• 제목/요약/키워드: organic medium

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Biodegradation of trichloroacetic acid from organic solvent tolerant bacterium, Pseudomonas savastanoi BCNU 106

  • 김종수;박형철;조수동;이승한;김기욱;문자영;정영기;주우홍
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2003년도 생물공학의 동향(XII)
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    • pp.390-392
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    • 2003
  • Pseudomonas savastanoi BCNU 106은 mineral salt medium에서 30 mM trichloroacetic acid의 농도까지 유일한 탄소원으로 이용하여 생육할 수 있다는 것을 발견하였다. 또한 trichloroacetic acid 뿐만 아니라 monochloroacetic acid, trichloroethylene, p-dichlorobenzene을 유일한 탄소원으로 이용 가능하였다. 그러나 이들 화합물에 대한 내성시험결과 전혀 내성이 없는 것을 알게 되었다.

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관계구성 체계로서 내.외부 공간의 인터페이스적 특성에 관한 연구 (Study on the Characteristics of Interface between the Inner and Outer Space as a Relationship-forming System)

  • 고현동;이호중
    • 한국실내디자인학회:학술대회논문집
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    • 한국실내디자인학회 2006년도 춘계학술발표대회 논문집
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    • pp.234-239
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    • 2006
  • The concept of surface has become more relative and more relationship-oriented based upon interaction between spaces as the traditional realm of perception succumbed to that of a more flexible, open perspective. If surface characteristics are consideredas an interface, an interface with individual characteristics will form part of the whole and will become an element in the whole (the environment), manifesting itself as a medium incorporated in an architectural space. This authorintends to understand interface not as a mere intermediary space but as an intervening border that allows exchanges between different environments and as a medium that allows expressions of aesthetic order through organic order. Also, it is the Intention of this author to grasp extended meanings of interface.

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중소도시 공동주택의 실내공기질(새집증후군) 실측에 관한 연구 (Field Measurements of Indoor Air Quality in Apartment Units at Medium-size Cities)

  • 윤재옥
    • KIEAE Journal
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    • 제10권4호
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    • pp.81-86
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    • 2010
  • The main sources of the new house syndrome(sometimes it called sick building syndrome) are a concentration of formaldehyde (HCHO) and a concentration of total volatile organic compounds(TVOC). I had field measurements of indoor air quality in the apartment unit at medium-size cities(Y city, C city). I measured indoor air temperature, HCHO concentration in 16 units, TVOC concentration in 6 units and air tightness in 7 units. And I measured outdoor air quality, HCHO concentration and TVOC concentration. Mean concentration of HCHO was $357{\mu}g/m^3$(2006 standard=$120{\mu}g/m^3$), mean concentration of TVOC was $3,092{\mu}g/m^3$ and mean effective air leakage area was 193 cm2. There was a close relation between the indoor air temperature and HCHO concentration, between the indoor air temperature and TVOC concentration. Air tightness also had relation.

음이온 교환크로마토그래피에 의한 벤조산 및 그 유도체들의 분리에 관한 연구 (A Study on Separation of Benzoic Acid and Its Derivatives by Anion Exchange Chromatography)

  • 강삼우
    • 대한화학회지
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    • 제18권5호
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    • pp.358-362
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    • 1974
  • 벤조산과 그 유도체들에 대한 음이온 교환분리를 여러 농도의 염화니켈-메탄올 용매 내에서 연구하였다. Amberlite CG-400, $Cl^-$ form에 대한 유기산들의 부피분배계수를 측정하고 이 분배계수로부터 제시된 적절한 농도의 $NiCl_2-MeOH$ 용액을 용리액으로 사용하여 몇 개의 혼합유기산을 정량적으로 분리하였다. 모든 유기산들의 농도는 자외선 분광광도계를 사용하여 정량하였다.

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생물여과 시스템을 이용한 다성분계 휘발성 유기화합물의 분해 (Degradation of Volatile Organic Compound Mixtures Using a Biofiltration System)

  • 윤인길;박창호
    • KSBB Journal
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    • 제15권5호
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    • pp.501-506
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    • 2000
  • A bench-scale air biofilter was evaluated for the removal of volatile organic compounds (VOCs) from a gas stream. Compost and peat were used as the biological attachment media. Biofilter operating parameters such as incoming VOCs concentrations, temperature, and packing materials were examined. After 26 days of acclimation periods, at 25$^{\circ}C$ and 45$^{\circ}C$, the biofilter removed more than 90% of 30 to 72 mg/㎥ of total VOC. After 40 days of operation, the concentrations of isoprene, toluene, and m-xylene were reduced to 96∼99, 91∼93, and 91∼93% of the original concentrations. VOC removal efficiency was not affected by the temperature. The medium pH was maintained near neutral (pH 6.5∼7.1). After 37 days of operation, the total bacteria count in the biofilter media increased to 1.12${\times}$10(sup)8 cells/g of medium.

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인삼적부병원균의 영양생리학적 연구 (The Nutritional Physiology of Red Rotting of Ginseng cause by Erwinia carotovora)

  • 유병서;이민웅;이지열;최혜정
    • 미생물학회지
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    • 제20권3호
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    • pp.119-124
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    • 1982
  • As a basic study to elucidate nutritional physiology and composition of synthetic medium of red rotting bacteria, Erwinia carotovora, of ginseng, the effects of hydrogen ion concentration, various kinds of carbon sources, nitrogen source, micrometallic salts and it's concentration on the gorwth of the bacteria were investigated and the results were as follows. Optimal pH in the basal medium for the growth of the bacteria was 6.5. After incubation the pH in culture media was neutralized. Among the various kinds of carbon sources, sucrose, glucose mannitol, but organic acids were not utilized effectively as nutrients. After incubation the pH turned acidic. Alanine as organic nitrogen sources nad ammonium sulfate as inorganic nitrogen promoted the growth, but L-valine and sodium nitrite were the least effective. Ferric chloride 1.0mg/dl and ferrous sulfate 100mg/dl were the most effective as micrometallic sources. Control and boric acid were the least effective. New synthetic medium based on the above results was follows ; Alanine 1.0g, $KH_2PO_4\;1.0g, \;sucrose\;30.0g, \;MgSo_4{\cdot}7H_2O\;0.5g, \;FeCl_3{\cdot}6H_2O\;1.0mg\;thiamine\;200{\gamma}g$, and distilled water 1000ml, pH6.5.

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Involvement of Organic Acid During Corrosion of Iron Coupon by Desulfovibrio desulfuricans

  • Park, Kyung-Ran;Lee, Hyun-Jin;Lee, Hong-Keum;Kim, Yeong-Kwan;Oh, Young-Sook;Choi, Sung-Chan
    • Journal of Microbiology and Biotechnology
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    • 제13권6호
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    • pp.937-941
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    • 2003
  • Microbiologically influenced corrosion (MIC) is an electrochemical process where the participation of microorganisms initiates, facilitates, or accelerates the corrosion reaction. Sulfate-reducing bacteria (SRB) reduce sulfate to sulfide and are known to be the most destructive microorganisms in anaerobic MIC. Accordingly, the current study attempted to elucidate the mechanisms involved and the relative importance of the corrosive products in SRB-induced corrosion. The measured rate of anaerobic corrosion of iron coupons by Desulfovibrio desulfuricans was $89.9{\;}\mu\textrm{g}{\;}\textrm{m}^{-2}{\;}d^{-1}$. Direct contact between the cells and the iron coupon did not seem to be necessary for corrosion to occur, since the corrosion rate was similar ($100.8{\;}\mu\textrm{g}{\;}\textrm{m}^{-2}{\;}d^{-1}$) when the coupon was enclosed in a dialysis bag. The participation of sulfide in the corrosion process was only marginal, as the specific corrosion rate was 2.5 times higher in a sulfate-free pyruvate medium than in an $H_2S-producing$ lactate medium. Acetate (18.8-22.1 mM), the end-product of pyruvate and lactate metabolism, was identified in the culture medium and thus presumed to play a major role in the corrosion process involving Desulfovibrio desulfuricans.

Carrageenan 필름의 미생물에 의한 생분해도 측정 (The Bildegradability of Carrageenan-based Film by Microorganisms)

  • 강성국;정순택;박현진;임종환
    • 한국미생물·생명공학회지
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    • 제23권6호
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    • pp.702-709
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    • 1995
  • Degradation Of $\kappa $-carrageenan-based film by microorganisms screened from carrageenan source and activated sludge of a carrageenan producing factory was investigated by measuring changes of pH, viscosity, total sugar and total organic carbon (TOC) of the medium containing $\kappa $-carrageenan as a carbon source. Initially fifteen strains of microorganism were isolated from carrageenan source and activated sludge and then three organisms among them were selected based on the ability of growing in the medium including 0.3% $\kappa $-carrageenan as a sole carbon source. They were identified as Escherichia coli, Saccharomyces cerevisiae and Aspergillus niger. As indices of biodegradability Of $\kappa $-carrageenan based film, the changes of pH, viscosity, total sugar, and TOC of the carrageenan film-based medium were tested by the cultivation of single or mixed strains of the identified organisms. Mixed culture showed the highest biodegradability, which resulted in the changes of pH from 6.5 to 3.0, viscosity from 164 cps to 15.6 cps, total sugar content from 2.35 g/l to 0.53 g/l and TOC from 5721 ppm to 232 ppm after 30 days of cultivation. The biodegradability determined as the reduction rate of TOC by pure cultures of Asp. niger, E. coli, Sacch. cerevisiae and mixed culture of the three organisms were 94%, 86%, 80% and 96%, respectively.

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기주식물엽에 있어서 녹병균의 분화 (Rust Fungus Diferentiation on Host Leaves)

  • 김원겸
    • 미생물학회지
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    • 제13권1호
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    • pp.37-44
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    • 1975
  • As a basic study to elucidate nutritional physiology and composition of synthetic medium of red rotting bacteria, Erwinia carotovora, of ginseng, the effects of hydrogen ion concentration, various kinds of carbon sources, nitrogen source, micrometallic salts and it's concentration on the gorwth of the bacteria were investigated and the results were as follows. Optimal pH in the basal medium for the growth of the bacteria was 6.5. After incubation the pH in culture media was neutralized. Among the various kinds of carbon sources, sucrose, glucose mannitol, but organic acids were not utilized effectively as nutrients. After incubation the pH turned acidic. Alanine as organic nitrogen sources nad ammonium sulfate as inorganic nitrogen promoted the growth, but L-valine and sodium nitrite were the least effective. Ferric chloride 1.0mg/dl and ferrous sulfate 100mg/dl were the most effective as micrometallic sources. Control and boric acid were the least effective. New synthetic medium based on the above results was follows ; Alanine 1.0g, $KH_2PO_4$ 1.0g, sucrose 30.0g, $MgSo_4$ $7H_2$O 0.5g, $FeCl_36H_2$O 1.0mg thiamine 200.gamma.g, and distilled water 1000ml, pH6.5.

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Antioxidant and Antibacterial Activity of Caprylic Acid Vanillyl Ester Produced by Lipase-Mediated Transesterification

  • Kim, Jin Ju;Kim, Hyung Kwoun
    • Journal of Microbiology and Biotechnology
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    • 제31권2호
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    • pp.317-326
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    • 2021
  • Vanillyl alcohol (VA), which is abundant in Vanilla bean, has strong antioxidant activity. However, the use of VA in the food and cosmetics industries is limited, due to its low solubility in emulsion or organic solvents. Meanwhile, medium chain fatty acids and medium chain monoglycerides have antibacterial activity. We synthesized butyric acid vanillyl ester (BAVE) or caprylic acid vanillyl ester (CAVE) from VA with tributyrin or tricaprylin through transesterification reaction using immobilized lipases. BAVE and CAVE scavenged 2,2-diphenyl-1-picrylhydrazyl radicals in organic solvents. In addition, BAVE and CAVE decreased the production rate of conjugated diene and triene in the menhaden oil-in-water emulsion system. While BAVE showed no antibacterial activity, CAVE showed antibacterial activity against food spoilage bacteria, including Bacillus coagulans. In this study, the antibacterial activity of vanillyl ester with medium chain fatty acid was first revealed. Zeta potential measurements confirmed that BAVE and CAVE were inserted into B. coagulans membrane. In addition, the propidium iodide uptake assay and fluorescent microscopy showed that CAVE increased B. coagulans membrane permeability. Therefore, CAVE is expected to play an important role in the food and cosmetics industries as a bi-functional material with both antioxidant and antibacterial activities.