• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.112-112
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• 2022

This study aims to confirm the possibility of using the invasive alien plants in Jeju as a functional biomaterial. To achieve this purpose, 70% ethanol extract and solvent fractions were prepared for five invasive alien plants (Hypochaeris radicata, Rumex acetosella, Humulus japonicus Siebold & Zucc., Solanum viarum, Lactuca scariolar) and their antioxidant, antibacterial anti-inflammatory and anti-obesity effects were investigated. The DPPH radical scavenging activity of ethanol extract from invasive alien plants was shown in the order of Rumex acetosella > Hypochaeris radicata > Humulus japonicus. Antimicrobial activity of ethanol extract against food poisoning bacteria (4 species) and oral cavity-induced microorganisms (6 species) was measured. As a result, the extract of Humulus japonicus showed high antibacterial effects against food poisoning bacteria (E. coli, V. parahaemolyticus) and oral microbes (L. casei, S. epidermidis, E. faecalis). In LPS-induced RAW 264.7 cells, the anti-inflammatory effect of ethanol extract from invasive alien plants was investigated. As a result, the NO production inhibition activity was highest in the Rumex acetosella and the Humulus japonicus Siebold & Zucc. ethanol extract, and the NO production inhibition activity was concentration-dependent. In addition, the Rumex acetosella and the Humulus japonicus Siebold & Zucc. ethanol extract showed a concentration-dependent inhibitory effect on cytokine (IL-6) production. These extracts also showed inhibitory activity of COX-2, an inflammatory protein. This suggests that NO production inhibition activity by the extract of invasive alien plants is the result of inhibition of iNOS and COX-2 expression. Currently, organic solvent fractions of crude extract are manufactured and the investigation of active ingredients is continuing along with evaluation of biological activity such as anti-inflammatory. These results are expected to be a major data for the study on the separation and utilization of active ingredients with antioxidant, antibacterial and anti-inflammatory effects using foreign plant crude extract and solvent fractions, and are highly likely to be applied to the development of functional food and cosmetics materials.

• 한국유기농업학회지
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• 제25권2호
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• pp.445-460
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• 2017

본 연구는 국내 단감 유기재배 선도농가의 병해충 방제 실태조사, 주요 병해충에 대한 친환경 방제자재 선발, 수립한 방제시스템의 현장실증시험을 2013년부터 2015년까지 3년 동안 수행하였다. 유기재배 선도농가의 주 재배품종은 "부유"였으며, 병해충 방제를 연간 9~17회 유기농업 자재를 살포하였다. 피해가 가장 심한 병해충은 탄저병, 노린재, 감꼭지나방, 깍지벌레 등이었으며, 사용한 방제자재는 결정석회황합제, 황토유황, 유화제, 보르도액 그리고 마늘, 은행, 소나무를 이용한 자가제조 식물추출물제를 이용하였다. 탄저병 방제에 효율적인 자재는 결정석회황, 황토유황이었고, 마늘유+ 시트로넬라유제, 고삼종자 추출물+차추출물제가톱다리개미허리노린재와 독나방에 효과적이었다. 감꼭지나방 교미교란트랩을 60개/10a 설치하면 감 착과율을 30% 향상시켰다. 유기재배 단감에 연간 10회 방제하는 방제시스템의 현장실증시험 결과 70.7%의 과일을 수확할 수 있었다. 그러나, 수확기 즈음인 9월, 10월에는 약제를 살포할 수 없기 때문에 이때 가해하는 노린재류의 피해는 감소하지 않았으며, 추가적인 연구가 필요하다.

• Journal of Microbiology and Biotechnology
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• 제9권1호
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• pp.39-43
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• 1999

A flocculent aggregate produced by Lactococcus lactis subsp. lactis in broths containing Tween 80, including MRS broth, had a microscopic structure of intertwined thread-like filaments. The filamentous structure was not elongated bacterial cells, but consisted of an organic solvent-soluble portion and an insoluble solid. L. lactis subsp. lactis grown at $25^{\circ}C$ for 15 days in tryptic soy broth with 0.1% Tween 80 and 1.0% malt extract produced 13 mg/l of flocculent aggregate, which contained 0.84 g/g of organic solvent-soluble component. The organic solvent-soluble part was identified as 10-hydroxyoctadecanoic acid.

• 동아시아식생활학회지
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• 제15권1호
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• pp.65-70
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• 2005

This study was conducted in order to promote the utilization of Hovenia dulcis Thunb as food. The physicochemical properties and sensory characteristics of the extracts from the leaf, fruit, stalk and stem were examined. The extract of stem was the brightest in L value (p<0.05), but the lowest in b value (p<0.01). By the sensory evaluation, the external appearance was the best in the extract of stem. The extract of leaf was the darkest in color and that of stem the lightest. The bitter and astringent taste was stronger from the leaf compared with other parts. The extract of fruits stalk of Korean Hovenia dulcis was preferred in most of attributes. The total sugar content was the highest from the fruits stalk. Among free amino acids, aspartic acid, glutamic acid and alanine were rich in order. Potassium contents were the highest among minerals from the fruits stalk, leaf and stem. Ca and Mg followed the next. The results were the same both in Korean and in Chinese tree. Among organic acids, malic acid was the most in the fruits stalk, leaf and stem. It was followed by citric acid. In the test of free sugar, glucose was the most and followed by sucrose and fructose in the fruits stalk. Fructose was contained the most in leaf and stem.

• Journal of Electrochemical Science and Technology
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• 제7권4호
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• pp.251-262
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• 2016

The corrosion protection of mild steel in 1M HCl and 0.5M $H_2SO_4$ solutions by ethanol extract of Vernonia amygdalina (VA) was studied using a combination of experimental and computational methods. The obtained results revealed that VA reduced the corrosion of mild steel in both environments and inhibition efficiency increased with VA concentration but decreased with prolonged exposure. Electrochemical results showed that the extract functioned via mixed corrosion inhibiting mechanism by adsorption of some organic constituents of the extract on the metal/acid interface. Findings from infrared spectroscopy and electron microscopy all confirmed that VA retarded mild steel corrosion in both 1M HCl and 0.5M $H_2SO_4$ through an adsorption process. The adsorption behavior of selected constituents of the extract was modeled using density functional theory computations.

• Biomolecules & Therapeutics
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• 제2권2호
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• pp.149-154
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• 1994

Anti-allergic and anti-inflammatory actions of the water extract from Cimicifuga heracleifolia were evaluated in mice and rats. Several criteria were employed to assess the anti-allergic and anti-inflammatory actions of Cimicifuga heracleifolia, such as hyaluronidase activity, mediators-induced vascular permeability changes, 48 hour homologous passive cutaneous anaphylaxis (PCA) histamine release from mast cells, and the carrageenan-induced rat paw edema. To further characterize the active components, the water extract was either extracted with organic solvent or fractionated according to molecular weight, and each fraction was tested for some of anti-allergic parameters. Hyaluronidase activities, both in activating and in activated states, were significantly inhibited by the water extract of Cimicifuga heracleifolia and by some of its subfractions, molecular weight less than 1,000. The water extracts (50~400 mg/kg) significantly inhibited 48 hr homologous PCA and vascular permeability changes induced by chemical mediators (histamine, serotonin, and leukotriene $C_4$) in mice. In the case of histamine-induced vascular permeability changes, more extensive studies were conducted; water extract was either fractionated according to molecular weight or extracted with butanol. Anti-histamine actions were observed only from the water layer, and these active components were of the molecular weight less than 1,000. These anti-allergic actions were observed mainly from mice than from rats. On the other hand, anti-inflammatory actions of the water extract from Cimicifuga heracleifolia were significant in rats.

• 한국응용과학기술학회지
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• 제41권2호
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• pp.520-529
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• 2024

Lycium barbarum extract has a high potential to be developed as a health functional food due to the various health-promoting effects of Lycium barbarum. This study analyzed changes in nutritional and functional components depending on the extraction solvent (purified water and a mixture of purified water and alcohol) and the condition of the sample. The nutritional components (carbohydrates, protein, fat, ash), organic acids, amino acids, total phenolic compounds, and total flavonoids of the extract produced during the extraction process were analyzed. The nutritional composition and functional substances of the extracts showed some differences depending on the type of solvent and the condition of the sample. The amounts of crude protein (7.61%), crude fat (1.63%), carbohydrate (90.22%), and ash (0.54%) of dried Lycium barbarum extract using purified water as a solvent were similar to those of the powder sample extract. The highest content of citric acid was 4.31 mg/mL, similar to the case of acetic acid, when the powder sample used a mixture of purified water and alcohol as a solvent. The highest amino acid content was 357.39 mg/mL when the powder sample was mixed with purified water and alcohol as a solvent. The total amount of phenolic compounds was 686.16 g/L when the powder sample was extracted with a mixture of purified water and alcohol as a solvent. The highest total flavonoid content was 111.32 g/L when the powder sample was extracted with a mixture of purified water and alcohol as a solvent.

• 한국유기농업학회지
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• 제23권2호
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• pp.311-322
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• 2015

본 연구는 개여뀌를 이용하여 친환경 농자재로 개발하기 위한 기초자료를 제공하기 위해 수용성 추출액의 농도에 따른 재배작물인 감자생육에 미치는 알레로패시 효과를 조사하였다. 감자 시험구 처리는 개여뀌추출물 무처리, 25%, 50%, 75%, 100%로 하였으며, 개여뀌추출물의 처리는 정식 1일 후부터 5일 간격으로 처리구에 따라 관주하였다. 개여뀌의 수용성 추출액농도가 증가함에 따라 대조구에 비하여 맹아가 완료되는 시기가 1일이 빨랐고, 경수는 0.3-0.4/1주 개가 많았다. 경장은 생육초기에는 개여뀌추출물 처리구가 무처리구에 비해 컸으나, 생육 중반 이후에는 차이를 보이지 않았으며, 경직경 및 지상부 생체중은 개여뀌 처리구가 무처리구에 비해 양호한 경향을 보였으나 통계적 유의성은 없었다. 감자의 총수량/$m^2$은 개여뀌를 처리한 100%구에서 $13.8kg/m^2$로 가장 많았으며 처리간에 유의차가 인정되었다. 감자의 크기별 개수는 4 g 이하, 5-30 g 미만의 괴경 수량은 각 처리간에 일정한 경향을 보이지 않았으나 31 g 이상의 괴경 수량은 무처리구에 비해 개여뀌 수용성 추출물 처리구가 현저히 높았다. 이들 결과를 종합하면 개여뀌의 allelopathy 효과는 감자의 생육과 수량형질이 촉진되어 친환경 비료로써 활용 가능성을 가지고 있는 것으로 판단된다.

• 한국식품저장유통학회지
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• 제25권1호
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• pp.71-78
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• 2018

본 연구는 건강에 대한 소비자들의 관심이 증대됨에 따라 영양분 섭취와 질병 예방 차원에서 각종 과실과 이를 활용한 제품 개발의 일환으로 베리류 중 블랙커런트 농축액을 요구르트 대체 0, 5, 10, 15, 및 20% 비율로 첨가하여 요구르트 드레싱의 품질특성과 항산화성을 분석하였다. 일반성분 분석에서 블랙커런트 첨가량이 증가할수록 요구르트 드레싱의 조회분과 탄수화물은 증가하고 수분함량, 조단백, 조지방은 감소하는 경향을 나타내었다. pH와 점도는 블랙커런트 첨가량이 증가됨에 따라 유의적으로 감소하는 결과를 보였으며(p<0.001) 당도는 블랙커런트의 당도로 인하여 첨가량과 비례하여 증가하는 결과를 보였다. 색도는 블랙커런트 첨가량이 증가할수록 L 값은 감소하였으며, a 값과 b 값은 증가하였다. 블랙커런트 첨가 요구르트 드레싱의 유기산 결과 citric acid, malic acid, succinic acid, acetic acid는 블랙커런트 첨가량이 증가할수록 높게 나타났으며, tartaric acid와 lactic acid는 감소하는 것으로 나타났다. 요구르트의 항산화능에서 총 페놀 함량은 대조군이 가장 낮았으며, 블랙커런트 첨가량이 증가할수록 높게 나타났다. 안토시아닌의 경우 대조군이 0.62 mg/100 mg로 가장 낮았으며 블랙커런트 20%첨가 요구르트 드레싱이 190.09 mg/100 mg로 유의적으로 높게 나타났다(p<0.001). DPPH radical 소거능과 ABTS도 블랙커런트 첨가 요구르트 드레싱군이 대조군보다 높게 나타났으며 아질산염 소거능 또한 블랙커런트 첨가량이 증가할수록 높게 나타나 블랙커런트를 첨가함으로써 요구르트 드레싱의 항산화 효과를 증진시킬 수 있을 것으로 사료된다.

• Journal of the Korean Wood Science and Technology
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• 제40권2호
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• pp.123-132
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• 2012

Pre-pulping extracts were found to contain a dilute amount of xylo-oligosaccharides and acetic acid as the major components, and many minor components including other organic acids, lignin-derived phenolics, and sugar degradation products. Once separated from the pulp, a secondary hydrolysis step was required to hydrolyze oligomeric hemicellulose sugars into monomeric sugars before fermentation. The following study detailed the extent of hemicellulose recovery by pre-pulping using hot water extraction and characterized the hydrolysis of the extract with respect to comparing acid and enzymatic hydrolysis. The secondaryhydrolysis of hot water extracts made at an H-Factor of 800 was tested for a variety of acid and enzyme loading levels using the sulfuric acid and xylanases. The maximum fermentable sugar yield from acid and enzyme hydrolysis of the extract was 18.7 g/${\ell}$ and 17.7 g/${\ell}$ representing 84.6% and 80.1% of the maximum possible yield, respectively.