• Asian Pacific Journal of Cancer Prevention
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• v.17 no.4
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• pp.1817-1821
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• 2016

Objectives: To observed the effects of ginsenoside -Rh2 (GS-Rh2) on proliferation and apoptosis of side population (SP) human gastric cancer SGC-7901 cells. Materials and Methods: SGC-7901 SP and Non-SP cells were sorted by flow cytometry and assessed using the cck-8 method. Expression of apoptosis-related proteins Bax and Bcl-2 of SP before and after the intervention was determined by Western-blotting. Results: It was found that the proliferation of SP was significantly faster than that of NSP (P<0.05). In addition, GS-Rh2 inhibited proliferation of gastric cancer SP cells, induced cell cycle arrest and cell apoptosis, and changed the expression of BAX/Bcl-2 proteins in a time-dependent and concentration-dependent manner (P<0.05). Conclusions: With increase of GS-Rh2 dose, GS-Rh2 gradually inhibit the proliferation of SGC-7901 SP cells, which have high proliferation rate, through G1/G0 phase arrest, followed by apoptosis which involves the up-regulation of Bax and the down-regulation of Bcl-2.

• Annals of Clinical Neurophysiology
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• v.5 no.1
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• pp.16-20
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• 2003

Background: Mobile phone has become a very popular device used in everyday our lives. However, the possible hazard to human body such as brain tumor has been proposed intermittently. This unwanted possibility was calmed down due to the absence of definite evidence of hazard. This study was performed to see the effect of mobile phone use on the brain temperature. Methods: In 20 volunteers, we performed 4 steps of temperature measuring procedure. Four steps are pre-use (S1, basal state), wire-phone (S2, conventional telephone), PCS phone (S3, using 1,750~1,900 MHz), cellular phone (S4, using 820 MHz) states. Brain temperatures were measured by radiothermometer at 10 sites (5 sites in each hemisphere) of the brain after 5minutes of telecommunication through the phones. The final data were compared using paired t-test. Results: In PCS phone user group (Average; $35.73708^{\circ}C$), brain temperature decreased (with statistical significance, p<0.05), compared to those of non-user group (Average; $35.9527^{\circ}C$) or conventional wire phone user group. In cellular phone user group (Average; $35.82155^{\circ}C$), brain temperature decreased slightly (without statistical significance, p>0.05) compared to those of non-user group (Average; $35.9527^{\circ}C$) or conventional wire phone user group (Average; $35.922^{\circ}C$). The temperature change was not limited to the mobile phone applied side but on both hemisphere of the brain. Conclusion: In conclusion, mobile phone (especially PCS phone) decreased brain temperature in both hemispheres without side-to-side temperature difference. In addition, this study suggests possibility of radiothermometer application to the study of electromagnetic wave effect and protection method research in the future.

• Molecular & Cellular Toxicology
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• v.3 no.1
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• pp.60-67
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• 2007

Gentamicin is a broad-spectrum aminoglycoside antibiotic used in the treatment of bacterial infection. Although side effects of gentamicin such as nephrotoxicity and ototoxicity have been investigated, the information on the hepatic effects of gentamicin is still limited. In the present study, gene expression profiles were analyzed in the liver of gentamicin treated mice using Affymetrix GeneChip$^{(R)}$ Mouse Expression 430A 2.0 Array. Totally, 400 genes were identified as being either up- or down-regulated over 1.5-fold changes (P<0.01) in the liver of gentamicin treated mice. Among these deregulated genes, 16 up-regulated genes mainly involved in transport (Kif5b, Pex14, Rab14, Clcn3, and Necap1) and 20 down-regulated genes involved in lipid and other metabolisms (Hdlbp, Gm2a, Uroc1, and Dak) were selected using k-means clustering algorithm. The functional classification of differentially expressed genes represented that several stress-related genes were regulated in the liver by gentamicin treatment. This data may contribute in understanding the molecular mechanism in the liver of gentamicin treated mice.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2007.11a
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• pp.129-139
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• 2007

Many modalities of treatment for acquired skin hyperpigmentation are available including chemical agents or physical therapies, but none are completely satisfactory. The ideal depigmenting compound should have a potent. rapid and selective bleaching effect on hyperactivated melanocytes, carry no short- or long-term side-effects and lead to a permanent removal of undesired pigment. acting at one or more steps of the pigmentation process. Depigmentation can be achieved by regulating (i) the transcription and activity of tyrosinase, tyrosinase related protein-1 (TRP-1), tyrosinase related protein-2 (TRP-2), and/or peroxidase; (ii) the uptake and distribution of melanosomes in recipient keratinocytes and (iii) melanin and melanosome degradation and turnover of pigmented keratinocytes. One of the interesting point for development of skin whitening agent is Mitf(Microphthalmia-associated transcription factor). Mitf belongs to the basic helix-loop-helix-zip family of trabscription factors and it is crucial as it regulates both melanocyte proliferation as well as melanogenesis and is the major regulator of tyrosinase and the related enzymes (TRPs), as well as many melanosome structural proteins such as pMel17. Recently, we developed MITF-down-regulating agents from natural and synthetic sources, which have anti-melanogenic effect on in vitro and in vivo. We suggested that potent MITF-down regulating agents might be used for skin whitening cosmeceuticals.

• Physical Therapy Rehabilitation Science
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• v.11 no.4
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• pp.526-534
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• 2022

Objective: The purpose of this study was to compare muscle activities of stroke patients and healthy participants during bimanual tasks. Design: A cross sectional study. Methods: A total of 25 participants (13 hemiparetic stroke patients and 12 healthy participants) were recruited. The muscle activities using electromyogram (EMG) during bimanual tasks were collected from the following muscles: extensor carpi radialis longus (ECRL), biceps brachii (BB), and triceps brachii (TB). The bimanual tasks included eight tasks consisted of (1) raising the wrists up and down, (2) supinating and pronating the palms, (3) touching the shoulder with fingertips, (4) drawing vertical dot, (5) reaching for a cup and bring it in to drink, (6) drawing a circle outward and (7) inward, and (8) grasping the fingers. The EMG data collected from the muscles of paretic and non-paretic sides of stroke patients and the average from both sides of healthy subjects were normalized and compared after calculating the percentage of maximal isometric voluntary contraction. Results: The ECRL, BB and TB of the paretic side of the stroke patients showed relatively greater muscle activity compared to the non-paretic side as well asaverage of the healthy subject duringall tasks (p<0.05). In addition, the ECRL showed the highest muscle activity during most of the tasks. All of the non-paretic side musclesfrom stroke patients showed higher muscle activity compared to those of healthy subjects. Conclusions: The current study showed that muscle activities of upper extremity varied between paretic and non-paretic sides of stroke patients during bimanual tasks. Interestingly, the non-paretic side muscle activities were also different from those of normal participants.

• Journal of dental hygiene science
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• v.7 no.3
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• pp.179-185
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• 2007

The purpose of this study targeted on high school student in the department of liberal arts, industry in Daegu metropolitan city, is to get basic data necessary for the development of dental educational program, to discern prevention and treatment of temporomandibular joint disorder by observing the situation temporomandibular joint disorder and contribution element, of relationship of school life stress The results are as follows.: 1. The percentage of occurring temporomandibular joint disorder in the high school resulted in a joint noise at 61.8% and joint dislocation 6.9%, sharp pain 47.5% at time of chewing. 29.8% at the time of the non-chewing, lockjaw 11.3%, a headache appeared at 40.4%.2. In the contribution factor of occurring temporomandibular joint disorder, the cause of joint noise was the clench one's teeth, lip and cheek clench, For the pain at the time of chewing clench one's teeth, one side chewing, over-chewing, lip clench, sideways sleeping showed the difference. (P < 0.01) For the pain at the time of non-chewing, clench one's teeth, bruxism, one side chewing, lip and cheek clench were similar, and for the lockjaw, clench one's teeth, bruxism, sideways sleeping showed the difference. The plum evil thing period at time of the fault writing that statistically showed the difference. For the headache, the contribution factors were the all bad habits mentioned above excluding one side sleeping.(P < 0.01, P < 0.05). 3. The rate of experiencing temporomandibular joint disorder by oral and maxillofacia was 13.4% in industrial department, and 19.6% in liberal arts. And for the factor of wound was that exercise 26.8%, others 24.4%, fall-down 19.5%. And for the industrial, exercise 44.4%, fall-down 22.2%, others 14.9%. The treatment experience appeared at 5.0% in industrial department, 2.9% in liberal arts. And for the medical institutions, liberal arts were dental clinic 50%, orthopedics 50%, and the industrial department orthopedics 40%, oriental medicine clinic 30%, dental clinic 30%. 4. In case of temporomandibular joint disorder, there were no difference by grades or educational background. And at the time of chewing or non-chewing showed similar difference.(P < 0.01). 5. Compared to stress in the high school, it generally showed higher in liberal arts than in industrial department due to school record. Its scope was $3.75{\pm}1.14$ in liberal arts, $3.01{\pm}1.23$ in industrial department. 6. The school record, school life, stress problems by teachers, chewing/non-chewing pain of temporomandibular joint disorder, joint noise had a similar correlation.(P < 0.01, < 0.05).

• Journal of the Korean Magnetic Resonance Society
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• v.9 no.1
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• pp.21-28
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• 2005

$^{11}B$ nuclear magnetic resonance (NMR) measurements have been performed to investigate electronic structures and vortex states of $M_gB_2$ superconductor. The central transition shows a narrow peak down to 25 K at 3.15 T. Below 25 K, an extra line starts to show up and dominates. The extra line is broad and asymmetric with a long tail in the high frequency side, which confirms that this originates from vortex pinning below the irreversibility temperature. From temperature evolution of the fraction and linewidth of the broad portion, temperature dependence of coherence length and penetration depth are extracted.

• The Plant Pathology Journal
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• v.26 no.4
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• pp.421-423
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• 2010

New sources of barley (Hordeum vulgare L.) resistant to spot blotch, caused by Cochliobolus sativus, are needed to provide effective resistance because of the rapid change pathotype patterns of C. sativus in fields. The purposes of our study were to develop a method to screen barley for resistance to spot blotch disease and then use this methodology to screen barley genotypes for resistance to the major virulent pathotype Pt4 in barley populations in Syria. A transparent tape method, in which a conidial suspension of C. sativus was dropped onto transparent tape and placed, treated-side down, on the second leaf surface of barley plants. Disease symptoms of fungus were easily detected on the leaves covered by the transparent tape after 48h of inoculation. The transparent tape method was repeatable and the disease scores obtained were correlated (r = 0.91, P = 0.001) with those obtained by the seedling assay. This method may be beneficial in various plant pathology breeding programs.

• Journal of Digital Convergence
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• v.16 no.4
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• pp.221-229
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• 2018

The purpose of this study was to identify the characteristics and side effects relevance of physically restrained elderly patients with dementia in the nursing hospitals. The data were collected from nursing care providers working at the 5 nursing hospitals with survey and analyzed 190 data using SPSS 21.0 program. The finding showed that age of '75 to 84' years occupied 58.3%, '17-24' hours of the total restrained time of the day 22.6%, night time 57.4% and no fall down history 54.2%. Age showed relevance with local edema and problem of joint construction(p<.05), specifically '85 and over' years having 25% occurrence in the problem of joint construction. Walking ability showed relevance with pressure ulcer, but in the patients who could walk it showed more frequency than in the patients who could not walk. The total restrained time of the day showed relevance with skin redness, local edema, problem of joint construction(p<.05). Based on this finding, it would be necessary to apply differential care policies according to the characteristics of patients when we care the restrained patients with dementia.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.6
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• pp.1843-1849
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• 2004

Conventional medicines have usually sorted to a number of treatments such asoperation, radiotherapy, and chemotherapy. The existing anti-cancer agents, designed to eradicate cancer cells, have strong toxicities, also with leading to harmful side effects. Recently, a number of researches on natural products have been actively carried out in efforts to develop new treatments that can decrease side effects or increase anti-cancer effects. We performed this study to understand the molecular basis underlying the antitumor effects of Pharbitis nil, and Plantago asiatica, which have been used for herbal medicinal treatments against cancers in East Asia. We analyzed the effects of these medicinal herbs on proliferation and on expression of cell growth/apoptosis related molecules, with using an AGS gastric cancer cell line. The treatment of Pharbitis nil dramatically reduced cell viabilities in a dose and time-dependent manner, but Plantago asiatica didn't. FACS analysis and Annexin V staining assay also showed that Pharbitis nil induce apoptotic cell death of AGS. Expression analyses via RT-PCR and Western blots revealed that Pharbitis nil didn't increase expression of the p53 and its downstream effector p21/sup wafl/, and that the both increased expression of apoptosis related Sax and cleavage of active caspase-3 protein. We also confirmed the translocation of Sax to mitochondria. Collectively, our data demonstrate that Pharbitis nilinduce growth inhibition and apoptosis of human gastric cancer cells, and these effects are correlated with down- and up-regulation of growth-regulating apoptotic and tumor suppressor genes, respectively.