• Korean Journal of Microbiology
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• v.36 no.3
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• pp.228-235
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• 2000

Until now, it was diIliculi to overproduce lignin peroxidase(LiP) fiom Pl~anemchaete ch~ysosporium since the lack of optimized growth conditions. In this paper, we optimized the LIP production conditions and monitored LIP isozyines of fl chqsospoi.ium PSBL-1. The optimized condition includes sponge matrix support, no addition of $MnSO_4$, excess addition of niixogen source(48 inM diarmnonium), and addition of stabilizer(2 mM verakyl alcohol). Finally we obtained Lip activity of 1,800 unitsll. HI isozyne was overproduced when inyceliuin was cultivated in media containing $Mn^{2+}$ (2.73 inM) and excess nitrogen(48 11d4 diannnonium). Three azo dyes(acid yellow 9, congo ued, orange IT; each concenimtion of50 $\mu$M) we1-e rapidly decolorized within 2 inins by 0.4 un~t or Lip.

• Parasites, Hosts and Diseases
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• v.31 no.4
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• pp.353-362
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• 1993

The present study was carried out to investigate the localization and isozyme patterns of acid phosphatase and alkaline phosphatase in metacercariae and in adults of F. seoulensis by enzyme-histochemistry method and electrophoresis. Acidphosphatase showed a strong activity at pH 5 in the intestinal caecum of adults, but showed no reactions in the nonsubstrate control and in the inhibitor-treated control. Alkaline phosphatase showed a strong activity at pH 8 in the intestinal caecum and the tribocytic organ of adults, and in the intestinal caecum and in the genital anlagen of metacercariae. In non-denature PAGE, ten bands of protein fraction from the extracts of metacercariae and twenty-two bands from adults were detected. In denature PAGE, two protein bands having molecular weights of 192 kDa and 123 kDa were detected in the metacercariae, but absent from adult stage. In adults, protein fractions of 27.5 kDa, 24.5 kDa, 21.4 kDa, 18 kDa, 16 kDa and 15 kDa were detected. In non-denature PAGE, isozymes of acid phosphatase showed the most strong activity at pH 5, whereas no activity was shown at pH 2 and pH 7. One isozyme 85 kDa, 73 kDa and 62 kDa) in adults.

• Biomedical Science Letters
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• v.30 no.2
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• pp.81-85
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• 2024

Among all subjects who participated in the survey in the 9th 1st year (2022), 2,448 men (44.0%) and 3,166 women (56.0%) aged 12 or older were identified to determine oral care behavior according to orthodontic treatment experience. The purpose was to provide basic data on the oral care of patients wearing orthodontic appliances. 1. When it comes to brushing teeth, brushing before going to bed was the highest. Regarding the use of oral care products, 3,965 people (69.1%) said they 'do not use dental floss', 4,064 people (73.2%) said they 'do not use interdental brushes', and 4,064 people (73.2%) said they used oral rinse. 'I don't do it' was the highest at 4,417 (77.7%), and electric toothbrushes were the highest at 'I don't do it' at 5,241 (93.6%). 2. In terms of the effect of orthodontic treatment experience on toothbrushing time, with orthodontic treatment experience, toothbrushing after breakfast was 0.598 times more likely (P<0.001), and toothbrushing after lunch was 1.482 times more likely (P<0.001). Toothbrushing after dinner was 0.805 times higher (P=0.049), and toothbrushing before going to bed was 1.794 times higher (P<0.001), which was statistically significant. 3. When having orthodontic treatment experience, dental floss was found to be used 1.434 times more often (P=0.002), interdental brushes were used 1.464 times more times (P<0.001), and oral rinses were found to be used 1.457 times more times (P=0.002), which was statistically significant.

• Korean journal of applied entomology
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• v.30 no.3
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• pp.187-195
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• 1991

This study was carried out to acquire some basic biological informations on the granulosis virus (GV) of Pieris rapae and Pieris brassicae. Purified fractions of GV capsules in an sucrose density gradient centrifugation yielded on homogenous and sharp peak without a shoulder. Electron microscopy revealed that GV capsules were mostly ovalglove in shape. P. rapae and P. rapae GV isolated from P. rapae comprised granules($396\pm38\times238\pm25nm$ for P. rapae GV. $375\pm40\times255\pm28nm$ for P. brassicae GV) which contained single virus particle. The virus particles were 250- $275{\times}63$ -73nm for P. rapae GV and 243-250 $\times$ 63-75nm for P. brassicae GV containing a nucleocapsid 225 $\times$ 31nm for P. rapae, 225 $\times$ 29nm for P. brassicae within an envelope. The virulent difference between the two viruses was very small in their virulence for P. rapae larvae showing the $LC_{50}$( -log) with 5.5673 for P. rapae GV and 5.8104 for P. brassicae GV. Also the $LT_{50}$ of the 3rd instar P. rapae larvae against $10^{-6}$ inoculum was 8.17 days for P. rapae GV and 7.16 days for P. brassicae GV.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.73-73
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• 2003

This study was carried out to evaluate the utilization of fermented skipjack tuna viscera (FSTV) in the diet for juvenile abalone Haliotis discus hannai. Lactobacillus bulgaricus was used for fermentation of skipjack tuna viscera. Eight isonitrogenous (about 30% crude protein) diets were formulated to include different levels (0%, 10%, 20% and 30%) of FSTV as a replacer of either dietary fish meal or soybean meal. Three replicate groups of abalone were fed the experimental diets containing different levels of FSTV for 7 weeks. The inclusion of FSTV up to 30% in fish meal-based diet had no significant effect on survival, body weight, shell growth, and proximate composition of abalone (P>0.05). Weight gain of abalone fed the diet substituting 10% FSTV for soybean meal was not significantly different to that of abalone fed the control diet, however this value decreased in abalone fed the 20% and 30% FSTV (P<0.05).The contents of crude protein and lipid of soft body in abalone fed soybean meal-based diets were significantly affected by dietary FSTV level (P<0.05). The results of this study indicate that FSTV can be used as a partial substitute protein source for fish meal or soybean meal in the formulated diet for juvenile abalone.

• Food Science of Animal Resources
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• v.37 no.3
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• pp.385-391
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• 2017

Our study analyzed the carcass properties of 170 Korean native pigs in relation to carcass weight and back-fat thickness to provide general data for the production and distribution of high quality pig meat. The 70-74 kg group showed highest yield (73.41%). The ${\geq}80kg$ group showed the highest thickest back-fat (24.13 mm) (p<0.05). The ${\geq}80kg$ group showed the best quality grade (1.00). Back-fat thickness showed significant differences in the weight among groups (p<0.05). The ${\geq}25mm$ group showed the highest carcass weight (75.93 kg). The thickest back-fat group (${\geq}25mm$) showed the highest yield (73.03%). There were significant differences in back-fat thickness among groups (p<0.05), and the ${\geq}25mm$ group showed the highest thickness back-fat (27.60 mm). We found a strong positive correlation between carcass weight and back-fat thickness (r=0.346) as well as meat quality grade (r=0.739). Backfat thickness had a relatively strong positive correlation with meat quality grade (r=0.444). Therefore, there are required to manage the breeding through selection of excellent native species for increasing their carcass weight and enhance meat quality.

• Journal of Korean Clinical Health Science
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• v.2 no.1
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• pp.17-23
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• 2014

Purpose. This study was to investigate the relationship between the palpebral fissure size and the exophthalmos in a population of Korean adult women subjects with normal eye status. Methods. From November 2012 to September 2013, The authors analyzed the morphological values in 73 Korean adults population. The test was performed using the Hertel exophthalmometer and verner califer. Analysis of subjects data were recorded using the spss version 20.0 a statistical program (IBM Co, Armonk, NY USA). Results. The average age of the 73 subjects (146 eyes) was (21.219+/-0.261)mm. the average value of palpebral fissure was (25.110+/-1.646)mm in width and (8.096+/-1.464)mm in height. The average value of exophthalmos was (15.002+/-1.922)mm. There was a significant relationship(P>0.05) in the average size between the width and height in palpebral fissure. Also, there was a significant relationship(P>0.05) in the average size between the palpebral fissure width and exophthalmos. On the other hand, there was a significant relationship(P>0.01) in the average size of palpebral fissure height and exophthalmos. Conclusions: In this study, these results suggested that the palpebral fissure and exophthalmos had a significant relationship in the average value of size in Korean adult women population.

• Microbiology and Biotechnology Letters
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• v.26 no.1
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• pp.40-44
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• 1998

A Penicillium strain which produces $eta$-galactosidase with high transgalactosylation activity, was isolated from soil and registered as Penicillium sp, KFCC 10888. When $eta$-galactosidase from Penicillium sp. KFCC 10855 reacted with 40% lactose, transgalactosylation ratio reached up to 70% at the 73% conversion of initial lactose. The biosynthesis of the enzyme in Penicillium sp. KFCC 10888 was not induced by lactose. The soybean meal was an effective component of the culture medium. The optimum pH and temperature for transgalactosylation were 4.0 and 55$^{\circ}C$, respectively. The production of galactooligosaccharides was in proportion to the initial lactose concentration. When the enzyme reacted with 40% lactose (pH 4.0) at 55$^{\circ}C$, the concentration of galactooligosaccharides increased up to 40% of total solid concentration.

• The Korean Journal of Food And Nutrition
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• v.28 no.3
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• pp.458-464
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• 2015

In this study, the total polyphenol content, electron donating ability (EDA) and inhibitory activity of glutathione S-transferase (GST) of freeze-dried Citrus unshiu extracts were examined. The Citrus unshiu extracts was obtained from four solvents such as ethyl acetate, acetone, methyl chloride and methanol, to evaluate its functional properties. Total polyphenol contents were measured in the two different extracts, and the extracts were screened for their potential antioxidant activities using tests such as electron donating ability (EDA), glutathione S-transferase (GST). The total polyphenol contents of Citrus unshiu extracts were $928.48{\pm}1.19{\mu}g\;GAE/mL$ in ethyl acetate (EA), $886.03{\pm}0.44{\mu}g\;RE/mL$ in acetone (AC), $413.08{\pm}1.39{\mu}g\;GAE/mL$ in methylene chloride (MC), $12,648.60{\pm}0.56{\mu}g\;GAE/mL$ in methanol (MeOH), respectively. Also, the total polyphenol contents of EtOH Citrus unshiu extracts were $664.64{\pm}0.74{\mu}g\;GAE/mL$ in EA, $702.67{\pm}0.85{\mu}g\;RE/mL$ in AC, $429.64{\pm}0.61{\mu}g\;GAE/mL$ in MC, $16,108{\pm}0.73{\mu}g\;GAE/mL$ in MeOH, respectively. The total polyphenol contents were significantly difference (p<0.05) between the solvents. The electron donating ability of Citrus unshiu extracts were $62.80{\pm}0.36%$ in EA, $97.43{\pm}0.51%$ in AC, $52.20{\pm}0.30%$ in MC, $97.63{\pm}0.46%$ in MeOH, respectively. Also, the electron donating ability of EtOH Citrus unshiu extracts were $51.49{\pm}0.26%$ in EA, $63.17{\pm}0.31%$ in AC, $67.68{\pm}0.55%$ in MC, $96.18{\pm}0.41%$ in MA, respectively. The electron donating ability were significantly difference (p<0.05) between the solvents. The inhibitory activity of glutathione S-transferase in Citrus unshiu extracts were $76.22{\pm}0.65%$ in EA, $31.73{\pm}0.48%$ in MC, $97.48{\pm}0.56%$ in MeOH, respectively. Also, inhibitory activity of glutathione S-transferase in EtOH Citrus unshiu extracts were $75.54{\pm}0.55%$ in EA, $73.53{\pm}0.38%$ in MC, $48.70{\pm}0.46%$ in MeOH, respectively. The inhibitory activity of glutathione S-transferase were significantly difference (p<0.05) between the solvents. These results indicated that the Citrus unshiu extracts is a high-valued food ingredient and the extraction with methanol will be useful as a nutritional source with natural antioxidant activities. Considering high consumer demand beneficial health effects, Citrus unshiu extracts can be utilized to develop functional food health- promoting and natural antioxidant agents.

• Journal of the Korean Home Economics Association
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• v.20 no.4
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• pp.205-223
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• 1982

This study was devised to investigate the juveniles' psychological distance to their parents and related variables. 456 respondents, consisted of 116 middle school boys, 101 high school boys, 114 middle school girls and 125 high school girls, were sampled. The psychological distance was measured by the semantic differential method, and the statistical data were verified by the analysis of variance through computer system. The main results are found as follows. 1. The average mark of the juveniles' psychological distance to their parents was 54.73(78.19%)/70(100%) and the mark of high school girls was highest and high school boys lowest. 2. The variables influencing on the juveniles' psychological distance to their parents were varied buy the groups. 1) Middle school boys; home atmosphere(p＜.001), social and economical status of home (S.E.S; p＜.01), number of intimate friends(p＜.05), rearing attitudes of parents(p＜.05). 2) High school boys; home atmosphere(p＜.001), age of father(p＜.05), protection of mother(p＜.05) 3) Middle school girls; home atmosphere(p＜.001), rearing attitudes of parents (p＜.001), S.E.S of home(p＜.001).