• Journal of the Korean Society of Environmental Restoration Technology
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• v.8 no.6
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• pp.59-68
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• 2005

This study was carried out to investigate soil properties, ordination, and vegetation of the coastal sand dune in Sinduri, Taean-Gun. 1. The Orobanche coerulescens and Ixeris repens that are a peculiar species were found in Sinduri coastal sand dune and the Salix purpurea var. japonica that is an endemic species of korea was found in that place. The plant communities was categorized into seven groups, such as Rosa rugosa community, Vitex rotundifolia communit, Carex kobomugi community, Imperata cylndrica var. koenigii community, Carex pumila community, Artemisia capillaris community, and Calamagrostis epigeios community. 2. The soil organic matter, total nitrogen, available phosphorous concentrations, and cation exchange capacity were lower in the study sites than in forest soil of seashore. There was little difference in soil exchangeable Ca, Mg, and K concentrations between in the study site and in the forest soil of seashore. The soil pH was ranged from 5.69 to 7.63. The soil texture in the study site was sand or loamy sand. 3. The results of the correlation between Sinduri coastal sand dune community and environmental factor are as follows; the soil pH was the most effect to the community distribution, and CEC, total nitrogen, soil organic matter, and the amount of silt in soil have some correlation with community distribution. 4. The results of the correlation between Sinduri coastal sand dune community and soil characteristics are as follows; the Calamagrostis epigeios community was found in area that have high soil moisture content; the Rosa rugosa community was found in area that have high soil CEC, organic matter concentration, and total nitrogen concentration, and low soil pH; the Vitex rotundifolia community, Carex kobomugi community, and Artemisia capillaris community were found in area that have low soil CEC, organic matter concentration, and total nitrogen concentration, and high soil pH; the Imperata cylndrica var. koenigii community and Carex pumila community were found in area that have medium soil CEC, organic matter concentration, total nitrogen concentration, and soil pH.

• Applied Biological Chemistry
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• v.18 no.1
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• pp.42-51
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• 1975

Penicillium sp I which produces a powerful hydrolysing enzyme was isolated from putrefid and dry Jerusalem artichoke medium. The strain was used to study on the optimum culture conditions for enzyme production. The results obtained are as follows: 1. Penicillium sp I was a vigorous strain to produce inulase. 2. The optimum culture conditions of the strain was examined in the Jerusalem artichoke extract medium and the synthetic medium. 3. Inulase productivity in the Jerusalem artichoke extract medium was higher than that of the synthetic medium. 4. The optimum culture period of the Jerusalem artichoke extract medium was four days, whereas that of the synthetic medium was five days. 5. The optimum temperature, pH and concentration in the Jerusalem artichoke extract medium were $30^{\circ}C$, 5.0 and 4.0% (W/V), respectively. Meanwhile, the optimum temperature, pH and concentration in the synthetic medium were $30{\sim}33^{\circ}C$, $5.0{\sim}6.0$, and $1.0{\sim}1.5%$ (W/V), respectively. 6. Corn steep liquor, peptone, $(NH_4)_2HPO_4,\;NH_4H_2PO_4,\;(NH_4)_2SO_4$, etc. were favorable as nitrogen sources. Of these, especially, Corn steep liquor and peptone as organic nitrogen sources caused an increase in inulase production in the synthetic medium. 7. All sugars except for inulin have no effect upon the inulase production. 8. KCl, $MgSO_4\;and\;FeSO_4$ were favourable mineral sources for inulase production.

• Journal of Korean Society for Atmospheric Environment
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• v.17 no.1
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• pp.75-84
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• 2001

Hot gas filtration method via using ceramic filters is an evolving technology applicable to numerous industrial and air pollution control processes. Alumino silicate, organic and inorganic binders were the major raw materials in manufacturing ceramic filters. In this work, disc type ceramic filters(50$\phi$$\times$10t) were manufactured by vacuum forming processes using ceramic raw materials. The porosity and bulk density of disc type ceramic filers ranged from 86 to 89% and from 0.27 to 0.36 g/㎤, respectively. In this work disc type ceramic medium were tested utilizing coupon experimental apparatus. Disc type filters showed high collection efficiencies over 99.96% with Darchs law coefficients of 4.1$\times$10(sup)10~9.63$\times$10(sup)10/$m^2$ depending on mean pore sizes. In addition, filtration and detachment of ceramic filters turned out to be performed effectively using 10 cm/sec face velocity, 5 minutes filtration cycle, 100msec pulse jet valve opening time and 3 bar pulsing pressure.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.541-545
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• 2000

For the sustained release formulation of recombinant human growth hormone (rhGH), dissociable rhGH aggregates were microencapsulated within poly(D,L-lactic-co-glycolic acid) [PLGA] microparticles. rhGH aggregates with 2 - 3 m Particle diameter were first produced by adding a small volume of aqueous rhGH solution into a partially water miscible organic solvent phase(ethyl acetate) containing PLGA. These rhGH aggregates were then microencapsulated within PLGA polymer phase by extracting ethyl acetate into an aqueous phase pre-saturated with ethyl acetate. The resultant microparticles were 2 - 3 m in diameter similar to the size of rhGH aggregates, suggesting that PLGA polymer was coated around the protein aggregates. Release profiles of rhGH from these microparticles were greatly affected by changing the volume of the incubation medium. The release rhGH species consisted of mostly monomeric form with having a correct conformation. This study reveals that sustained rhGH release could be achieved by microencapsulating reversibly dissociable protein aggregates within biodegradable polymers.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.271-274
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• 2000

A study was carried out to select a nitrogen source and the optimize the C/N ratio for the maximum cell growth of Phaffia rhodozyma in fed-batch culture. The yeast extract was the best organic nitrogen source. In the batch culture experiments, the highest cell yield was obtained 0.575 g-cell/g-glucose from 10 g/L and 10 g/L yeast extract. In the fed-batch experiments, the maximum cell concentration was obtained 33.1 g/L from the C/N ratio of 2:1 while the astaxanthin concentration of cell was Increased by increasing the C/N ratio, of feed medium.

• Journal of the Korean Wood Science and Technology
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• v.21 no.1
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• pp.7-14
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• 1993

To increase the production of mushrooms and the economic efficiency of mushroom cultivation, the pinchips(PC) and sawdusts(SD) of three Korean oak species were tested as cultivating media for Lentinus edodes with varing the composition of the media. The results are as follows. 1. Organic acids(tannic acid and citric acid), which were added in the PC medium to enrich the quality of mushrooms, did not increase the growths of the mycelia and the quality. 2. The mushrooms were able to be harvrested from six months after beginning the cultivation. No significant differences were found among the qualities of the mushrooms produced by various treatments. 3. The production rate of mushroom was relatively high with, 28.4~29.3% (700~730gr./2,500gr pack). The net profit estimated was about 1,450 won per pack.

• Proceedings of the Microbiological Society of Korea Conference
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• 2004.05a
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• pp.101-104
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• 2004

Among biological hydrogen production processes, fermentative processes have some advantages. In this research, the hydrogen producing bacterium was isolated from domestic landfill area and identified as Enterobacter sp. The strain was named Enterobacter sp. SNU-1453. Important parameters for the hydrogen process include pH, temperature, concentration of initial glucose, and kind of sugars. The pH of the culture medium significantly decreased as fermentation proceeded due to the accumulation of various organic acids, and this inhibited the $H_2$ production seriously. When pH was controlled at pH 7.0, hydrogen production was 2614.5 m1/1 in 17 hours. The increase of glucose concentration resulted in higher $H_2$ production. The productivity of this strain was 6.87 mmol $H_2/l$ per hi on concentration of 25g glucose/l. Enterobacter sp. SNU-1453 could utilize various sugars. These results indicate that Enterobacter sp. SNU-1453 has a high potential as a fermentative $H_2$ producer.

• Journal of Ginseng Research
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• v.14 no.2
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• pp.107-111
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• 1990

Ginseng root explants and calli were cultured on modified Murashine and Skoog's media supplemented with different concentrations of organic or inorganic compounds and plant growth requlators to clarify the effects of chemical compositon and plant growth regulators in the medium on the growth of ginseng calli and the production of ginseng saponin. For optimum growth of ginseng calli, the concentrations of 2, 4-D and sucrose were in the range of 1 to 5 mg/l and 1 to 3%, respectively. And it was clarified that sucrose, nitrogen, phosphate, calcium, magnesium, plant growth regulators and their concentrations influcenced the relative biosynthesis of saponin in tissue cultures of Panax ginseng.

• Journal of Microbiology and Biotechnology
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• v.18 no.6
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• pp.1115-1120
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• 2008

A novel cathepsin B inhibitor-producing bacterium was isolated from marine sediments and identified based on its 16S rDNA sequence as Pseudomonas sp. strain PB01 (Accession No. EU126129). The growth and enzyme inhibitor production were investigated under various culture conditions. A mixture of organic nitrogen source was required for the optimal production, whereas both glucose and maltose proved to be the effective carbon sources for cathepsin B inhibitor production. Other optimal culture conditions included temperature range between 25 and $28^{\circ}C$, initial medium pH of 6.6, and shaking speed of 200 rpm. Under these optimal conditions, the maximum inhibitory activity from culture broth was approximately 50% after 30 h of cultivation. Additionally, kinetic study revealed that inhibitor production paralleled with cell growth, which suggested that the inhibitor may be a primary metabolite of that bacterium.

• Advances in materials Research
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• v.3 no.2
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• pp.117-128
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• 2014

Processable conducting polyaniline derivative with free amine functional groups was successfully synthesized from the monomer o-phenylenediamine in aqueous hydrochloric acid medium using ammonium persulfate as an oxidative initiator. The synthesized poly(o-phenylenediamine) (PoPD) in critical condition was found to be completely soluble in common organic solvents like dimethyl sulfoxide, N,N-dimethyl formamide etc. From the intrinsic viscosity measurement, the optimum condition for the polymerization was established. The polymer was characterized by ultraviolet visible spectroscopy, Fourier transform infrared spectroscopy, proton magnetic resonance spectroscopy ($^1HNMR$) and thermogravimetric (TGA) analyses. The weight average molecular weights of the synthesized polymers were determined by the dynamic light scattering (DLS) method. From the spectroscopic analysis the structure was found to resemble that of polyaniline derivative with free amine functional groups attached to ortho/meta position in the phenyl ring. However, very little ladder unit was also present with in the polymer chain. The moderate thermal stability of the synthesized polymer could be found from the TGA analysis. The average DC conductivity of $2.8{\times}10^{-4}S/cm$ was observed for the synthesized polymer pellet after doping with hydrochloric acid.