• Proceedings of the Optical Society of Korea Conference
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• 2003.02a
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• pp.16-17
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• 2003

수 십 ∼ 수 마이크로 크기의 미세 입자에 강하게 집속된 빔을 산란시키게 되면 입자들은 운동량의 변화에 따라 광의 초점부근에서 포획되는 힘을 받게 된다. 이런 힘은 scattering force와 gradient force로 구분할 수 있고, Optical tweezers는 광의 gradient force를 이용하여 미세입자를 포획하고 조작하는 기술이다. 광에 의해 물리적인 접촉 없이 입자를 포획할 수 있다는 사실로부터 optical tweezers는 생물학을 비롯한 많은 분야에서 유용한 도구로 사용되어지고 있다. (중략)

• Proceedings of the Optical Society of Korea Conference
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• 2008.02a
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• pp.453-454
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• 2008

femtosecond laser를 광원으로 하는 optical tweezers는 광포획 뿐만 아니라 비선형 현상을 발생시킬 수 있다는 장점을 가지고 있다. 그러나 높은 첨두 출력에 의하여 포획된 세포는 쉽게 손상되어 질 수 있다. 본 논문에서는 LTRS(Laser Tweezers Raman Spectroscopy)를 통하여 femtosecond laser와 CW laser에 의한 optical tweezers 상에서의 optical damage를 비교, 분석하였다.

• Proceedings of the Optical Society of Korea Conference
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• 2002.07a
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• pp.108-109
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• 2002

Optical tweezers는 광압(radiation pressure)을 사용하여 입자들을 포획하거나 조절할 수 있다는 점에서 마이크로스케일의 유전체구뿐만 아니라 세포에서도 널리 사용되고 있다. 일반적으로 빛이라는 것은 광자들의 집합체로서 광자의 입자성으로 인하여 외부의 물체와 충돌시 운동량을 전달하게 되고 이것을 광압(radiation pressure)이라고 하며 optical tweezers [1]는 이 광압을 이용한 방법중 하나이다. 레이저빔을 입자에 집속 시켜 주게 되면 입자는 광압에 의해서 gradient force와 scattering force의 힘을 받게 된다. (중략)

• Proceedings of the Optical Society of Korea Conference
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• 2000.02a
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• pp.228-229
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• 2000

1970년 Ashkin이 레이저 광압을 이용하여 수 마이크로미터 크기(micrometer sized)의 유전체를 광속의 진행 방향으로 가속시킴과 동시에 광속축(beam axis)방향으로 입자를 끌어당기는데 성공함으로써 레이저를 이용한 미세구(micro-particle) 의 포획 및 조작에 대한 연구와 실험이 시작되었다$^{[1]}$ . 이후에 많은 사람들에 의해 연구가 활발히 이루어졌으며$^{[2]~[7]}$ , 이러한 레이저를 이용한 미세구의 포획방법은 광집게(optical tweezer)로써 생물학과 물리학 분야에서의 높은 가능성 때문에 지금도 연구가 계속되고 있다. (중략)

• Molecular & Cellular Toxicology
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• v.3 no.1
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• pp.19-22
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• 2007

Optic tweezer is powerful tool to investigate biologic cells. Of eukaryotic cells, it was poorly documented regarding to optic trapping to manipulate yeasts. In preliminary experiment to explore yeast biology, interferometric optical tweezers was exploited to trap and manipulate budding yeasts. Successfully, several budding yeasts are trapped simultaneously. We found that the budding direction of the daughter cell was almost outward and the daughter cell surrounded by other yeasts grows slowly or fail to grow. Thus it was assumed that neighboring cells around budding yeast may be critical in budding and the growth of daughter cells. This is first report pertaining to the pattern of yeast budding under the optical trap when multiple yeasts were trapped.

• Journal of the Korean Society of Visualization
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• v.12 no.2
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• pp.13-17
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• 2014

In the present study, the optofluidic droplet manipulation in a microfluidic platform was demonstrated via theoretical and experimental approaches. Optical scattering force and gradient force were used to separate and trap droplets. Two types of droplets were generated by a T-junction method in the microfluidic channel. While they approach a test region where the optical beam illuminates the droplets, they were pushed by the optical scattering beam. The displacement by the laser beam is dependent on the refractive index of the droplets. By using the optical gradient force, the droplets can be trapped and coalesced. In order to bring the droplets in a direct contact, the optical gradient force was used to trap the droplets. A theoretical modeling of the coalescence was derived by combining the optical force and drag force on the droplet.

• International Journal of Oral Biology
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• v.43 no.2
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• pp.53-59
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• 2018

In order to understand biological phenomena accurately, single molecule techniques using a physical research approach to molecular interactions have been developed, and are now widely being used to study complex biological processes. In this review, we discuss some of the single molecule methods which are composed of two major parts: single molecule spectroscopy and manipulation. In particular, we explain how these techniques work and introduce the current research which uses them. Finally, we present the oral biology research using the single molecule methods.

• Korean Journal of Optics and Photonics
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• v.11 no.3
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• pp.141-146
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• 2000

Mie particles such as polystylene latex spheres with $3~5{\mu}m$ diameters are easily trapped and freely moved toward an arbitrary position by using the low power He-Ne laser beam focussed by the objective lens with the magnification of 40 X or 100 X. By using this technology, we can successfully arrange Mie particles in the form of the capital letter A. As a result, we can confirm the possibility of the optical tweezer that can readily trap and freely move the Mie particles. icles.

• Korean Journal of Microbiology
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• v.53 no.2
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• pp.71-78
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• 2017

Raman microspectroscopy is a promising tool for microbial analysis at single cell level since it can rapidly measure the cell materials including lipids, nucleic acids, and proteins by measuring the inelastic scattering of a molecule irradiated by monochromatic lights. Using Raman spectra provides high specificity and sensitivity in classification of bacteria at the strain level. In addition, a Raman approach coupled with stabled isotope such as $^{13}C$ and $^2H$ is able to detect and quantify general metabolic activity at single cell level. After bacterial detection process by Raman microspectroscopy, interested unculturable cell sorting and single cell genomics can be accomplished by combination with optical tweezer and microfluidic devices. In this review, the characteristics and applications of Raman microspectroscopy were reviewed and summarized in order to provide a better understanding of microbial analysis using Raman spectroscopy.