• The Journal of Korean Medicine
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• v.30 no.6
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• pp.27-34
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• 2009

Objectives: To evaluate the acute toxic effects and approximate lethal dose of Cheonggan extracts (CGX) in SD rats and beagle dogs. Methods: Male and female rats were divided into 4 groups (Control, CGX 1250, CGX 2500, CGX 5000) respectively and male and female dogs were divided into two groups respectively (Control, CGX 5000) respectively. A single oral dose of CGX was treated to the rats and dogs. Mortality, signs of gross toxicity, and behavioral changes were observed over 14 days. All animals were observed every hour for 4 hours after administration and once a day thereafter for 14 days. Body weights were determined at $0_{th}$, $7_{th}$, and $14_{th}$ days. All surviving animals were sacrificed and necrotized. Major organs were inspected visually for gross findings. Results: No animals died in any of the groups during the experimental period (2 weeks), rats or dogs. Body weights of rats and dogs during the experiment continuously increased in all groups but there was no significant change. No abnormal clinical signs were observed for 2 weeks after a single administration of CGX in any dose group of CGX, rats or dogs. No abnormal findings in major organs were observed in any group of rats or dogs. Conclusion: CGX does not have acute toxic effects in rats or dogs. Therefore, an approximate lethal dose is assumed to exceed 5000 mg/kg in both rats and dogs.

• Journal of Acupuncture Research
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• v.19 no.2
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• pp.221-230
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• 2002

Some studies report that electroacupuncture(EA) boost natural killer cell(NK cell) activity. And also it is well known that hypothalamus is deeply related to effects of EA on analgesia. Some reports said that especially lateral hypothalamic area(LHA) is related to splenic NK cell activity. In order to investigate the relation between hypothalamus and effects of EA at ST36 point on NK cell activity of Spraque-Dawley rats. Lesions were made bilaterally at lateral hypothalamic area(LHA). And NK cytotoxities of normal and lesioned rats were measured with 51Cr release immunoassay after EA stimulation for 2 and 14 days. NK cell activity of EA group was significantly higher than sham group at 2nd, 14th day in normal rats. And LHA lesions abolished effects of EA on NK cell activity at 2nd day. But LHA lesions did not affect the effects of EA at 14th days. We also had an evidence that the decrease of NK cell activity was almost recoverd at 14th day. These results strongly suggest that LHA is deeply related to increase of NK cell activity induced by EA.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.11
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• pp.5847-5855
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• 2013

The purpose of this research is to develop Dementia-diagnosis application program through inter-working between biometric sensors and smart phone with diagnostic programs. Receiving data from 2 biometric sensors, SpO2 and HRV(heart rate variability) with Bluetooth and adding scores from questionnaires and memory test game, smart phone can draw a final Dementia Index(DI). Simple clinical tests provided threshold for Dementia, and diagnosis was made by comparing the DI with the threshold. We performed testing on persons aged over 60 and found out the proposed application can be used to diagnose Dementia easily and quickly. Additionally, we have shown the potential of oxygen chamber combined with Dementia-diagnosis application as a medical equipment to ease or cure Dementia in its early stages.

• Herbal Formula Science
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• v.17 no.2
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• pp.123-132
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• 2009

Acetaminophen (AP) is widely used as an over-the-counter analgesic and antipyretic drug. AP-induced hepatotoxicity is a common consequence of AP overdose and may lead to acute liver failure. In this study, we investigated the liver damage in mice using single dose (300 mg/kg) of AP and the possible protective effects of administration (50-200 mg/kg body weight) of Joo-Juk on acetaminophen-induced liver damage in mice. The alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activities were determined in the plasma of mice. The effect of Joo-Juk on lipid peroxidation product thiobarbituric reacting substances (TBARS) and some antioxidant enzymes superoxide dismutase (SOD), catalase, d-aminolevulinate dehydratase ($\sigma$-ALA-D) activities, and gluthathione peroxidase (GPx), were also evaluated in the mouse liver homogenate. AP caused liver damage as evident by statistically significant increased in plasma activities of AST and ALT. There were statistically significant losses in the activities of SOD, catalase, $\sigma$-ALA-D, and GPx and an increase in TBARS in the liver of AP-treated group compared with the control group. However, Joo-Juk was able to counteract these effects. These results suggest that Joo-juk can act as hepato-protectant against AP toxicity and is a good candidate for further evaluation as an effective chemotherapeutic agent.

• Herbal Formula Science
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• v.16 no.2
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• pp.183-191
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• 2008

Acetaminophen (N-acety1-p-aminophenol, paracetamol) is widely used as an over-the-counter analgesic and antipyretic drug. Intake of a over dose of acetaminophen may result in severe hepatic necrosis. In this study, we investigated the liver damage in mice using single dose (300 mg/kg) of acetaminophen and the possible protective effects of administration (50-200 mg/kg body weight) of SB-Ex on acetaminophen-induced liver damage in mice. The alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activities were determined in the plasma of mice. The effect of SB-Ex on lipid peroxidation product thiobarbituric reacting substances (TBARS) and some antioxidant enzymes superoxide dismutase (SOD), catalase, d-aminolevulinate dehydratase (${\sigma}$-ALA-D) activities, and gluthathione peroxidase (GPx), were also evaluated in the mouse liver homogenate. Acetaminophen caused liver damage as evident by statistically significant increased in plasma activities of AST and ALT. There were general statistically significant losses in the activities of SOD, catalase, ${\sigma}$-ALA-D, and GPx and an increase in TBARS in the liver of acetaminophen-treated group compared with the control group. However, SB-Ex was able to counteract these effects. These results suggest that SB-Ex can act as hepatoprotectives against acetaminophen toxicity and is a good candidate for further evaluation as an effective chemotherapeutic agent.

• The Journal of Pediatrics of Korean Medicine
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• v.32 no.2
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• pp.1-10
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• 2018

Objectives This study investigated the effects of Hataedock treatment with Douchi on induction of allergic rhinitis in obese induced NC/Nga mice. Methods NC/Nga mice were divided into control group (Ctrl), allergic rhinitis induced obese mice group (ARE), and allergic rhinitis induced obese mice group with Douchi Hataedock treatment (FGT). The 3-week-old mice of the FGT group were given one 10 mg/kg dose of Douchi Hataedock extract and sensitized with allergic antigens at weeks 4, 5, and 6. After 1 week of final sensitization, allergic rhinitis was induced primarily in mice nasal cavities for five days. After one week of the completion with the first induction, the second induction was introduced by the same method. After 1 week, few samples of the nasal mucosal tissues of each group were prepared. The factor of Th2 differentiation and inflammation control such that IL-4, STAT6, CD40, $Fc{\varepsilon}RI$, substance P, MMP-9, $NF-{\kappa}B$ p65, p-IkB, iNOS and COX-2 were observed by immunohistochemistry. Also, the difference in nasal mucosal injury was observed by histochemical method (PAS staining). Results The FGT group showed that reduced IL-4 production, STAT6 expression and CD40 expression by regulating excessive Th2 differentiation. Also, production of substance P and MMP-9 and activity of $Fc{\varepsilon}RI$ in mast cells were decreased. Inhibition of $NF-{\kappa}B$ p65 activity was induced by inhibition of p-IkB, and the production of inflammatory enzymes iNOS and COX-2 were decreased. In addition, the damage of intramural respiratory epithelium was low and excessive mucin secretion in goblet cells was low. Conclusions This study confirmed the possibility of controlling the allergic rhinitis in obese children who are expected to have an overactive inflammation.

• Journal of Pharmacopuncture
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• v.6 no.1
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• pp.35-36
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• 2003

Background: Evidence based medicine has become main tools for medical practice. However, conducting a highly ranked in the evidence hierarchy pyramid is not easy or feasible at all times and places. There remains a room for descriptive clinical outcome measure studies with admitting the limit of the intepretation. Aims: Presents three Korean clinic based outcome measure studies with a view to encouraging Korean clinicians to conduct similar studies. Methods: Three studies are presented briefly here in치uding 1) Quality of Life of liver cancer patients after 8 Constitutional acupuncture; 2) Developing a Korean version of Measuring yourself Medical Outcome profile (MYMOP); and 3) Survey on 5 Shu points: a pilot In the first study, we have included 4 primary or secondary liver cancer patients collecting their diagnostic X-ray film and clinical data f개m their hospital, and asked them to fill in the European Organization Research and Treatment of Cancer, Quality of Life Questionnaire before the commencement of the treatment. The acupuncture treatment is set up format but not disclosed yet. The translation and developing a Korean version of outcome measures that is Korean clinician friendly has been sought for MYMOP is one of the most appropriate one. The permission was granted, the translation into Korean was done, then back translated into English only based on the Korean translation by the researcher who is bilingual in both languages. The back translation was compared by the original developer of MYMOP and confirmed usable. In order to test the existence of acupoints and meridians through popular forms of Korean acupuncture regimes, we aim at collecting opinions from 101 Korean clinicians that have used those forms. The questions asked include most effective symptoms, 5 Shu points, points those are least likely to use due to either adverse events or the lack of effectiveness, theoretical reasons for the above proposals, proposing outcome measures, and the time from insertion to be effective. The questionnaire is posted on the virtual notice board of the homepage of the communication KOMA (The official tool of Association of Korean Oriental Medicine).

• Journal of Korean Physical Therapy Science
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• v.13 no.3
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• pp.41-54
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• 2006

The purpose of this study was to analyze various forms of musculoskeletal pain during different stages such as antenatal, pregnancy, delivery, and postpartum. The data for this study were collected through a self-administered survey with a structured questionnaire to 113 subjects. The survey was administered from May, 2003 to March, 2004 with subjects selected from postpartum centers in 3 different areas: Kangnam, Kangbook, and Bucheon. Analytical tools used for this study were frequency analysis, crosstabulation, percentage analysis, independent t-test, chi-square, and multiple response analysis. The findings of this study were as follows: 1. Comparison study of musculoskeletal pain according to variables including a pregnant woman's age, delivery techniques, different types of delivery such as primiparity, multiparity, dystocia, easy delivery, shows that these relationships are statistically not significant (p>0.05). 2. A total of 61.9% gave positive responses for the question about the relationship between musculoskeletal pain during postpartum and that in preparation of labor, but shows that this relationship was statistically not significant (p>0.05). 3. Relationships between forms of musculoskeletal pain in different phases are significant (p<0.05). The compared phases are: premarriage and pre-pregnancy, premarriage and antenatal, premarriage and postpartum, premarriage and lactiferous phase, pre-pregnancy and antenatal, pre-pregnancy and postpartum, and pre-pregnancy and lactiferous phase. However, results for the comparison between antenatal and postpartum, antenatal and lactiferous phase, and postpartum and lactiferous phase show that these relationships are statistically not significant (p>0.05). According to this study, musculoskeletal pain which occurred during antenatal is significantly related to the pain occurring during postpartum. Results produced from this study might be used as a helpful tool for developing educational programs aiming at teaching self pain-detection performable at home or at the workplace and body maintenance during the course of antenatal and postpartum.

• The Journal of Churna Manual Medicine for Spine and Nerves
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• v.8 no.1
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• pp.49-61
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• 2013

Objectives: The aim of this study was to analyse the domestic trends of Chuna treatments techniques in Korean literature. Methods: We searched the clinical trials on Chuna treatments through both electronic search(used keyword 'chuna') and hand search in 3 Korean web databases(OASIS, NDSL, RISS) and 4 related journals(The Journal of Korea CHUNA Manual Medicine for Spine & Nerves, Journal of Oriental Rehabilitation Medicine, The Journal of Korean Acupuncture & Moxibustion Society, Journal of Korean Medicine Society). All relevant clinical trials were selected and extracted to be analyzed according to their published year, journals, types of study, used techniques. Results: The number of the clinical studies tends to increase every year. The studies on Chuna treatments were mainly published in The Journal of Korea Chuna Manual Medicine for Spine & Nerves. In case of types of study, case reports and case series were predominant. The most frequently adopted techniques of Chuna in studies were flexion distraction technique for lumbar spine and JS supine position cervical spine distraction. Conclusions: Through the results of this study, we hope that the more qualitative education could be conducted by strengthening the techniques which often used. Also analysis of the reason of rarely used techniques should be conducted and the modification or developing techinques should be followed as a counter measures. As applying more rigorous methodology, more qualitative evidence based Chuna studies should be conducted in future clinical research.

• Journal of Biomedical Engineering Research
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• v.30 no.6
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• pp.469-475
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• 2009

Diseases caused by indurate tissues of the internal organs are liver cirrhosis and abdominal sclerosis. The cause of chronic gastro-intestinal disease is a digestive system disorder and a defecation disorder. They impede peristaltic movement and digestive system with the symptom that indurate tissues. The purpose of the present study was to determine the disease grade quantitatively by measuring an indurated standard of tissues and organs. For the measurement of elasticity, we designed the system that measure the displacement of the substance and approved pressure using ultrasound transducer. For verification of developed system, we compared elasticity as results of experiment between the developed system and public elasticity measurement machine at individual plastic phantoms made by plastic hardener and softener. Elasticity of the plastic phantoms is averagely 0.007MPa lower measured by developed system than Micro-indenter, and less than 10% errors. Comparing with economical value and accuracy between developed system and Micro-indenter, the system is significant of measurement for tissue elasticity. Thus, it is possible to measure a elasticity at human tissue and organ. A chronic gastro-disease as well as grade could be decided objective validity using this system.