• Korean Journal of Environmental Biology
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• v.25 no.1
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• pp.1-7
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• 2007

Medaka(Oryzias latipes) and earthworm(Eisenia fetida) toxicity tests were carried out and ecological risk assessment in water and soil was performed with national monitoring data. NOEC of alachlor was $100\;{\mu}g\;L^{-1}$ in the medaka early life-stage test. Embryonic development, hatchability and time to hatching of medaka eggs were affected by this chemical. The $LC_{50}$ and NOEC of alachlor were $94.1\;mg\;kg^{-1}\;and\;55.0\;mg\;kg^{-1}$, respectively, in the earthworm acute toxicity test. The environmental monitoring has been carrying out by NIER since 1999. Exposure levels of alachlor in water and soil were $ND{\sim}0.54\;{\mu}g\;L^{-1}\;and\;ND{\sim}0.9\;{\mu}g\;kg^{-1}$, respectively, in national monitoring data which had been performed from 2000 to 2004. The measured water and soil exposure levels were applied to evaluate the environmental risk assessment. The PNEC of alachlor in water and in soil were determined as $1\;{\mu}g\;L^{-1}\;and\;55.0\;{\mu}g\;kg^{-1}$, respectively using the safety factors which were suggested in EU and OECD. The HQs (PEC/PNEC) were determined to be below 1 for both water and soil when the maximum exposure levels ($0.54\;{\mu}g\; L^{-1}$ in water and $0.9\;{\mu}g\;kg^{-1}$ in soil) were applied. Conclusively, our study indicated that there was not significant ecological risk of alachlor in water and soil of our monitoring sites.

• The Korean Journal of Pesticide Science
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• v.8 no.4
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• pp.309-318
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• 2004

Acute toxicity test and chronic toxicity test were conducted with killifish (Oryzias latipes, Medaka) to evaluate toxicity effect of pesticides. Acute toxicity test was investigated mortality in 48 hours and 96 hours after treatment, chronic toxicity test was examined with the early life stage of 30 days after hatching be started embryos of Medaka. The test substances were two pesticides, Carbosulfan and Phenthoate, applied to the paddy rice plant and well-known to the high fish toxicity. As the result of acute toxicity test, median concentration $(LC_{50})$ at 96 hours in Medata was Carbosulfan 0.102 mg/L and Phenthoate 0.167 mg/L, and Fish early life stage toxicity test was conducted on basis of the result of acute toxicity test and concluded from the investigation of hatching success, period of hatching, survival post hatching, length and weight of surviving fishes and abnormal fish. The results of early life stage toxicity test were represented by no observed effect concentration (NOEC), lowest observed effect concentration (LOEC) and maximum acceptable toxicant concentration (MATC). NOEC was Carbosulfan 0.0067ppm and Phenthoate 0.011ppm, LOEC of PCP-Na, Carbosulfan and Phenthoate were 0.017ppm and 0.029ppm, MATC of Carbosulfan and Phenthoate were 0.011ppm and 0.018ppm. These studies will be expected to supply more varied chronic toxicity effects at lower concentration than acute toxicity test. Therefore, evaluation data will be more realistic and the risk assessment of pesticide will be leveled up.

• The Korean Journal of Pesticide Science
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• v.18 no.1
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• pp.8-13
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• 2014

This study evaluated in vitro cytotoxicity of 5 pesticides, including 2 herbicides, 2 germicides, and an insecticide, as an alternative to the fish acute toxicity test. The in vitro cytotoxicity was tested using a neutral red uptake (NRU) assay with epithelioma papulosum cyprini (EPC) cells that originated from the epidermal tissue of Cyprinus carpio (common carp). An in vivo fish acute toxicity test was conducted according to OECD Test Guideline No. 203 using Aphyocypris chinensis (Chinese bleak), Oryzias latipes (Japanese medaka), and C. carpio. The results showed that the sensitivity of the cell viability assay for the pesticides was similar to the fish acute test in ranking order despite having approximately 10 times less absolute sensitivity. The $r^2$ correlation values were calculated as 0.38 (p = 0.26), 0.76 (p = 0.05) and 0.90 (p = 0.01) for A. chinensis, O. latipes, and C. carpio, respectively. These results suggested that the potential of EPC cell viability assay as an alternative to the fish acute toxicity test due to their good correlation and NRU assay is expected to serve as a useful tool for predicting acute fish lethality for pesticides if further studies with a large set of pesticides are conducted.

• Korean Journal of Environmental Agriculture
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• v.6 no.1
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• pp.50-60
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• 1987

Sensitivity, expressed in the $96h-LC_{50}$ determined with continuous flow system, was compared for eight developmental stages of Oryzias latipes with butachlor, diazinon and fenitrothion. Significance of the laboratory determined $96h-LC_{50}$ in real field situation was evaluated for butachlor using outdoor model agrosystem. In the laboratory tests, the most sensitive stage was post-larva stage IV(7 days after hatching) with $96h-LC_{50}$ of 0.14, 1.4, and 1.6ppm for butachlor, diazinon and fenitrothion, respectively, whereas the least sensitive stage was early embryo stage(1 day after fertilization), and then the susceptibility was somewhat stable after 21days after hatching. In the model agrosystem test, the highest concentration of 0.53ppm of butachlor in water was obtained at the 2nd day after application. The stages IV and VIII showed mortality of over 50% at 96 hours after application. It is concluded that the conventional use of butachlor in the field would cause significant adverse effect on O. latipes. It is suggested that sensitivity test at the early life stages with fish is an important part of the ecotoxicological assessment of chemicals.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.5
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• pp.378-382
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• 2000

The effects of bisphenol A (BPA), known as one of the endocrine disrupting chemicals (EDCs), on the hatching of fertilized eggs (FE) and the spawning of adult fish in songsari, Oryzias latipes were investigated. In condition of the experimental groups, incubated water temperature was $25.0{\pm}0.5^{\circ}C$ and photoperiod was 16 h (light) : 8 h (dark). fE were exposed to aqueous solutions of BPA at nominal concentration of 50, 100 and $200 {\mu}g/l$. The time required in hatching of FE was long in the BPA $50 and 100 {\mu}g/l$ treatment groups when compared to the controls, and in the BPA $200 {\mu}g/l$ treatment group, FE failed to hatch. And also hatching rate was decreased in the BPA treatment groups in comparison to the controls, Adult fishes were reared to oral administration via a diet of 50, 100 and $200 {\mu}g/g$ body weight BPA concentration for 3 weeks, Number of total eggs spawned in the adult fish were fewer in the BPA 100 and 200 treatment groups than in the controls and BPA 50 treatment group. Frequency of abnormal eggs in the total eggs spawned was 11.8 and $16.2{\%}$ in the control and acetone carrier control lower than 36.8, 46,8 and $74.1{\%}$ in the BPA 50, 100 and 200 treatment groups, respectively. And hatching rate of FE decreased in the BPA treatment groups in comparison to the controls. In these results, inhibition of the hatching of FE and the quantity and quality of spawned eggs in adult fish were observed from BPA treatment groups when compared to the controls.

• Environmental Analysis Health and Toxicology
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• v.14 no.1_2
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• pp.55-63
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• 1999

Endocrine disrupting chemicals probably cause the cytological or/and morphological changes of germinal cells in gonad. Accordingly, this study was aimed to make sure that the effect of hormone-mimicking chemicals on gonad morphology such as decrease of germinal cells, inhibition of cellular maturation and change in the ratio of germinal cells in the different developmental phase can be observed by histopathological procedures and can be a useful bio-indicator for the evaluation of endocrine disruption by environmental chemicals. In this experiment, female Japanese medaka were exposured to quercetin, a phytoestrogen, at the concentration of 100 $\mu\textrm{g}$/L. quercetin showed the significant decrease in the number and rate of vitellogenic follicular oocytes in the treated group for 4 and 6 weeks. The weak development of yolk could be also observed. We could conclude that quercetin has anti-estrogenic or androgen-like potency by exerting the inhibition effect on oogenesis in fish female- gonad. From the result of this study, the applied methods and techniques can be evaluated to be a useful biomonitoring means for water pollution, expecting a good result of the subsequent study on apoptosis.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2003.05a
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• pp.156-157
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• 2003

The goal of this study is to develop a biomarker used in monitoring abnormal behaviors of Japanese medaka (Oryzias latipes) as a model organism caused by hazardous chemicals. Japanese medaka was treated under appropriate sublethal concentrations of copper and the fish were subjected to copper treatment after starvation for 48 hr. The untreated individuals showed common behavioral characteristics (i.e., smooth and linear movements with small curvatures) in the movement behaviors.

• Journal of the Korean Applied Science and Technology
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• v.20 no.3
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• pp.204-211
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• 2003

Poly(oxypropylene-oxyethylene glycol) block copolymer(PBC) oil dispersant, which has low toxicity, high biodegradability, and an excellent dispersion efficiency to crude oils and weathered W/O emulsion was prepared by blending PBC, poly(oxyethylene) oleate, and sorbitan monooleate. The dispersing efficiency was measured by swirling flask method. The PBC oil dispersant had an excellent dispersing efficiency to weathered oil products formed as stable W/O emulsion, and the low toxicity, such as 4000 ppm to Oryzias Latipes(24 hr, TLM), Brine Shrimp Artemia(24 hr, TLM).

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2004.12a
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• pp.127-131
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• 2004

The toxicity of 54 selected process effluents of 16 industry classifications in Korea was evaluated by bioassay test using fish Oryzias latipes, invertebrate Daphnia magna, algae Selenastrum capricornutum and bacteria Vibrio fischeri. Our results demonstrate that the toxicity assessment, such as bioassay test, is effective and of practical use for industrial wastewater management in 54 selected effluents with the limitation of the physico-chemical permit. Among 54 effluents, 4 sample failed physico-chemical permit limitation and 50 passed it. The physcio-chemical measurement items and permit concentrations on the present Water Quality Conservation Act are low related a ecotoxicity.

• Environmental Analysis Health and Toxicology
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• v.22 no.3
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• pp.197-202
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• 2007

구리는 환경에 광범위하게 존재하며, 생물체에게 필수적인 무기질이지만 고농도로 존재할 경우 독성을 발휘한다. 본 연구는 프로티옴 기술을 응용하여 수서태계에 구리와 같은 중금속의 존재 여부를 신속하게 평가하기 위한 생물지표를 발굴하기 위하여 수행되었다. 즉, 송사리(Oryzias latipes)를 이용하여 여러 농도의 구리용액(0.1, 1, 5 mg/L)에 24시간 노출시킨 다음, 머리부분에서 선택적으로 발현이 증가되는 단백질을 동정하고자 시도하였다. 본 시스템에서 유의적으로 발현이 증가하는 것으로 나타난 단백질은 beta-tubulin, heat shock cognate 70 (hsc70)이었으며, 이 결과의 일부를 semi-quantitative RT-PCR를 이용하여 확인하였다. 이와 같이 구리 처리에 특이적으로 발현이 증가된 송사리 단백질들은 노출평가를 위한 생물지표로서 개발을 위하여 더 연구할 가치가 있는 것으로 평가된다.