• 한국식품영양학회지
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• 제10권3호
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• pp.295-301
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• 1997

토양으로부터 분리한 120 여종의 집락 중 응집제를 생산하는 집락을 선발하고 가장 응집력이 우수한 K-67 균주를 최종 선발하여 Agrobacterium sp. KF-67로 동정하고 실험균주로 사용하였다. 응집제 생산시 최적 배지성분을 조사하기 위하여 응집활성을 kaolin과 활성탄 현탁액에서 응딥활성을 측정하였다. 최적 배지성분과 농도는 탄소원과 무기질소원은 glucose와 NH4NO3 각각 2%와 0.1%, 유기질소원은 yeast extract와 peptone 각 0.01%, 무기염으로 CaCO3을 0.04%를 첨가하였을 때 응집활성이 가장 우수하였다. 배지 중 0.03% NaCl 첨가로 약 19%의 응집활성을 향상시킬 수 있었다. 이상과 같은 결과로 볼 때 Agrobacterium sp.에 의한 응집제 생산시 최적배지의 성분 조성과 함량은 2% glucose, 0.1% NH4NO3, 0.01% yeast extract, 0.01% peptone, 0.04% CaCO3, 0.03% NaCl이었고, 이때 기초배지에 비해 약 76.1%의 응집활성력이 증가하여 응집제 생산에 효과적인 배지성분 조건으로 조사되었다.

• Journal of Plant Biotechnology
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• 제30권1호
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• pp.65-71
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• 2003

본 실험은 그늘쑥을 Agrobacterium을 통해 형질전환체인 모상근을 유기하고 모상근의 대량배양을 위해 모상근이 가장 잘 자라는 생장 조건을 찾기 위해 수행되었다. 실험 결과를 종합해 보면 선발된 그늘쑥 모상근 세포주의 대량 생산을 위한 최적 배지는 MS 기본 배지임을 확인하였다. 또한 최적의 havesting time 모상근의 성장률이 좋고 갈변과 배지의 미생물 오염도가 적은 4주가 적당한 것으로 판단된다. 그리고 광 처리 하에서 모상근을 배양하는 것보다는 암상태에서 모상근을 키우고 pH는 5.8 정도가 탄소원은 sucrose로 3.5%가 모상근 을 대량배양시 최적조건임을 확인되었다. 미량원소인 Fe$^{2＋}$은 0.1 mM을 처리하는 것이 모상근의 성장을 촉진하였으며 spermine과 spermidine은 각각 10 mM와 100 mM로 처리하는 것이 모상근의 성장을 높일 수 있다. 앞으로 연구는 이 결과를 토대로 모상근 대량 배양을 위한 scale up조건과 bioreator의 설계에 관한 연구가 필요하다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.289-293
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• 2003

Schizophyllum commune의 균체 생육 및 다당체 생성에 미치는 여러 가지 배지성분의 영향을 조사하였다. 탄소원으로는 산업적인 경제성과 높은 다당체 생성량을 나타낸 glucose를 선택하였고, 무기질소원은 유기질소원에 비해 현저히 낮은 다당체 생성량을 나타내었으며, 그 결과 균체량과 다당체 생성량에서 높은 효과를 나타낸 yeast extract를 질소원으로 선택하였다.

• 설비공학논문집
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• 제13권7호
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• pp.612-620
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• 2001

The optimum design of a heat exchanger with porous media insertion is studied in this paper. It is considered that the aluminum foam metal is inserted in a flat plate channel and air flows through it. The influence of the microstructure of the foam metal on the pressure drop and heat transfer is investigated utilizing previous analytical results and existing correlation equations. Design parameters are identified as the unit-cell size and the ligament thickness of the porous medium, and their effects are examined. The results show that there exists optimum microstructure of the porous media maximizing heat transfer with a constant pressure drop. When the increase in the pressure drop is within a practically acceptable range, the increase in the heat transfer is dominated by the increase in the heat transfer area due to the porous medium insertion. Consequently, among the porous media with a constant pressure drop, the heat transfer is maximized with a microstructure with maximum specific surface area.

• Design & Manufacturing
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• 제9권2호
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• pp.10-15
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• 2015

The water has been the suitable for the cooling medium until now. But the water as cooling medium seem to have the limit for high speed injection. The steam plastic molding injection use the steam as the medium when raise the mold temperature. The weld line has been the major quality problems in a plastic injection parts to be difficult to be solved. These problems in injection-molded plastic parts are difficult to find the reason because these issues are usually in tradeoff realtions with each other. The purpose of this paper is to obtain the optimum injection moulding condition for improving the quality of plastic injection parts and to inquire the productivity improvement with the measured cycle time by steam plastic moluding injection. Based on these numerical results, the guidelines of mould design and injection processing condition were established. As a result, the improvement of quality and the reduction of cycle time was achieved.

• Applied Biological Chemistry
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• 제18권1호
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• pp.42-51
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• 1975

돼지감자 시료(試料)(Putrefactived and dry Jerusalem artichoke)로 부터 강력(强力)한 Inulin가수분해(加水分解) 효소(酵素)를 생산(生産)하는 Penicillium sp. I을 분리선발(分離選拔)하였고 본균(本菌)을 사용(使用)하여 효소생산(酵素生産)에 관(關)한 최적배양조건(最適培養條件)을 검토(檢討)하여 다음과 같은 결론(結論)을 얻었다. 1. Penicillium sp. I은 Inulase생산력(生産力)이 강(强)한 균주(菌株)이다. 2. 본균(本菌)의 Inulase생산(生産)을 위한 최적배양(最適培養) 조건(條件)을 Jerusalem artichokoke extract media와 Basal media의 두 배양기(培養基)에서 결정(決定) 하였다. 3. Jerusalem artichoke extract media에서 Basal media보다 Inulase생산력(生産力)이 높았다. 4. Inulase생산능(生産能)도 Jerusalem artichoke extract media에서는 4일(日) Basal media에서는 5일(日) 배양(培養)으로써 최고치(最高値)에 도달하였다. 5. Inulin농도(濃度), pH. 온도조건(溫度條件) Jerusalem artichoke extract media에서는 opt. temp $30^{\circ}COpt.$ pH 5.0, Opt. conc 4.0%(W/V)로 나타나고 Basal media에서는 Opt temp $30{\sim}33^{\circ}C$, Opt. pH $5.0{\sim}6.0$, Opt conc $1.0{\sim}1.5%(W/V)$ 6. 질소원(窒素源)으로서는 Corn Steep Liquir, Peptone, $(NH_4)_2HPO_4,\;NH_4H_2PO_4,\;(NH_4)_2SO_4$등이 량호(良好)하고 특히 유기질소원(有機窒素源)을 가(加)함으로써 Basal meaia에서 Inulase생산(生産)을 증가(增加)시켰다. 7. 당원(糖源)의 이용(利用)으로써는 Inulin이외(以外)의 제당(諸糖)은 Inulase생산(生産)에 영향(影響)을 미치지 않았다. 8. 금속(金屬) 염류(鹽類)의 이용(利用)은 KCI. $MgSO_4,\;FeSO_4$등이 Inulase생산(生産)을 증가(增加)시켰다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.207-209
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• 2005

Serum is a potential source of bacterial, mycoplasmal and viral contamination, and it has a possibility of the introduction of serum proteins, prion and pyrogens into the final vaccine product. For porcine Rotavirus vaccine production, it is necessary to develop serum free medium which do not cause those problems. A new serum free medium was developed for porcine Rotavirus vaccine based on DMEM, and the performance of developed serum free medium was evaluated in terms of Vero cell growth and Rotavirus vaccine production. The cell density, gown in serum free medium developed, was similar with that in serum supplemented medium. Also, it was higher than that in other commercially available serum free medium. The productivity of Rotavirus vaccine using serum free medium developed and optimum production strategies will be also discussed.

• 동아시아식생활학회지
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• 제21권5호
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• pp.661-668
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• 2011

This study was conducted in order to determine the optimum conditions for the production of fermented grapefruit extract showing high cell growth, antioxidant activity and total flavonoid content. Five lactic acid bacterial strains - Lactobacillus plantarum KCTC3104, Lactobacillus brevis KCTC3102, Weisella cibaria KCTC3746, Leuconostoc citreum KCTC3526 and Leuconostoc mesenteroides KCTC3505 - were evaluated first in order to determine the optimum strain able to grow with high efficiency on grapefruit as a substrate and possesses higher antioxidant activity and flavonoids content. Among these strains, L. mesenteroides KCTC3505 was selected as a starter culture. To estimate the available or effective content of grapefruit in basal medium, the effects of 30%, 50%, and 70% grapefruit contents on the performance of fermentation were tested, and it was found that grapefruit can be added at 70% levels to medium. In this study, three factors of fermentation conditions - incubation time, sucrose, and glucose contents - were evaluated for their effects on fermentation performance. Taguchi experiment design was employed and the responses of experiments were calculated using signal and noise ratio calculation with larger-the-best characteristics. Finally, the optimum conditions for the manufacture of fermented grapefruit extract were as follows: grapefruit 70%, sucrose 10 g/L, glucose 10 g/L, sodium acetate 1 g/L, NaCl 1 g/L, dipotassium phosphate 0.1 g/L, magnesium sulfate 0.01 g/L and 16 hr of incubation.

• 한국균학회지
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• 제23권3호통권74호
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• pp.275-283
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• 1995

The nutritional requirements of mycelial growth for Pleurotus spp. were studied. As the carbon sources, soluble starch and dextrin, nitrogen sources, arginine and ammonium tartrate, and calcium sources, $CaCO_3$ enhanced mycelial growth. Optimum C/N ratio was 100 : 1. On the base of results from the experiment on nutritional requirements, the following receipe is suggested for growth of Pleurotus spp.; starch 15 g, arginine 3.484 g, ammonium tartrate 3.06 g, $CaCO_3$ 0.314 g, $KH_2PO_4$ 8.138 g, $K_2HPO_4$ 1.584 g, $MgSO_4{\cdot}7H_2O$ 1.5 g for P. sajor-caju and 2.5 g for P. ostreatus. $FeSO_4{\cdot}7H_2O$ 0.02 g, $ZnSO_4{\cdot}7H_2O$ 0.03 g, $MnSO_4{\cdot}7H_2O$ 0.02 g, optimum pH 6.0. This new synthetic medium is tentatively designated as Park's medium.

• Journal of the Korean Wood Science and Technology
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• 제19권3호
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• pp.27-34
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• 1991

Screw withdrawal test was carried out on the face and edge of domestic particleboard and medium density fiberboard in order to evaluate optimum pilot hole diameter. The obtained results were as follows: 1. Maximum withdrawal strengths on the face and edge of particleboard were obtained with pilot hole diameters at about 50% of root diameters of screw. 2. Maximum withdrawal strength on the face and edge of medium density fiberboard were obtained with pilot hole diameters that were about 60% and 50% of root diameters of screw, respectively. 3. Withdrawal strength showed about 91% of maximum withdrawal strength when pilot holes were not pre bored at particleboard. but when pilot holes at 90% of root diameter of screw withdrawal strength showed about as 51.3% of maximum withdrawal strength. 4. Withdrawal strength showed about 88% of maximum strength when pilot holes were not used, but withdrawal strength indicated 55.4% of maximum strength in case of 90% of root diameters of screw. 5. Maximum withdrawal strength on the face of particleboard was about 70.5% higher than that of the edge, and however medium density fiberboard was about 19.6% higher than that of the edge.