• IMMUNE NETWORK
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• 제9권5호
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• pp.179-191
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• 2009

Background: The present study examines a hypothesis that short allergen-derived peptides may shift an Aspergillus fumigatus (Afu-) specific TH2 response towards a protective TH1. Five overlapping peptides (P1-P5) derived from Asp f1, a major allergen/antigen of Afu, were evaluated for prophylactic or therapeutic efficacy in BALB/c mice. Methods: To evaluate the prophylactic efficacy, peptides were intranasally administered to naive mice and challenged with Afu-allergens/antigens. For evaluation of therapeutic efficacy, the mice were sensitized with Afu-allergens/antigens followed by intranasal administration of peptides. The groups were compared for the levels of Afu-specific antibodies in sera and splenic cytokines evaluated by ELISA. Eosinophil peroxidase activity was examined in the lung cell suspensions and lung inflammation was assessed by histopathogy. Results: Peptides P1-, P2- and P3 decreased Afu-specific IgE (84.5~98.9%) and IgG antibodies (45.7~71.6%) in comparison with Afu-sensitized mice prophylactically. P1- and P2-treated ABPA mice showed decline in Afu-specific IgE (76.4~88%) and IgG antibodies (15~54%). Increased IgG2a/IgG1 and IFN-${\gamma}$/IL-4 ratios were observed. P1-P3 prophylactically and P1 therapeutically decreased IL-5 levels and eosinophil peroxidase activity. P1 decreased inflammatory cells' infiltration in lung tissue comparable to non-challenged control. Conclusion: Asp f1-derived peptide P1, prophylactically and therapeutically administered to Balb/c mice, is effective in regulating allergic response to allergens/antigens of Afu, and may be explored for immunotherapy of allergic aspergillosis in humans.

• 한국가축번식학회지
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• 제27권4호
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• pp.299-307
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• 2003

The effects of additions/deletions in glycosylated residues of recombinant human EPO (rhEPO) produced in CHO-K1 on their secretion were examined. hEPO cDNA was amplified from human liver mRNA and cloned into the pCR2.1 TOPO. Using overlapping-extension site-directed mutagenesis method, glycosylation sites at 24th, 38th, 83rd, and 126th were respectively or accumulatively removed by substituting its asparagine (or serine) with glutamine. To add novel glycosylation sites, 69 and 105th leucine was mutated to asparagine. Mutant and wild type rhEPO constructs were cloned into the pcDNA3 expression vector with CMV promoter and transfected into CHO cell line, CHO-K1, to produce mutant rhEPO mutant rhEPO proteins. Enzyme-linked immunosorbant assay (ELISA) and Western analysis with monoclonal anti-EPO antibody were performed using supernatants of the cultures showing transient and stable expressions respectively. Addition of novel glycosylation reduced rhEPO secretion dramatically while deletion mutants had little effect except some double deletion mutants ($\Delta$24/83 and $\Delta$38/83) and triple mutant ($\Delta$24/38/83). This fact suggests that not single but combination of changes in glycosyl groups affect secretion of rhEPO in cell culture, possibly via changes in their conformations.

• 전자공학회논문지
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• 제51권4호
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• pp.69-75
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• 2014

본 논문에서는 하나의 서브블럭내에서 픽셀값정렬과 예측오차확장기법을 확장한 새로운 가역 정보은닉기법을 제안한다. 각 $2{\times}2$ 서브블록에서 최소값 그룹과 최대값 그룹으로 나누고, 최소값 그룹에서는 제일 작은 값에 비밀자료를 숨기고, 최대값 그룹에서는 제일 큰 값에 비밀자료를 숨긴다. 제안한 알고리즘은 평균 13,900 비트의 삽입용량을 가지는데 이 결과는 31.39dB의 시각적인 왜곡을 유지하면서 기존 알고리즘보다 4,553 비트 더 많이 숨길 수 있음을 실험을 통해서 보여주고 있다.

• 가족자원경영과 정책
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• 제18권1호
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• pp.141-162
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• 2014

The purpose of this research is to suggest some measures to improve policy issues by analyzing policy related to a child care service program in the community. Gilbert and Terrell's social policy analysis framework is used to analyze an after-school care service program for elementary school children. Furthermore, the Elementary Care Class(including the After-school Program), Youth After-school Academy, and Community Child Center, referred to as public care services, are employed to analyze community care service programs for children. The Elementary Care Class, Youth After-school Academy and Community Child Center are very similar in terms of the contents and application of the care service program for children, and mainly serve children in low-income groups. In addition, although user overlapping is an inevitable problem because the operating time of the Elementary Care Class and After-school Program coincides with that of the Youth After-school Academy and Community Child Center, it is structurally very hard to adjust the content of service, operating time, and so on because of differences in the delivery system for each program. Therefore, it is necessary to generate a unified delivery system to manage after-school care service programs for children and create a new special control tower to solve these problems. Furthermore, it is needed to extend the services to children from general double-income family, which is a blind spot of the care service.

• Journal of Plant Biotechnology
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• 제33권4호
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• pp.277-281
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• 2006

Fourier transform - infrared spectroscopy (FT-IR) provides biochemical profiles containing overlapping signals from a majority of the compounds that are present when whole cell extracts are analyzed. We attempted to determine the ripeness of strawberry fruit flesh by FT-IR. Fruit ripeness was divided into four developmental stages based on fruit skin color: 'yellow-green', 'pink-green', 'pink', and 'red' stages. Principal component analysis of FT-IR data of inside fruit flesh extracts clustered samples of four different developmental stages into three discrete groups: (1) 'yellow-green' group, (2) 'pink-green' group, and (3) 'pink' and 'red' group. The most remarkable difference between four different developmental stages was found in the carbohydrate fingerprint region $(1,000-1,100cm^{-1})$ of the FT-IR spectrum, indicating that differences in carbohydrate compounds represented the ripeness of strawberry fruit. Overall results indicate that FT-IR in combination with PCA enables discrimination of the ripeness of strawberry fruit flesh.

• IMMUNE NETWORK
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• 제14권3호
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• pp.138-148
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• 2014

MicroRNAs (miRNAs) are endogenous small RNA molecules best known for their function in post-transcriptional gene regulation. Immunologically, miRNA regulates the differentiation and function of immune cells and its malfunction contributes to the development of various autoimmune diseases including systemic lupus erythematosus (SLE). Over the last decade, accumulating researches provide evidence for the connection between dysregulated miRNA network and autoimmunity. Interruption of miRNA biogenesis machinery contributes to the abnormal T and B cell development and particularly a reduced suppressive function of regulatory T cells, leading to systemic autoimmune diseases. Additionally, multiple factors under autoimmune conditions interfere with miRNA generation via key miRNA processing enzymes, thus further skewing the miRNA expression profile. Indeed, several independent miRNA profiling studies reported significant differences between SLE patients and healthy controls. Despite the lack of a consistent expression pattern on individual dysregulated miRNAs in SLE among these studies, the aberrant expression of distinct groups of miRNAs causes overlapping functional outcomes including perturbed type I interferon signalling cascade, DNA hypomethylation and hyperactivation of T and B cells. The impact of specific miRNA-mediated regulation on function of major immune cells in lupus is also discussed. Although research on the clinical application of miRNAs is still immature, through an integrated approach with advances in next generation sequencing, novel tools in bioinformatics database analysis and new in vitro and in vivo models for functional evaluation, the diagnostic and therapeutic potentials of miRNAs may bring to fruition in the future.

• 복식문화연구
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• 제27권4호
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• pp.398-413
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• 2019

This study empirically analyzed the influence of fashion brands' marketing issues on actual sales and consumer preference-focusing on evaluation trends of brands over time by using the theoretical background and big data provided through literature. This study examined the influence of three fashion brands (Balenciaga, Vetements, and Off-White) that have recently seen a drastic increase in the number of searched volumes through social networks. To identify the consumer-brand evaluations and trends and the marketing issues, the time period was divided into Groups A and B, which are from 2014 to 2015 and from 2016 to 2017, respectively. This study analyzed the frequency of overlapping keywords by using the R program to graphically visualize the changes over the timeline. Specifically, this analysis extracted data mainly related to bags, wallets and accessories for 2014-2015, but in 2016-2017, all four brands saw a vast increase in the frequency of searching product keywords related to clothing and footwear, and newly extracted ones were the top keywords. When analyzing the big data with these keywords as indicators, I confirmed that the products related to bags, wallets, and accessories were shifted to those related to apparel and footwear. Consumers previously recognized luxury brands such as Balenciaga as accessories-oriented brands that were focused on handbags and sunglasses, but now they are gaining popularity and recognition among consumers as a fashion brand.

• Bulletin of the Korean Chemical Society
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• 제22권2호
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• pp.213-218
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• 2001

Presented are the STM images of self-assembled monolayer of 2-mercaptoethanol on Au(111). Striped structures of ($6{\times}3_{\frac{1}{2}}$), ($5{\times}3_{\frac{1}{2}}$), ($4{\times}3_{\frac{1}{2}}$) and compact-($5{\times}3_{\frac{1}{2}}$) were observed after annealing at $80^{\circ}C.$ Analysis of the ordered structures revealed that the basic fundamental units of the ordered structures were three crystallographically non-equivalent ($3_{\frac{1}{2}}{\times}3_{\frac{1}{2}}$) $R30^{\circ}$ assemblies, and that the way of combination of the assemblies produced the four different structures. The($6{\times}3_{\frac{1}{2}}$) structure ( $\theta$ = 0.33) was composed of one ($3_{\frac{1}{2}}{\times}3_{\frac{1}{2}}$)$R30^{\circ}$ assembly, while the ($5{\times}3_{\frac{1}{2}}$) ( $\theta$ = 0.30) and ($4{\times}3_{\frac{1}{2}}$) ( $\theta$ = 0.38) structures were consisted of two ($3_{\frac{1}{2}}{\times}3_{\frac{1}{2}}$) $R30^{\circ}$ assemblies, separated by 5a and 4a, respectively. Furthermore, the compact-(5X 3½) structure ( $\theta$ = 0.50) was obtained by overlapping three ($3_{\frac{1}{2}}{\times}3_{\frac{1}{2}}$) $R30^{\circ}$ assemblies. In spite of the diversity in the adsorption structures, all the adsorption sites of 2-mercaptoethanol were fundamentally identical. On the other hand, the unannealed primitive SAM of 2-mercaptoethanol was characterized by two observations: a short-range order keeping the adsorbed molecules at approximately $3_{\frac{1}{2}}$ a and the small domains of the striped structures supporting that the observed surface structures on the annealed surface were the extension of the primitive layer of 2-mercaptoethanol. Comparing these observations with the already published structures of ethanthiol, it was concluded that the interaction between the hydroxyl groups of 2-mercaptoethanol might play a significant role in the adsorption step of 2-mercaptoethanol on Au(111) to organize the adsorption structures different from those of ethanthiol.

• 산업융합연구
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• 제18권4호
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• pp.51-59
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• 2020

본 연구는 만성 뇌졸중 환자를 대상으로 저항운동이 포함된 거울치료 시 tDCS(transcranial direct current stimulation)의 융합 자극을 통해 마비측 다리의 근력과 보행 능력의 개선을 알아보고자 하였다. 12명의 뇌졸중 환자는 실험군 당 6명씩 무작위로 할당하였다. 실험군I(n=6)은 NDT(neurodevelopmental treatment)와 거울치료 시 sham tDCS의 융합자극군, 실험군II는 NDT와 거울치료 시 tDCS의 융합자극군으로 나누어 각각 30분씩, 주5회, 총4주 동안 실험을 실시하였다. 모든 평가는 중재 전과 중재 4주 후에 실시하였다. 다리근력은 측정한 모든 근육에서 실험군I에 비해 실험군II에서 유의한 차이를 나타냈고(p<.05), 변화량을 통한 군 간 비교에서는 quadriceps(p<.01)와 tibialis anterior(p<.01)에서 유의한 차이를 나타냈다. 보행능력은 실험군I과 실험군II 모두에서 유의한 차이를 나타냈고(p<.05), 변화량을 통한 군 간 비교에서 유의한 차이를 나타냈다(p<.01). 거울치료와 tDCS의 융합자극은 마비측 다리 근력 및 보행능력 향상에 중첩효과를 통한 긍정적인 개선 효과를 주는 것을 알 수 있었다.

• 천문학회보
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• 제43권1호
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• pp.41.1-41.1
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• 2018

We introduce a current status and future plans of Korea Microlensing Telescope Network (KMTNet) microlensing experiments, which include an observational strategy, pipeline, event-finder, and collaborations with Spitzer. The KMTNet experiments were initiated in 2015. From 2016, KMTNet observes 27 fields including 6 main fields and 21 subfields. In 2017, we have finished the DIA photometry for all 2016 and 2017 data. Thus, it is possible to do a real-time DIA photometry from 2018. The DIA photometric data is used for finding events from the KMTNet event-finder. The KMTNet event-finder has been improved relative to the previous version, which already found 857 events in 4 main fields of 2015. We have applied the improved version to all 2016 data. As a result, we find that 2597 events are found, and out of them, 265 are found in KMTNet-K2C9 overlapping fields. For increasing the detection efficiency of event-finder, we are working on filtering false events out by machine-learning method. In 2018, we plan to measure event detection efficiency of KMTNet by injecting fake events into the pipeline near the image level. Thanks to high-cadence observations, KMTNet found fruitful interesting events including exoplanets and brown dwarfs, which were not found by other groups. Masses of such exoplanets and brown dwarfs are measured from collaborations with Spitzer and other groups. Especially, KMTNet has been closely cooperating with Spitzer from 2015. Thus, KMTNet observes Spitzer fields. As a result, we could measure the microlens parallaxes for many events. Also, the automated KMTNet PySIS pipeline was developed before the 2017 Spitzer season and it played a very important role in selecting the Spitzer target. For the 2018 Spitzer season, we will improve the PySIS pipeline to obtain better photometric results.