• 제목/요약/키워드: ovulation detection

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개의 발정 진단 방법에 따른 효율 비교 (Comparison of Various Methods for Estrus Stage Determination in Bitch)

  • 고영진;강은주;이성림
    • 한국수정란이식학회지
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    • 제24권3호
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    • pp.131-137
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    • 2009
  • In dogs, correct diagnosis of estrus is important and the exact time of ovulation can be determined by variouse methods. Vaginal cytology has commonly used in conjunction with the physical examination, clinical history, vaginoscopy, and hormonal assays to determine the stage of the reproductive cycle. This study was therefore investigated the effectiveness of direct ovulation detector designed by changes of electrical resistance in vaginal mucus following different estrus cycles with several methods; vaginal cytology, concentration of plasma estrogen and progesterone, and direct examination by laparotomy. A total of 12 bitches was selected for the study and observed estrus signs. The bitches were evaluated clinical sign (vulvar swelling and bleeding), cytological examination (keratocyte and RBC), electrical resistance, plasma estrogen and progesterone concentration for estrus assessment. Accuracy of ovulation detection by vaginal cytology was significantly (p<0.05) lower than those by electrical resistance and plasma progesterone concentration, based on the confirmation by laparotomy. Vaginal smear is not confidential method compared to detection of electrical resistance and plasma progesterone concentration at ovulation. Although the value of electrical resistance was varied at the same points of estrus in individuals, ovulation was occurred at the first day which shown the peak of electrical resistance and mating time was third day after peak. In conclusion, ovulation detector designed by changes of electrical resistance is an effective and economic instrument for predicting estrus and ovulation in bitches.

Ovulation Synchronization in Water Buffaloes Guided by Milk Progesterone ELISA

  • Hoque, Md. Nazmul;Talukder, Anup Kumar;Kamal, Md. Mostofa;Jha, Ajit Kumar;Bari, Farida Yeasmin;Shamsuddin, Mohammed
    • 한국수정란이식학회지
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    • 제26권2호
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    • pp.105-109
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    • 2011
  • Ovulation synchronization (ovsynch) has proved to increase the number of insemination in cattle by overcoming the problems of heat detection. The aim of this study was to do ovsynch in water buffaloes where heat detection is a major reproductive problem and to determine the conception rates after timed artificial insemination (TAI). Twenty cyclic buffaloes at ${\geq}$ 60 days postpartum were selected by examining 24 unobserved estrus buffaloes based on milk progesterone assay (progesterone concentration ${\geq}$ 1.0 ng/ml) from the Mymensingh district of Bangladesh. Ovsynch treatment regimen was started irrespective of the stage of estrous cycle. Gonadorelin (500 ${\mu}g$) was injected intramuscularly at Day 0 followed by Alfaprostol (8 mg) at Day 7. A second injection of Gonadorelin was given at Day 9 and TAI was done with frozen semen from Mediterranean buffalo bulls at 16~20 hours of the second Gonadorelin injection. Milk progesterone ELISA at Day 10~12 post AI confirmed ovulation in 16 out of 20 (80%) buffaloes (progesterone concentration ${\geq}$ 1.0 ng/ml). High progesterone concentration (${\geq}$ 1.0 ng/ml) at Day 10~12 and Day 22~24 of AI showed pregnancy in six out of 20 (30%) buffaloes. Pregnancy was further confirmed by ultrasonography at Day 40 in these six buffaloes. In conclusion, ovsynch followed by TAI could be applied in cyclic buffaloes for overcoming the estrus detection problems; however, more studies are needed to increase the conception rate.

Application of Ultrasonography to Investigate Postpartum Anestrus in Water Buffaloes

  • Rahman, Md Saidur;Shohag, Abu Said;Kamal, Md. Mostafa;Parveen, Nasreen;Shamsuddin, Mohammed
    • Reproductive and Developmental Biology
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    • 제36권2호
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    • pp.103-108
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    • 2012
  • Anestrus is one of the most important production limiting disorders in dairy buffaloes and its underlying causes have been a current topic of studies. The objectives of this study were to explore the causes of anestrus in buffaloes with the application of ultrasonography. Two examinations were performed by transrectal ultrasonography at 12 days apart in buffalo cows that were not seen in oestrus at 60 or more days postpartum. As high as 54.5% buffaloes had silent ovulation and 45.5% suffered from the true anestrus with ovarian dysfunction. The duration of anestrus after calving was 60~90, 91~120, 121~180 and 181~365 days in 27%, 32%, 18% and 23% buffalo cows, respectively. Treatment with prostaglandin of cyclic buffalo cows with a corpus luteum (72.7%) resulted in higher estrous rate as compared with close observation of estrus (23.1%) by the farmer (p=0.021). Acyclic buffalo cows without any corpus luteum on ovaries were successfully treated with gonadotropin releasing hormone (70%), resulting in higher estrous detection rate than those treated with a vitamin-mineral mixture (20%) (p=0.035). In conclusion, poor heat detection due to silent ovulation is the most important cause of apparent anoestrus in buffaloes; however the percentage of the true anestrous is also quite high in postpartum buffaloes.

Shih-tzu견에서 임신일령에 따른 임신구조물의 초음파상 I. 태아 및 태아외구조물의 최초 관찰시기 (Ultrasonographic Appearance of the Gestational Structures Throughout Pregnancy in Shih-tzu bitches I. Time of Initial Detection of the Fetal and Extra-fetal Structures)

  • 고진성;김방실;이순애;조양택;김재풍;오기석;김성호;김종택;박인철
    • 한국임상수의학회지
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    • 제21권1호
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    • pp.29-34
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    • 2004
  • Serial ultrasonographic examinations were daily performed on 12 Shih-tzu bitches from 15 days after ovulation until parturition to determine the time of first detection and ultrasonographic appearance of the fetal and extra-fetal structures and to determine the size of gestational structures. Gestational age was timed from the day of ovulation (day 0), which was estimated to occur when plasma progesterone concentration was first increased above 4.0 ng/ml. The gestational length in 12 Shih-tzu bitches was 62.1 (range:60-64) days. The initial detection of the fetal and extra-fetal structures were: gestational sac at days 19.2 (16-21); zonary placenta in the uterine wall at days 25.9 (24-28); yolk sac membrane at days 24.1 (22-26); amniotic membrane at days 26.6 (24-28); embryo initial detection at days 22.3 (21-24); heartbeat at days 23.8 (23-25); limb buds at days 29.3 (27-31); fetal movement at days 32.3 (31-34); stomach at days 32.6 (29-34); urinary bladder at days 33.1 (29-35); skeleton at days 35.9 (34-38) and kidney at days 45.8 (45-48).

젖소의 다양한 발정 행동 징후와 배란 시간과의 관계 (Relationship between Various Estrous Behavioral Signs and Ovulation Time in Dairy Cows)

  • 손준규;박수봉;박성재;백광수;안병석;김현섭;황석주;주종철;박춘근
    • 한국수정란이식학회지
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    • 제22권1호
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    • pp.9-13
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    • 2007
  • 본 연구는 젖소의 발정 행동 징후에 따라 배란 시간을 예측하고 결정하기 위하여 발정의 다양한 행동 징후(승가, 승가허용, 외음부 냄새 맡기 및 턱비빔)와 배란 시간과의 관계에 대해 조사하였다 공시된 51두 중 37두에서 배란이 일어났으며, 이 중 28두에서 발정 발현이 나타났다. 발정 행동 징후는 $PGF_2{\alpha}$주사 후 2일째부터 72시간 동안 육안 관찰하였으며 배란 시간(4시간 간격으로 초음파 검사)과의 관계를 각각 조사하였다. 발정 행동별 발정 발현율은 외음부 냄새와 턱비빔은 81%와 78%를 보였으며, 승가는 78% 승가 허용은 56%를 보였다. 발정 행동 징후별 발정 개시와 종료의 배란 시간까지의 간격 분포는 발정 개시 후 배란까지는 배란 전 $7{\sim}37$시간(평균 $25.58{\pm}7.94$ 시간)사이였으며, 발정 종료 후 배란까지는 배란 전 $1{\sim}28$시간(평균 $13.42{\pm}7.14$ 시간) 사이였다. 발정 행동별 발정 개시 후 배란까지의 시간에서 승가 허용($17.33{\pm}5.83$시간)이 승가($23.58{\pm}5.12$ 시간), 외음부 냄새 맡기$(24.25{\pm}6.09$시간), 턱비빔($23.42{\pm}6.04$ 시간)보다 유의적으로(p<0.05) 짧게 나타났다. 또한 발정 행동별 배란 전 발정 개시에서 승가, 승가 허용, 외음부 냄새 맡기 및 턱비빔은 12시간 간격 안에서 $16{\sim}28$시간(88%), $10{\sim}22$시간(81%), $16{\sim}28$시간(79%) 및 $19{\sim}31$시간(79%)사이에서 각각 개시하였다. 외음부 냄새 맡기와 턱비빔 행동은 발정 단계가 아닐 때도 보이는 행동이기 때문에 배란 시간을 예측하기에는 적절하지 않다. 결론적으로, 승가 허용와 승가 행위는 배란 시간을 예측하는데 좋은 지표이지만, 승가 허용은 승가를 허용하는 개체의 수가 제한적이기 때문에 배란 시간을 예측하는데 다소 어려운 실정이다. 따라서 승가 행위로 배란 시간을 예측하는 것이 훌륭한 지표가 될 것으로 생각된다.

소와 돼지에서 발정관찰과 이상발정의 대책 (Observation of Estrus and Control of Abnormal Estrus in Cattle and Pig)

  • 김창근
    • 한국가축번식학회지
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    • 제7권2호
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    • pp.72-85
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    • 1983
  • This presentation firstly is discussed the characteristics of estrus, the time of first postpartum estrus, and the relative accurate of various estrus detection aids and secondly discussed the abnormalities of estrus and ovarian function and its control by treatment of exogenous hormones in cattle and pig. Longer estrus cycles as well as the shorter than 18 day cycles showed the lowered conception rates as compared to the normal cycles of 18 to 25 days. Other characteristics of est겨s such as duration of estrus, intensity of estrus and time of estrus are reviewed to affect fertility. The first postpartum ovulation and estrus in cows usually occurs about 20 to 30 days and 40 to 50 days after parturition, respectively. Irregularities in estrus cycle length have been conducted during early postpartum period. In sows, weaning is followed by ovulation and estrus although there is some individual variation. The most common method of estrus detection is direct visual observation on standing estrus behavior, but various aids of estrus detection have been empolyed with varying degree of effectiveness. The results from heat detector devices are about as accurate as twice-daily observation(about 90%). The abnormal estrus can be classified into three types; irregular or continuous estrus, silent estrus and anestrus. Cystic ovarian disease, follicular cysts and luteal cysts, is a serious cause of reproductive failure in cattle and pig. The follicular cysts are much more common than luteal cysts and the incidence of ovarian cysts in dairy cattle is higher than beef cattle and pig. The occurrences of ovarian cysts have been closely associated with levels of milk production, stages of postpartum period, nutritional levels and seasons. The luteal cysts and persistent corpora lutea are responsive to the luteolytic effects of the recently synthetic analogues of PGF2$\alpha$ in cows and sows and recently GnRH or LH-RH has been successfully used as a treatment for cows and sows with ovarian follicular cysts.

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The use of infrared thermography to detect the stages of estrus cycle and ovulation time in anatolian shepherd dogs

  • Olgac, Kemal Tuna;Akcay, Ergun;Cil, Beste;Ucar, Burak Mehmet;Daskin, Ali
    • Journal of Animal Science and Technology
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    • 제59권10호
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    • pp.21.1-21.6
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    • 2017
  • Background: The aim of the study is to evaluate the effectiveness of thermographic monitoring, using the temperature changes of perianal and perivulvar areas for the determination of estrus in Anatolian Shepherd bitches. Fifteen bitches were used in the study. Blood and vaginal smear samples were collected and thermographic monitoring of perianal and perivulvar areas were carried out starting from proestrus to early diestrus. Also, external signs of estrus were investigated. Smear samples were evaluated by light microscopy after Diff-Quik staining method and superficial and keratinized superficial cells were determined as percentage (S + KS%). Progesterone and luteinizing hormone measurements were done by radioimmunoassay. The difference in temperature between perianal and perivulvar areas was evaluated through thermographic images by FLIR ResearchIR Software. Results: According to the results obtained from the study, differences between progesterone and S + KS% were statistically significant (P < 0,05). Although temperature showed increase and decrease with progesterone and S + KS%, the differences were not important statistically (P > 0,05). Serum luteinizing hormone levels did not sign any difference (P > 0,05). Conclusions: As a result, thermographic monitoring alone is not enough for estrus detection in Anatolian Shepherd bitches. However, it can be used to assist the actual estrus detection technique in terms of providing some foreknowledge by evaluating the differences in temperature.

소형 애완견에서 초음파 검사에 의한 임신 구조물의 최초 관찰 시기 (Time of Initial Detection of the Gestational Structures by Ultrasonography Examination in Small Pet Dogs)

  • 박상국;김방실;윤창진;여운창;박철호;김재풍;이숙경;문진산;서국현;오기석;손창호
    • 한국수정란이식학회지
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    • 제23권1호
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    • pp.5-11
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    • 2008
  • Serial ultrasonographic examinations were daily performed from 15 days after ovulation until parturition to determine the time of first detection and ultrasonographic appearance of the fetal and extra-fetal structures in pregnant 10 Maltese, 10 Yorkshire Terrier, 15 Shih-tzu, and 10 Miniature Schnauzer bitches, respectively. Gestational age was timed from the day of ovulation (day 0), which was estimated to occur when plasma progesterone concentration was first increased above 4.0ng/ml. The gestational length was $63.4{\sim}63.6$ (range: $61{\sim}65$) days and the geatational length was no statistically significant difference among bitches (p>0.05). The initial detection of the extra-fetal structures were; gestational sac at days $18.9{\sim}19.5\;(17{\sim}22)$, zonary placenta at days $24.6{\sim}25.5\;(23{\sim}28)$, yolk sac membrane at days $24.6{\sim}25.5\;(23{\sim}27)$, yolk sac tubular shape at days $26.1{\sim}26.3\;(24{\sim}28)$, and amniotic membrane at days $26.1{\sim}28.2\;(24{\sim}31)$, respectively. The time of the first detection of the extra-fetal structures were no statistically significant difference among bitches (p>0.05). The initial detection of the fetal structures were; embryo initial detection at days $22.5{\sim}22.9\;(21{\sim}24)$, heartbeat at days $23.2{\sim}23.8\;(21{\sim}25)$, embryo bipolar shape $27.6{\sim}28.9\;(26{\sim}30)$, fetal movement at days $31.9{\sim}32.8\;(27{\sim}34)$, limb buds at days $29.1{\sim}30.7\;(27{\sim}33)$, stomach at days $31.1{\sim}33.1\;(29{\sim}34)$, urinary bladder at days $32.4{\sim}33.2\;(29{\sim}35)$, skeleton at days $34.7{\sim}35.9\;(34{\sim}39)$, and kidney at days $42.1{\sim}44.7\;(41{\sim}48)$, respectively. The the time of the first detection of the fetal structures were no statistically significant difference among bitches (p>0.05). These results indicate the evaluation of the time of first detection and ultrasonographic characteristics of the gestational structures might be useful for pregnancy diagnosis, estimating fetal age, embryonic resorption, fetal monster, abnormal fetal growth and fetal viability, respectively.

배란유도주기에 따른 초음파검사와 기초체온표의 비교분석 (A Comparative Analysis of Basal Body Temperature to Ultrasound, as a Method of Ovulation Detection in Induced Ovulatory Menstrual Cycles)

  • 최욱;서병희;이재현
    • Clinical and Experimental Reproductive Medicine
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    • 제12권2호
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    • pp.25-37
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    • 1985
  • Four points on the basal body temperature (B.B.T.) curve was correlated with the estimated time of ovulation, as determined by serial ultrasound in 50 induced menstrual cycles from 22 subjects. The time of ovulation was estimated by measuring the maximal diameter of follicles and observing the morphologic changes within the ovary from follicle to corpus luteum. The results were as following; 1. The diameter of the follicle measured at the day before disappearance was 21.1 mm on an average (S.D.: 2.14). The average follicular growth for 4 days before ovulation was measured at a rate of 2.8 mm/day, and rapid growth of follicle was observed 3.1 mm/day at the day before. 2. The changes associated with rupture of the follicles were the followings, in order of frequency; decrease in size(94%), disappearance of follicles(64%), fluid in the Cul-de-Sac(26%) and increased internal echoes(16%). 3. Only 20 of 50 cycles, exhibited a BBT dip and correlated with the estimated time of ovulation by ultrasound in 2 of which cases(10%). BBT nadir, 30 of 50 cycles, correlated in 5(16.7%). The first day of hyperthermic plateau(FDHP) and BBT coverline was exhibited in all cycles, correlated in 41(82%) and 35(70%) cases. 4. The relationship between the diameter of dominant dominant follicle, measured by ultrasound, and the basal body temperature curve were as following. During cycles in which dip was observed on the BBT curve, the follicular diameter were 10.5${\pm}$2.12 mm on 4 days prior to the point (D-4), and 12.5${\pm}$2.12 mm (D-3), 15.5${\pm$2.12 mm (D-2), 17.0${\pm}$1.41 mm (D-1) and 21.5${\pm}$2.12 mm just prior to the dip (D-0). In the nadir; 9.6${\pm}$1.67 mm (N-4), 12.8${\pm}$1.79 mm (N-3), 16.2${\pm}$1.92 mm (N-2), 18.2${\pm}$2.17 mm (N-1) and 21.4${\pm}$2.61 mm (N-0). In the First day of Hyperthemic Plateau (FDHP); 9.8${\pm}$1.36 mm (F-4), 12.4${\pm}$1.41 mm (F-3),15.1${\pm}$1.57 mm (F-2), 18.1${\pm}$1.67 mm (F-1) and 21.2${\pm}$2.25 mm (F-0). In the BBT coverline endopint; 9.9${\pm}$.39 mm (C-4), 12.5 ${\pm}$1.44 mm (C-3), 15.2${\pm}$1.64 mm (C-2), 18.0 ${\pm}$1.69 mm (C-1), and 21.2${\pm}$2.31 mm (C-0). 5. The relationship between the ultrasonographic signs of ovulation and the basal body temperature curve were as following. The BBT dip correlated with the ovulation in 2 cases, which revealed decrease in follicular diameter (100%), fluid pattem in the Cul-de-Sac (1 case, 50%) and complete disappearance of follicle (1 case, 50%). In the nadir (5 cases); the ultrasonographic signs of ovulation were decrease in follicular diameter (5 cases, 100%), fluid pattern in the Cul-de-Sac (1 case, 20%) and complete disappearance of follicle (3 cases, 60%). In the First day of Hyperthermic Plateau (41 cases); decrease in follicular diameter (40 cases, 97.6%), fluid pattern in the Cul-de-Sac (11 cases, 26.8%), appearance of internal echo and thickening of the wall (6 cases, 14.6%) and com plete disappearance of follicle (28 cases, 68.3%). In the BBT coverline endpoint (35 cases); decrease in follicular diameter (33 cases, 94.3%), fluid pattern in the Cul-de Sac (9 cases, 25.7%), appearance of internal echo and thickening of the wall (5 cases 14.3%) and complete disappearance of follicle (20 cases, 57.1%).

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젖소 경산우와 미경산우의 발정 행동과 배란 시간의 비교 (Comparison of Estrous Behavior and Ovulation Time in Dairy Cows and Heifers)

  • 손준규;박수봉;박성재;백광수;이명식;안병석;김현섭;박춘근
    • 한국수정란이식학회지
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    • 제22권3호
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    • pp.185-190
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    • 2007
  • 본 연구는 젖소 경산우와 미경산우의 다양한 발정 행동 특성과 배란 시간을 조사하기 위해 수행되었다. 공시된 89두 중 73두에서 배란이 일어났으며, 이 중 61두에서 발정 발현이 나타났다. 다양한 발정 행동은 $PGF_2{\alpha}$ 투여 후 2일째부터 72시간 동안 육안 관찰하였으며, 배란 시간(4시간 간격으로 초음파 시험)과의 관계를 각각 조사하였다. 경산우의 발정 발현율은 외음부 냄새 맡기, 턱 비빔, 승가 및 승가 허용에서 각각 81%, 78%, 78% 및 56%로 나타났으며, 미경산우에서는 외음부 냄새 맡기, 턱 비빔, 승가 및 승가 허용은 각각 61%, 68%, 82% 및 76%를 보였다. 젖소 경산우와 미경산우에서 발정 개시 후 배란까지의 평균 $25.58{\pm}7.94$$25.55{\pm}5.72$시간이었으며, 발정 종료 후 배란까지는 평균 $13.42{\pm}7.14$$7.48{\pm}7.4$시간이었다. 경산우의 발정 개시 후 배란까지의 시간에서는 승가 허용($17.33{\pm}5.83$시간)이 승가, 외음부 냄새 맡기, 턱 비빔($23.58{\pm}5.12$시간, $24.25{\pm}6.09$시간, $23.42{\pm}6.04$시간)보다 유의적으로(p<0.05) 짧게 나타났으며, 미경산우에서는 유의적인 차이가 인정되지 않았다. 승가 허용 종료에서 배란까지의 시간에서는 미경산우($6.38{\pm}4.80$)가 경산우($13.05{\pm}4.53$)에 비해 유의적으로(p<0.05) 짧게 나타났다. 본 연구 결과에 의해 우유생산에 관련한 생리적인 변화가 젖소 발정행동과 배란 특성의 변화의 주요 요인일 수 있다고 사료된다.