• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2010년도 심포지엄 및 추계학술발표회
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• pp.165-166
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• 2010

• Journal of Applied Biological Chemistry
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• 제56권1호
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• pp.23-27
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• 2013

순무뿌리(Brassica rapassp)를 실온에서 95% ethanol 수용액으로 추출하고 이 추출물을 ethyl acetate (EtOAc)분획, n-butyl alcohol 분획 및 $H_2O$ 분획으로 나누었다. EtOAc분획에 대하여 silica gel, ODS 및 Sephadex LH-20 column chromatography를 반복실시 하여 5종의 flavonoid를 분리하였다. NMR, IR 및 MS data를 해석하여 각각 licochalcone A (1), 4,4'-dihydroxy-3'-methoxychalcone (2), liquiritigenin (3), liquiritin (4), isoliquiritin (5)으로 구조동정하였다. 이들 화합물들은 순무뿌리에서는 처음으로 분리되었다.

• Journal of Applied Biological Chemistry
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• 제57권1호
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• pp.29-32
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• 2014

This study was initiated to search for the most effective method for extraction of anthocyanins from Morus alba Fruits using organic acids and to evaluate the stability of the pigments at various storage environments. The anthocyanins were effectively extracted by addition of organic acids such as citric acid, malic acid and fumaric acid. The anthocyanins were not degraded at $25^{\circ}C$ and under a fluorescent light with 183 lux, but sharply degraded at $60^{\circ}C$.

• 생약학회지
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• 제40권3호
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• pp.178-183
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• 2009

In DPPH radical and nitric oxide scavenging ability, the extract of lowest temperature condition(40-15H ; 15 hours at $40^{\circ}C$ for drying) exhibited highest activity. The total phenolic and flavonoid contents of each extracts were found to be $120.4{\sim}193.3\;mg$/g and $86.91{\sim}94.55\;mg$/g respectively. It is shown that 40-15H was the highest content in each compound. In antimicrobial activity, a lower drying temperature conditions were found to be more strong activities in disc diffusion assay and each extracts showed MIC of identical level against every tested microbial strains. However 100-2H(2 hours at $100^{\circ}C$ for drying) was exhibited MIC of slightly low level against some strains. And every extract showed fine cell viabilities($101.7{\sim}122.9%$) against RAW 264.7 cell. In anti-proliferation activity against AGS, each extract showed a similar inhibition activity. However in anti-proliferation activity against HeLa, a lower drying temperature conditions showed more strong activities.

• Plant Resources
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• 제6권3호
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• pp.205-210
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• 2003

Ginseng(Panax ginseng C.A. Meyer) is a perennial herbaceous plant which grows very slowly. It takes about 3 to 4 years from seeding to collecting the ripe seeds and the ginseng propagation is very difficult. and so, it is very difficult to breed ginseng plant. Ginseng tissue culture was started from at 1960, and ginseng commercial product by in vitro callus culture was saled, however upto now, regenerants were not planted to soil normally. Recently, plant genetic engineering to produce transgenic plants by introducing useful genes has been advanced greatly. In a present paper, transformation of ginseng plants was achieved by co-cultivation with Agrobacterium harboring the binary vector coding Proteinase-II gene, which confer resistant or tolerant to insect pests, The binary vector for transformation was constructed with disarmed Ti-plasmid and with double 35S promoter. The NPT II gene and introduced genes of the transgenic ginseng plants were successfully identified by the PCR. Especially the transgenic ginseng plants were regenerated using new techniques such as repetitive single somatic embryogenesis.

• 대한본초학회지
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• 제35권2호
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• pp.39-46
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• 2020

Objective: In this study, we analyzed the trend of patents registered prior to October 4, 2019, to understand the industrial trends in insect-derived medicinal materials used for the treatment of skin diseases. Methods: Using the WIPSON database, we collected information regarding the patents related to insect-derived materials for treating various skin diseases. Results: The patents registered prior to October 4, 2019, from Korea, Japan, the USA, China, and the EU, along with those registered under PCT were selected. There were 195 patents related to the use of insect-derived medicinal materials in treating various skin diseases such as psoriasis, inflammatory skin diseases, eczema, pruritus, and atopic dermatitis. China is mostly superior in total number of registered patents compared with the other countries. Korea was the major patent technology-holder for atopic dermatitis, but China dominated in the remaining categories of skin diseases. Upon first patent registration in 1992, there had been a continual increase in the number of patents. Especially, patents related to eczema, psoriasis, inflammatory skin disease were markedly increased. Most frequently used insect-derived medicinal materials was Scolopendra, Cicadidae Periostracum, Scorpions, Cantharides, and Batryticatus Bombyx. The insect-derived medicinal materials were generally used as a combined preparation with other medicinal materials in patents. Conclusion: This study could help to establish the basis for future research and development related treating skin diseases using insect-derived medicinal materials. In order to provide sufficient data, further study including analysis of rejected patents is needed.

• Journal of Ginseng Research
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• 제38권2호
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• pp.116-122
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• 2014

Ginseng roots were extracted with aqueous methanol, and extracts were suspended in water and extracted successively with ethyl acetate and n-butanol. Column chromatography using the n-butanol fraction yielded four purified triol ginseng saponins: the ginsenosides Re, Rf, Rg2, and 20-gluco-Rf. The physicochemical, spectroscopic, and chromatographic characteristics of the ginsenosides were measured and compared with reports from the literature. For spectroscopic analysis, two-dimensional nuclear magnetic resonance (NMR) methods such as $^1H$-$^1H$ correlation spectroscopy, nuclear Overhauser effect spectroscopy, heteronuclear single quantum correlation, and heteronuclear multiple bond connectivity were employed to identify exact peak assignments. Some peak assignments for previously published $^1H$-and $^{13}C$-NMR spectra were found to be inaccurate. This study reports the complete NMR assignment of 20-gluco-Rf for the first time.

• Journal of Applied Biological Chemistry
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• 제56권4호
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• pp.213-217
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• 2013

Phytochemical studies on the leaves of Ailanthus altissima (Simaroubaceae) have not been reported previously. Thus, the authors isolated and identified secondary metabolites from A. altissima. Dried and powdered leaves were extracted with 80% aqueous methanol, and the concentrated extract was successively partitioned with ethyl acetate, n-butanol, and water. Four flavonoids were isolated from the ethyl acetate fraction through repeated silica gel and octadecyl silica gel column chromatography. Spectroscopic data including NMR, MS, and IR allowed for identification of the chemical structures as quercetin (1), afzelin (2), quercitrin (3), and isoquercitrin (4). This is the first report of the isolation of these compounds from A. altissima. The four isolated flavonoids 1-4 as well as solvent fractions (ethyl acetate, n-butanol, and water), were evaluated for DPPH radical scavenging activity.

• Journal of Applied Biological Chemistry
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• 제55권2호
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• pp.103-107
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• 2012

오가피(Acanthopanax sessiliflorus Seeman) 열매 10.0 kg을 실온에서 70% EtOH 수용액으로 추출하고 이 추출물을 ethyl acetate (EtOAC) 분획, n-butyl alcohol 분획, $H_2O$ 분획으로 나누었다. EtOAc 분획에 대하여 silica gel 및 octadecyl silica gel chromatography column chromatography를 반복 실시하여 4종의 화합물을 분리, 정제하였다. NMR, IR, EI/MS, 및 GC/MS 등을 통하여 화합물 1(stigmasterol), 화합물 2(linoleic acid), 화합물 3(${\beta}$-sitosterol), 화합물 4(stigmast-5-en-$3{\beta}$,$7{\beta}$-diol)의 구조를 결정하였다.

• 한국수학교육학회지시리즈A:수학교육
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• 제49권1호
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• pp.15-37
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• 2010

In this study, we investigated the application of oriental history of mathematics in school mathematics teaching. We set up three study problems to achieve this purpose. First, we analyze the middle and high school mathematics textbooks and auxiliary books. Second, we survey the mathematics teacher's knowledge and degree of application on history of mathematics. Third, we develop the teaching and learning materials on oriental history of mathematics. We performed three study-methods to settle above study problem. First, we analyzed 24 textbooks and auxiliary books for study problem 1. There were 6 middle school mathematics textbooks and 6 auxiliary books and also 6 high school mathematics textbooks and 6 auxiliary books. We categorized the contents into "anecdote", "systematization", "application of problem", "expansibility of thought", and "comparative of the contents". Second, we surveyed the 78 mathematics teachers's knowledge and degree of application using questionnaire about knowledge and application on history of mathematics. The questionnaire was made up of four types of question; the effect of material about history of mathematics, the understanding of western history of mathematics, the understanding of oriental history of mathematics; the direction of development of teaching material. Third, we exemplified the teaching and learning materials about three categories: "anecdote", "comparative of the contents".