• 제목/요약/키워드: organic and fatty acids

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쇠비름(Portulaca oleracea L.)에 함유(含有)된 생리활성물질(生理活性物質) 탐색(探索) (Identification of Physiologically Active Compounds from Purslane(Portulaca oleracea L.))

  • 박지성;김길웅
    • 한국잡초학회지
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    • 제8권2호
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    • pp.169-175
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    • 1988
  • 밭 및 비농경지(非農耕地)의 우점잡초(優占雜草)인 쇠비름에 함유(含有)되어 있는 생이활성물질(生理活性物質)의 탐색(探索)을 통(通)하여 얻어진 몇가지의 결과(結果)를 요약(要約)하면 다음과 같다. 쇠비름을 건조(乾燥)한 시료(試料)로부터 추출(抽出)한 수용(水溶) 및 methanol 추출액(抽出液)은 상치, 무우 및 비름 등(等)의 식물종자(植物種子) 발아(發芽)를 크게 억제(抑制)시켰다. 쇠비름에 함유(含有)된 phenol 화합물(化合物)은 주(主)로 umbeliferon, p-coumaric, salicylic, vanillic, p-hydroxybenzoic, tannic, gallic산(酸) 등(等)이었다. 쇠비름의 건체(乾體), 생체(生體), 종자(種子) 및 callus 상태(狀態) 시료(試料)에 함유(含有)된 phenol 화합물(化合物)을 비교(比較)하면 건체시료(乾體試料)에서는 gallic산(産) 생체시료(生體試料)에서는 salicylic, vanillic산(酸), 종자시료(種子試料)에서는 ferulic산(酸), callus에서는 p-hydroxybenzoic, p-chlorobenzoic, cinamic산(酸) 등(等)이 많이 함유(含有)되어 있어 시료간(試料間)에 차이(差異)가 있었다. 지방산(脂肪酸)은 건체(乾體), 생체(生體) 및 종자시료(種子試料)에 linolenic산(酸)이 각각(各各) 9.203, 14.186, 1.732 mg/g으로 많이 함유(含有)되어 있었고 callus에는 palmitic산(酸)이 1.601 mg/g으로 많았다. 그리고 유기산(有機酸) 가운데는 oxalic산(酸)이 건체(乾體), 생체(生體) 및 callus 시료(試料)에서, citric산(酸)은 callus 시료(試料)에서 많았다.

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Patterns of Plasma Fatty Acids in Rat Models with Adenovirus Infection

  • Paik, Man-Jeong;Park, Ki-Ho;Park, Joong-Jean;Kim, Kyoung-Rae;Ahn, Young-Hwan;Shin, Gyu-Tae;Lee, Gwang
    • BMB Reports
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    • 제40권1호
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    • pp.119-124
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    • 2007
  • Adenoviral vectors are among the most promising vectors available for human gene therapy. However, the use of recombinant adenoviral vectors, including replicationcompetent adenovirus (RCA), raises a variety of safety concerns in relation to the development of new therapies based on gene therapy. To examine how organic compounds change in rat plasma following the injection of adenovirus, $\beta$-galactosidase expressing recombinant adenovirus (designated rAdLacZ) or RCA, we investigated the content of fatty acids (FAs), which are important biochemical indicators in pathological conditions. Pattern recognition analysis on the level of FAs in rat plasma is described for the visual discrimination of adenovirus infection groups from normal controls. Plasma FAs from four control rats (normal group), and from four rats with rAdLacZ infection and six rats with RCA infection (the two abnormal groups), were examined by gas chromatography-mass spectrometry in selected ion monitoring modes as their tert-butyldimethylsilyl derivatives. In total, 20 FAs were positively detected and quantified. The results of the Student's t-test on the normal mean of two abnormal groups, the levels of three FAs (p<0.05) from rAdLacZ group and eleven FAs (p<0.05) from RCA group were significantly different. When star symbol plotting was applied to the group mean values of 20 FAs after normalization to the corresponding normal mean values, the resulting eicosagonal star patterns of the two infected groups were distorted into similar shapes, but were distinguishable from each other. Thus, these approaches will be useful for screening and monitoring of diagnostic markers for the effects of infection following the use of adenoviral vectors in gene therapy.

헛개나무잎차의 이화학적 특성 (Physiocochemical Properties of Hovenia dulcis Thunb. Leaf Tea)

  • 정창호;배영일;심기환
    • 한국식품저장유통학회지
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    • 제7권1호
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    • pp.117-123
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    • 2000
  • In order to promote the utilization of Hovenia dulcis leaf as food, leaf tea was nanufactured and its physicochemical properties were examined. Aong proximate composiotns of Hovenia dulcis leaf tea were found in both fermented tea and roasted tea the higher contents of total sugar and crude protein. In minerals component of fermented tea and roasted tea, potassium (K) was the most high amounts of 11,560.6 ppm and 11,084.6 ppm, respectively. The highest contents of free sugar in both teas were mainly consisted of sucrose, the amounts of sucrose showed 1.47% and 1.94% . In case of organic acids, oxalic acid and citric acid were revealed 631.26mg% and 660.05mg%, respectively. The highest contents of total amino acid in fermented tea and roasted tea were 812.01mg% and 709.72mg% of glutamic acid. the fatty acids of fermented tea and roasted tea were mainly composed of 40.18% of linolenic acid and 26.92% of palmitic acid, respectively. The major volatile compounds of fermented tea and roasted tea were composed of 3, 7, 11, 15-tertramethyl-2-hexadecen-1-ol and methyl palmitate and the amounts of those showed 290.1ppm and 472.97 ppm, respectively. The tannins of fermented tea and roasted tea were 1.02% and 1.26% , as the extraction steps increased the tannin contents decreased. In Hunter's color values of tea extract L and b values decreased , while a value of those increased as the extraction steps were repeated.

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목단줄기를 접목시킨 작약근과 목단피의 생태적 특성조사 및 성분분석에 의한 품질 비교 (Comparison of Quality and Ecological Properties Radix and Moutan Cortex, which were Paeonia Radix Grafted with Moutan Stem)

  • 장기운;최강주;고성룡;김현경
    • Applied Biological Chemistry
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    • 제45권3호
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    • pp.173-178
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    • 2002
  • 약용식물의 생산량을 증가시키고 지상부 꽃은 관상용으로 활용할 수 있는 연구의 일환으로 목단 줄기에 작약 뿌리를 접목시켜 생육생태를 관찰하고 유효지표성분외에 무기성분, 지방산, 유기산등 성분변화 및 향미 관능검사등을 비교한 결과 생육생태를 볼 때 지상부는 목단줄기에 목단꽃이 피었으며 지하부 뿌리는 작약뿌리와 목단뿌리가 혼재하여 생성되었으며, 작약뿌리와 목단뿌리의 혼재비율은 약 3:1정도로 나타났다. paeoniflorin및 paeonol 분석결과 건조시킨 작약근의 함량은 대건물 시료(g)중 9.91 mg/g및 0.80 mg/g,접목 작약근은 11.71 mg/g 및 0.35 mg/g, 목단피는 10.81 mg/g 및 2.84 mg/g, 접목 목단피는11.26 mg/g 및 3.28 mg/g으로 유사한 경향이었다. 무기성분별 10종 함량은 대체로 유사한 경향이었으나 Fe함량은 시료별로 차이가 있었으며 작약근은 86.06 mg/g, 접목 작약근 127.36 mg/g, 목단피 75.05 mg/g, 접목 목단피는 140.63 mg/g으로 함량 차이가 현저하였다. 지방산은 linoleic acid, palmitic acid, linolenic acid 및 oleic acid등이 주성분으로 총 11종을, 유기산은 oxalic acid, citric acid 및 malic acid등이 주성분으로 총 6종을 각각 분석하였으나 함량 조성은 대체로 유사한 경향이었다. 한편, 향미 관능평가 결과 작약근과 접목 작약근은 대체로 유사한 향미 패턴을 나타내었으나, 작약근은 작약근 향미 및 떫은맛이 다소 강하였으며, 목단피와 접목 목단피도 거의 유사한 향미를 나타내었으나, 목단피가 목단피 향미, 쓴 맛, 화한 맛 및 떫은 맛이 다소 강하였다.

LANGMUIR-BLODGETT 박막 증착장치 제작 및 전반사감쇠법에 의한 Phenylhydrazone (Construction of Langmuir trough and optical properties of phenylhydrazone dye LANGMUIR-BLODGETT films by attenuated total reflection)

  • 고해석
    • 한국광학회:학술대회논문집
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    • 한국광학회 1990년도 제5회 파동 및 레이저 학술발표회 5th Conference on Waves and lasers 논문집 - 한국광학회
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    • pp.193-198
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    • 1990
  • We constructed Langmuir trough for the ultra-thin films (from a few tens {{{{ ANGSTROM }}) of nonlinear optical organic materials. Surface pressure - area isotherm for the tetracosanoic acid which is a fatty acids, was recorded and Langmuir -Blodgett films was deposited using it. The homogenity was investigated using scanning electron microscopy (SEM).

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EFFECT OF UREA SUPPLEMENTATION ON THE NUTRITIVE VALUE OF OAT SILAGE

  • Azim, A.;Nadeem, M.A.;Khan, A.G.
    • Asian-Australasian Journal of Animal Sciences
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    • 제5권1호
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    • pp.51-54
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    • 1992
  • Silages were prepared with different ratios of oat and wheat straw 100:0, 80:20, 60:40, 40:60, 20:80, and 0:100 with or without urea supplementation and were ensiled for 60 days in air tight polyethylene bags and kept at room temperature. Results showed that dry matter loss was maximum in 100% oat silage and it decreased as the ratio of wheat straw increased. There was a significant difference in crude protein value of the silages prepared by different ratios of oat and wheat straw. Addition of urea significantly increased the crude protein and decreased the crude fibre value. Silage made with urea supplementation were well fermented as indicated by higher value of volatile fatty acids (VFA) and lower value of residual carbohydrates. Maximum VFA concentrations were observed in 60:40 oat-wheat straw silages. The value of enzyme soluble organic matter (ESOM) was high in all the urea supplemented silages as compared to without urea and maximum value was found in 60:40 oat-wheat straw combination.

신구저수지의 1차 생산 및 지방산 생성속도 결정을 위한 $^{13}C$ 추적자 활용 연구 (An Application of $^{13}C$ Tracer for the Determination of Primary Productivity and Fatty Acid Production Rate in Shingu Reservoir)

  • 이연정;김민섭;신경훈;황순진
    • 생태와환경
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    • 제41권spc호
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    • pp.21-26
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    • 2008
  • 본 연구는 $^{13}C$추적자를 사용하여 신구저수지 내 식물플랑크톤에 의한 1차 생산속도를 계산하고, $^{13}C$-GC/MS 기법을 사용하여 광합성을 통해 새롭게 만들어진 지방산의 생성속도를 분석하였다. 유광층 내의 SPM중 POM이 차지하는 비율은 약 76%였으며, 엽록소$-{\alpha}$의 평균 농도는 약 $89{\mu}g\;L^{-1}$이었다. 유광층 단위면적당 엽록소$-{\alpha}$ 총량, 1차 생산력, 엽록소$-{\alpha}$에 의한 탄소 고정 능력은 각각 112mg Chl-${\alpha}m^{-2}$, 3.53g C $m^{-2}\;d^{-1}$, 32mg C mg Chl-${\alpha}^{-1}\;d^{-1}$이었다. 수층 내 입자성유기물의 지방산 조성과 식물플랑크톤 광합성에 의해 새롭게 만들어진 입자성유기물의 지방산 조성이 거의 비슷한 것으로 미루어 보아, 본 연구기간 동안 수층의 입자성유기물은 대부분 식물플랑크톤에 의해 유래된 것으로 판단되었다. 또한 광합성을 통해 만들어진 유기물은 세포외배출 등의 과정을 통해 용존유기물 pool로 이동된 후 박테리아에게까지 빠르게 전달됨으로써, 박테리아에 의한 유기물 소비가 활발하게 일어나고 있는 것으로 판단된다.

해양부산물 아미노산액비 및 유용미생물(KEM) 시용이 들깻잎의 품질에 미치는 영향 (Effect of Seafood Amino Acid Fertilizer and Korean Effective Microorganisms on the Leaf Quality of Perilla frutescens var. japonica)

  • 조전권;안승원;김영칠;황인수;김명선;이정관;노희영
    • 한국환경과학회지
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    • 제19권10호
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    • pp.1301-1305
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    • 2010
  • This study was carried out to investigate the effect of KEM and SAF appication on contents change of fatty acids and organic acid of perilla(Perilla Frutescens Britton). Contenst of squalene in perilla leaves on control and KEM/SAF treated were 3.39 mg and 4.22 mg, respectively. Therefore the squalene quantity of KEM/SAF treated leaves was 24.2% more than that of control. A total 6 fatty acids in perilla leaves were analyzed in this study. Percentage of the saturated and unsaturated fatty acid in perilla leaves were 20 and 80%, respectively. Contents of phytosterols in perilla leaf such as campesterol and sitosterol were 2.0 and 20.0 mg, respectively. Therefore sitosterol content was 10 fold more than that of campesterol. The KEM/SAF application on perilla leaf was effective on the change of squalene or phytosterol contents. However effect of that was negligible on the change of fatty acid content.

Chemical Composition and Phytoestrogen Analysis of Iranian Black Pomegranate Juice Concentrate and Seeds

  • Choi, One-Kyun;Kim, Yong-Seong;Yu, Hye-Kyoung;Lee, Chan;Bang, Hyo-Pil;Yang, Deok-Chun;Kim, Young-Kee
    • Plant Resources
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    • 제6권1호
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    • pp.27-35
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    • 2003
  • In this study, as preliminary research for the development of natural estrogen supplement the chemical properties of Iranian black pomegranate juice concentrate and seeds were evaluated. Proximate compositions of pomegranate juice concentrate and seeds were as follows; crude lipid 0.4% and 8.2%, moisture 39.9% and 6.6%, crude protein 0.9% and 12.2%, ash 1.4% and 1.7%, and carbohydrate 42.0% and 84.5% respectively. Major amino acids are glutamic acid (1310.0ppm) and aspartic acid (896.2ppm) in juice concentrate, and glycine (611.1ppm) and arginin (401.6ppm) in seeds. Ascorbic acid has the highest concentration of 20.0mg/l00g in juice concentrate and 0.23mg/l00 in seeds. The compositions of unsaturated fatty acids such as linoleic acid and linolenic acid were higher than those of saturated fatty acids such as stearic palmitic acid. Major minerals were potassium, calcium and sodium, potassium was highest in both juice concentrate and seeds. Vitamins were composed of ascorbic acid (20.0mg/l00g), vitamin B$_1$(0.12mg/100g) and niacin (0.80mg/l00g) in juice concentrate, and only ascorbic acid(0.23mg/l00g) in seeds. Organic acids such as citric and L-malic acid were detected only in pomegranate juice concentrate. The contents of total polyphenols were 4.55g/L in juice concentrate and 3.5mg/l00g in seeds, respectively. Phytoestrogens detected in pomegranate juice concentrate and seeds were daidzein, quercetin, genistein and 17 $\beta$-estradiol.

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유기지방산 신규악취물질에 대한 저온농축 열탈착방식 (Thermal desorber)의 검량특성 연구 (A study on the calibration characteristics of organic fatty acids designated as new offensive odorants by cryogenic trapping-thermal desorption technique)

  • 안지원;김기현;임문순;주도원
    • 분석과학
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    • 제22권6호
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    • pp.488-497
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    • 2009
  • 이 연구에서는 2010년 기준의 지정악취물질에 해당하는 유기지방산과 함께 기존 VOC류 지정 악취물질들을 포괄적으로 분석할 수 있는 흡착관-열탈착 분석기법을 연구하였다. 이러한 분석기법의 검량특성을 평가하기 위해, Tenax TA를 충전한 시료흡착관을 준비하였다. 그리고 여기에 유기지방산 4종 (propionic acid (PA), butyric acid (BA), isovaleric acid (IA), valeric acid (VA))을 위시한 13 가지 조사성분을 함유한 작업용 표준시료를 주입하고 저온농축기법으로 분석하였다. 유기지방산의 분석감도를 절대량 (ng) 및 농도기준 (ppb)의 검출한계로 비교해 보았을 때, 다른 일반적인 VOC 악취성분들에 비해 약 1.5-2배 낮게 나타나는 것을 확인하였다(PA: 0.24 ng (0.16 ppb), BA: 0.19 ng (0.11 ppb), IA: 0.15 ng (0.07 ppb), and VA: 0.28 ng (0.13 ppb)). 분석의 재현성을 RSE로 표시할 경우, 대부분의 성분들이 5% 이내로 안정적으로 나타난 반면, BA (8.02%), IA (14.0%), VA (5.08%)는 5%가 넘을 정도로 낮은 재현성을 보였다. 이러한 결과를 내부표준물질을 이용한 검량으로 비교하였을 때, 대다수 성분의 재현성이 향상되는 양상을 보였다(PA: $1.1{\pm}0.4%$, BA: $10{\pm}0.46$, IA; $12{\pm}0.3%$, VA: $4{\pm}0.1%$). 이 연구의 결과, 악취공정시험법에서 유기지방산의 분석방법으로 권장한 알칼리 흡수법에 대비하여 흡착튜브를 이용한 시료의 채취가 보다 유용한 대체방법으로 활용 가능한 것으로 나타났다.