• Journal of Acupuncture Research
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• 제27권6호
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• pp.115-122
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• 2010

Objectives : The purpose of this study was to propose the utilization plan of Ryodoraku in the U-health care systems. Methods : Computerized literature searches were performed for Ryodoraku related articles using the following databases: KISS, RISS, DBPIA, NDSL from 1990 to Oct 2010. Search terms were '양도락' or 'Ryodoraku' or 'U-health'. Due to Ryodoraku coming from Japan, additional literature review(articles published by 2008) on Japanese journal of Ryodoraku medicine was done for compensation. Results : 1. Introduction of U-health : As rapid progress of population aging and strong interest in health, the demand for the traditional Korean medical service is increasing. Until now healthcare service has provided post treatment by face-to-face manner. But according to related researches, proactive treatment is resulted to be more effective for preventing diseases. Particularly, the existing healthcare services have limitations in preventing and managing chronic geriatric degenrative diseases such as metabolic syndrome, CVA, coronary heart diseases, parkinson's diseases, degenerative joint disease, spondylosis, etc., because the cause of the above is complex and even related to life habit. As the advent of ubiquitous technology, patients with the chronic geriatric degenrative diseases can improve life habit such as poor eating habits and physical inactivity without the constraints of time and space through u-healthcare service. Therefor, lots of researches for u-healthcare service focus on providing the personalized healthcare service for preventing and managing that. To cope with this situations, The concept of u-healthcare service should be adopted in the traditional korean medicine and diagnostic devices suitable for it should be also devised and developed based on traditonal korean medine. 2. Review of existing Ryodoraku related articles for applying to U-health : articles investigating feasibility applying Ryodoraku to meridian diagnosis and raising problems of it, articles providing recent research trends of Ryodoraku, Ryodoraku related articles considering usefulness for U-health, and articles confirming the repeatability and reproducity of Ryodoraku were included. Based on the review of the above Ryodoraku related articles, several application of Ryodoraku in the U-health care system. Conclusions : To make preparations for the increasing need of traditional Korean medicine due to rising morbidity rate of chronic geriatric degenerative diseases, it is necessary to appropriately apply Ryodoraku to the U-health care system. The application of Ryodoraku is as follows. 1. To use Ryodoraku additionally to the established diagnostic device of metabolic syndrome, CVA, coronary heart diseases, parkinson's diseases, degenerative joint disease, spondylosis. 2. To apply Ryodoraku to the symptoms or diseases having a tendency to be diagnosed by correlation between the affected meridian and the lesion such as headache, nuchal pain, shoulder pain, low back pain, sciatica, HNP, etc. 3. To secure the repeatability and reproducity of Ryodoraku. 4. To devise and develop Ryodoraku appliance in order to overcome the known drawbacks and to improve error of measurement.

• 한방안이비인후피부과학회지
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• 제32권3호
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• pp.13-22
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• 2019

Objectives : Armeniacae semen is the seed of Prunus armenica L. var. ansu MAXIM, and this is classified into Rosaceae. Armeniacae semen has been used for centuries in traditional oriental medicine for the treatment of pain and inflammatory diseases. Amygdalin is the major compound of Armeniacae semen, and it is now being used for the treatment of pain and cancer. Methods : In the present study, we compared the effects of an aqueous extract of Armeniacae semen and a solution of amygdalin extracted from Armeniacae semen on lipopolysaccharide(LPS)-stimulated prostaglandin E2 synthesis and nitric oxide production in mouse BV-2 microglial cells. For this study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay, reverse transcription-polymerase chain reaction(RT-PCR), prostaglandin E2 immunoassay and nitric oxide detection were performed on mouse BV-2 microglial cells. Results : In the present study, an aqueous extract of Armeniacae semen and an amygdalin solution extracted from Armeniacae semen suppressed prostaglandin E2 synthesis and nitric oxide production by inhibiting the LPS-induced enhancement of cyclooxygenase-2(COX-2) mRNA and the inducible nitric oxide synthase mRNA in mouse BV-2 cells. For the cyclooxygenase-1(COX-1) expression, an aqueous extract of Armeniacae semen showed a more potent suppression effect compared to the amygdalin solution. However, the amygdalin solution more potently suppressed the LPS-induced COX-2 mRNA expression compared to the aqueous extract of Armeniacae semen. Conclusions : As a result, aqueous extract of Armeniacae semen and amygdalin exert anti-inflammatory and analgesic effects.

• 동의생리병리학회지
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• 제21권2호
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• pp.387-391
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• 2007

Recently, much attention has been paid to developing various kinds of fermented herbal extracts, a new type of traditional herbal medicine, in the field of Korean traditional medicine. The fermentation of medicinal herbs is intended to exert a favorable influence on digestability, bioavailavility and pharmacological activity of herbal extract in the gastrointestinal tract. It also produces a number of fermentation products that intensify the nutritional and pharmacological aspects of the medicinal herbs. In order to develop a functional beverage of alleviating the aftereffects of the previous drinks, the extracts (HP-1) of fermented herbal mixture, including Artemisia capillaris Thunb., Lonicera japonica Thunberg, and Hovenia dulcis Thunb., were prepared and the medicinal effect as a hangover cure was evaluated in ethanol-loaded rats. The enzyme activities of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase-2 (ALDH2) were analyzed by measuring the concentration of blood ethanol and acetaldehyde. The mRNA expression of ADH and ALDH2 was also investigated through RT-PCR analysis. In the HP-1-treated group, the concentration of blood ethanol was significantly reduced at one hour after loading of ethanol, as compared to that in the saline-treated group. The reduced ethanol was converted to acetaldehyde, which resulted in rapid increase in acetaldehyde concentration in an hour. Acetaldehyde was started to decrease at 5 hours after ethanol loading. It implies that HP-1 is highly effective to stimulate the activities of ADH and ALDH2. The HP-1 treatment also activated the mRNA expression of ADH and ALDH. This study suggests that fermented herbal extract, HP-1 can be used as a functional beverage of alleviating the alcohol-induced hangover symptoms by stimulating the activities and gene expression of hepatic alcohol metabolizing enzymes.

• 대한본초학회지
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• 제36권6호
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• pp.1-8
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• 2021

Objectives : Atractylodis rhizoma Alba has been traditionally used as a medicinal resource that is used for enhancing Qi (氣) in traditional medicine in Korea, China, and Japan. This study investigated the protective effects of Atractylodis rhizoma Alba extract (ARE) against trimethyltin (TMT), a neurotoxin that causes selective hippocampal injury, using both in vitro and in vivo models. Methods : We investigated the effects of ARE on TMT- (5mM) induced cytotoxicity in primary cultures of mouse hippocampal cells (7 days in vitro ) and on hippocampal injury in C57BL/6 mice injected with TMT (2.6 mg/kg). Results : We observed that ARE treatment (0 - 50 ㎍/mL) significantly reduced TMT-induced cytotoxicity in cultured hippocampal neurons in a dose-dependent manner, based on results of lactate dehydrogenase and 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide assays. Additionally, this study showed that orally administered ARE (5 mg/kg; between -6 and 0 days before TMT injection) significantly attenuated seizures in adult mice. Furthermore, quantitative analysis of allograft inflammatory factor-1 (Iba-1)- and glial fibrillary acidic protein (GFAP)- positive cells showed significantly reduced levels of Iba-1- and GFAP-positive cell bodies in the dentate gyrus of mice treated with ARE prior to TMT injection. These findings indicate the significant protective effects of ARE against the TMT-induced massive activation of microglia and astrocytes in the hippocampus. Conclusions : We conclude that ARE minimizes the detrimental effects of TMT-induced hippocampal neurotoxicity, both in vitro and in vivo . Our findings may serve as useful guidelines to support ARE administration as a promising pharmacotherapeutic approach to hippocampal degeneration.

• 생명과학회지
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• 제24권1호
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• pp.92-97
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• 2014

Cordycepin은 Cordyceps militaris에서 처음 유래된 nucleoside adenosine 유도체의 일종으로 면역증강 및 항암활성을 포함한 다양한 약리 기능이 있는 것으로 알려져 있다. 본 연구에서는 LNCap 인체 전립선 암세포 모델을 이용하여 cordycepin에 의한 항암활성 기전을 연구하였다. Cordycepin 처리에 따라 LNCap 세포는 처리 농도 의존적으로 증식이 억제되었으며, 이는 apoptosis 유발과 연관성이 있음을 poly ADP-ribose polymerase의 단편화 현상과 Annexin V 염색에 의한 정량적 분석으로 확인하였다. Cordycepin 처리에 따른 flow cytometric analysis 결과로서 cordycepin이 세포주기 G2/M기 정체 현상을 유발하였음을 알 수 있었으며, 이는 cyclin B1 및 cyclin A의 발현 감소와 연관성이 있었다. 또한 cordycepin이 처리된 LNCap 세포에서 cyclin-dependent kinase (CDK) inhibitor p21Waf1/Cip1의 발현이 증가되었지만, CDK2, CDC2 및 Cdc25C의 발현에는 큰 영향을 미치지 않았으며, cordycepin에 의하여 증가된 p21 단백질은 CDK2 및 CDC2와의 복합체를 형성하고 있었다. 본 연구의 결과는 LNCap 전립선 암세포에서 cordycepin에 의한 G2/M 및 apoptosis 유발은 p53 비존적인 CDK inhibitor p21의 발현 증가가 중요한 역할을 하고 있음을 보여주는 것이다.

• 동의생리병리학회지
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• 제22권6호
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• pp.1518-1524
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• 2008

Ponciri Fructus (PF), the immature fruit of Poncirus trifoliata, has been used for treatment of constipation in Korean traditional medicine. It has been reported that PF has a prokinetic effect on gastrointestinal tract, but little is known about the effect on colonic contraction. The aim of this study was to investigate the effect of PF on spontaneous contractions of proximal and distal colon in rats. The aqueous extract of PF was centrifuged and filtered and its supernatant was used for in vitro motility study. The removed colon from rats was divided into proximal and distal segments. Each segment was mounted in a 10 ml organ bath and measured the change of the spontaneous contraction with increasing dose (1, 5, 10, 50, 100, 500, $1000{\mu}g/ml$) of PF extract administration. Also the effect of PF on the spontaneous contraction was measured under treatment of atropine, acetylcholine (Ach), and tetrodotoxin (TTX). PF increased the spontaneous phasic contraction of distal colon dose dependently, but there was no change in proximal colon. The contractile response induced by PF in distal colon was lower than that of Ach and was partially blocked by atropine ($10^{-6}M$). TTX increased the spontaneous contraction and it was reinforced with Ach addition. But the extract of PF had no or little contractile effect of TTX in colon. PF increased spontaneous contractions selectively in distal colon. The prokinetic effect of PF may be due to enhancement of cholinergic related excitatory neural system.

• 대한한의학원전학회지
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• 제29권2호
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• pp.13-29
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• 2016

Objectives : The purpose of this study is to investigate the concept of the Chukbu(尺膚) diagnosis in Huangdineijing(黃帝內經) and explain the characteristic of Chukbu(尺膚) diagnosis and causes of its clinical applicational decline. It will help the application of the Chukbu(尺膚) diagnosis to clinical treatment. Methods : The Sikuquanshu(四庫全書) database and Traditional Chinese Medical(TCM) books web database were used. The related contents of the Chukbu(尺膚) diagnosis in Huangdineijing (黃帝內經) and its annotation books were analyzed. The mentions on the Chukbu(尺膚) diagnosis in other medical books were examined. Results & Conclusions : The Chukbu(尺膚) diagnosis is important in the diagnostic system of the Huangdineijing(黃帝內經). The Chukbu(尺膚) diagnosis is composed of inspection and palpation. Its characteristic is something different compared to that of the Chongumaek(寸口脈) diagnosis; it relatively diagnoses condition of exterior disease(表病). The causes of its clinical applicational decline are relative inconvenience, limits of feudal society, and development of Zangfubianzheng(臟腑辨證) in that era.

• Advances in Traditional Medicine
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• 제3권4호
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• pp.196-204
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• 2003

Aqueous extract of Enicostemma littorale is reported to have antidiabetic activity. In the present investigation, we studied the effect of aqueous extract of E. littorale and its different fractions i.e., toluene, chloroform, ethyl acetate, n-butanol fractions and remaining residual fraction in streptozotocin (STZ)-induced neonatal type 2 diabetic rats. Fasting glucose and insulin levels in NIDDM were significantly (P<0.05) higher than control rats and they were significantly decreased by treatment with aqueous extract of E. littorale and its n-butanol and ethyl acetate fractions. Results of oral glucose tolerance test (OGTT) showed that aqueous extract and its n-butanol and ethyl acetate fractions significantly (P<0.05) decrease both $AUC_{glucose}$ and $AUC_{insulin}$ values in NIDDM treated groups. Insulin sensitivity $(K_{ITT})$ index of NIDDM control was significantly lower as compared to normal control and this was significantly (P<0.05) increased after treatment with aqueous extract, its n-butanol and ethyl acetate fractions. Treatment with aqueous extract of E. littorale and its n-butanol and ethyl acetate fractions lowered the elevated cholesterol and triglyceride levels observed in NIDDM rats. Treatment with aqueous extract of E. littorale and its n-butanol fraction showed significant decrease in creatinine, urea, SGPT and SGOT levels as compared to NIDDM control rats. However ethyl acetate fraction showed significant changes only in creatinine and SGOT levels, and not in the levels of urea, and SGPT as compared to NIDDM control rats. Treatment with toluene, chloroform and residual fractions of E. littorale did not produce any effect on glucose, insulin, triglyceride, cholesterol, creatinine, urea, SGPT or SGOT levels as compared to NIDDM control rats. Our data suggest that n-butanol and ethyl acetate fractions contain the active compounds which may be responsible for the above activity and associated complications in NIDDM diabetes mellitus.

• 대한한방내과학회지
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• 제25권1호
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• pp.16-27
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• 2004

목적 : 골쇄보(骨碎補) (Drynariae Rhizoma)는 한의학에서 골격계 질환의 치유력을 강화시키는 것으로 알려져 있는데, 항 virus, 항 박테리아, 항 염증 작용이 있는 것으로 보고되고 있다. 질병을 치료하기 위하여 면역 반응을 조절하는 기전에 관하여 오랫 동안 많은 관심을 기울여왔는데, 식물에서 추출한 약재들이 면역기능을 조절할 수 있는 가능성에 대하여 광범위하게 연구되었다. 이에 저자는 골쇄보(骨碎補)의 ethanol 추출물을 가지고 항 세포성과 변역 조절 기능에 대하여 연구하였다. 방법 : 사람의 혈액단핵구 (PBMC)의 배양은 thymidine 법으로 검정하고 nitric oxide (NO) 생성은 mouse macrophage RAW 264.7 세포주를 이용하였으며 IL-2, IFN 와 TNF-a 생성은 ELISA 기술로 검정하였다. 세포 증식은 FACScan으로 측정하고 세포 표면항원 CD16, CD25 및 HLA-DR 은 FITC/PE 항체로 측정하였다. 결과 : 골쇄보(骨碎補)는 mitogen (phytohaemagglutinin; PHA) 과 antigen (purified protein derivative; PPD) 에 의해 자극받은 human peripheral blood mononuclear cells (PBMCs) 의 증식을 억제하였다. 더욱이, 골쇄보(骨碎補)는 mouse 와 인간에 기원한 여러 세포들의 성장을 억제하였다. 또한, nitric oxide (NO), interleukin-2 (IL-2)와 tumor necrosis $factor-{\alpha}\;(TNF-{\alpha})$ 의 생성을 억제하였다. 한편, human PBMCs 에서 intracytoplasmic $interferon-{\gamma}\;(IFN-{\gamma})$와 cell surface markers 인 CD16, HLA-DR 의 expression은 골쇄보(骨碎補)에 의하여 영향을 받지 않았으나, CD25 expression 은 현저히 통제되었다. 결론 : 골쇄보(骨碎補) ethanol 추출물이 in vitro 에서 항 증식성과 변역 억제작용을 가지고 있다는 가능성을 의미하는 것으로 사료된다.

• Archives of Pharmacal Research
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• 제29권9호
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• pp.777-785
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• 2006

Nelumbinis Semen (NS), or lotus seed, is one of the most well-known traditional herbal medicines and is frequently used to treat cardiovascular symptoms in Korea. The anti-ischemic effects of NS on ischemia-induced isolated rat heart were investigated through analyses of changes in blood pressure, aortic flow, coronary flow, and cardiac output. The subjects in this study were divided into two groups: a control, untreated ischemia-induced group, and an ischemia-induced group treated with NS. There were no significant differences in perfusion pressure, aortic flow, coronary flow and cardiac output between the groups before ischemia was induced. The supply of oxygen and buffer was stopped for ten minutes to induce ischemia in isolated rat hearts, and NS was administered during ischemia induction. NS treatment significantly prevented decreases in perfusion pressure, aortic flow, coronary flow and cardiac output under ischemic conditions (p<0.01). In addition, the mechanism of the anti-ischemic effects of NS was also examined through quantitation of intracellular calcium content in rat neonatal cardiomyocytes. NS significantly prevented intracellular calcium increases induced by isoproterenol (p<0.01). These results suggest that NS has distinct anti-ischemic effects through calcium antagonism.