• Journal of Microbiology and Biotechnology
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• v.24 no.10
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• pp.1413-1420
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• 2014

Phytate is an antinutritional factor that impacts the bioavailability of essential minerals such as $Ca^{2+}$, $Mg^{2+}$, $Mn^{2+}$, $Zn^{2+}$, and $Fe^{2+}$ by forming insoluble mineral-phytate salts. These insoluble mineral-phytate salts are hydrolyzed rarely by monogastric animals, because they lack the hydrolyzing phytases and thus excrete the majority of them. The ${\beta}$-propeller phytases (BPPs) hydrolyze these insoluble mineral-phytate salts efficiently. In this study, we cloned a novel BPP gene from a marine Pseudomonas sp. This Pseudomonas BPP gene (PsBPP) had low sequence identity with other known phytases and contained an extra internal repeat domain (residues 24-279) and a typical BPP domain (residues 280-634) at the C-terminus. Structure-based sequence alignment suggested that the N-terminal repeat domain did not possess the active-site residues, whereas the C-terminal BPP domain contained multiple calcium-binding sites, which provide a favorable electrostatic environment for substrate binding and catalytic activity. Thus, we overexpressed the BPP domain from Pseudomonas sp. to potentially hydrolyze insoluble mineral-phytate salts. Purified recombinant PsBPP required $Ca^{2+}$ or $Fe^{2+}$ for phytase activity, indicating that PsBPP hydrolyzes insoluble $Fe^{2+}$-phytate or $Ca^{2+}$-phytate salts. The optimal temperature and pH for the hydrolysis of $Ca^{2+}$-phytate by PsBPP were $50^{\circ}C$ and 6.0, respectively. Biochemical and kinetic studies clearly showed that PsBPP efficiently hydrolyzed $Ca^{2+}$-phytate salts and yielded myo-inositol 2,4,6-trisphosphate and three phosphate groups as final products. Finally, we showed that PsBPP was highly effective for hydrolyzing rice bran with high phytate content. Taken together, our results suggest that PsBPP has great potential in the animal feed industry for reducing phytates.

• Korean Chemical Engineering Research
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• v.58 no.3
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• pp.369-380
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• 2020

Most mono-crystalline silicon ingots are manufactured by the Czochralski (Cz) process. But If there are oxygen impurities, These Si-ingot tends to show low-efficiency when it is processed to be solar cell substrate. For making single-crystal Si- ingot, We need Czochralski (Cz) process which melts molten Si and then crystallizing it with seed of single-crystal Si. For melts poly Si-chunk and forming of single-crystalline Si-ingot, the heat transfer plays a main role in the structure of Cz-process. In this study to obtain high-quality Si ingot, the Cz-process was modified with the process design. The crystal growth simulation was employed with pulling rate and rotation speed optimization. Studies for modified Cz-process and the corresponding results have been discussed. The results revealed that using crystal growth simulation, we optimized the oxygen concentration of single crystal silicon by the optimal design of the pulling rate, rotation speed and melt charge level of Cz-process.

• Journal of Korea Water Resources Association
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• v.44 no.9
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• pp.711-720
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• 2011

SWAT (Soil and Water Assessment Tool) model was calibrated for the flow rate of the Deachung lake with a large area of 3108.29 $km^2$. Application of SWAT model requires significant number of input data and is prone to result in uncertainties due to errors in input data, model structure and model parameters. The SUFI-2 (Sequential Uncertainty Fitting Ver. 2) program and GLUE (Generalized Likelihood Uncertainty Estimation) program in SWAT-CUP (SWAT-Calibration and Uncertainty Program) are used to select the best parameters for SWAT model. Optimal combination of parameter values was determined through 2,000 iterative SWAT model runs. The Nash-Sutcliffe values and $R^2$ values were 0.87 and 0.89 respectively indicating both methods show good agreements with observed data successfully. RMSE and MSE values also showed similar results for both programs. It seems the SWAT-CUP has a great practical appeal for parameter optimization especially for large basin area and it also can be used for less experienced SWAT model users.

• Korean Journal of Soil Science and Fertilizer
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• v.39 no.3
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• pp.157-162
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• 2006

Objective of this research was to evaluate optimal conditions of arsenic adsorption in water by zero-valent iron (ZVI). Batch experiment showed that adsorption of arsenic by ZVI followed a Langmuir isotherm model. The masses of As(V) adsorbed onto ZVI were increased as decreasing pH of the reacting solution (pH 3: 2.05, pH 5: 1.82, pH 7: 1.24, pH 9: 1.03 mg As/g $Fe^0$) and as increasing the temperature ($15^{\circ}C$ : 1.59, $25^{\circ}C$ : 1.81, 35 : $1.93^{\circ}C$ mg As/g $Fe^0$). The SEM and EDS (energy dispersive X-ray spectrometer) analysis of morphology and structure of ZVI before and after reacting with arsenic in water revealed that a relatively smooth and large surface of ZVI was transformed into a coarse and small surface particle after the reaction. The EDS spectra on the chemical composition of ZVI demonstrated that arsenic was incorporated into ZVI by adsorption mechanism. The XRD analysis also identified that the only peak for $Fe^0$ in the ZVI before the reaction and confirmed that $Fe^0$ was transformed into $Fe_2O_3$ and FeOOH, and As into $FeAsO_4{\cdot}2H_2O$.

• Composites Research
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• v.26 no.5
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• pp.289-294
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• 2013

A thinner is used to improve the multi-walled carbon nano-tube (CNT) and carbon black (CB) dispersion in a polymer matrix and to make a soft electrode. The electrical and mechanical properties of the soft electrodes are investigated as functions of CNT, CB and thinner content. The optimal mixing condition for the electrode is thinner 80, CNT 3.5, CB 18 (phr) on the basis of matrix (KE-12). The specific resistance of that is 73 (${\Omega}{\cdot}cm$), and tensile strength, tensile modulus, and elongation of that is 0.45 MPa, 0.21 MPa, and 184%, respectively. Also, a simple structure of the actuator with an optimized electrode and elastomer is fabricated and its characteristic is evaluated. At the operating voltage 25 kV, the displacement of an elastomer KE-12 is 2.24 mm, and that of an elastomer KE-12 with thinner 50 (phr) is 4.05 mm. It shows a higher displacement compared to that of 3M 4910 which has similar modulus. The actuator made with elastomer and electrode of the same material (KE-12) may have advantages for fatigue life and application.

• Journal of the Mineralogical Society of Korea
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• v.24 no.1
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• pp.37-42
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• 2011

Magnetic properties at low-temperatures can diagnose the presence of certain magnetic minerals in rocks. At the Verwey transition temperature ($T_v$, ~105~120 K), magnetite transforms from monoclinic to cubic structure as the temperature increases. At the isotropic point ($T_i$, ~135 K), magnetocrystalline anisotropic constant of magnetite passes through zero (from negative to positive) as the temperature decreases so that its optimal remanence acquisition axis changes from [111] to [001]. A sharp remanence drop was observed at $T_v$ during warming of LTSIRM (low-temperature saturation isothermal remanent magnetization). For cooling of RTSIRM (room-temperature saturation isothermal remanent magnetization), the remanence decreased on passing $T_i$ and $T_v$. On warming of RTSIRM, remanence recovery becomes more prominent as the average grain size of magnetite increases. In summary, the SIRM memory decreases with increasing grain size of magnetite. A similar, but rather gradual, remanence transition occurs for natural samples due to contribution of cations other than Fe. As a non-destructive tool, low-temperature magnetic behavior is sensitive to unravel the magnetic remanence carriers in terrestrial rocks or meteorites.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.7
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• pp.10-18
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• 2013

With advancing of mobile communication services and commercializing of VoLTE (Voice of LTE), it is getting to pay attention on QoS of VoLTE. This paper proposes an integrated E-model in which some factors influenced to service quality of VoIP and VoLTE based voice communication system are considered in calculating the voice quality of Wideband Codec. The model aims to calculate R value which reflects the situations of access network, network characteristics, terminals' usage and mobility. We mainly deal with the integrated E-model's structure, related algorithms and optimal parameters for VoLTE. Some experiments show that the voice quality difference between VoIP and VoiceChecker, and VoLTE and POLQA, is below 10%. With the proposed model, we can calculate the voice quality by making use of the factors directly affected to service quality and the environment of VoLTE terminal and network. As a result, we can estimate the service quality in advance, without measuring it in real wireless environment.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.19 no.2 s.72
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• pp.195-202
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• 2006

Thin-walled structures are efficiently utilized an automobiles, aircraft, satellite and ship as well as needed light weight simultaneously. This paper presents new shape of automobile hood reinforcement that rotating parts as engine, transmission are protected by thin-walled structures. The automobile hood is concerned about the resonance occurs due to the frequency of the rotating parts. The hood must be designed by supporting the stiffness of design loads and considering the natural frequencies. Hence, it is sustained the stiffness and considered the vibration by resonance. It is deep related to ride. Therefore, the topology, shape and size optimization methods are used to design the automobile hood. Topology technique is applied to determine the layout of a structural component optimum size with maximized natural frequency by volume reduction. In this research, The optimal structure layout of an inner reinforcement of an automobile hood for the natural frequency of a designated mode is obtained by using topology optimization method. The optimum size and the optimum shape are determined by PLBA(Pshenichny-Lim-Belegundu-Arora) algorithm.

• Journal of Digital Contents Society
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• v.19 no.3
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• pp.505-512
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• 2018

Internet demand has increased rapidly, and Internet Protocol Version 4, the main Internet protocol, can not cope with various types of network demand in the future. Therefore, it is urgent to spread IPv6-based stable network to solve the shortage of Internet address resources. Due to the proliferation of IoT and the increase in mobile devices, efficient and optimal IPv6 routing design is indispensable. In this paper, we have studied about characteristics of IPv6, address system of IPv6, structure analysis and efficient routing technology among IPv6 terminals. Furthermore, experimental results on routing packet analysis and convergence time are presented in network - based IPv6 routing implementation environment. In addition, we show the results of the experiment analysis by dividing the implementation of IPv6 routing, operation test, and fail over performance into three kinds of routing protocol methods. As a result, this study can be applied to the introduction of IPv6 system, test bed environment construction, and network design.

• Journal of the Korean Data and Information Science Society
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• v.24 no.2
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• pp.303-310
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• 2013

Forty two female students whose fat mass ratio was over 30% were participated in the experiment of fat mass loss of two treatments for 8 weeks. They kept diary for foods they ate every day, took a picture of the foods, transmitted the picture to the experimenter by the camera phone. Among those, 28 students took the picture by regular camera phone (Treatment A), and the other students used smart phone (Treatment B). Fat mass weight and its related variables had been measured repeatedly four times at an interval of two weeks during 8 weeks. It was shown from mixed model analysis of repeated measurements data that AR(1) covariance matrix was selected as the optimal covariance matrix pattern. The correlation between two successive times is highly correlated as 0.838. Based upon the AR(1) covariance matrix structure, the students using smart phones were somewhat more effective in losing fat mass weight than the students using regular camera phones. The time effect was highly significant, but the treatment-time interaction effect was insignificant. The baseline effect and total cholesterol were found to be significant, but the calories with taking foods were somewhat significant, but the waist to hip ratio was found to be insignificant.