patients.

• Clinical and Experimental Reproductive Medicine
• /
• v.25 no.2
• /
• pp.109-113
• /
• 1998

Objective: To ananlyze the direct effect of nitric oxide (NO), generated from sodium prusside (SNP) on the embryo developments in reproductive process. Design: Ova from mouse were treated to allow fertilization in in vitro culture. And the samples of fertilized ova were alloted into five alliqutos. Each alliquot was cultured in media treated with either concentration at 0 (n=92), $25{\mu}M$ (n=84), $50{\mu}M$ (n=80), $100{\mu}M$ (n=77), $500{\mu}M$ (n=54) of SNP. Main Outcome Measure: Rates of embryonal cell cleavages, viability and cell morphology were assessed during in vitro fertilization and culture. Results: As analyse the cell cleavage at 24 hours after in vitro culture of fertilised egg in variuos NO concentration, all of egg cells of each alliquot were developed to $2\sim4$ cell stage. But the alliquot of egg cells treated with $50{\mu}M$, which were totally degenerated. And also all embryonal cells of each alliquot were developed to 8 cell stage and morula stage on culture continuosly. And the embryonal cells of each alliquot were analysed at 24 and 48 hours following the in vitro culture. The rates of cell fragmentation and fusion were $4.2{\pm}3.4%$ in control group which is not treated with NO, while experimental groups was high, as rated $23.4{\pm}6.2%$ in $25{\mu}M$, $28.2{\pm}5.7%$ in $50{\mu}M$ and $32.1{\pm}6.4%$ in $100{\mu}M$ concentration of NO. Accordingly the rate of abnormal morphology of embryonal cell in control was lower significantly than that in each alliquot of experimental groups (p<0.05). And the degenerated rates of embryonal cells were 0% in control, $17.8{\pm}6.7%$ in $25{\mu}M$, $23.6{\pm}4.7%$ in $50{\mu}M$ and $26.8{\pm}11.2%$ in $100{\mu}M$ at 8 cells and morula on culture of 48 and 72 hours. On the examination of embryonal cells developed to blastocyst through in vitro culture, the rates of degenerated cells were $16.8{\pm}7.2%$ in control, $37.5{\pm}6.2%$ in $25{\mu}M$, $73.4{\pm}4.6%$ in $50{\mu}M$, 100% in $100{\mu}M$. Conclusion: This results suggeted that the NO in any concentrations is harmful on embryos in view of morphology as well as viability of cell, and the toxicity of NO on embryo is stronger at condition in higher concentration of NO.