• Title/Summary/Keyword: over-potential

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Protein Expression Profiling of Infected Murine Macrophage Cells (RAW 264.7) by Bacillus anthracis Spores

  • Seo Gwi-Moon;Nam Jeong-Ah;Oh Kwang-Gun;Chai Young-Gyu
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2003.05a
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    • pp.77-79
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    • 2003
  • Current therapeutic strategies far anthrax have had no significant impact on anthrax mortality over the last several decades. This study used a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) discovery platform to generate protein expression profiles in search of overexpressed proteins in murine macrophage cells (RAW264.7) which infected with Bacillus anthracis spores as potentially novel molecular targets. Two differentially expressed proteins were identified in infected murine macrophage cells as Syndapin and CDC46, respectively. Syndapins are potential links between the cortical actin cytoskeleton and endocytosis. Other two proteins were identified from murine macrophage cells infected with avirulent spores as ITBG-2 (CD18) and HSPA5, respectively. These data demonstrate the feasibility of using a MALDI-TOF platform to generate protein expression profiles and identify potential molecular targets for anthrax therapeutics.

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Phage Particles as Vaccine Delivery Vehicles: Concepts, Applications and Prospects

  • Jafari, Narjes;Abediankenari, Saeid
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.18
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    • pp.8019-8029
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    • 2016
  • The development of new strategies for vaccine delivery for generating protective and long-lasting immune responses has become an expanding field of research. In the last years, it has been recognized that bacteriophages have several potential applications in the biotechnology and medical fields because of their intrinsic advantages, such as ease of manipulation and large-scale production. Over the past two decades, bacteriophages have gained special attention as vehicles for protein/peptide or DNA vaccine delivery. In fact, whole phage particles are used as vaccine delivery vehicles to achieve the aim of enhanced immunization. In this strategy, the carried vaccine is protected from environmental damage by phage particles. In this review, phage-based vaccine categories and their development are presented in detail, with discussion of the potential of phage-based vaccines for protection against microbial diseases and cancer treatment. Also reviewed are some recent advances in the field of phagebased vaccines.

Targeting Tumor Metastasis by Regulating Nm23 Gene Expression

  • Prabhu, V. Vinod;Siddikuzzaman, Siddikuzzaman;Grace, V.M. Berlin;Guruvayoorappan, C.
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.8
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    • pp.3539-3548
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    • 2012
  • The Nm23 gene is a metastatic suppressor identified in a melanoma cell line and expressed in different tumors where their levels of expression are associated with reduced or increased metastatic potential. Nm23 is one of the over 20 metastasis suppressor genes (MSGs) confirmed in vivo. It is highly conserved from yeast to human, implying a critical developmental function. Tumors with alteration of the p53 gene and reduced expression of the Nm23 gene are more prone to metastasis. Nm23-H1 has 3'-5' exonuclease activity. This review focuses on the role of Nm23 in cancer progression and also a potential novel target for cancer therapy.

On-farm Tree Planting and Management Guidelines for Medium to High Potential Areas of Kenya

  • Makee, Luvanda A.
    • Journal of Forest and Environmental Science
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    • v.32 no.4
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    • pp.392-399
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    • 2016
  • This review paper presents guidelines which stakeholders use in addressing on-farm tree planting configuration, establishment, tending, silvi- cultural management, management of pests and diseases, challenges and opportunities as practiced in the medium to high potential areas of Kenya. The tree planting configurations discussed includes blocks planting (woodlot), boundary, compound planting, home/fruit gardens, trees intercropped or mixed with pasture, trees on riverbanks and roadside. Participatory monitoring and evaluation techniques have been highlighted. The main challenges facing tree planting activities include culture and attitude of local people, land and tree tenure, inadequate technical support, lack of recognition and integration of technical information and indigenous knowledge, capital and labour shortages, lack of appropriate incentives measures, damage by domestic and wild animals, conflict over trees on the boundary and policy and legal issues. This guideline targets forest managers, extension agents, students and other practitioners in policy and day to day decision making processes in Kenya.

Effects of Water Circulation on the Phosphorus Release Rate from Sediments in the Lake (호수의 물 순환이 저니의 인 용출율에 미치는 영향)

  • Kim, Geonha;Jeong, Woohyeok;Choi, Seunghee
    • Journal of Korean Society on Water Environment
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    • v.21 no.6
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    • pp.595-601
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    • 2005
  • In this research, effects of water circulation on phosphorus release from sediment into water body were studied. Sediments sampled at the Daechung Lake were used for the column experiments with circulation and non-circulation conditions. Deaeration coefficient, $K_1$ and reaeration coefficient, $K_2$ of non-circulation condition were 0.133 and 0, respectively, while $K_1$ and $K_2$ for circulation condition were 0.46 and 0.018, respectively. Oxidation Reduction Potential (ORP) showed a linear relationship with dissolved oxygen (DO) when DO is over 2 mg/L. Phosphorus concentration induced by phosphorus release from sediment was highly dependent upon DO, ORP, and pH. Under anaerobic condition, phosphorus release rate was higher for $Fe^{2+}$-bounded phosphorus compared to that of $Ca^{2+}$-bounded phosphorus.

Carbon nanotubes-properties and applications: a review

  • Ibrahim, Khalid Saeed
    • Carbon letters
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    • v.14 no.3
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    • pp.131-144
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    • 2013
  • The carbon nanotube (CNT) represents one of the most unique inventions in the field of nanotechnology. CNTs have been studied closely over the last two decades by many researchers around the world due to their great potential in different fields. CNTs are rolled graphene with $SP^2$ hybridization. The important aspects of CNTs are their light weight, small size with a high aspect ratio, good tensile strength, and good conducting characteristics, which make them useful as fillers in different materials such as polymers, metallic surfaces and ceramics. CNTs also have potential applications in the field of nanotechnology, nanomedicine, transistors, actuators, sensors, membranes, and capacitors. There are various techniques which can be used for the synthesis of CNTs. These include the arc-discharge method, chemical vaporize deposition (CVD), the laser ablation method, and the sol gel method. CNTs can be single-walled, double-walled and multi-walled. CNTs have unique mechanical, electrical and optical properties, all of which have been extensively studied. The present review is focused on the synthesis, functionalization, properties and applications of CNTs. The toxic effect of CNTs is also presented in a summarized form.

Heat Transfer characteristics of distance between impinging surface and a plane jet (평면제트와 충돌면과의 거리변화에 따른 열전달 특성)

  • 김동건
    • Journal of Advanced Marine Engineering and Technology
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    • v.22 no.5
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    • pp.588-594
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    • 1998
  • Heat transfer characteristics of distance between impinging surface and a plane jet were experi-mentally investigated. The local heat transfer coefficients were measured by a thermochromic liq-uid crystal(TLC) The jet Reynolds number studied was varied over the range from 10,000310 to 30,000310 the nozzle-to-plate distance (H/B) from 4 to 10. It was observed that the Nusselt number increases with Reynolds number the occurrence of the secondary peak in the Nusselt number is within the potential core region the potential core of the jet flow can reach the impinging surface so that the wall jet can a transition from laminar to turbulent flow resulting in a sudden increase in the heat transfer rate.

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Assessment of lactogenic potential of some traditional herbs

  • Raval, M.A.;Patel, H.P.;Mishra, S.H.
    • Advances in Traditional Medicine
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    • v.10 no.2
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    • pp.123-133
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    • 2010
  • The entire plant of Leptadenia reticulata (Asclepidaceae) is extensively used as lactogen, traditionally, in veterinary practice. The plants of Dregea volubilis and Pentatropis microphylla (Asclepidaceae) are now used as its substitute and sometimes replace the original drug as traditional lactogen. The lactogenic potential of these drugs was studied in rats using, pup weight, weight of mother, parenchyma percentage, secretary rating, estimation of total protein content and glycogen content of mammary glands tissues as assessment parameters. HPTLC profiles of bioactive extracts were also generated to serve the authentification needs. The results of present studies show that P.microphylla forms a better substitute over D. volubilis.

Mulberry Handmade Paper Fashion Design with Embedding and Paper Casting Technique (닥 섬유 수제지 의상 디자인에 관한 연구 -임베딩과 페이퍼 캐스팅 기법을 중심으로-)

  • Lee Seung-Ok
    • Journal of the Korea Fashion and Costume Design Association
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    • v.7 no.3
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    • pp.7-15
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    • 2005
  • Culture industry is appearing as an important sector of economy. Many kinds of culture industry like movie, music, drama, animation and game are creating enormous wealth all over the world. Fashion is a kind of culture industry too and even sometimes treated as art. Korean fashion is not treated as real culture but still as a part of textile industry. Internationally Korean fashion has not yet much to show, and despite of it's potential it does not attract much interest from other countries. In this paper properties and effects of mulberry handmade paper clothes were investigated with five clothes made of it. In making handmade mulberry paper clothes various techniques could be applied and these techniques could bring new effects. Because mulberry handmade paper does not have little flexibility than ordinary texture, much efforts should be put to the detail works. Handmade mulberry paper clothes have enormous potential as art, because various approach could be applied.

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Factors related to Performance of Reverse Osmosis Membrane in Seawater Desalination Process (해수담수화 공정에서 역삼투막의 거동에 영향을 주는 요인)

  • Park, Jun-Young;Hong, Sung-Ho;Kim, Ji-Hoon;Jeong, Woo-Won;Nam, Jong-Woo;Kim, Young-Hoon;Lee, Chang-Ha;Kim, Hyung-Soo
    • Journal of Korean Society of Water and Wastewater
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    • v.25 no.2
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    • pp.171-176
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    • 2011
  • Organic matters that comprise a tiny part of seawater generally occur over 50% of membrane fouling in Reverse Osmosis Process. This study evaluates Foundation efficiency of reverse osmosis membranes under brackish and seawater conditions and resistance of organic fouling. Moreover, analyzing the membrane surface through roughness, contact angle and zeta potential results in roughness and contact angle are proportional to flux decline rate (FDR), yet FDR has high value when zeta potential is low level. Furthermore, with various membrane fouling of different raw water conditions, the flux tends to improve when pH value is high and raw water which is complex with organic and cation pollutes membrane faster than organic separated raw water condition.