• Microbiology and Biotechnology Letters
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• v.30 no.4
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• pp.305-311
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• 2002

Acetyl xylan esterase gene (AXE) from Aspergillus ficuum was cloned and its Pichia expression plasmid, pPICZ$\alpha$C-AXE (4.6 kb), was constructed, in which the AXE gene was under the control of the AOXI promoter and connected downstream of mating factor u-1 signal sequence. The plasmid linearized by Sacl was integrated into the 5'AOXI region of the chromosomal DNA of P. pastoris. In the flask batch culture of P. pastoris transformant on methanol medium, the cell concentration and total AXEase activity reached at 6.0 g-dry cell weight/1 and 77 unit/ml after 36 h cultivation, respectively. In the fed-batch culture employing the optimized methanol and histidine feeding strategy, the cell concentration and total AXEase activity were significantly increased to about 97 g-dry cell weight/1 and 930 unit/ml. Most of AXEase activity (>90%) was found in the extracellular medium and the majority of extracellular protein (>80%) was AXEase enzyme (33.5 kDa). This result means that about 9.8 g/1 of AXEase protein was produced in the extracellular medium.

• Journal of Communications and Networks
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• v.4 no.4
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• pp.351-362
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• 2002

In this paper, we introduce a new approach to the minimum energy routing (MER) for next generation (NG) multihop wireless networks. We remove the widely used assumption of deterministic, distance-based channel model is removed, and analyze the potentials of MER within the context of the realistic channel model, accounting for shadowing and fading. Rather than adopting the conventional unrealistic assumption of perfect power control in a distributed multihop environment, we propose to exploit inherent spatial diversity of mobile terminals (MT) in NG multihop networks and to combat fading using transmit diversity. We propose the cooperation among MTs, whereby couples of MTs cooperate with each other in order to transmit the signal using two MTs as two transmit antennas. We provide the analytical framework for the performance analysis of this scheme in terms of the feasibility and achievable transmit power reduction. Our simulation result indicate that significant gains can be achieved in terms of the reduction of total transmit power and extension of network lifetime. These gains are in the range of 20-100% for the total transmit power, and 25-90% for the network lifetime, depending on the desired error probability. We show that our analytical results provide excellent match with our simulation results. The messaging load generated by our scheme is moderate, and can be further optimized. Our approach opens the way to a new family of channel-aware routing schemes for multihopNG wireless networks in fading channels. It is particularly suitable for delivering multicast/ geocast services in these networks.

• Journal of the Korean Institute of Telematics and Electronics
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• v.22 no.5
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• pp.29-38
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• 1985

In this paper we have designed and implemented in real time a simple, efficient and flexible AOPCM cosec using a high speed digital processor, NEC 7720. For ADPCM system, we have used an instantaneous adaptive quantizer and a first-order fixed predictor. The software for NEC 7720 has been developed and it was found that the NEC 7720 was capable of performing the entire ADPCAt algorithm for 4 channels in real time as optimizing the program. Computer simulation has born made to investigate a computational accuracr of NEC 7720 and to de-termine necessary parameters for a ADPCM codec. Real telephone speech, RC-shaped Gaussian noise and 1004 Hz tone signal were used for simulation. In simulation, the parameters werc optimized from the computed SNR and the informal listening test. The developed software was tested in real time operation using a hardware emulator for NEC 7720. It took a maximum 23.25$\mu$s to encode one sample and 113.5$\mu$s, including all the necessary 1/0 operations, to encode 4 channels. In the case of decoding process, it took 24.75$\mu$s to decode one sample and 119.5$\mu$s to decode 4 channels.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.18 no.12
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• pp.3029-3034
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• 2014

The paper describes the scleral diagnostic system implementation for human eyes by using the scleral color code and vessels sign pattern code generations. The system is based on the high performance DSP image signal processor, programmable gain control for preprocessing and RISC SD frames storage. RGB image signals are optimized by PGC, the edge image is detected form the gray image converted. The processing algorithms are executed by scleral color code generation and scleral vessels sign pattern code creation for discriminating and matching. The scleral symptomatic color code is generated by YCbCr values at memory map tolerated and the vessel sign pattern code is created by digitizing the 24 clock and 13 ring zones, overlay matching and tolerances. The experimental results for performance are that the system runs 40ms, and the color and pattern for diagnostic errors are around 20% and 24% on average. The system and technique enable a scleral diagnosis with subdividing the patterns and patient database.

• Journal of the Korean Society for Railway
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• v.18 no.5
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• pp.426-438
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• 2015

Train speed controlling systems are now changing from wayside systems to onboard signaling systems. Locomotive engineers refer to wayside markers to decide on a braking point when the train speed appears to be lower than the current speed. However, in the onboard signaling systems that have been installed recently, the braking point is not determined by the wayside signal but by an onboard value. In this paper, we studied braking points and methods for deciding on such points by engineers using the onboard systems. An optimized braking point is proposed via simulation of decelerating velocity to control the velocity in the signaling system through a predefined point; Gaussian distributions are used to simulate the actual situation. We estimated and demonstrated how to obtain braking parameters in order to satisfy the interval of permitted error.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.34 no.6
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• pp.363-370
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• 2021

In this paper, we presented an optimized crack and flexural strength analysis of a glass-fiber reinforced polymer (GFRP) rebar, used as reinforcements for in-site railway concrete slabs. The insulation performance of a GFRP rebar has the advantage of avoiding the loss of signal current in an audio frequency (AF) track circuit. A full-scale experiment, and three-dimensional finite element simulation results were compared to validate our approaches. Parametric numerical results revealed that the diameters and arrangements of the GFRP rebar had a significant effect on the flexural strength and crack control performances of the concrete track slabs. The results of this study could serve as a benchmark for future guidelines in designing more efficient, and economical concrete slabs using the GFRP rebar.

• The Korean Journal of Mycology
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• v.47 no.4
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• pp.359-371
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• 2019

To optimize the expression and secretion of ferritin protein associated with ion storage in the mushroom, Pleurotus eryngii, a recombinant secretion vector, harboring the ferritin gene, was constructed using a pPEVPR1b vector under the control of the CaMV 35S promoter and signal sequence of pathogen related protein (PR1b). The ferritin gene was isolated from the T-Fer vector following digestion with EcoRI and HindIII. The gene was then introduced into the pPEVPR1b secretion vector, and it was then named pPEVPR1b-Fer. The recombinant vector was transferred into P. eryngii via Agrobacterium tumefaciens-mediated transformation. The transformants were selected on MCM medium supplemented with kanamycin and its expression was confirmed by SDS-PAGE and western blotting. Expression of ferritin protein was optimized by modifying the culture conditions such as incubation time and temperature in batch and 20 L airlift type fermenter. The optimal conditions for ferritin production were achieved at 25℃ and after incubating for 8 days on MCM medium. The amount of ferritin protein was 2.4 mg/g mycelia, as measured by a quantitative protein assay. However, the signal sequence of PR1b (32 amino acids) seems to be correctly processed by peptidase and ferritin protein may be targeted in the apoplast region of mycelia, and it might not be secreted in the culture medium. The iron binding activity was confirmed by Perls' staining in a 7.5% non-denaturing gel, indicating that the multimeric ferritin (composed of 24 subunits) was formed in P. eryngii mycelia. Mycelium powder containing ferritin was tested as a feed additive in broilers. The addition of ferritin powder stimulated the growth of young broilers and improved their feed efficiency and production index.

• Journal of the Institute of Convergence Signal Processing
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• v.15 no.2
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• pp.70-75
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• 2014

Recently, the demand for high-integrated, low-powered, and high-powered SoC design has been increasing due to the multi-functionality and the miniaturization of digital devices and the high capacity of service informations. With the rapid evolution of the system, the required hardware performances have become diversified, the FPGA system has been increasingly adopted for the rapid verification, and SoC system using the FPGA and the ARM core for control has been growingly chosen. While the AXI bus is used in these kinds of systems in various ways, it is traditionally designed with AXI slave structure. In slave structure, there are problems with the CPU resources because CPU is continually involved in the data transfer and can't be used in other jobs, and with the decreased transmission efficiency because the time not used of AXI bus beomes longer. In this paper, an efficient AXI master interface is proposed to solve this problem. The simulation results show that the proposed system achieves reductions in the consumption clock by an average of 51.99% and in the slice by 31% and that the maximum operating frequency is increased to 107.84MHz by about 140%.

• Journal of the Institute of Convergence Signal Processing
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• v.25 no.2
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• pp.94-99
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• 2024

Recently, wind power has been gaining attention as a highly efficient renewable energy source, leading to various technological developments worldwide. Typically, wind power is operated in the form of large wind farms with many wind turbines installed in areas rich in wind resources. However, in developing countries or regions isolated from the power grid, off-grid small wind power systems are emerging as an efficient solution. To efficiently operate and expand off-grid small-scale power systems, the development of real-time monitoring systems is required. For the efficient operation of small wind power systems, it is essential to develop real-time monitoring systems that can actively respond to excessive wind speeds and various environmental factors, as well as ensure the stable supply of produced power to small areas or facilities through an Energy Storage System (ESS). The implemented system monitors turbine RPM, power generation, brake operation, and more to create an optimal operating environment. The developed small wind power system can be utilized in remote road lighting, marine leisure facilities, mobile communication base stations, and other applications, contributing to the development of the RE100 industry ecosystem.

• Korean Chemical Engineering Research
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• v.47 no.5
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• pp.558-564
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• 2009

POF(Polymer optical fiber) offers advantages of lighter, inexpensive, and easier to use over GOF(glass optical fiber). Its higher transmission loss and low bandwidth, however, make it suitable only for short distance networking such as LAN. The polymer materials and its synthesis technology of low transmission loss and the broader application for flexible POF are the two of many critical areas to be investigated more. In the current study, low-noise POF modules are developed and optimized with a low noise amplifier and low cost LED of 650 nm. In order to demonstrate the dynamic characteristics of the POF module for optical communication and sensing, we have built an image transfer module, optical transmission speed measurement module, optical transceiver for RS-232, and sound-transfer module, and the signal characteristics of them are evaluated. It is found that the module can be readily used for a quick and simple measurement of optical transfer speed. With help of analog amplifier, LED, and PD, sound and image transfers through a maximum 60 m optical waveguide have been confirmed. Real-time data transfer was also demonstrated in PID control, which is thought to be valuable to industrial plant design and control.