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Efficacy of Assisted Hatching by Laser in Human IVF-ET Program (체외수정 및 배아이식술에서 레이저를 이용한 보조부화술의 효용성에 대한 연구)

  • Lee, Jung-Hyun;Han, Ji-Eun;Kim, You-Shin;Won, Hyung-Jae;Cho, Chung-Hyun;Kwak, In-Pyung;Eum, Jin-Hee;Park, Eun-A;Choi, Yoon-Jung;Lee, Dong-Ryul;Yoon, Tae-Ki
    • Clinical and Experimental Reproductive Medicine
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    • v.35 no.3
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    • pp.193-202
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    • 2008
  • Objective: To evaluate efficacy of assisted hatching by laser (AHL) and acidified Tyrode solution (AHA) in selected groups of IVF-ET patients who have a poor prognosis. Methods: From February 2006 to September 2006, total of 328 IVF-ET cycles with advanced female age (${\geq}38$ years), recurrent implantation failure (${\geq}3$ cycles), thick zona (${\geq}17{\mu}m$), and/or poor quality of embryo were randomly divided into assisted hatching by acidified Tyrode solution (AHA, n=180) and the assisted hatching using the ZILOS-tk laser (AHL, n=148) groups. Clinical outcomes were analyzed and compared between AHA and AHL group based on the patient characteristics. Results: In all AHL and AHA group, there were no significant differences in pregnancy (42.6%, 63/148 vs. 33.3%, 60/180) and implantation rates (17.4%, 82/470 vs. 16.0%, 89/556) However, in advanced female age group (Group 1), pregnancy (37.0%, 20/54 vs. 18.7%, 14/75) and implantation rates (14.4%, 23/160 vs. 7.1%, 15/210) in AHL group were significantly (p<0.05) higher than those of AHA, although there was no difference in patient parameters of both groups. And, the clinical outcome of groups with recurrent implantation failure (Group 2), thick zona pellucida (Group 3) and poor quality embryo (Group 4) were improved in AHL compared to those of AHA: 43.8% (21/48) and 31.6% (25/79) in Group 2, 43.8% (32/73) and 34.1% (28/82) in Group 3, 25.0% (7/28) and 14.6% (6/41) in Group 4, but no significance. Conclusion: The AHL improved the pregnancy and implantation rates in patients with advanced female age and recurrent implantation failure when compared to outcomes achieved from AHA. Therefore, this AHL technique may be a efficient and safe method for patients with poor prognosis.

Analysis of Chemical Components of Korean Loquat (Eriobotrya japonica Lindl.) Fruit (국내산 비파 열매의 화학적 성분 분석)

  • Lee, Boo-Yong;Park, Eun-Mi;Kim, Eun-Jeong;Choi, Hee-Don;Kim, In-Hwan;Hwang, Jin-Bong
    • Korean Journal of Food Science and Technology
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    • v.28 no.3
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    • pp.428-432
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    • 1996
  • The chemical components of Korean loquat (Eriobotrya japonica Lindl.) fruit were analysed. Approximate compositions of loquat flesh and seed were as follows. respectively crude lipid 0.53% and 0.83%, crude protein 0.05% and 5.27%, crude fiber 3.46% and 3.49%, crude ash 3.24% and 2.78%, carbohydrate 92.72% and 87.63% Soluble solids content, pH and acidity (citric acid) of loquat flesh juice were $12^{\circ}Bx$ by saccharometer, 4.43 and 0.18%, respectively. Free sugar compositions of loquat flesh and seed extracts $(3^{\circ}Bx)$ were as follows, respectively; fructose 0.77% and 0.31%, glucose 0.73% and 0.79%, sucrose 0.52% and 0.19%, ribose and 0.56%, Loquat flesh contained Glu 336.72 mg%, Asp 251.06 mg%, Arg 30.90 mg% and Lys 5.26 mg% Loquat seed contained Glu 448.23 mg%, Asp 335.63 mg%, lle 44.20 mg% and His 37.89 mg%, Potassium (k) contents of loquat flesh and seed were 32627.95 mg% and 28936.28 mg% in total amount of crude ash, while vitamin A and C of loquat flesh and seed were not detected. Composition of major lipid of loquat fruit seed oils fractionated by silicic acid was neutral lipids 43.78%, glycolipids 12.32% and phospholipids 43.90%, Fatty acid compositions of loquat seed lipid extracted by chloroform-methanol (2 : 1) were as follow; palmitic acid 23.72%, stearic acid 3.815, oleic acid 8.55%, linoleic acid 54.29% and linolenic acid 9.63%, Neutral lipids consist of palmitic acid 28.89, stearic acid 6.80%, oleic acid 11.07%, linoleic acid 40.67% and linolenic acid 12.58%, Glycolopids cinsist of palmitic acid 13.21%, stearic acid 4.56%, oleic acid 6.53%, linoleic acid 64.92% and linolenic aicd 10.77% Phospholipids consist of palmitic acid 30.95%, stearic acid 3.40%, oleic acid 9.09%, linoleic acid 48.45% and linolenic acid 8.10%.

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Quality Characteristics and Antioxidant Activity of Vinegar Supplemented Added with Akebia quinata Fruit during Fermentation (으름 열매 식초의 품질특성 및 항산화 활성)

  • Lee, Eun-Kyoung;Kwon, Woo-Young;Lee, Ji-Won;Yoon, Jin-A;Chung, Kang-Hyun;Song, Byeong Chun;An, Jeung Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.8
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    • pp.1217-1227
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    • 2014
  • This study investigated the physicochemical properties and antioxidant activity of vinegar added with different levels (0%, 1%, 3%, 5%, and 7%) of Akebia quinata fruit during two-step fermentation. The physicochemical properties of vinegar evaluated were pH, total acidity, alcohol, and total sugar and amino acid contents. The antioxidant activities were based on ABTS radical scavenging activity, SOD-like activity, and reducing power. During alcohol fermentation, total acidity and alcohol contents of vinegar increased, but total sugar contents decreased. During acid fermentation, total acidities of vinegar increased. Vinegar added with 7% A. quinata fruit showed the highest total sensory score. Total polyphenol contents of vinegar added with 0% and 1% A. quinata fruit were not significantly different. However, vinegar added with 3, 5, and 7% A. quinata fruit showed significantly higher total polyphenol contents of 136.6, 381.59, and 415.35 mg/100 g, respectively, after 13 days of fermentation. Further, total flavonoid contents of vinegar added with 0~7% A. quinata fruit significantly increased to 21.73, 15.79, 15.15, 26.19, and 26.87 mg/100 g, respectively, after 13 days of fermentation. In addition, tannin contents of vinegar added with 0~7% A. quinata fruit significantly increased to 0.2042, 0.2004, 0.1255, 0.1384, and 0.1255 mg/100 g, respectively, after 13 days of fermentation. Moreover, ABTS radical scavenging activities of vinegar added with 0~7% A. quinata fruit significantly increased to 5.87, 12.59, 25.63, 34.02, and 35.25, respectively, after 13 days of fermentation at a concentration of 5 mg/mL. Additionally, SOD-like activities of vinegar added with 0~7% A. quinata fruit significantly increased to 8.22, 17.49, 16.86, 16.89, and 15.68%, respectively, after 13 days of fermentation. Reducing power of 7% A. quinata fruit was 0.527 after 1 day and 1.539 at the end of fermentation. Our results demonstrate that antioxidant activity significantly increased during fermentation according to the content of A. quinata. Further, the total polyphenol, flavonoid, and tannin contents were shown to be closely related with antioxidant activities. Thus, A. quinata could be effectively used as a vinegar and functional food material based on its antioxidant activity.

Physicochemical and Sensory Characteristics of Sesame Oils Manufactured in Korea, Japan and China (한국(韓國), 일본(日本), 중국(中國) 삼개국(三個國) 참기름의 이화학적(理化學的) 특성(特性) 및 궁능적(宮能的) 특성(特性))

  • Kim, Hyeon-Wee;Lee, Min-Jung;Kim, Ki-Hong
    • Proceedings of the EASDL Conference
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    • 2004.10a
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    • pp.107-129
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    • 2004
  • Sesame oil has been popular for hundreds of years in Korea because of its pleasant flavor and health benefits and has been studied for its antioxidant properties and flavor preferences attributed to its manufacturing methods. The objective of this study was to investigate the qualitative properties of six commercial sesame oils (3 Korean, 2 Japanese, 1 Chinese), The fatty acids in the oil are composed of two main acids oleic acid and linoleic acid with a P/S ratio of 4.99${\sim}$5.73. Of the tocopherol isomers, ${\gamma}$-toc ranged from 23.14 to 34.85mg/100g. Lignan such as sesamin(322.91${\sim}$689.39ppm) and sesamolin (62.19${\sim}$289.82 ppm) is found predominantly in sesame oil. Sesamol (8.52${\sim}$51.21 ppm) was significantly different depending on manufacturer, observed as greatest in the Korean and least in the Japanese products. The induction period was longest in order of the Korean, Chinese, and then Japanese product. The red and yellow values in Lovibond color were highest in the Korean and lowest in the Japanese product. The major volatile compounds (in order of content) were pyrazines, phenols, aldehydes, and then furans and contained a small amount of pyrroles, thiazoles and indoles. The levels of total volatiles were greatest in the Korean and least in the Japanese product. The most abundant volatiles in the Korean product were pyrazines, whereas phenols were higher in the Chinese product compared to the others. From these results, the relationships among pyrazines, sesamol, yellowness and induction period showed positive, respectively. In sensory evaluation, Korean panelists preferred, in order, the Korean, Japanese, and then the Chinese product in strength of and preference for the sesame flavor, also ranking it best in overall acceptance. Japanese panelists found similarities in the Korean and Japanese products and gave an equal level of preference for the sesame flavor and overall acceptance. On the other hand, Chinese panelists preferred the Japanese product in strength and sesame flavor rating it best on overall acceptance.

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Analysis of Behavioral Characteristics of Broilers by Feeding, Drinking, and Resting Spaces according to Stocking Density using Image Analysis Technique (영상분석기법을 활용한 사육밀도에 따른 급이·급수 및 휴식공간별 육계의 행동특성 분석)

  • Kim, Hyunsoo;Kang, HwanKu;Kang, Boseok;Kim, ChanHo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.12
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    • pp.558-569
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    • 2020
  • This study examined the frequency of a broiler's stay in each area as stock density using an ICT-based image analysis technique from the perspective of precision livestock farming (PLF) according to the increase in the domestic broiler farms to understand the normal behavior patterns of broilers by age. The broiler was used in the experimental box (3.3×2.7 m) in a poultry house in Gyeonggi province. The stock densities were 9.5 birds/㎡ (n=85) and 19 birds/㎡ (n=170), respectively, and the frequency of stay by feeding, water, and rest area was monitored using a top-view camera. The image data of three-colored-specific broilers identified as the stock density were acquired by age (12, 16, 22, 27, and 29 days) for six hours. In the collected image data, the object tracking technique was used to record the cumulative movement path by connecting approximately 640,000 frames at 30 fps to quantify the frequency of stay in each area. In each stock density, it was significant in the order of the rest area, feeding, and water area (p<0.001). In 9.5 birds/㎡, it was at 57.9, 24.2, and 17.9 %, and 73.2, 16.8, and 10 % in 19 birds/㎡. The frequency of a broiler's stay could be evaluated in each area as the stock density using an ICT-based image analysis technique that minimizes stress. This method is expected to be used to provide basic material for developing an ICT-based management system through real-time monitoring.

Effects of Temperature on the Development and Reproduction of Phaedon brassicae Baly (Coleoptera: Chrysomelidae) (좁은가슴잎벌레의 발육과 생식에 미치는 온도의 영향)

  • Jeong Joon Ahn;Kwang Ho Kim;Hong Hyun Park;Gwan Seok Lee;Jeong Hwan Kim;In-Hong Jeong
    • Korean journal of applied entomology
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    • v.62 no.4
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    • pp.315-323
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    • 2023
  • The brassica leaf beetle, Phaedon brassicae Baly (Coleoptera: Chrysomelidae), is one of the important pests infesting cruciferous vegetables. In order to understand the biological characteristics of the insect, we investigated the effects of temperature on development of each life stage, adult longevity and fecundity of P. brassicae at four constant temperatures of 15, 20, 25 and 27.5℃ for immature life stage and five constant different temperatures of 10, 15, 20, 25 and 27.5℃ for adult stage. Eggs and larvae successfully developed next life stage at temperature tested. The development period of egg, larva, and pupa decreased as temperature increased. Lower developmental threshold (LDT) and thermal constant (K) were calculated using linear regression as 8.7℃ and 344.73DD, respectively. Lower and higher threshold temperature (TL and TH) from egg to adult emergence were estimated by Briere function as 5.3℃ and 40.4℃, respectively. Adults produced eggs at the temperature range between 10℃ and 27.5℃, and showed an estimated maximum number, ca. 627.5 eggs at 21.7℃. Adult oviposition models including aging rate, age-specific survival rate, age-specific cumulative oviposition, and temperature-dependent fecundity were constructed. Temperature-dependent development models and adult oviposition models would be useful components to understand the population dynamics of P. brassicae and to establish the strategy of integrated pest management in cruciferous crops.

Production of DHA-Rich Meats and Eggs from Chickens Fed Fermented Soybean Meal by Marine Microalgae(Schizochytrium mangrovei MM103) (해양미세조류(Schizochytrium mangrovei MM103)를 이용한 발효 대두박 급이에 따른 DHA 다량 함유 육계와 계란의 생산)

  • Jeong, Woo-Cheol;Lee, Jeong-Yeoul;Kim, Sang-Ho;Lee, Sang-Jin;Choi, Byeong-Dae;Kang, Seok-Joong
    • Korean Journal of Poultry Science
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    • v.35 no.3
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    • pp.255-265
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    • 2008
  • The objective of this study was to evaluate the docosahexaenoic acid(DHA) levels of meats and eggs from chickens which were fed fermented soybean meal(FSM) by marine microalgae(Schizochytrium mangrovei MM103). The diets contained different amounts of FSM at 0, 3, 5 and 10%. DHA content of carcass was increased with dietary FSM. DHA amounts in the breast meat were higher in the 10% FSM diet(2.21%) than the 5%(1.65%) and 3%(1.18%) FSM, and similar results were observed in the leg meat(10% FSM: 2.21%; 5% FSM: 1.65%; and 3% FSM: 1.18%, respectively) and in eggs(10% FSM: 2.02%; 5% FSM: 1.22%; and 3% FSM: 0.73%). The level of n-3 polyunsaturated fatty acids such as DHA(22:6n-3) in the FSM treatment was significantly higher than those of the other groups(P<0.05). The results demonstrated that FSM by marine microalgae could be used to enhance DHA amounts in chicken meats and eggs.

Association of waist circumference with muscle and fat mass in adults with a normal body mass index

  • Lee, Gyeongsil;Choi, Seulggie;Park, Sang Min
    • Nutrition Research and Practice
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    • v.15 no.5
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    • pp.604-612
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    • 2021
  • BACKGROUND/OBJECTIVES: We aimed to investigate the association of waist circumference (WC) with body composition among individuals with a normal body mass index (BMI) to distinguish muscle and fat mass, as both affect health differently. SUBJECTS/METHODS: We analyzed dual-energy X-ray absorptiometry data (derived from the Korean National Health and Nutrition Survey, which includes information on fat and lean mass) of 7,493 adults with a normal BMI. Subjects were categorized into four groups of increasing WC. The fourth group was defined as being centrally obese. Each number of subjects are as follows: 1,870, 695, 231, and 39 among men and 3,054, 1,100, 406, and 98 among women. We conducted a sex-stratified linear regression analysis of body composition according to WC group after adjustments for covariates. RESULTS: We observed a positive association of body fat with increasing WC in both men and women (all P for trend: < 0.001). The adjusted mean values for percent body fat with 95% confidence intervals (CIs) according to the four WC groups in ascending order were 17.8 (17.5-18.3), 21.0 (20.6-21.5), 22.1 (21.5-22.8), and 25.1 (24.2-26.1) in men and 29.7 (29.4-30.0), 32.0 (31.6-32.3), 32.9 (32.4-33.4), and 34.7 (33.2-36.1) in women. However, there was an inverted J-shaped association between muscle mass and WC. The fourth group had a higher percent body fat and lower muscle mass than other groups. The adjusted mean values for appendicular skeletal muscle mass index (kg/m2) with 95% CIs according to the four WC groups in ascending order were 7.55 (7.51-7.59), 7.62 (7.56-7.68), 7.65 (7.56-7.74), and 7.22 (7.04-7.41) in men and 5.83 (5.80-5.85), 5.96 (5.92-6.00), 6.03 (5.96-6.10), and 5.88 (5.73-6.03). CONCLUSIONS: There was a positive association between body fat and WC among individuals with normal BMI; conversely there was an inverted J-shaped association between lean body mass and WC. Our findings support the WC measurement should be included in obesity evaluations for adults with a normal BMI.

External Beam Irradiation for Squamous Cell Carcinoma of the Maxillary Sinus (상악동 편평세포암종에서의 방사선치료)

  • Kim Tae-Hyun;Yoon Won-Sub;Yang Dae-Sik;Kim Chul-Yong;Choi Myung-Sun
    • Radiation Oncology Journal
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    • v.21 no.1
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    • pp.19-26
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    • 2003
  • Purpose : Maxillary sinus cancers are usually locally advanced, and involve the structures around sinus, but the regional lymphatic spread is uncommon. Therefore, the local control of these cancers is important for their cure. We reviewed our experience of 55 patients with squamous cell carcinomas of the maxillary sinus, treated with radiation therapy, and looked for the role of radiation therapy in maxillary sinus cancers. Materials and Methods : Between November 1982 and October 1999, 55 patients with squamous cell carcinomas of the maxillary sinus underwent either radiation therapy only, or combined with surgery or with concurrent chemoradlation therapy. All patients were restaged according to the 1997 AJCC staging systems. The T classifications of the tumors of the patients were as follows 1.8$\%$ (1/55) for 72, 81.8$\%$ (45/55) for 73 and 16.4$\%$ (9/55) for 74. Thirteen patients were diagnosed with lymph node involvement. With the surgical procedures, 12 patients were managed by biopsy only, 21 were resected by FESS (funclienal endoscopic sinus surgery) and 22 by partial/medical/total rnaxillectomies. The details of the treatments were as follows 8 patients were treated with radiation therapy only, 17 with a combination of FESS and radiation therapy, 22 with a combination of a maxillectomy and radiation therapy, 4 with a combination of preoperative radiation therapy and surgery, and 4 with concurrent chemoradiation therapy. The mean follow-up for all patients was 25 months, ranging from 2.8 to 125 months. Results : The 4-year local control and survival rates for all Patients were 45.5 and 33.3$\%$, respectively. The 4-year local control and suHival rates, due to the extent of surgery, were as follows 32.1, and 21.4$\%$ for biopsy; 41.9, and 31.7$\%$ for FESS; and 56.8, and 52.7$\%$ for maxillectomy, respectively. Twenty-nine (52.7$\%$) patients were not cured, and of these 29 patients, 23 (79.3$\%$) patients had a iocal recurrence following treatment. Conclusions :This study has shown that the major failure sites following treatment to be the local regions, and that the completeness of surgery was important for improving the local control and survival of patients with squamous cell carcinoma of the maxillary sinus.

Changes in s-GOT and s-ALP Activities in the Korean Native Cows Within Estrus Cycle (한우(韓牛)의 성주기(性週期)에 따른 혈청(血淸) GOT 및 혈청(血淸) ALP 활성도(活性度)의 변동상에 관하여)

  • Jo, Chung Ho
    • Korean Journal of Veterinary Research
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    • v.21 no.2
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    • pp.167-170
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    • 1981
  • Changes in serum glutamic oxaloacetic transaminase (s-GOT) activities, serum alkaline phosphatase activities and serum total protein amounts were investigated on seven Korean native cows having normal estrus cycle of 18~24 days after normal parturition, dividing estrus cycle into estrus (0~1 days), metestrus (2~6 days), diestrus (7~16 days), proestrus (17~20 days). The results were as follows. 1. Serum GOT activities at estrus ranged from 73.5 to 121.5 Ku with a mean of $89.14{\pm}17.16$ Ku, at metestrus 57 to 89 Ku with a mean of $67.01{\pm}10.81$ Ku, at diestrus 54 to 89.5 Ku with a mean of $67.05{\pm}10.05$ Ku and at proestrus 53 to 112 Ku with a mean of $73.00{\pm}20.05$ Ku. The activities were significantly increased at the estrus comparing with other stages (P<0.01). 2. Serum ALP activities at estrus ranged from 8.0 to 10.4 K-Au with a mean of $8.74{\pm}0.83$ K-Au, at metestrus 6.5 to 9.2 K-Au with a mean of$7.74{\pm}0.76$ K-Au, at diestrus 5.6 to 9.0 K-Au with a mean of $7.67{\pm}1.13$ K-Au, at proestrus 6.2 to 9.3 K-Au with a mean of $7.12{\pm}1.18$ K-Au. The significance was not recognized among the stages of estrus cycle. 3. Serum total protein amounts at estrus ranged from 6.45 to 8.0g/10dl with a mean of $7.25{\pm}0.57/100dl$, at metestrus, 6.37 to 7.9g/100dl with a mean of $7.65{\pm}0.50g/100dl$, fat diestrus, 6.56 to 8.67g/100dl, with a mean of $7.53{\pm}0.55g/100dl$ and at proestrus 5.94 to 7.71g/100dl with a mean of $6.54{\pm}0.65g/dl$. There was not significance among the stages of estrus cycle.

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