• Title/Summary/Keyword: origin of species

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Origin of lactic acid bacteria in mulkimchi fermentation

  • Hwang, Chung Eun;Haque, Md. Azizul;Hong, Su Young;Kim, Su Cheol;Cho, Kye Man
    • Journal of Applied Biological Chemistry
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    • v.62 no.4
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    • pp.441-446
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    • 2019
  • The assortment of endophytic lactic acid bacteria (LAB) in kimchi derives from its raw vegetables, which include Chinese cabbage, radish, welsh onion, onion, garlic, red pepper, and ginger. These vegetables were examined during mulkimchi fermentation using gene-specific multiplex polymerase chain reaction and 16S ribosomal RNA sequence analysis. Sixteen species from five LAB genera (Leuconostoc, Lactobacillus, Lactococcus, Pediococcus, and Weissella) appeared in the raw kimchi materials. Interestingly, nine LAB species were identified in mulkimchi on fermentation day 0 as follows: Leuconostoc carnosum, Leuconostoc citreum, Leuconostoc gelidum, Leuconostoc inhae, Leuconostoc mesenteroides, Lactobacillus plantarum, Lactobacillus sakei, Lactococcus lactis, and Weissella confusa. Seven additional LAB species were present in mulkimchi at fermentation day 9 as follows: Leuconostoc gasicomitatum, Leuconostoc kimchii, Lactobacillus brevis, Lactobacillus curvatus, Lactobacillus pentosus, Pediococcus pentosaceus, and Weissella koreensis. These species corresponded completely with the LAB in kimchi vegetables. Wei. confusa was the predominant LAB during early fermentation (pH 6.20 to 4.98 and acidity 0.20 to 0.64%), while Lac. sakei, Lac. plantarum, and Wei. koreensis became dominant later in fermentation (pH 4.98 to 3.88 and acidity 0.64 to 1.26%). These results collectively demonstrate that the LAB involved in mulkimchi fermentation originates from the raw vegetables examined.

한라산자생의 왕벚 및 추정양친에 관하여

  • 한창열
    • Journal of Plant Biology
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    • v.7 no.2
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    • pp.14-16
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    • 1964
  • The origin of cultivated Prunus yedoensis Matsumura has been controvertial among botanists. This plant has been reported grown wild in Mt. Hanla. During the crossing experiment on the P. yedoensis and its putative parent performed from April 7 to 19 in Mt. Hanla, the writer studied morphological and physiological characteristics of various species of Prunus and assumed that the wild P. yedoensis may be a hybrid between P. subhirtella var. pendula form. ascendens and P. donarium.

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Pan-Genome Analysis Reveals Origin Specific Genome Expansion in Enterococcus mundtii Strains

  • Neeti Pandey;Raman Rajagopal;Shubham Dhara
    • Microbiology and Biotechnology Letters
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    • v.52 no.2
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    • pp.163-178
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    • 2024
  • Pan-genome analysis is used to interpret genome heterogeneity and diversification of bacterial species. Here, we present pan-genome analysis of 22 strains of Enterococcus mundtii. The GenBank file of E. mundtii strains that have been isolated from different sources i.e., human fecal matter, soil, leaf, dairy products, and insects was downloaded from National Center for Biotechnology Information (NCBI) database and analyzed using BPGA-1.3.0 (Bacterial Pan Genome Analysis) pipeline. Out of a total, 4503 gene families, 1843 belongs to the core genes whereas 1,762 gene families represent the accessory genes and 898 gene families depict the unique genes among all the selected genomes. Majority of the core genes belongs to the categories of Metabolism (37.83%) and Information storage & processing (29.84%) whereas unique genes belongs to the category of Information storage & processing (48.08%). Further, accessory genes are almost equally present in both functional categories i.e. Information storage & processing and Metabolism (34.34% and 32.27% respectively). Further, subset analysis on the basis of the origin of isolates exhibits presence and absence of exclusive gene families. The observation suggests that even closely related strains of a species show extensive disparity in genome owing to their ability to adapt to a specific environment.

Isolation and Identification of Weissella kimchii from Green Onion by Cell Protein Pattern Analysis

  • Kim, Tae-Woon;Lee, Ji-Yeon;Song, Hee-Sung;Park, Jong-Hyun;Ji, Geun-Eog;Kim, Hae-Yeong
    • Journal of Microbiology and Biotechnology
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    • v.14 no.1
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    • pp.105-109
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    • 2004
  • This study was conducted to investigate the potential origin of Weissella species, which were found in ingredients of kimchi, such as salted Chinese cabbage, radish, green onion, red pepper powder, pickled shrimps, garlic, and ginger. Ten strains of Weissella species (Weissella thailandensis, W. kimchii, W. koreensis, W. minor, W. halotolerans, W. hellenica, W. kandleri, W. confusa, W. viridescens, and W. paramesenteroides) and lactic acid bacteria isolated from ingredients of kimchi were analyzed by SDS-PAGE of whole-cell proteins. Several strains with patterns identical to those of Weissella kimchii were isolated from green onion. On the basis of biochemical characteristics and 16S rDNA sequence comparisons, these strains were identified as Weissella kimchii, suggesting green onion as a major origin of Weissella kimchii found in kimchi.

Comparison of Volatile Components in Essential Oil from Different Origin of Atractylodes spp.

  • Chung, Hae-Gon;Bang, Kyung-Hwan;Bang, Jin-Ki;Lee, Seong-Eun;Seong, Nak-Sul;Cho, Joon-Hyeong;Han, Byung-Seok;Kim, Seong-Min
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.2
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    • pp.149-153
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    • 2004
  • This study was carried out to compare the major volatile components in essential oil from different origin of Atractylodes spp. which is being traded as a crude herbal drug in Korean herbal markets. From the two Atractylodes of major volatile components were similarly detected such as the ${\beta}-selinene,\;{\beta}-sesquiphellandrene$, germacrene B, 2,7-dimethoxy-2-methylnaphthalene and 9-methoxy-2,3-dihydrofuro{3,2-q}coumarin. Among the volatile components, the major components were 2,7-dimethoxy-2-methylnaphthalene (40.98%), 9-methoxy-2,3-dihydrofuro {3,2-q} coumarin (15.74%), and ${\beta}-sesquiphellandrene$ (1.98%) in both Atractylodes. As a results, It was found that the two Atractylodes were the same species which was being traded in the Korean herbal markets as the A. japonica. not to different species of A. japonica and A. macrocephalla, respectively.

The brief review on Coal origin and distribution of rare earth elements in various Coal Ash Samples

  • Ramakrishna, Chilakala;Thenepalli, Thriveni;Nam, Seong Young;Kim, Chunsik;Ahn, Ji Whan
    • Journal of Energy Engineering
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    • v.27 no.2
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    • pp.61-69
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    • 2018
  • Rare earth elements together with Y and Sc (REEs) are essential in the development of technology for clean and efficient use of energy. In recent years coal deposits have much attention and attracted as a promising alternative raw sources for rare earth elements, not only because the REEs concentrations in many coals or coal ashes are equal to or higher than those found in conventional types of REEs ores but also because of the world wide demand for REEs in recent years has been greater than supply. In the coal ashes, REEs are mainly associated with carbonates, silicates and aluminosilicates in ashes at 800 and $1100^{\circ}C$. These elements are known to be powerful environmental tracers in natural biogeochemical compartments. In this study, to reviewed the REEs originating and distribution patterns in coal ash samples from the bedrock and/or soil weathering that were entrapped by lichens and mosses was investigated. The REEs patterns of different organisms species allowed minor influence of the species to be highlighted compared to the regional lithology.

Physicochemical and Microbiological Characterization of Protected Designation of Origin Ezine Cheese: Assessment of Non-starter Lactic Acid Bacterial Diversity with Antimicrobial Activity

  • Uymaz, Basar;Akcelik, Nefise;Yuksel, Zerrin
    • Food Science of Animal Resources
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    • v.39 no.5
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    • pp.804-819
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    • 2019
  • Ezine cheese is a non-starter and long-ripened cheese produced in the Mount of Ida region of Canakkale, Turkey, with a protected designation of origin status. Non-starter lactic acid bacteria (NSLAB) have a substantial effect on the quality and final sensorial characteristics of long-ripened cheeses. The dominance of NSLAB can be attributed to their high tolerance to the hostile environment in cheese during ripening relative to many other microbial groups and to its ability to inhibit undesired microorganisms. These qualities promote the microbiological stability of long-ripened cheeses. In this study, 144 samples were collected from three dairies during the ripening period of Ezine cheese. Physicochemical composition and NSLAB identification analyses were performed using both conventional and molecular methods. According to the results of a 16S rRNA gene sequence analysis, 13 different species belonging to seven genera were identified. Enterococcus faecium (38.42%) and E. faecalis (18.94%) were dominant species during the cheese manufacturing process, surviving 12 months of ripening together with Lactobacillus paracasei (13.68%) and Lb. plantarum (11.05%). The results indicate that NSLAB contributes to the microbiological stability of Ezine cheese over 12 months of ripening. The isolation of NSLAB with antimicrobial activity, potential bacteriocin producers, yielded defined collections of natural NSLAB isolates from Ezine cheese that can be used to generate specific starter cultures for the production of Ezine cheese (PDO).

Otolith microchemistry reveals the migration patterns of the flathead grey mullet Mugil cephalus (Pisces: Mugilidae) in Korean waters

  • Bae, Seung Eun;Kim, Jin-Koo
    • Journal of Ecology and Environment
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    • v.44 no.3
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    • pp.185-195
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    • 2020
  • Background: The flathead grey mullet Mugil cephalus has the widest distribution among mugilid species. Recent studies based on mitochondrial DNA sequences showed that the species comprises at least 14 different groups, three of which occur in the northwest Pacific. We analyzed the otolith microchemistry of M. cephalus at several locations in Korea to improve understanding of migration pattern and population origin. Results: We collected 123 sagittal otoliths from seven locations and determined their concentrations of eight elements (7Li, 24Mg, 55Mn, 57Fe, 60Ni, 63Cu, 88Sr, and 138Ba) using laser ablation inductively coupled plasma mass spectrometry. Mean otolith elemental ratios differed significantly among the locations. The Sr:Ca, Fe:Ca, and Ba:Ca ratios were significantly higher than others, and useful chemical signatures for investigating the habitat use of M. cephalus populations. We identified five diverse and complicated migration patterns using the otolith data that we collected: estuarine resident (type I), freshwater migrant (type II), estuarine migrant (type III), seawater resident (type IV), and seawater migrant (type V). A canonical discriminant analysis plot revealed separation of two groups (type II in the Yellow Sea vs. other types in remaining locations). Two locations on Jeju Island, despite their close proximity, had fish with quite different migration patterns, corroborating previous molecular studies that distinguished two groups of fishes. Conclusion: We successfully showed that the migration patterns of the Korean mullet varied by location. Only fish from the western sector of Jeju had a unique migration pattern, which is likely confined population in this area. Among the eight otolith elements measured, the Sr:Ca ratio was found to be the best indicator of migration pattern and population origin.