• Title/Summary/Keyword: origin identification

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Identification of Meat Species Using PCR-RFLP Marker of Cytochrome b Gene

  • Shin, Sung-Chul;Chung, Ku-Young;Chung, Eui-Ryong
    • Food Science of Animal Resources
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    • v.26 no.3
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    • pp.375-379
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    • 2006
  • Food labeling regulations require that the meat species in various meat products are accurately declared to the consumer. Substitution or adulteration of costly meat with a cheaper one is one of the most common problems in the meat industry. In this study, PCR-restriction fragment length polymorphism(RFLP) method of the mitochondrial cytochrome b(mt cyt b) gene has been applied for identification of the origin of six mammalian meat species(beef, port horse, goat, mutton and deer) and three poultry meat species(chicken, turkey and duck) as raw materials for meat products. PCR was used to amplify a variable region of mt cyt b gene. Meat species differentiation was determined by digestion of the amplified products with a 359 bp fragment using HaeIII and HinfI restriction enzymes, which generated species-specific RFLP patterns. This PCR-RFLP DNA marker of mt cyt b gene could be very useful for the accurate and reliable identification and discrimination of animal meat species in routine analysis.

Morphometric Comparison in Rainbow Trout, Oncorhynchus mykiss, Coho Salmon, Oncorhynchus kisutch, and their Allotriploid (무지개송어, 은연어 및 그 잡종3배체의 체형 비교)

  • HONG Kyung-pyo;KIM Pyong-Kih;MYOUNG Jung-Goo;KIM Jong-Man;HUH Hyung-Tack
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.4
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    • pp.687-689
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    • 1997
  • For identification of morphometric characteristics in rainbow trout, Oncorhynchus mykiss, coho salmon, O. kisutch, and their allotriploid, twenty one body traits were measured, and the ratios of each trait to fork length, length from postorbital to the origin of caudal fin and head length were calculated. The ratios of fin length to fin height in anal, dorsal and adipose fins showed significant differences among these two species and their allotriploid $(p\leq0.01)$. Allotriploid could be distinguished easily from two parents species in the ratios of length of origin of ventral fin to fork length and anal fin length to anal fin height.

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Identifying Cluster Candidates in CFHTLS W2 Field

  • Paek, Insu;Im, Myungshin;Kim, Jae-Woo
    • The Bulletin of The Korean Astronomical Society
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    • v.43 no.1
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    • pp.59.2-59.2
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    • 2018
  • Recent studies of galaxy clusters have shown that the galaxy clusters in dense environment tend to have lower star formation rate in local universe with z < 1. However, this correlation is not significant in galaxy clusters with z > 1. The study of galaxy clusters around z=1 can yield insight into cosmological galaxy evolution. Nevertheless, the identification of galaxy clusters beyond the scope of immediate local universe requires wide field data in optical and near-infrared bands. By incorporating data from Canada-France-Hawaii Telescope Legacy Survey(CFHTLS) and Infrared Medium-Deep Survey(IMS), the photometric redshifts of galaxies in CFHTLS W2 field were calculated. Using spatial distribution and photometric redshifts, the galaxies in the field were divided into redshift bins. The image of each redshift bin was analyzed by measuring the number density within proper distance of 1Mpc. By comparing high density regions in consecutive redshift bins, we identified the cluster candidates and mapped the large-scale structure within the CFHTLS W2 field.

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A Case report of Seupon (Shiwen, 습온) with Gyeonggwol (Jingjue, 경궐) (경궐을 동반한 습온 치험 1예)

  • Park Sung-Ho;Song Yun-Kyung;Lim Hyug-Ho
    • The Journal of Korean Medicine
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    • v.25 no.3
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    • pp.203-211
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    • 2004
  • Objectives : We report an unusual case of a 57-year old woman with spasticity following fever of unknown origin (the origin of her fever was not certain by western medical diagnosis). Malignant hyperthemia with spasticity couldn't be cured by general western medical therapy, and furthermore the function of liver and renal system was worsened as a consequence of drugs. Methods : We diagnosed the watery state of the patient as seupon (습온) with gyeonggwol (경궐) through pattern identification (변증) of symptoms and signs. Results : This patient who had spasticity with malignant hyperthermia was treated by optical management of herb medicine and acupuncture, and should need long-term observation. Conclusions : We want to show that trial by febrile diseases can solve fever and spasticity.

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A source and phase identification study of the 10 December 2002 Cheolwon, Korea, earthquake of ML 3.6 (2002년 12월 10일 규모 3.6 철원지진의 진원요소 및 파상분석)

  • 김우한;박종찬;함인경;김성균;박창업
    • Journal of the Earthquake Engineering Society of Korea
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    • v.7 no.5
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    • pp.19-24
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    • 2003
  • We analysed seismic phases recorded by the 10 December 2002 Cheolwon, Korea, earthquake of $M_{L}$ 3.6 and obtained source parameters such as hypocenter, origin time, earthquake magnitude. Velocity and acceleration records used in this study are from the KMA and KIGAM seismic networks. Due to the location of the epicenter in the north of the DMZ(Demilitarized Zone), direct Pg phases were recorded only at five stations in the area south of DMZ. Identification of refracted Pn phase as the first arrival is difficult in most stations. Therefore, the hypocenter determined by existing routine methods could be affected by a large error. In order to avoid the possibility of the problem, we employed a method of seismic phase analysis developed by Kim et al.. The direct, refracted, and reflected P and S phases were successfully identified using the method together with the travel time curve data. In order to improve the accuracy in determination of the hypocenter and origin time, we included PmP and SmS phases in the analysis in addition to the phases such as Pg, Pn, Sg and Sn. The epicenter, depth, and origin time of the Cheolwon earthquake determined based on data of 11 stations within 200km from the epicenter are $38.81^{\circ}$N, $127.22^{\circ}$E, 12.0km, and 7:42:51.4(local time), respectively. The average value of the local magnitude based on the Richter's definition from all the stations is 3.6 in $M_{L}$. This magnitude is smaller by 0.2 and 0.5 compared with magnitudes determined by KMA and KIGAM, respectively.

Molecular and Morphological Identification of a Muraenichthys gymnopterus (Ophichthidae: Anguilliformes) Leptocephalus Collected on Jeju island, Korea (한국 제주도에서 채집된 갯물뱀(뱀장어목: 바다뱀과) 엽상자어(Leptocephalus)의 형태 및 분자동정)

  • Ji, Hwan-Sung;Kim, Jin-Koo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.45 no.5
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    • pp.507-512
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    • 2012
  • One leptocephalus (TL 59.9 mm) from the family Ophichthidae collected from Jeju Island, Korea, was identified using morphological and molecular methods. Our ophichthid leptocephalus was identified as belonging to the genus Muraenichthys based on morphological characters: 157 myomeres; the origin of the dorsal fin located a little in front of the anus; a distinct melanophore present on the opercle; and six gut swellings present. An analysis of 886 base pairs of the 12S rRNA mtDNA sequences showed that our leptocephalus must be Muraenichthys gymnopterus, because its sequences were concordant with those of an adult M. gymnopterus (d=0.001) and next to those of Muraenichthys sp. leptocephalus (d=0.034). Here, we are the first to describe the morphological characteristics of the M. gymnopterus leptocephalus.

Studies on the Malic Acid Degradation in Wine by Yeast (Part 1) Isolation and Identification of Yeast Strain (효모에 의한 과실주중의 감산효과에 관한 연구 (제1보) 균주의 분리 및 동정)

  • 유대식
    • Microbiology and Biotechnology Letters
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    • v.6 no.1
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    • pp.23-26
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    • 1978
  • Yeast strains capable to perform malo-alcoholic fermentation in wine were screened. Out of 54 strains isolated from apples, tomatos, grapes, and strawberries, two strains showed strong assimilation of malic acid. After further screening one strain of strawberry origin was selected and identified as Schizosacch-aromyces japonicus var. japonicus based on the morphological and physiological characteristics examined.

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A Search for Endogenous Digitalis-like Factors (인체 및 고등 동물에서 생성되는 디기탈리스 유사체의 탐색)

  • 송영순
    • YAKHAK HOEJI
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    • v.30 no.4
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    • pp.198-201
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    • 1986
  • The successful elucidation of the opiate receptor and its natural ligands has generated speculation that other drugs might interact with cellular sites designed to accommodate endogenous mediators. The properties of binding sites for the cardiac glycosides(CG), together wit the potency and specificity of the digitalis drugs suggest that CG mimic an endogenous digitalis-like factor. Recenthy, several laboratories have reported the potency in mammalian tissues and fluids of unidentified materials that share certain specific propeties of the CG. Identification of body's own digitalis might yield a natural co pound whose synthetic analogs may provide safer and more effective drugs than can be achieved by structural edification of the CG of plant origin.

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Identification of the geographical origin of cheonggukjang by using fourier transform near-infrared spectroscopy and energy dispersive X-ray fluorescence spectrometry (근적외선분광분석기 및 에너지 분산형 X선 형광분석기를 이용한 청국장 원산지 판별)

  • Kang, Dong-Jin;Moon, Ji-Young;Lee, Dong-Gil;Lee, Seong-Hun
    • Korean Journal of Food Science and Technology
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    • v.48 no.5
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    • pp.418-423
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    • 2016
  • This study was conducted to identify the geographical origin of soybeans in Cheonggukjang by analyzing its organic components and inorganic elements with Fourier transform near-infrared spectroscopy (FT-NIRS) and with energy dispersive X-ray fluorescence (ED-XRF) coupled with multivariate statistical analysis. For method development, 280 samples from various regions were collected and analyzed. The discriminant accuracy for the developed methods was 97.5% for FT-NIRS and 98.0% for ED-XRF with multivariate statistical analysis. A validation test confirmed the discriminant accuracy to be 96.3% for FT-NIRS and 95.0% for ED-XRF. Overall, the results showed that methods using FT-NIRS and ED-XRF could be used to identify the geographical origin of Cheonggukjang.