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Comparison of Methane Production in Korean Native Cattle (Hanwoo) Fed Different Grain Sources (곡류 사료원별 육성기 한우 장내발효에 의한 메탄가스 배출량 비교)

  • Seol, Yong-Joo;Kim, Kyoung-Hoon;Baek, Youl-Chang;Lee, Sang-Cheol;Ok, Ji-Woun;Lee, Kang-Yeon;Hong, Seong-Koo;Park, Kyu-Hyun;Choi, Chang-Weon;Lee, Sung-Sil;Oh, Young-Kyoon
    • Journal of Animal Science and Technology
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    • v.53 no.2
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    • pp.161-169
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    • 2011
  • Methane production during anaerobic fermentation in the rumen represents an energy loss to the host animal and induces emissions of greenhouse gases in the environment. Our study focused on comparison in methane production from growing Korean native steers fed different grain sources. Six Hanwoo steers (BW = $180.6{\pm}3.1$ kg) were fed, on a DM basis (TDN 2.80 kg), 40% timothy and 60% barley concentrate (Barley) or corn concentrate (Corn), respectively, based on the Korean Feeding Standards. Each period lasted 18 days including a 14-day adaptation and a 4-day measuring times. The steers were in the head hood chamber system (one cattle per chamber) during each measuring time to measure heat and methane production per day. Different grain sources did not affect digestibilities of dry matter, crude protein, crude fiber, crude fat, NDF, ADF and nitrogen-free extract. The mean methane concentrations per day were 202.0 and 177.1 ppm for Barley and Corn, respectively. Methane emission averaged 86.8 and 77.7 g/day for Barley and Corn, respectively. Methane emission factor by maintenance energy requirement for the growing steers fed barley based concentrate was higher than the steers fed corn based concentrate (Barley vs. Corn, 31.7 kg $CH_4\;head^{-1}\;yr^{-1}$ vs. 28.4 kg $CH_4\;head^{-1}\;yr^{-1}$). Thus, methane conversion rate was 0.065 (6.5%) and 0.055 (5.5%) for Barley and Corn, respectively.

Chemical Composition and Fermentation Characteristics of Storage Sections of the Round Bale Silage of Fresh Rice Straw at Yonchon of Gyeonggi-do (연천지역에 있어서 생볏짚 원형곤포사일리지의 부위별 사료성분 및 발효품질)

  • Kim, Sang-Rok;Kim, Gon-Sik;Woo, Jae-Hoon;Lee, Jun-Woo;Sung, Kyung-Il
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.24 no.3
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    • pp.253-260
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    • 2004
  • The purpose of this study is to discuss the ways to evaluate the effectiveness of storage sections of the round bale silage of fresh rice straw (RS). This study evaluated, the changes of the fermentation characteristics and chemical composition of the different sectors of the RS after a certain period; a month later after the bailing, samples of three different RS (bale one, bale two, and bale three) were taken according to the three different sectors (top, center, and the bottom) of the bale. According to the findings of this study there was no significant difference in the RS's chemical composition content among the different sectors (top, center, or the bottom) of the bale. The possible reason for this is believed that on the basis of different sectors of the bale, the moisture (the water soluble nutrient) movement didn't occur and was locked in and couldn't escape, because the dry matter content of the rice straw was as high as $70\%$. After immediately harvesting the paddies, using the fresh rice straw to produce RS at the same time when the dry matter content is in between $26{\sim}40\%$, the chemical composition was found to be of a good quality. Accordingly, it is recommended that when the dry matter content is in between $35{\sim}45\%$, in order to produce RS with the fine chemical composition of 'Yonchon' region, bailing should be done simultaneously with the harvesting of paddies, before the first frost appears.

Petrology and petrochemistry of the Jurassic Daebo granites in the Pocheon-Gisanri area (포천 - 기산리 일대에 분포하는 쥬라기 대보화강암류의 암석 및 암석화학)

  • 윤현수;홍세선;이윤수
    • The Journal of the Petrological Society of Korea
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    • v.11 no.1
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    • pp.1-16
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    • 2002
  • The study area is mostly composed of Precambrian Gyeonggi gneiss complex, Jurassic Daebo granites, Cretaceous tonalite and dykes, and so on. On the basis of field survey and mineral assemblage, the granites can be divided into three types; biotite granite (Gb), garnet biotite granite (Ggb) and two mica granite (Gtm). They predominantly belong to monzo-granites from the modes. Field relationship and K-Ar mica age data in the surrounding area suggest that intrusive sequences are older in order of Gtm, Ggb and Gb. Gb and Ggb, major study targets, occur as medium-coarse grained rocks, and show light grey and light grey-light pink colors, respectively. Mineral constituents are almost similar except for opaque in Gb and garmet in Ggb. Gb and Ggb have felsic, peraluminous, subalkaline and calc alkaline natures. In Harker diagram, both rocks show moderately negative trends of $TiO_2$, MgO, CaO, $Al_2O_3$, $Fe_2O_3$(t), $K_2O$ and $P_2O_5$ as $SiO_2$ contents increase. Among them, $TiO_2$, MgO and CaO show two linear trends. From the trends and the linear patterns in AFM, Sr-Ba and Rb-Ba-Sr relations, it is likely that they were originated from the same granitic magma and Ggb was differentiated later than Gb. REE concentrations normalized to chondrite value have trends of parallel LREE enrichment and HREE depletion. One data of Ggb showing a gradually enriched HREE trend may be caused by garnet accompaniment. Ggb have more negative Eu anomalies than Gb, suggesting that plagioclase fractionation in Ggb have occurred much stronger than that in Gb. In modal (Qz+Af) vs. Op, Gb and Ggb belong to magnetite-series and ilmenite-series, respectively. From the EPMA results, opaques of Gb are magnetite and ilmenite, and those of Ggb are magnetite-free ilmenite or not observed. Bimodal distribution of magnetic susceptibility reveals two different granites of Gb (332.6 ${mu}SI$) and Ggb (2.3 ${mu}SI$). Based on the paleomagnetic analysis as well as modal analysis, the main susceptibilities of Gb and Ggb reside in magnetite and mafic minerals, respectively. They belong to S-type granite of non-magnetic granite by susceptibility value. In addition, $SiO_2$ contents, $K_2O/Na_2O$, A/CNK molar ratio and ACF diagram support that they all belong to S-type granites.

Study on Tree Growth and Soil Environment Relations of Sudokwon Landfill (수도권매립지수목생육과토양환경의관계연구)

  • Kang, Seong-Chil;Han, Bong-Ho;Choi, Jin-Woo;Jang, Jae-Hoon;Kim, Hong-Soon
    • Korean Journal of Environment and Ecology
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    • v.29 no.3
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    • pp.431-440
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    • 2015
  • This study investigated the correlation between the trees planted on the slope of the first landfill where the landfill was completed and the soil environment. 69 plots (($7{\sim}20m{\times}5{\sim}20m$) were planted with 26 kinds of major trees in the first landfill slopes for vegetation survey. The survey was conducted from 2007 to 2009. Soil analysis from 2007 to 2008 was made on the basis of the injured index and the indicator species were selected. Depending on the rate of growth of the indicator species, seven survey locations were selected for soil sample analysis. 10 tree species appeared to adapt to the landfill environment. Among them, the coniferous trees were Pinus thunbergii (Seedling) and Juniperus chinensis and the deciduous trees were Quercus aliena, Salix preudo-lasiogyne, Castanea crenata (Seedling), Prunus armeniaca var. ansu, Quercus acutissima, Chionanthus retusus, Liriodendron tulipifera (Seedling) and Celtis sinensis. 8 species of trees did not adapt well to the landfill environment. Among them, two kinds were coniferous, the other six kinds were deciduous. In addition, judgment on the other 8 species of trees as to whether they adapted well or not was postponed. The species for which judgment was postponed included one coniferous tree and seven deciduous trees. Indicator species of injured index was the species that have more than 25 % of the defect. The soil was collected from where Catalpa ovata, Sophora japonica, Albizzia julibrissin and Acer palmatum were planted for analysis. According to the soil analysis, the soil pH showed that the soil was alkaline. Regarding the three phases of the soil, most sample soil showed 60 % for the solid phase or 55 % for the solid phase but with less than 10 % of gas phase. The organic matter content was found to be 0.14 to 2.52 %.

Quality characteristics of white (Baktae) and black (Seoritae) soybean curds coagulated by Omija extract (오미자 추출액을 응고제로 이용한 백태 및 서리태 두부의 품질특성)

  • Cheigh, Chan-Ick;Moon, Hey-Kyung;Lee, Su-Won;Kim, Jong-Kuk
    • Food Science and Preservation
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    • v.23 no.1
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    • pp.42-48
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    • 2016
  • In order to investigate the utilization of the Omija (Schizandra chinensis Baillon) extract as a natural coagulant for manufacturing soybean curd, the quality characteristics of white (Baktae) and black (Seoritae) soybean curds, coagulated by the Omija extract or $MgCl_2$, were evaluated. Crude protein ($6.14{\pm}0.30$ and $6.25{\pm}0.18%$, respectively) and crude lipid ($10.86{\pm}1.74$ and $11.29{\pm}1.69%$, respectively) contents of white and black soybean curds coagulated using the Omija extract were higher than those coagulated using $MgCl_2$. Black soybean curds coagulated using the Omija extract showed higher L, a, and b values than those using $MgCl_2$. The most abundant amino acid in white and black soybean curds coagulated using the Omija extract was arginine (3.74 and 3.71 mg/100 g, dry basis, respectively). The amounts of Ca, K, Mg, and Na were the highest in both soybean curds prepared with the Omija extract. The sensory evaluation (color, flavor, taste, texture, and overall preference) showed that white and black soybean curds coagulated using the Omija extract were more preferred than those produced using $MgCl_2$. The results suggested that using the Omija extract as a natural coagulant agent could improve the quality and sensory characteristics of soybean curds.

Uptake of Heavy Metal Ions by Water Dropwort (Oenanthe stolonifera DC.) and Identification of Its Heavy Metal-Binding Protein (미나리의 중금속 흡수량 측정 및 중금속 결합단백질의 동정)

  • Park, Young-Il;Kim, Hee-Guen;Kim, Yoo-Young;Kim, In-Soo
    • Applied Biological Chemistry
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    • v.39 no.6
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    • pp.494-500
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    • 1996
  • Uptake of hen metal ions by water dropwort (Oenanthe stolonifera DC.) and its cadmium-binding protein were studied to probe for good method to remove heavy metal contaminants from environments. The plant was cultured in the culture medium (pH 7.0) containing the various concentrations of $Cd^{2+}$, $Cr^{3+}$ or $Pb^{2+}$, for 3 and 7 days. The residual heavy metals deposited in roots linearly increased as the metal ions concentration increased up to 17 ppm for $Cd^{2+}$, 20 ppm for $Cr^{3+}$ and 50 ppm for $Pb^{2+}$. Above these concentrations, the plant growth was inhibited and the uptake rates of the metal ions decreased. The heavy metals absorbed by the plant were mostly deposited in roots. In particular, the residual concentration of lead in roots was about four times higher than those of cadmium and chromium. When cultured in the medium containing 20 ppm of each metal ion, 80% of cadmium, 90% of cromium and 96% of lead were deposited in roots out of the total residual metal ions in the plant. These values correspond to 6.1 mg of cadmium, 5.2 mg of chromium and 23.6 mg of lead per one gram of roots tissue on a dry weight basis. A cadmium-binding protein was partially purified by extraction, gel filtration and DEAE-Cellulose chromatography from water dropworts that was grown in the medium containing 20 ppm $Cd^{2+}$. The purified protein was a single band on SDS- and non-denaturing- polyacrylamide gel electrophoresis. Its molecular mass was estimated to be ca. 5,000 dalton by gel filteration. Analysis of amino acid composition of the protein indicated that it had a typical amino acid composition of heavy metal-binding protein in that it contained 27% of acidic amino acids and 9.9% of cysteine. However, it is likely that the protein is a new plant metal-binding protein, since its amino acid composition is somewhat different from those of phytochelatins that have been known so far.

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Analysis on Nutrient Intakes and Eating Behaviors of Female Students Visiting Nutrition Counselling Office (체중조절 내담자의 영양섭취 및 식행동 실태 분석)

  • 이보숙;권순형;허채옥;조경련;이영순;김명자;임호남
    • Journal of the East Asian Society of Dietary Life
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    • v.12 no.5
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    • pp.352-363
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    • 2002
  • The purpose of this study was to analyze health-related habits, weight control experience and body composition of 344 female students visiting nutrition counselling office. Dietary habits of the subjects were assessed by means of interview with questionnaire and nutrient intakes were evaluated by the simple dietary assessment method. Weight and height were measured to get body mass index(BMI) and waist-hip ratio and their body composition were measured by Inbody 3.0. The obtained results were as follows: 1) Among students, 14.5% were exercising on a regularly basis, of which the exercises were walking and gymnastic exercise(22.1%), jogging(14.1%), and rope-skipping(4.7%), 48.4% of students were little drinking and 30.5% were once a week and smokers were 4.1%. 2) 28.3% of students were little having breakfast and 15.1% were once or twice a week. Two third of total students were not having breakfast regularly. Also only 59.6% of students were having dinner everyday, which means many of them were even skipping dinner. The reasons why they were not having breakfast were because they don't have enough time to eat(66.0%) and for a diet(2.8%) and the reasons for skipping dinner were because they were not hungry(23.0%), for the weight loss(18.2%). It was shown that they would skip dinner rather than breakfast for a weight control. 51.6% of students were taking snack 1~2 times a day and 5.3% were having little snack. 55.4% of students were dining out once a day and 15.4% more am 2 times a day. 3) 46.7% of students were already experiencing weight control before visiting the counselling office. 78.5% of students tried on one kind of weight control method, 11.4% on two kinds, and 10.1 % on more than three kinds. The method they tried for a weight control most was the one food diet using egg, fruits and beans, which is the most popular among them, and the next were an aerobic exercise(23.6%). a diet tried by a famous entertainer (15.5%), and the fasting(14.5%). 4) The average BMI was 21.2 and the body fat rate was 28.1%. As a result of grouping BMI, 12.8% were underweight 67.6% normal weight 11.6% overweight and 8% obesity. Less than standard for the body fat rate were 0.6%, 50.6% standard, 48.5% more than standard. 49.2% of students as normal weight on a BMI were assessed the so-called "skinny obesity. 50% of consulted students situated fat intra-abdominally at the umbilical level(WHR>0.8). 5) The energy intake was 76.6$\pm$17.8% of RDA and constituent ratio of carbohydrate, fat and protein were 64.2$\pm$5.2%, 21.6$\pm$3.7% and 14.3$\pm$2.3%, respectively, which is little over of 20% of recommended ratio of fat. Protein and niacin intake were more than 90% of RDA and riboflavin and vit. C were taken more than 100% of RDA. But Fe intake was 69.4$\pm$19.3%, Ca 76.6$\pm$23.6%, which were the least constituents. There was not remarkable significance between energy intake and nutrient density based on the groups of underweight, normal weight, overweight and obesity of BMI. 6) It was shown that body fat rate had remarkably significant correlation(p<0.000) with BMI(r=0.760) and WHR(r=0.817) respectively, but body fat rate was more correlated with WHR than with BMI. There was not much significant difference between body fat rate and WHR whether they exercised or not. However. BMI was significantly higher in the exercise group because one who showed higher BMI started to exercise since they looked fat in appearance and perceived as they were fat. fat.

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The Effects of Prostaglandin and Dibutyryl cAMP on Osteoblastic Cell Activity and Osteoclast Generation (Prostaglandin과 Dibutyryl cAMP가 조골세포의 활성과 파골세포 형성에 미치는 영향)

  • Mok, Sung-Kyu;You, Hyung-Keun;Shin, Hyung-Shik
    • Journal of Periodontal and Implant Science
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    • v.26 no.2
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    • pp.448-468
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    • 1996
  • To maintain its functional integrity, bone is continuously remodelled by a process involving resorption by osteoeclasts and formation by osteoblasts, In order to respond to changes in the physical environment or to trauma with the relevant action, this process is strictly regulated by locally synthesized or systemic fators, Prostaglandin $E_2(PGE_2$) is perhaps one of the best studied factors, having been known to affect bone cell function for several decades.$PGE_2$ has both anabolic and catabolic activities. Excess of $PGE_2$ has been implicated in a number of pathological states associated with bone loss in a number of chronic inflammatory conditions such as periodontal disease and rheumatoid arthritis. $PGE_2$ and other arachidonic acid metabolites have been shown to be potent stimulators of osteoclastic bone resorption in organ culture. The anabolic effects of $PGE_2$ were first noticed when an increase in periosteal woven bone formation was seen after the infusion of $PGE_2$ into infants in order to prevent closure of the ductus arteriosus. The cellular basis for the catabolic actions of $PGE_2$ has been well characterized. $PGE_2$increases osteoclast recruitment in bone marrow cell cultures. Also $PGE_2$ has a direct action on osteoclast serving to inhibit activity and can also indirectly activate osteoclast via other cells in the vicinity, presumably osteoblast. The cellular mechanisms for the anabolic actions of $PGE_2$ are not nearly so well understood. The purpose of this paper was to study the effects of $PGE_2$ and dibutyl(DB)cAMP on osteoblastic clone MC3T3El cells and on the generation of osteoclasts from their precursor cells. The effect of $PGE_2$ and DBcAMP on the induction of alkaline phoaphatase(AlP) was investigated in osteoblastic clone MC3T3El cells cultured in medium containing 0.4% fetal bovine serum. $PGE_2$ and DBcAMP stimulated ALP activity and MTT assay in the cells in a dose-dependent manner at concentrations of lO-SOOng/ml. Cycloheximide, protein synthesis inhibitor, inhibited the stimulative effect of $PGE_2$ and DBcAMP on ALP activity in the cells. $PGE_2$also increased the intracellular cAMP content in a dose-dependent fashion with a maximal effect at 500ng/ml. The effect of $PGE_2$ on the generation of osteoclasts was investigated in a coculture system of mouse bone marrow cells with primary osteoblastic cells cultured in media containing 10% fetal bovine serum.After cultures, staining for tartrate-resistant acid phosphatase(TRAP)-marker enzyme of osteoclast was performed. The TRAP(+) multinucleated cells(MNCs), which have 3 or more nuclei, were counted. More TRAP(+) MNCs were formed in coculture system than in control group. $PGE_2(10^{-5}10^{-6}M)$ stimulated the formation of osteoclast cells from mouse bone marrow cells in culture. $PGE_2(10^{-6}M)$ stimulated the formation of osteoclast cells from mouse bone marrow cells in coculture of osteoblastic clone MC3T3E1 cells This results suggest that $PGE_2$ stimulates the differentiation of osteoblasts and generation of osteoclast, and are involved in bone formation, as well as in bone resorption.

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Relation Among Parameters Determining the Severity of Bronchial Asthma (기관지천식 환자의 증상의 중증도를 나타내는 지표들간의 연관성)

  • Lee, Sook-Young;Kim, Seung-June;Kim, Seuk-Chan;Kwon, Soon-Suk;Kim, Young-Kyoon;Kim, Kwan-Hyoung;Moon, Hwa-Sik;Song, Jeong-Sup;Park, Sung-Hak
    • Tuberculosis and Respiratory Diseases
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    • v.49 no.5
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    • pp.585-593
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    • 2000
  • Background : International consensus guidelines have recently been developed to improve the assessment and management of asthma. One of the major recommendation of these guidelines is that asthma severity should be assessed through the recognition of key symptoms, such as nocturnal waking, medication requirements, and objective measurements of lung function. Differential classification of asthma severity would lead to major differences in both long term pharmacological management and the treatment of severe exacerbation. Methods : This study examined the relationship between the symptom score and measurements of $FEV_1$ and PEF when expressed as a percentage of predicted values in asthmatics (n=107). Results : The correlation of $FEV_1$ % with PEFR% was highly significant (r=0.83, p<0.01). However, there was agreement in terms of the classification of asthma severity in 76.6% of the paired measurements of $FEV_1$ % and PEFR%. Agreement in the classification of asthma severity was also found in 57.1% of the paired analysis of $FEV_1$ % and symptom score. 39% of the patients classified as having moderate asthma on the basis of $FEV_1$ % recording would be considered to have severe asthma if symptom score alone were used. Low baseline $FEV_1$ and high bronchial responsiveness were associated with a low degree of perception of airway obstruction. Conclusion : The relationships between the symptom score, PEFR and $FEV_1$ were generally poor. When assessing asthma severity, age, duration, $PC_{20}$, and baseline $FEV_1$ should be considered.

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Supercargo and Temporary Passengers (화물관리인과 임시승선자)

  • Choi, Suk-Yoon;Hong, Sung-Hwa;Ha, Chang-Woo
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2018.11a
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    • pp.225-227
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    • 2018
  • This research paper examines the history and present of 'temporary passengers' prescribed in Paragraph 9 of Article 5 of the Ships Safety Act Enforcement Regulations and suggests improvement plans referring to the examples of legislation of other countries. In 2015, Ministry of Ocean and Fisheries made authoritative interpretation that Paragraph 9 of Article 5 of the Ships Safety Act Enforcement Regulations, which prescribes special cargo drivers such as agricultural, marine or livestock vehicles as temporary passengers, is applied only to passenger ships and not to cargo ships such including Ro-Ro cargo ships. As the authoritative interpretation of the Ministry does not agree with not only the traditional interpretational methodology but also the interpretational methodology that are commonly used today, it lacks logical basis and looks unpersuasive. Paragraph 9 of Article 5 of the Ships Safety Act Enforcement Regulations can be applied not only on passenger ships but also on cargo ships. Also in case of Ro-Ro cargo ships, it is logically contradictory and against fairness not to acknowledge special cargo vehicle drivers as temporary passengers when there is no problem with safe navigation and safety of passengers on board even when the sailor, the sailor's family and the ship owner may be acknowledged as temporary passengers. To avoid unnecessary disputes and lawsuits, improvement plans using theory of legislation through statutory reform is more desirable. Therefore, the P aragraph should be amended to "Supercargo who deals with cargo that requires special care due to the characteristics of the cargo, such as transportation vehicles for agricultural products, marine products, livestock, explosives or flammable materials (drivers can serve as supercargos)" to reflect the distinct characteristics of cargo and ship navigation in Korea including the current distribution system, while setting an objective standard based on common sense of ordinary people and not on arbitrary interpretation.

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